D.M. Tang

Shanghai Jiao Tong University, Shanghai, Shanghai Shi, China

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Publications (5)2.39 Total impact

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    ABSTRACT: A full-length cDNA clone encoding cytosolic glutamine synthetase (GS1; EC was isolated from melon (Cucumis melo L.) for the first time by RT-PCR and RACE approach. The clone, designated as M-GS1 (accession No. DQ851867), contains 1494 nucleotides with an open reading frame (ORF) of 1068 nucleotides. The deduced 356 amino acid sequence showed high similarity with previously reported GS1s from various plant species. Sequence analysis revealed that the predicted protein contains a GS β-Grasp domain, a GS catalytic domain, and the main conserved motifs characteristic of a plant GS1. The phylogenetic analysis displayed that M-GS1 is related most closely to the GS1 from Datisca glomerata. Southern blot analysis indicated that M-GS1 belongs to a small gene family of 2 or 3 members. M-GS1 was expressed in all plant tissues without evident tissue specificity, but with different patterns when the melon plants were fed in hydroponic culture with different forms and concentration of nitrogen. Ammonium dramatically enhanced the contents of M-GS1 transcripts in all tested tissues, while nitrate stimulated M-GS1 transcription only in the roots and leaves, but not in the stems; glutamate, however, depressed M-GS1 transcripts in the roots, but resulted in no significant change to the levels of M-GS1 transcripts in the stems and leaves. Moreover, the same effects were observed at the GS enzyme activity level. These results indicated that melons respond to changes of N nutrition by regulating M-GS1 expression. Additional key words Cucumis melo –cytosol–glutamine synthetase–nitrogen forms–relative mRNA level–total GS enzyme activity
    No preview · Article · Mar 2011 · Biologia Plantarum
  • TD Ge · DM Tang · SW Song · DF Huang

    No preview · Article · Jan 2009 · Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban
  • HM Du · DM Tang · DF Huang
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    ABSTRACT: Fragrant taro [Colocasia esculenta (L.) Schott var. antiquorum] is an important regional plant, with a good taste, from Chongming Island in the southeast of China. Its natural vegetative rate of propagation is relatively low and no research on in vitro propagation of 'fragrant taro' has been reported. Thus, an in vitro propagation method for 'fragrant taro' was developed, and the effects of thidiazuron (TDZ) and benzylaminopurine (BAP) on shoot-tip differentiation and multiplication were analysed. Shoot-tips were first cultured in half-strength Murashige and Skoog (MS) semi-solid medium containing different concentrations of TDZ, BAP and naphthalene acetic acid (NAA) for 30 d. The best result was found with 1.0 mg l-1 TDZ treatment. Plantlets were then sub-cultured in MS liquid medium for another 90 d. After 30 d, the highest multiplication rate (2.5) was observed in medium with 1.0 mg l -1 BAP and 0.5 mg l-1 NAA. The multiplication rate in medium supplemented with 3.0 mg l-1 BAP and 0.1 mg l-1 TDZ was the highest (4.7) after 90 d of culture. Well-developed shoots were rooted in MS medium solidified with 0.6% (w/v) agar, supplemented with 1.0 mg l -1 BAP, 0.5 mg l-1 NAA and 500 mg l-1 active carbon before transplanting. A protocol for propagating 'fragrant taro' in vitro was established in which shoots maintained rapid multiplication for a long time. This protocol can also be used for germplasm conservation and exchange of 'fragrant taro'.
    No preview · Article · May 2006 · Journal of Horticultural Science and Biotechnology
  • H.M. Du · D.M. Tang · D.F. Huang

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  • H.M. Du · M. Ding · D.M. Tang · D.F. Huang

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