Tatsuji Hataya

Hokkaido University, Sapporo, Hokkaidō, Japan

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Publications (30)63.79 Total impact

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    ABSTRACT: In Japan, 12 viruses have been identified as causal agents of virus diseases of potato: Alfalfa mosaic virus, Cucumber mosaic virus, Potato aucuba mosaic virus, Potato leafroll virus (PLRV), Potato mop-top virus (PMTV), Potato virus A (PVA), Potato virus M (PVM), Potato virus S (PVS), Potato virus X (PVX), Potato virus Y (PVY), Tomato ringspot virus (ToRSV), and Tomato spotted wilt virus (TSWV). In a preliminary survey for viruses using ELISA, we found numerous virus species in landrace potatoes but only a few, e.g., PVY, in commercial ware potato crops. We have now modified a reverse transcription–polymerase chain reaction–microplate hybridization technique, developed for PVY, PLRV, and PMTV, to assay for the 12 viruses referred to above simultaneously. In leaf samples from 35 landraces, PVM, PVS, PVY, PLRV, PVA, and PVX were detected in 94%, 91%, 80%, 77%, 57%, and 43% of the landraces, respectively. These results indicate that potato landraces can act as a reservoir for these viruses with some landraces being asymptomatic. It is thus important to inspect and test for these viruses in seed potato production.
    No preview · Article · Jun 2013 · Potato Research
  • Tetsuji Ogawa · Akio Nakagawa · Tatsuji Hataya · Kazusato Ohshima
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    ABSTRACT: The genetic structure of Potato virus Y (PVY) populations in Japan was analysed using 20 isolates; five were retrieved from the public DNA sequence databases, and an additional 15 complete genomic sequences were determined using field samples collected in Japan. Recombination and phylogenetic analyses of a total of 149 isolates from Japan and other countries showed that PVY has three major lineages (C, N and O); at least one, two and six sublineages in C, N and O lineages, respectively. One recombination pattern was newly found among Japanese PVYNTN strain isolates, which was most closely related to the PVYNTN strain isolates previously found in Europe and North America. On the other hand, PVYO was a complex of several divergent lineages, and there were at least three non-recombinant subpopulations in Japan. Studies on nucleotide diversities of populations and phylogenetic relationships of the isolates in the PVY sequences showed that Japanese PVY populations were in part distinct from the European and North American populations.
    No preview · Article · Dec 2012 · Journal of Phytopathology
  • Tetsuo MAOKA · Syunpei Sugiyama · Yukio Maruta · Tatsuji Hataya
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    ABSTRACT: A complementary DNA (cDNA) macroarray was developed for simultaneous detection of 12 different potato viruses. A suitable region in the viral genome for each was selected for Alfalfa mosaic virus, Cucumber mosaic virus, Potato aucuba mosaic virus, Potato leafroll virus, Potato mop-top virus, Potato virus A, Potato virus M, Potato virus S, Potato virus X, Potato virus Y, Tomato ringspot virus, and Tomato spotted wilt virus, and their respective cDNAs were cloned into plasmid vectors. Capture probes for each virus ranging from 290 to 577 bp were generated by polymerase chain reaction (PCR) and immobilized on a nylon membrane. Total RNAs were extracted from each of these virus infected-plants, and cDNAs were synthesized from the RNA extracts using a random 9-mer primer. Subsequently, PCR reactions were performed using one primer pair for each of the 12 viruses. During PCR, amplified cDNAs were labeled with biotin and used as a target for hybridization analyses on a macroarray membrane. Hybridization signals between capture probes for the 12 viruses and their respective target cDNAs were observed using chemiluminescent or colorimetric detection. In all viruses, hybridization signals with capture probes were detected only when homologous virus targets were examined, and no hybridization to healthy plant extract was observed, facilitating identification of each virus. The results by colorimetric detection agreed with those obtained using chemiluminescence. The macroarray method developed was 5 × 102 to 4 × 106 times more sensitive than enzyme-linked immunosorbent assay and 5 to 5 × 104 times more sensitive than reverse-transcription PCR, except for Alfalfa mosaic virus. Colorimetric detection and substantial reduction in cross-hybridization signals much improved the method compared with other array-based detection methods for practical use.
    No preview · Article · Oct 2010 · Plant Disease
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    ABSTRACT: Spraing (brown rings or arcs) of potato caused by Potato mop-top virus (PMTV) occurred in a field of Tokachi, Hokkaido, Japan in 2005. To monitor the expansion of spraing-affected areas, we developed a soil diagnostic method that consisted of a bioassay using tomato as bait plant to trap the vector of PMTV, Spongospora subterranea, the causal agent of powdery scab of potatoes, followed by reverse transcription-polymerase chain reaction-microplate hybridization (RT-PCR-MPH) to detect the virus from roots of bait plants. After incubation of tomato seedlings grown with their roots immersed in a soil suspension at 18°C for 9 days, total RNA extracted from bait roots was analyzed by RT-PCR-MPH using PMTV-specific primers and a digoxigenin (DIG)-labeled probe. Soil diagnosis using the present method in an infested area revealed 137 of 224 fields (61.2%) were infested by PMTV although tubers harvested from only one of these fields had spraing.
    Full-text · Article · Apr 2010 · American Journal of Potato Research
  • Tatsuji Hataya
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    ABSTRACT: An accurate and reliable system to detect Potato spindle tuber viroid (PSTVd) was developed based on duplex reverse transcription-polymerase chain reaction (RT-PCR) in combination with a plant mRNA internal control to evaluate the quality of RNA extracted from a dormant potato tuber and the effectiveness of the RT-PCR. In addition, a non-infectious RNA based on the PSTVd sequence was generated in vitro as a readily available positive control without the possibility of escape; this RNA was distinguishable from true positives in PSTVd infection. This detection system will be useful in plant quarantine and phytosanitary certification applications.
    No preview · Article · Jun 2009 · Journal of General Plant Pathology
  • Tetsuo Maoka · Tatsuji Hataya
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    ABSTRACT: ABSTRACT The complete nucleotide sequence of the genome of Papaya leaf distortion mosaic virus (PLDMV) was determined. The viral RNA genome of strain LDM (leaf distortion mosaic) comprised 10,153 nucleotides, excluding the poly(A) tail, and contained one long open reading frame encoding a polyprotein of 3,269 amino acids (molecular weight 373,347). The polyprotein contained nine putative proteolytic cleavage sites and some motifs conserved in other potyviral polyproteins with 44 to 50% identities, indicating that PLDMV is a distinct species in the genus Potyvirus. Like the W biotype of Papaya ringspot virus (PRSV), the non-papaya-infecting biotype of PLDMV (PLDMV-C) was found in plants of the family Cucurbitaceae. The coat protein (CP) sequence of PLDMV-C in naturally infected-Trichosanthes bracteata was compared with those of three strains of the P biotype (PLDMV-P), LDM and two additional strains M (mosaic) and YM (yellow mosaic), which are biologically different from each other. The CP sequences of three strains of PLDMV-P share high identities of 95 to 97%, while they share lower identities of 88 to 89% with that of PLDMV-C. Significant changes in hydrophobicity and a deletion of two amino acids at the N-terminal region of the CP of PLDMV-C were observed. The finding of two biotypes of PLDMV implies the possibility that the papaya-infecting biotype evolved from the cucurbitaceae-infecting potyvirus, as has been previously suggested for PRSV. In addition, a similar evolutionary event acquiring infectivity to papaya may arise frequently in viruses in the family Cucurbitaceae.
    No preview · Article · Mar 2005 · Phytopathology
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    ABSTRACT: ABSTRACT Sequential polyacrylamide gel electrophoresis analyses showed many viroid-like RNAs in samples collected from citrus trees in Japan. Reverse transcription polymerase chain reaction and sequencing analyses of the amplified fragments verified that they were derived from variants of six citrus viroids, Citrus exocortis viroid (CEVd), Citrus bent leaf viroid (CBLVd) including CVd-I-LSS (a distinct variant of CBLVd), Hop stunt viroid, Citrus viroid III, Citrus viroid IV, and Citrus viroid OS. The samples induced symptoms with variable severity in Arizona 861-S1 'Etrog' citrons (Citrus medica L.) likely due to the varying accumulation patterns produced by the different viroids. Some of the symptoms caused by the samples harboring the citrus viroids other than CEVd were as severe as those caused by CEVd. Some source citrus trees showing the severe bark scaling characteristic of exocortis disease in trifoliate orange (Poncirus trifoliata (L.) Raf.) rootstocks contained only citrus viroids other than CEVd in complex. This indicates that certain exocortis-like diseases in Japan were caused by some combination of citrus viroids not including CEVd.
    Full-text · Article · Jun 2002 · Phytopathology

  • No preview · Article · Jan 2002
  • T Hataya · R Arimoto · N Suda · I Uyeda
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    ABSTRACT: The 3'-terminal sequence of hop mosaic virus (HpMV) genomic RNA was determined. A cDNA of approximately 1.8 kbp was amplified from the HpMV genome by 3' RACE using a degenerate primer, which was designed to anneal to the overlapping region of open reading frames (ORFs) 2 and 3 of eight carlavirus genomes. The sequence contained three ORFs, encoding proteins of 7-, 34-, and 11-kDa, which corresponded to ORFs 4, 5, and 6 of the carlavirus genome, respectively. The amino acid sequence of ORF 5, encoding the coat protein (CP) of HpMV, shows the highest identity (67%) to that of Hop latent virus (HpLV). The HpMV CP N-terminal sequence differs from that of HpLV, but the central and C-terminal sequences of the CP of both viruses are similar. The sequence similarity possibly causes the cross-reaction of heterologous antibodies of HpMV and HpLV. Phylogenetic analyses based on the CP amino acid and 3' non-coding region sequences indicate close relationships among HpMV, HpLV, and Potato virus M. We report here the first molecular characterization of HpMV genomic RNA.
    No preview · Article · Nov 2001 · Archives of Virology
  • T Hataya · K Uchino · R Arimoto · N Suda · T Sano · E Shikata · I Uyeda
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    ABSTRACT: The complete nucleotide sequence of the hop latent virus (HpLV) genome was determined. The viral RNA genome is 8,612 nucleotides long, excluding the poly(A) tail, and contains six open reading frames (ORFs), which encode putative proteins of 224-kDa (ORF 1), 25-kDa (ORF 2), 11-kDa (ORF 3), 7-kDa (ORF 4), 34-kDa (ORF 5), and 12-kDa (ORF 6). ORF 5 encodes the coat protein as demonstrated by N-terminal sequencing of three proteolytic peptides derived from the virus particle. The genome organization of HpLV is similar to that of other species in the genus Carlavirus, and the overall sequence of HpLV is more similar to that of Potato virus M than to sequences of other carlaviruses reported to date. The amino acid sequences of the putative methyltransferase, RNA helicase, and RNA-dependent RNA polymerase encoded in ORF 1 and an 'accessory' helicase encoded in ORF 2 of the HpLV genome were compared with those of viruses in the 'tymo' lineage: the genera Carlavirus, Potexvirus, Allexivirus, Foveavirus, Trichovirus, Capillovirus, Vitivirus, and Tymovirus. The phylogenetic relationships among the viruses in these genera are discussed. This is the first molecular characterization of a carlavirus infecting hop plants.
    No preview · Article · Feb 2000 · Archives of Virology
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    ABSTRACT: The sensitivity of gene diagnostic methods for detecting chrysanthemum stunt viroid (CSVd) in 2 M LiCl‐soluble nucleic acids extracted from chrysanthemum plants was examined. Dot blot hybridization using DIG‐labeled cRNA and cDNA probes was compared. The cRNA probe was at least 25 times more sensitive than the cDNA probe and had a superior signal‐to‐noise ratio. Although reverse transcription and polymerase chain reaction (RT‐PCR) is much more sensitive in theory, in practice it was only 5 times more sensitive than hybridization using the cRNA probe. In addition, the nucleic acid extracts used inhibited the cDNA amplifications, probably due to natural inhibitors such as polysaccharides and polyphenolic compounds in the plants. HC1 treatment following ethanol precipitation of the nucleic acid extracts was effective in rapidly eliminating the inhibitory factors. The combination of RT‐PCR and hybridization on microplate well expanded the detectable range and was at least 25 times more sensitive than agarose gel analysis of the RT‐PCR products. Based on these results, the use of practical diagnosis for protecting chrysanthemum from CSVd and for certifying CSVd‐free chrysanthemum is discussed.
    No preview · Article · Aug 1999 · Archives of Phytopathology and Plant Protection
  • Kenji Nakahara · Tatsuji Hataya · Ichiro Uyeda
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    ABSTRACT: A simple, rapid method of nucleic acid extraction on a microcentrifuge tube scale for detecting viroids is presented. Five distinct citrus viroids (CVds), chrysanthemum stunt viroid (CSVd), hop stunt viroid (HSVd), hop latent viroid (HLVd) and potato spindle tuber viroid (PSTVd) were detected in their natural host plants by hybridization using cRNA probes and reverse transcription-polymerase chain reaction (RT-PCR). Nucleic acids (NA) were liberated from tissues by incubation in a buffer containing potassium ethyl xanthogenate (PEX) without tissue homogenization, and then precipitated with ethanol (NA-PEX). All the viroids except CVd-IV could be detected clearly in NA-PEX by hybridization. HSVd, HLVd and PSTVd could also be detected in NA-PEX by RT-PCR. Although CVds and CSVd could not be detected in NA-PEX by RT-PCR, they were detected after further purification: differential precipitation with 2-butoxyethanol and HCl treatment followed by ethanol-precipitation. In addition, PCR in the presence of tetramethylammonium chloride specifically amplified the cDNA of all five distinct CVds under the same temperature and cycle conditions. Since all the viroids could be detected in NA liberated by PEX, the amount of NA extracted by the method described here is sufficient for detecting viroids, enabling the processing of a large number of samples.
    No preview · Article · Feb 1999 · Journal of Virological Methods
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    Full-text · Article · Jan 1999
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    ABSTRACT: Five kinds of synthetic oligonucleotide probes labeled with biotin (BIO) were designed for the detection of potato spindle tuber viroid (PSTVd), and their sensitivities were compared with that of a digoxigenin (DIG)- or BIO-labeled cDNA probe. Although each oligonucleotide probe alone was less sensitive than the DIG-cDNA probe, sensitivity was increased by using a mixture of two or all of the five oligonucleotide probes. The sensitivity of a PSmix1-5 probe, which was a mixture of five oligonucleotides, was the same as that of a DIG-labeled cDNA probe, which can detect 7.8 pg of purified PSTVd and PSTVd in nucleic acids, equivalent to extracts from 20 microg of infected potato leaf and 310 microg of infected potato tuber. Using the PSmix1-5 probe, PSTVd in all leaves and tubers of seven potato cultivars could be detected without any background. Moreover, with the PSmix1-5 probe, the hybridization time could be shortened to 2 h without any decrease in sensitivity, whereas the sensitivity of the cDNA probes clearly decreased when the hybridization time was shortened. Hybridization using a mixture of several oligonucleotide probes may be applicable to the gene diagnosis of other viroids and viruses.
    No preview · Article · May 1998 · Journal of Virological Methods
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    Kenji Nakahara · Tatsuji Hataya · Ichiro Uyeda
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    ABSTRACT: Nucleic acid sequence-based amplification (NASBA) according to the standard protocol failed to amplify cRNA of viroids, probably because of their GC-rich and intramolecular base-paired structure. However, NASBA in the presence of inosine 5′-triphosphate successfully amplified the cRNAs to viroids in total nucleic acid extracts from citrus plants. As sequence specificity of the cRNA to viroids was confirmed by northern analysis, the amplification and fidelity of cRNAs are sufficient for the sensitive and specific detection of viroids.
    Full-text · Article · Apr 1998 · Nucleic Acids Research
  • T Hataya · K Nakahara · T Ohara · H Ieki · T Kano
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    ABSTRACT: Nucleotide sequences of group I citrus viroids Ia (CVd-Ia) and citrus bent leaf viroid (CBLVd, formerly designated CVd-Ib) isolated from citrus plants in Japan, the Philippines and China have been determined. Citrus samples in Japan and the Philippines contained CVd-Ia, which consists of 328 nucleotides(nt). Although 10 nt longer than the type CBLVd-225A in Israel they share 94% identity in overall nucleotide sequence. The Philippines sample also contained a 329-nt long CVd-Ia sequence variant, in which one base insertion and three substitutions were observed. A citrus in China contained CBLVd, which consists of 318 nt and shares 98% identity to CBLVd-225A. CVd-Ia was clearly separated from CBLVd by two 5-nt insertions located in upper (5'-AGCUG-3') and the lower (5'-CUUCU-3') strand of the right terminal region (which is also designated T2 domain) in rod-like secondary structure. Since both of the additional 5-nt sequences are similar to the adjacent sequences (5'-AGUUG-3' and 5'-CUUCU-3'), we hypothesize that CVd-Ia is a derivative of CBLVd caused by partial sequence duplications and substitutions taking place in the right terminal region.
    No preview · Article · Feb 1998 · Archives of Virology
  • Kenji Nakahara · Tatsuji Hataya · Ichiro Uyeda · Hiroyuki Ieki

    No preview · Article · Jan 1998 · Japanese Journal of Phytopathology
  • Masamichi Isogai · Ichiro Uyeda · Tatsuji Hataya
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    ABSTRACT: A strategy was designed to efficiently clone double-stranded RNAs (dsRNAs) of unknown sequences and very low availability. From rice plants infected with rice black streaked dwarf fijivirus (RBSDV), ten dsRNA genomic segments were extracted directly. The 3' ends of the plus and minus strands of the dsRNAs were polyadenylated and then used as templates for an initial reverse transcription using an oligo-dT-containing adapter primer (AP). The first-strand cDNAs of both polarities were annealed, filled in and amplified by the polymerase chain reaction using one primer containing an adapter region sequence identical to that in the AP. The amplified cDNA products corresponded in size to the full lengths of RBSDV S5, S6, S7, S8, S9 and S10; full length cDNA clones to RBSDV S8, S9 and S10 containing both terminal nucleotide sequences were obtained. Moreover, the nucleotide sequences of six full length cDNA clones were the same as those previously reported. These data indicate that this method may be applicable to full length cDNA cloning of dsRNAs.
    No preview · Article · Jan 1998 · Japanese Journal of Phytopathology
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    Full-text · Article · Jan 1997
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    I Uyeda · H Suga · S Y Lee · J Yan · T Hataya · I Kimura · E Shikata
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    ABSTRACT: The complete nucleotide sequence of rice ragged stunt virus genome segment 9 (S9) was determined. The S9 segment is 1132 nucleotides long and has a long open reading frame starting from the first AUG codon at nucleotide position 14-16 and terminating at a UAG codon located at 1028-1030, which could encode a polypeptide with an Mr of 38 600 (P9). The encoded polypeptide has no sequence homology to polypeptides of any other plant reoviruses published previously. An immunological study demonstrated that P9 was the smallest of the structural proteins. The P9 polypeptide was expressed as a fusion protein with maltose binding protein in Escherichia coli. Antisera to purified virions and to the fusion protein reacted with both the bacterially expressed polypeptide and the smallest polypeptide of the purified virus in immunoblotting analyses.
    Preview · Article · May 1995 · Journal of General Virology

Publication Stats

435 Citations
63.79 Total Impact Points


  • 1989-2013
    • Hokkaido University
      • • Laboratory of Pathogen-Plant Interactions
      • • Research Faculty of Agriculture
      • • Department of Agrobiology and Bioresources
      Sapporo, Hokkaidō, Japan
  • 1991
    • Nippon Paint Co., Ltd.
      Ōsaka, Ōsaka, Japan