[Show abstract][Hide abstract] ABSTRACT: Helicobacter pylori causes various diseases such as chronic gastritis, peptic ulcer and gastric cancer. While majority of the people infected with H. pylori is asymptomatic, 15-20 % of them develop such diseases. The main factors, which determine the development of H. pylori related diseases might be bacterial virulence, host genetic and environmental factors.The aim of this study was to reveal the factors that play a role in the disease development in patients with reflux esophagitis and peptic ulcer, infected with Helicobacter pylori. Environmental factors such as medical agents, smoking and body mass index were evaluated. The factors specific to bacteria such as vacA, CagA, babA and iceA virulence genotypes and the host factors such as IL-1, IL-2, IL-4, IL-6, IL-10, IL-12, interferon-γ, TNF-α, ve TGF-β1 gene polymorphisms were compared between the two groups.H. pylori infected twenty five patients with reflux esophagitis and peptic ulcer were enrolled in the study. There was no statistical difference between the two groups regarding environmental factors. IL-2 -330T +166T (p=0.037) and IL10 -1082A; -819C (p=0.049) gene polymorphisms were significantly more common in the group of patients with peptic ulcer compared to the group with reflux esophagitis. In both groups of patients, either with reflux esophagitis or peptic ulcer, multiple H. pylori virulence genotypes (cagA, vacA, babA) (mean values 74 %, 78 %, 54 % respectively) were observed.In this study, we revealed that cytokine gene polymorphisms may play a role in the development peptic ulcer while H. pylori virulence genotypes seem to be crucial for the development of associated diseases (Tab. 4, Ref. 51). Keywords: Helicobacter pylori genotypes, single nucleotide polymorphisms, reflux oesophagitis, peptic ulcer.
Preview · Article · May 2014 · Bratislavske lekarske listy
[Show abstract][Hide abstract] ABSTRACT: The rapid, simple and non-invasive diagnosis of Helicobacter pylori (H. pylori) infection is important in implementing chemotherapy in appropriate manner, and in assessing persistent H. pylori infection after eradication therapy. The ImmunoCard STAT! HpSA kit (Meridian Bioscience, Europe) is a lateral flow chromatography test which utilizes a monoclonal anti-H. pylori antibody. In this study, we investigated the usefulness of the ImmunoCard STAT! HpSA test before and after eradication therapy on patients referred to undergo upper gastrointestinal endoscopy.
Sixty-five consecutive patients who were referred to undergo upper gastrointestinal endoscopy at the Department of Gastroenterology, Karadeniz Technical University Medical School, Turkey between February and August 2005 were included in this study. The ImmunoCard STAT! HpSA was compared with 4 invasive tests (histology, gram staining, rapid urease test, and culture). The reference method was defined as positive when 2 of the 4 invasive tests were positive. A negative H. pylori status was considered when all 4 tests present concordant negative results.
Overall, the ImmunoCard STAT! HpSA test had 77.8% sensitivity, 79.3% specificity, 82.4% positive predictive value (PPV) and 74.2% negative predictive value (NPV) in all patients. With regard to pre-treatment values, the sensitivity was 70.6%, specificity 70.6%, PPV 100% and NPV 100% while on post-treatment group the sensitivity was 84.2%, specificity 64.7%, PPV 72.7% and NPV 78.6%.
Our results indicate that the ImmunoCard STAT! HpSA test is a rapid, simple, and helpful procedure not only to determine H. pylori infection but also to assess the success of eradication therapy.
Full-text · Article · Jul 2006 · Saudi medical journal
[Show abstract][Hide abstract] ABSTRACT: The Bernard-Soulier syndrome (BSS) is a rare disorder of platelet function which is characterized by a prolonged bleeding time, giant platelets, and thrombocytopenia. Treatment of hemorrhage in patients with BSS usually requires platelet transfusion, but alloimmunization frequently occurs after repeated transfusions. In such patients prevention of bleeding may be difficult. In the literature there are a few cases of patients with BSS suffering from recurrent bleedings and refractory to platelet transfusions who were treated successfully with factor VIIa. Here, we describe another BSS patient refractory to platelet transfusions who had presented with severe gastrointestinal bleeding and was treated successfully with recombinant factor VIIa.
No preview · Article · Mar 2005 · Chirurgische Gastroenterologie
[Show abstract][Hide abstract] ABSTRACT: The occurrence of cagA and vacA alleles among Helicobacter pylori isolates from Turkish patients and their relationship with ulcer disease outcome was investigated. Among isolates from 47 patients with peptic ulcer disease and 51 patients with non-ulcer dyspepsia, 72.3% and 44.4%, respectively, were cagA-positive (p 0.019). Most (88.8%) isolates were typed as vacA s1, and all of these were subtype s1a. The commonest (51.0%) vacA genotype was s1a m1. The results of multivariate analysis indicated that infection with cagA-positive H. pylori was the only variable associated with an increased risk of peptic ulcer disease (odds ratio, 3.01; 95% confidence interval, 1.27-7.10; p 0.012).
Full-text · Article · Jan 2005 · Clinical Microbiology and Infection
[Show abstract][Hide abstract] ABSTRACT: Apoptotic and anti-proliferative effects of heparin on a number of cancers have been described. There have been no studies analyzing the effect of heparin on human hepatoma cells. The aim of this study was to investigate the effect of heparin on human hepatoma cell line, HepG2.
HepG2 cell line was cultured with different concentrations of heparin. Colony count, viability assay, percentage of the apoptosis and proliferative index were assessed at the end of the 7th day. Trypan blue was used to assess viability. Apoptosis and proliferative indexes were assessed by flow-cytometry.
Hepatoma cells were arrested at the G0/G1 phase with heparin incubation and proliferative indexes decreased significantly in 20, 40 and 80 U/mL of heparin concentrations in comparison with the control (36 +/- 1%, 30 +/- 5% and 29 +/- 8% vs. 44 +/- 1%, p < 0.01). Flow cytometry revealed a statistically significant increase in apoptosis in groups incubated with 40 and 80 U/mL of heparin in comparison with the control (39 +/- 26% and 58 +/- 18% vs. 0.83 +/- 1.3%, p < 0.01). Colony counts per well and viable cells per microL decreased significantly in 80 U/mL of heparin.
Heparin leads to a significant anti-proliferative and an apoptotic effect on human hepatoma cells in vitro.
No preview · Article · Nov 2003 · Hepato-gastroenterology
[Show abstract][Hide abstract] ABSTRACT: In the present work, the effect of interferon-alfa-2a on human hepatoma HepG2 cells has been studied. HepG2 cells were cultured with different concentrations of IFN-α of for 7 days, and then apoptotic and proliferative indexes were evaluated. It has been shown that upon such incubation, hepatoma cells were arrested in G0/G1 phase; proliferative indexes were found to be decreased significantly and apoptosis rate was increased at IFN-α concentrations of 50 U/ml and 100 U/ml compared to the control (29% ± 4.5% and 27% ± 4.6% vs 44% ± 3.2%, p < 0.01; and 34% ± 9.6% and 43% ± 22% vs 0.8% ± 1.3%, p < 0.01 respectively). Colony counts per well decreased significantly at IFN-α concentrations of 100 U/ml compared to control. Clinical relevance of these finding remain to be established with further studies.
No preview · Article · Jun 2003 · Eksperimentalʹnai͡a onkologii͡a
[Show abstract][Hide abstract] ABSTRACT: Here we describe two women with polycythemia vera presented with fulminant hepatic failure due to acute Budd-Chiari syndrome. Both had a history of severe abdominal pain and distention of short duration. Clinical and laboratory findings showed fulminant hepatic failure due to acute Budd-Chiari syndrome. Diagnosis was confirmed with abdominal ultrasonography and Doppler ultrasonography showing ascites, hepatomegaly, portal hypertension and total occlusion of hepatic veins. Complete blood count and other clinical findings were compatible with polycythemia vera in both patients. Patients were treated successfully with early administration of continuous heparin infusion, repeated phlebotomies and hydroxyurea. We emphasize here early diagnosis and effective treatment in such fulminant cases may be life saving.
No preview · Article · Mar 2003 · Hepato-gastroenterology