Zdravko Petrinec

University of Zagreb, Zagrabia, Grad Zagreb, Croatia

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Publications (39)19.43 Total impact

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    ABSTRACT: The histopathological structure as well as content and distribution of mucosubstances in the mid-gut mucosa of the honeybee (Apis mellifera) treated with the phyto-pharmacological preparation Nozevit was studied. For the purpose of describing neutral, acid and sulphate mucopolysaccharides we used the Periodic Acid-Schiff Reaction (PAS), Alcian blue-specific (pH = 1.0 and 2.5) and Toluidine blue-specific staining. Based on our results we have concluded that the herbal preparation Nozevit induces the production and secretion of mucous from the epithelial layer of treated bees, and additionally coats the peritrophic membrane to form a firm and resilient envelope. Thus, the preparation may ensure protection from new invasion with Nosema sp. spores and also from normal physiological processes.
    No preview · Article · Jul 2011 · Veterinární medicína
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    ABSTRACT: With 9 figures and 2 tables Localization and activity levels of the following digestive enzymes in the intestine of free-living large-scaled gurnard were determined: non-specific esterase, alkaline and acid phosphatase as well as aminopeptidase. Enzymatic activity of the four enzymes was confirmed in all intestine parts but with different distribution through the enterocytes and varying in diverse intensity according to intestine part. This research is part of a broader research project on the biology of economically important fish from the Adriatic Sea. Our study reveals that in the large-scaled gurnard, the middle and posterior intestinal segments play the major role in digestion and absorption of proteins, whereas all parts of the intestine participate in lipid absorption and intracellular digestion. The high protein and lipid content in the diet of the large-scaled gurnard is most likely responsible for high activities of esterase, alkaline phosphatase and aminopeptidase, as they are involved in digestion and absorption of proteins and lipids.
    No preview · Article · Mar 2011 · Anantomia Histologia Embryologia
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    ABSTRACT: Previously, Nosema infections in European honey bees have been attributed solely to N. apis (Ellis and Munn, 2005), but it now appears that N. ceranae is an emerging pathogen that has increased its distribution (Klee et al., 2007). Nosema ceranae has not been detected in Croatia to date, but we have suspected its presence in pure or mixed infections with N. apis, because of a high percentage of Nosema infections being found during summer, and, because N. ceranae has been diagnosed in some neighbouring countries. The aim of this research was to determine the presence of N. ceranae, its prevalence and distribution in all 21 districts of Croatia, using a multiplex PCR. The diversity of Nosema species was studied using 204 honey bee samples originating mainly from bee colonies with different pathological problems like depopulation, weakness or high colony mortality. Samples of dead bees were collected during the first spring inspection of colonies, in February and March 2009. Each common sample represented one location or one apiary. Samples of honey bees were analyzed according to the OIE Manual (Anon, 2008). After light microscopy, a total of 150 spore samples from different localities were selected and investigated by multiplex PCR. Extraction of genomic DNA and further molecular analysis was performed as follows: for each of selected suspensions of isolated Nosema spores, an aliquot of 50 μl was transferred to a fresh tube, boiled at 100°C for 30 mins and centrifuged at 14,000 g for 10 mins. Thirty μl of supernatant was removed and supplemented with 10x TE buffer to a final concentration of 10mM Tris and 5mM EDTA, pH8. This supernatant served as source of template DNA and was stored at -20°C, or, used immediately for multiplex PCR (Higes et al., 2006). Primers were selected, taking into account that primer sequences were specific to each of the two species, and that both amplicons could be simultaneously amplified (Anon, 2008) and separated using agarose gel electrophoresis for visualization of results. The molecular size of PCR products were determined by electrophoresis in a 2% agarose TAE (Tris-acetate-ethylene diamine tetra-acetic acid) gel in standard TAE buffer, stained with SYBR green, and visualized using UviTec gel documentation system.
    Full-text · Article · Aug 2010 · Journal of Apicultural Research
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    ABSTRACT: In this paper, pathological changes found at the necropsy of a Red-eared slider (Trachemys scripta elegans) are described. The turtle was delivered to the department by the owner with suspicion of a progressed shell disease. External examination showed hemorrhagic spots especially on the head, parts of the abdominal shell, and on the leg and shell junctions. Also characteristic crater like lesions of the plastron and carapace were identified. After the removal of the abdominal part of the shell, three irregular tissue neoplasms of hard elastic consistency on the inner side of the shell and pathological changes on other internal organs were noticed. With a special histological staining (Sudan HI), lipid vacuoles in the hepatocytes and renal glomeruli were determined - an evidence of fatty infiltration and degeneration. The pet turtle died of an of an ulcerative disease of the shell. Macroscopical and histopathological findings are discussed.
    No preview · Article · Jul 2010
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    I.T. Tlak Gajger · O Vugrek · D Grilec · Z Petrinec
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    ABSTRACT: Nosema disease of European honey bees afflicts bees worldwide. Nosema ceranae is a recently described microsporidian parasite of the honey bee (Apis mellifera) and its geographical distribution is not well known. The disease may have many negative effects on bee colonies and cause high losses for apiculture and consequently in agriculture. With this in mind, a total of 204 samples of dead bees from different localities in Croatia were selected and investigated for distribution, prevalence and diversity of N. ceranae infection, using light microscopic examination and multiplex PCR. Our results show that N. ceranae is the only nosema species found to infect honeybees in our geographically varied collection. The nucleotide sequences of amplicons from Nosema-infested honeybee samples were 100% identical with the N. ceranae sequence deposited in the GenBank database. N. ceranae infected bees were found in samples collected from each of 21 districts, and in all three climatic areas, i.e., mediterranean, mountain, and continental parts regions of Croatia.
    Full-text · Article · Jan 2010 · Veterinární medicína
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    Ivana Tlak Gajger · Oliver Vugrek · Ljiljana Pinter · Zdravko Petrinec
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    ABSTRACT: Nosema disease affects adult honey bees and due to its mostly incon-spicuous signs and the need for eradication by exchange of frames with brood from a disinfected hive and often use of new wax, beekeepers de-vote insufficient attention or often neglect the disease. Also, there is a problem of controlling nosemosis, especially caused with N. ceranae be-cause of its asymptomatic duration and prohibition of using antibiotics in the treatment of apian diseases in the European Union, as well as in Croatian regulations. We have predicted great results for use of protein pollen patties with "Nozevit" herbal preparation, as a feed supplement for bee colonies, where it can have an effect on brood rearing (colony strength) and at the same time reduce the number of Nosema ceranae spores. The aim of this study was to assess the effectiveness of the "Noze-vit" phyto-pharmacological preparation in protein/pollen substitute pat-ties for treatment of nosema disease in comparison with patties without "Nozevit" and sugar solution in a similar control group.
    Full-text · Article · Dec 2009 · American Bee Journal
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    ABSTRACT: For years scientists have suspected that the environment plays a role in neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and multiple sclerosis. Mycotoxin fumonisin B1 (FB1) is produced by several Fusarium species, mainly by Fusarium verticilioides, which is one of the most common fungi associated with corn worldwide. Fumonisins are known to cause equine leukoencephalomalacia, a disease associated with the consumption of corn-based feeds contaminated with FB1. Here we have reported chronic experimental toxicosis in one-year-old carp (Cyprinus carpio L.) receiving feed containing 100 mg kg-1 or 10 mg kg-1 of added FB1 for 42 days. We focused on fumonisin toxicity in the fish brain. After staining with hemalaun-eosin, histology of the fish brain revealed vacuolated, degenerate, or necrotic neural cells, scattered around damaged blood capillaries and in the periventricular area. These findings suggest that fumonisin, although it is a hydrophilic molecule, permeated the blood-brain barrier of young carp and had a toxic effect on neuronal cells.
    Full-text · Article · Dec 2009 · Archives of Industrial Hygiene and Toxicology
  • S Kuzir · Z Kozarić · E Gjurcević · B Bazdarić · Z Petrinec
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    ABSTRACT: Garfish, Belone belone (Linnaeus, 1761) is an elongate, slander fish inhabiting the Eastern Atlantic and Mediterranean Sea. These fish from the Belonidae (Actinopterygii) family have important commercial value for Croatian fisheries. Samples for research were collected from experimental hatching in the Novigrad Sea. Preparation techniques included fixation in buffered formalin, trypsin clearing and staining with alcian blue and alizarin red. As little is known of garfish osteology and bone morphology, the main goal of this study was to describe ossification process in garfish fry. At hatching, no skeletal structure is present. Newly-hatched larvae also had no osteological elements. Ossification started at 7 day post-hatching (DPH) [total length (TL) 18 mm] with head bones and vertebral neural arch. Head skeleton continued to develop mostly over the period from 7 to 10 DPH. At 21 DPH (TL 49 mm), ossification process seemed to be finished, but it was not possible to distinguish borders of all bones. The primary interest of our research was to understand the growth dynamics as well as transformation of supporting body elements from cartilage to bone. At the end, developmental characteristics and functional aspects of this formation in different fish species are discussed.
    No preview · Article · Sep 2009 · Anantomia Histologia Embryologia
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    ABSTRACT: Nosema disease is a microsporidian Nosema sp. parasitic disease of adult bees. The disease is spread worldwide, and it causes significant losses to apiculture and economy in general. EU and Croatian legal regulations prohibit the use of antibiotics in the treatment of bee diseases, due to possible development of resistance to used chemotherapeutic agents, masking of disease, possible relapses, as well as harmful antibiotic residues or their secondary metabolites in bee products. Therefore, the production and use of natural phyto-pharmacological preparations in the treatment of Nosema disease is a necessity. The aim of this research was to test the performance of the herbal preparation "Nozevit" as a preventive measure against artificial infection with N. apis spores, and it's curative effect in the treatment of bees affected by Nosema disease.
    No preview · Article · May 2009 · American Bee Journal

  • No preview · Article · Jan 2009
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    ABSTRACT: Twenty seven farmed Bluefin tunas (Thunnus thynnus) died in the Adriatic Sea (Croatia) in farming cages, in period of three days. After delivering two died tunas on laboratory examination at Department for Biology and Pathology of Fish and Bees, of Veterinary Faculty in Zagreb, dissection was carried out. Macroscopically, the most prominent structural change was found in liver which was effusive in size, extremely yellowish and with fragile coherency. Histopathological, pronounced diffuse cytoplasm lipid accumulation was observed, with respect to assessment of smaller and biger vacuolas in cytoplasm and dislocation and total cell damage ofhepatocite. With special histological staining method Sudan III, the accumulation of fat droplets in hepatocite was sustained. In kidney sample there were focal groups of fat droplets, which indicate on fat infiltration. In this report, two cases of fatty liver degeneration are presented and macroscopic and histopathological features are discussed.
    No preview · Article · Aug 2008
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    ABSTRACT: Concentrations of heavy metals (Pb, Cr, Mn, Fe, Cu, and Zn) were measured in water, mud and different organs (liver, kidney, intestine, spleen, skin and muscle) of bighead carp (Hypophthalmichthys molitrix), grass carp (Ctenepharyngodon idella), weis (Siluris glanis) and pike (Esox lucius) from three Croatian fish farms. All measure-merits were carried out by the source excited Energy Dispersive X-Ray Fluorescence method (EDXRF). Significant difference was found in the mud and water composition among the three fish farms. Compared to the fish farm 1, concentrations of heavy metals in mud and water from the fish farm 2 were between 2 and 6 times higher and from fish farm 3 between 2 and 5 times higher. The concentrations of heavy metals (expressed as wet weight) were measured in different organs of herbivore and predatory fish species and significant difference was found in selected heavy metals concentration in organs of two group fish species with varied modality of alimentation.
    No preview · Article · May 2008
  • Z Kozarić · S Kuzir · Z Petrinec · E Gjurcević · M Bozić
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    ABSTRACT: The European catfish, Silurus glanis L., has become an important aqua cultural fish in Croatia, and it is cultivated extensively in ponds in polyculture with carps. The development of the digestive tract in S. glanis was studied with the aim of improving intensive fish production. Research was carried out on S. glanis larval stadium from 1- to 19-day post-hatching (DPH). The main histological methods used were: haematoxylin and eosin staining, periodic acid Schiff staining (PAS), Alcian blue (AB) and toluidin blue staining (TB). A yolk sac was present during the first 5 days (1-5-DPH). During the initial 3-DPH period, there was no trace PAS and AB activity in the digestive tract. Differentiation of the digestive tract began at 3- to 5-DPH. The oesophagus was positive for AB at 5-DPH, PAS and TB after 7-DPH. Differentiation of enterocytes began at 5-DPG and the intestines were complete at 11-DPH. Development of liver and pancreas was also studied. The analysis of data obtained in this study suggests that after 5-DPH catfish larvae have morphologically completed digestive tracts.
    No preview · Article · May 2008 · Anantomia Histologia Embryologia
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    ABSTRACT: The presence of an Eimeria sp. was found in adult large-scaled gurnards Lepidotrigla cavillone (Lacepède, 1801) from the Novigrad Sea, in Croatia. The detection of Eimeria sp. in the pyloric caeca and intestines of the large-scaled gurnards is also the first report of the parasite in this fish species. The analysis of histopathological changes in the digestive tract led to the conclusion that the parasite could be a serious pathogen in heavily infected large-scaled gurnards.
    No preview · Article · Mar 2008 · Acta Parasitologica
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    ABSTRACT: Variations in the elemental composition of different carp (Cyprinus carpio) organs (liver, kidney, eggs, intestine, spleen and skin + muscle) from three Croatian fish farms were investigated. Sediments and water samples were also taken from each pond. All measurements were carried out by the source excited Energy Dispersive X-Ray Fluorescence method (EDXRF). Significant difference was found in the sediment and water composition among the three fish farms (p < 0.01). Compared to fish farm 1, concentrations of heavy metals in sediments and water from fish farm 2 were between 2 and 6 times higher and from fish farm 3 between 2 and 5 times higher. The following concentrations ranges (expressed as wet weight) were measured in different carp organs: Pb (0.11 1.45 mg/kg); Cr (0.10-3.72 mg/kg); Mn (1.70-28.47 mg/kg); Fe (8.09-117.21 mg/kg); Cu (0.41-4.09 mg/kg) and Zn (4.61-171.53 mg/kg). Significant difference was found in selected heavy metal concentrations of selected organs at each location as well as among the three locations (p < 0.05). Generally, carp from fish farm 2 had the highest concentration of the majority of elements (Pb, Cr, Mn, Fe Cu, Zn), while carp from fish farm 1 had the lowest concentration, demonstrating an impact of the geochemical environment on heavy metal concentrations in carps.
    Full-text · Article · Feb 2008 · DTW. Deutsche tierärztliche Wochenschrift
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    ABSTRACT: Nitric oxide (NO) has been implicated in the pathogenesis of migraine and treatment with its exogenous donor glyceryl trinitrate (GTN) represents widely accepted experimental »migraine model«. In this study, glyceryl trinitrate was administered intraperitoneally to carps, serum nitrite and nitrate levels were determined, permeability of blood-brain barrier was investigated, and histological changes of brain tissue were analyzed. Serum nitrite and nitrate levels displayed characteristic biphasic pattern with moderate initial increase and maximal terminal increase, suggesting the GTN-induced endogenous NO synthesis. Increased permeability of the blood-brain barrier in GTN-treated animals was determined based on Evans blue capillary leakage into the brain tissue. Histological analysis revealed changes consistent with vasodilatation and oedema. Our study strongly supports the importance of the NO role in the pathogenesis of migraine attacks and increase in blood-brain barrier permeability during the attack. The study has also provided evidence that this mechanism of action is conserved to the lower vertebrate.
    Full-text · Article · Feb 2008 · Collegium antropologicum
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    ABSTRACT: The presence of metacercariae of Centrocestus formosanus was detected on the gills of goldfish (Carassius auratus L.) imported from Singapore and obtained from two pet shops. This is the first report of the parasite in Croatia. The histopathological changes observed lead to the conclusion that the infection could be one of the reasons for the death of goldfish.
    No preview · Article · Nov 2007 · Helminthologia
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    ABSTRACT: The morphohistochemical profile of red and pink muscles from the caudal portion of the trunk at the height of the horizontal septum in the Carassius auratus gibelio and Perca fluviatilis 20-25 cm long from the Garešnica (Croatia) fish farm have been studied. Muscle samples, taken on arrival from live fish, were frozen in liquid nitrogen, cut on the cryo cut into 10 μm thick slices stained with hemalum and eosin and with the procedure for the detection of succinate dehydrogenase (SDH). The diameter of muscle fibers from the obtained preparations was measured using a micrometer scale with 10 μm divisions on a Nikon microscope. The red muscle of the Carassius auratus gibelio and Perca fluviatilis is known to have a triangular cut. It is positioned laterally beneath the skin and runs craniocaudally along the horizontal septum. Underneath the surface red muscle there is a pink muscle, shorter than the red one. In the Carassius auratus gibelio it is wide and divided into the surface (pink I) and deep (pink II) layer, while in Perca fluviatilis it is narrow, short and unchanged, but comprises a narrow transitional zone towards the red muscle. The red muscle is composed of red muscle fibers of small diameter with potent SDH activity. The pink muscle is composed of two types of fibers - intermediary small-diameter muscles of pronounced SDH activity and large-diameter muscles of less pronounced SDH activity as well as white muscles of large diameter with poor SDH activity. Intermediary fibers in the Carassius auratus gibelio accounted for 77.80% of the fibers in the pink I layer and 44.90% of the fibers in the pink II layer. White fibers in the same fish accounted for 22.20% of the fibers in the pink I layer and 55.10% of the fibers in the pink II layer. Intermediary fibers in the pink muscle of the Perca fluviatilis accounted for 58.80% fibers, whereas white fibers accounted for 41.20% of all fibers.
    No preview · Article · Nov 2007 · Medycyna weterynaryjna
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    ABSTRACT: The concentrations of cadmium, lead and zinc were determined in the soft tissue of oysters ( Ostrea edulis L.) from three locations in the Mali Ston Bay on the south-eastern coast of the Adriatic Sea. The sampling was performed during two periods of breeding cycle, in summer (age ∼1 year) and winter (age ∼20 months, con - sumption size). The concentrations of cadmium, lead and zinc measured in the oyster soft tissue showed consid - erable spatiotemporal variations that could be attributed to seasonal differences in the freshwater inflow which varied between the study locations. Median concentrations measured at different locations and in different time periods ranged from 0.303 to 0.969, 0.13 to 0.32 and 208.9 to 650.0 µg/g wet weight for cadmium, lead and zinc, respectively. Although metal concentrations do not exceed the maximal legally approved limits according to the legislation of the Republic of Croatia and EU, more knowledge of their spatiotemporal distribution - of cadmium in particular - would contribute to the sustainable future development of oyster farming in Croatian waters.
    No preview · Article · Oct 2007 · Veterinární medicína
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    Z. Kozarić · S. Kužir · Zdravko Petrinec · E. Gjurčević · N. Baturina
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    ABSTRACT: The histological structure, as well as content and distribution of glycoproteins (GPs) in the digestive tract mucosa, of the Atlantic bluefin tuna were studied. The digestive tract is short in accordance with their predatory nature and consists of the oesophagus, stomach and intestine. The short oesophagus has deep longitudinal folds and contains an abundance of secretory cells which synthesize a large quantity of neutral as well as sulphated and silylated GPs. The stomach is an elongated sac-like structure with simple tubular glands surrounded by connective tissue. The neck cells of gastric glands synthesize a mixture of neutral and acidic GPs. The intestine is short with many intestinal villi. Many goblet cells are situated between superficial epithelial cells. The intestinal goblet cells were shown to secrete both neutral and acidic GPs.
    Preview · Article · Jan 2007 · Veterinarski Arhiv