Bao-Yun Li

China Agricultural University, Peping, Beijing, China

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Publications (7)5.07 Total impact

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    ABSTRACT: Drought stress is one of the major factors affecting in wheat yield and grain quality. In order to investigate how drought stress might influence wheat quality during grain filling, three wheat cultivars Gaocheng 8901, Jagger and Nongda 3406 were subjected to drought stress during the grain filling stage. Neither globulin and glutenin, nor the relative percentage of amylose significantly changed following drought treatments, whereas albumin and gliadin concentrations did. The SDS-sedimentation, which has a strong linear correlation with wheat baking quality was markedly decreased following drought stress. These results indicated that drought had an adverse effect on wheat quality. In order to investigate the protein complexes in the wheat flour, the data from native PAGE and SDS-PAGE were combined and a total of 14 spots were successfully identified, and of these eight protein types were determined to be potential complex forming proteins.
    No preview · Article · May 2014 · Journal of Integrative Agriculture
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    ABSTRACT: Complete genomic and cDNA sequences of the Waxy gene encoding granule-bound starch synthase I (GBSSI) were isolated from the rye genome and characterized. The full-length rye Waxy genomic DNA and cDNA are 2767 bp and 1815 bp, respectively. The genomic sequence has 11 exons interrupted by 10 introns. The rye Waxy gene is GC-rich, with a higher GC frequency in the coding region, especially in the third position of the codons. Exon regions of the rye Waxy gene are more conserved than intron regions when compared with the homologous sequences of other cereals. The mature rye GBSSI proteins share more than 95% sequence identity with their homologs in wheat and barley. A phylogenetic tree based on sequence comparisons of available plant GBSSI proteins shows the evolutionary relationship among Waxy genes from rye and other plant genomes. The identification of the rye Waxy gene will enable the manipulation of starch metabolism in rye and triticale.
    No preview · Article · Jul 2009 · Genome
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    ABSTRACT: Complete genomic and cDNA sequences of the Waxy gene encoding granule-bound starch synthase I (GBSSI) were isolated from the rye genome and characterized. The full-length rye Waxy genomic DNA and cDNA are 2767 bp and 1815 bp, respectively. The genomic sequence has 11 exons interrupted by 10 introns. The rye Waxy gene is GC-rich, with a higher GC frequency in the coding region, especially in the third position of the codons. Exon regions of the rye Waxy gene are more conserved than intron regions when compared with the homologous sequences of other cereals. The mature rye GBSSI proteins share more than 95% sequence identity with their homologs in wheat and barley. A phylogenetic tree based on sequence comparisons of available plant GBSSI proteins shows the evolutionary relationship among Waxy genes from rye and other plant genomes. The identification of the rye Waxy gene will enable the manipulation of starch metabolism in rye and triticale.Des séquences génomiques et ADNc complètes du gène Waxy, lequel code pour l'amidon synthétase liée au grain I (GBSSI), ont été clonées et caractérisées chez le seigle. Les clones génomiques et ADNc du gène Waxy mesurent respectivement 2767 pb et 1815 pb. La séquence génomique compte 11 exons interrompus par 10 introns. Le gène Waxy du seigle est riche en GC et affiche une fréquence plus élevée en GC dans la région codante, particulièrement à l'endroit de la troisième position des codons. Les exons du gène Waxy du seigle sont plus conservés que les introns lorsque ces séquences sont comparées à leurs homologues chez d'autres céréales. Les protéines GBSSI matures présentent plus de 95 % d'identité avec leurs homologues chez le blé et l'orge. Un arbre phylogénétique comprenant les séquences de toutes les protéines GBSSI connues chez les plantes montre les relations évolutives entre les gènes Waxy du seigle et des autres plantes. L'identification du gène Waxy chez le seigle rendra possible la manipulation du métabolisme de l'amidon chez le seigle et le triticale.
    No preview · Article · Jun 2009 · Genome
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    ABSTRACT: Polyphenol oxidase (PPO) is often regarded as a major factor resulting in time-dependent darkening and discoloration of Asian noodles and other wheat end products. To understand the relationship between variation of PPO genes and PPO activity of seed, the three PPO genes, which express in immature wheat grain, were investigated in 216 common wheat cultivars. The results indicated that only TaPPO-A1 and TaPPO-D1 showed high polymorphisms related to PPO activity. Two alleles in both TaPPO-A1 (TaPPO-A1a and TaPPO-A1b) and TaPPO-D1 (TaPPO-D1a and TaPPO-D1b) were detected using denaturing polyacrylamide gel electrophoresis. Wheat cultivars with TaPPO-A1b usually showed higher PPO activity than those with TaPPO-A1a. The TaPPO-D1a allele was often found in lower-PPO-activity cultivars, compared with TaPPO-D1b. The sequencing results of DNA fragments confirmed that two introns existed in TaPPO-D1 like TaPPO-A1. Some variation of introns was detected in the two alleles of TaPPO-D1. During seed development, the high-PPO-activity cultivar, Yangmai 158 (TaPPO-A1b/TaPPO-D1b) showed higher transcription of the two PPO genes, in comparison to low-activity cultivar, Yongchuanbaimai (TaPPO-A1a/TaPPO-D1a). These results suggested that variation in introns may influence the transcription of TaPPO-A1 and TaPPO-D1 in immature seeds of wheat.
    No preview · Article · Feb 2007 · Euphytica
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    ABSTRACT: Fourteen wheat cultivars were identified into six types of Wx proteins combinations using 6% SDS-PAGE. PCR primers were designed according to the three Wx genes sequences and their mutants, respectively. A 327 bp-band was amplified from the Wx-A1 mutant,while the band was absent for the normal alleles at the Wx-A1 locus,as well as the presence or absence of a 187 bp PCR fragment at the Wx-B1 locus and a 700 bp PCR fragment at the Wx-D1 locus, respectively, corresponding to the normal and mutant alleles. Compared with the former studies, shorter and more different PCR products at three loci, amplified by the primers designed for Wx-B1 gene can be separated in 2% agarose gel, which enables screening breeding lines for noodle use faster and effectively.
    Preview · Article · Feb 2004 · Acta Genetica Sinica
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    ABSTRACT: Random amplified polymorphic DNA (RAPD) analysis was performed on common wheat Chinese Spring, H. villosa, addition lines of H. villosa chromosome in CS, substitution line 3V of H. villosa chromosome in Triticum aestivum. A genome specific polymorphic DNA segment from H. villosa, OPF02757, was obtained. On the basis of cloning and sequencing of OPF02757, two PCR primers were designed and a genome specific PCR marker for H. villosa was established. The PCR marker including 677 bp was localized on all the seven pairs of H. villosa chromosomes. The result of PCR amplification by the primers indicated that there was a specific band of 677 bp in the materials containing H. villosa Chromosome such as T. aestivum-H. villosa addition, T. aestivum-H. villosa substitution, T. aestivum-H. villosa amphidiploid, T. durum-H. villosa amphidiploid and H. villosum from different accessions, and there was no specific band of 677 bp if the materials did not contain H. villosa chromosome, such as T. aestivum, T. durum, Secale cereale, Hordeum vulgare, Thinopyrum elongatum, Thinopyrum intermedium. Therefore, the PCR maker of 677 bp is specific to H. villosa genome, and could be used as molecular marker for detection of chromosomes of H. villosa in wheat.
    Preview · Article · May 2003 · Acta Genetica Sinica
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    ABSTRACT: Random amplified polymorphic DNA (RAPD) analysis was performed on common wheat of Chinese Spring, addition lines of H. villosa chromosome in CS and H. villosum from different accessions with 100 random 10-base primers. A chromosome-specific polymorphic DNA segment for H. villosa, OPF02(750), was obtained from all addition lines of H. villosa chromosome in CS and H. villosum which belong to different accessions. The result amplified by primer OPF02 of all addition lines of H. villosa chromosome in CS indicated that all the seven pairs of H. villosa chromosomes contain OPF02(750) segment. There was no OPF02(750) in all Triticum aestivum and T. durum tested. Using OPF02, We confirmed that NAU302, an addition line of H. villosa chromosome 3V, had lost its chromosome 3V of H. villosa. Therefore, OPF02(750) is specific to chromosomes of H. villosa, and could be used as a molecular marker for detection of chromosome of H. villosa in wheat.
    No preview · Article · Jun 2002 · Acta Genetica Sinica

Publication Stats

35 Citations
5.07 Total Impact Points

Institutions

  • 2003-2014
    • China Agricultural University
      • • College of Agronomy and Biotechnology
      • • Department of Plant Breeding and Genetics
      Peping, Beijing, China