T V Sirota

Institute of Theoretical and Experimental Biophysics, Pushchino-na-Oke, Moskovskaya, Russia

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Publications (31)22.95 Total impact

  • T V Sirota
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    ABSTRACT: An important role of carbonate/bicarbonate ions has been recognized in the superoxide generating reaction of adrenaline autooxidation in an alkaline buffer (a model of quinoid adrenaline oxidation in the body). It is suggested that these ions are directly involved not only in formation of superoxide anion radical (O 2 −· ) but also other radicals derived from the carbonate/bicarbonate buffer. Using various buffers it was shown that the rate of accumulation of adrenochrome, the end product of adrenaline oxidation, and the rate of O 2 −· formation depend on concentration of carbonate/bicarbonate ions in the buffer and that these ions significantly accelerate adrenaline autooxidation thus demonstrating prooxidant properties. The detectable amount of diformazan, the product of nitro blue tetrazolium (NBT) reduction, was significantly higher than the amount of adrenochrome formed; taking into consideration the literature data on O 2 −· detection by NBT it is suggested that adrenaline autooxidation is accompanied by one-electron reduction not only of oxygen dissolved in the buffer and responsible for superoxide formation but possible carbon dioxide also dissolved in the buffer as well as carbonate/bicarbonate buffer components leading to formation of corresponding radicals. The plots of the dependence of the inhibition of adrenochrome and diformazan formation on the superoxide dismutase concentration have shown that not only superoxide radicals are formed during adrenaline autooxidation. Since carbonate/bicarbonate ions are known to be universally present in the living nature, their involvement in free radical processes proceeding in the organism is discussed.
    No preview · Article · Feb 2015
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    ABSTRACT: In this paper the oil from seeds of Amaranthus cruentus L. (AmO) was shown to be an efficient modulator of the physical chemical properties of artificial lipid and rat hepatocyte plasma membranes. AmO improved the membrane stability, their stress resistance and the adsorption of neurotensin to plasma membranes with the distinct biphasic interactions being observed even after adrenalin stress exposure. The analysis of pro-/antioxidant balance in rat blood revealed a mild prooxidant activity after AmO intake, which was accompanied by accumulation of oxidative destruction products in plasma membranes. This prooxidant action of AmO was corroborated in vitro in an adrenalin autooxidation model. On the other hand, the observed improved resistance to adrenalin stress in AmO supplemented rats was associated with an antioxidant response in blood and plasma membrane studies. The AmO effects can be attributed to the modulation of the metabolic pathways involved into oxygen and free radical homeostasis.
    Full-text · Article · Mar 2014 · Food Chemistry
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    ABSTRACT: Amaranth oil has several health benefits. It has lipid lowering, anti-diabetic, immune modulatory and cytoprotective properties, activates the function of mitochondria and improves heart rate variability. It has been suggested that the effect of amaranth oil on redox status is involved in this multitude of cellular and clinical influences of the oil. We examined whether amaranth oil can modify free radical production. EPR experiments with amaranth oil dissolved in DMSO showed scavenging of carbon centered radicals but not of oxygen centered radicals. Moreover, a concentration dependent scavenging effect of amaranth oil on ultrasound-induced radicals was observed. However, in adrenaline autoxidation experiments amaranth oil showed a strong prooxidant action through activation of superoxide anion formation. This two-sided effect of amaranth oil, i.e. both anti- and pro-oxidant action, was corroborated in human lung fibroblasts that were exposed to amaranth oil. At low concentrations of amaranth oil, fibroblasts were protected against oxidative stress, whereas in incubations with high amaranth oil concentrations more H2O2-induced intracellular radical damage was found. We suggest that mild pro-oxidant activity could be the underlying mechanism in the health beneficial effect of amaranth oil.
    Full-text · Article · Dec 2013
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    ABSTRACT: Several parameters of the cytoplasmic enzymatic antioxidant system of the liver and brain of the rat have been investigated under conditions of immobilization stress and of an antioxidant preparation in the diet of animals. These included superoxide dismutase (SOD) and glutathione reductase (GR) activities and nonspecific NADPH oxidation. Only changes in the activity of SOD both in the liver and brain were revealed. In the liver of animals that receive no preparation, a decrease in the activity of SOD after 30-min immobilization and its restoration after a 360-min immobilization were observed. In the brain, the activity of SOD decreased only in preconditioned animals after 30 and 360 min of exposure to stress. In addition, the activity of SOD in the brain of preconditioned animals, both stressed and unstressed, was lower than in the corresponding groups of control animals. It is probable that, under the conditions of immobilization stress, the level of reactive oxygen species (ROS) and as a consequence the activity of SOD decrease. The intake of an antioxidant preparation under these conditions seems to be not correct.
    No preview · Article · Mar 2013 · Biochemistry (Moscow) Supplement Series B Biomedical Chemistry
  • T. V. Sirota
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    ABSTRACT: The addition of nitro blue tetrazolium (NBT) into the reaction of adrenaline autooxidation allows direct identification of superoxide anion formation (O 2−⊙) as well as demonstration of kinetics of their accumulation in this superoxide-generating system. The kinetics of adrenochrome and O 2−⊙ formation has been compared under the same conditions. Three possible approaches to the use of the adrenaline autooxidation reaction for the determination of superoxide dismutase activity (SOD) and revealing antioxidant properties of various compounds are discussed. Two of these approaches have been described previously: the spectro-photometric method of registration of adrenochrome, an end product of adrenaline autooxidation, at 347 nm (Sirota, 1999) and the polarographic method, which measures oxygen consumption used for O 2−⊙ formation (Sirota, 2011). Here, a novel approach to this problem is presented; it is based on spectrophotometric determination of O 2−⊙ using NBT. The employment of this approach results in a significant decrease of the pH value of carbonate buffer from 10.5 to 9.7 and a 4-fold decrease in the amounts of added adrenaline, thus creating milder conditions for the revealing and investigation of antioxidant properties of materials being examined.
    No preview · Article · Jul 2012 · Biochemistry (Moscow) Supplement Series B Biomedical Chemistry
  • T V Sirota
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    ABSTRACT: The reaction of adrenaline autoxidation in an alkaline buffer with the formation of superoxide radicals and the product of its oxidation, adrenochrome, which models the quinoid pathway of adrenaline conversion in the body, is accompanied by oxygen consumption. This reaction is applicable for polarographic determination of the activity of superoxide dismutase and the antioxidant properties of biological and chemical compounds, it is based on evaluation of the latent period and the rate of oxygen consumption, which are measured in the presence of the compounds examined. It was assumed that the neuro- and cardiotoxicity of quinone products of adrenaline oxidation is related not only to their "own" properties and reactive oxygen species formed but also the hypoxia of those regions of the cell and tissue where the quinoid oxidation of adrenaline occurs.
    No preview · Article · Jan 2012
  • T. V. Sirota
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    ABSTRACT: The reaction of adrenaline autooxidation in an alkaline carbonate buffer followed by formation of superoxide radicals and the product of its oxidation, adrenochrome, which models the quinone pathway of adrenaline metabolism in the body, is accompanied by oxygen consumption. A study of this process by the polarographic method enabled one to apply this reaction to determine the activity of superoxide dismutase and antioxidant properties of biological and chemical compounds; it is based on evaluation of a latent period and the rate of oxygen consumption, which are measured in the presence of examined compounds. It was suggested that known neuroand cardiotoxicity of the quinone products of adrenaline oxidation may be associated not only to their intrinsic properties and reactive oxygen species formed but also local hypoxia of those regions of the cell and tissue where the quinone oxidation of adrenaline occurs. Keywordsadrenaline–adrenochrome–superoxide–oxygen–polarography–superoxide dismutase
    No preview · Article · Sep 2011 · Biochemistry (Moscow) Supplement Series B Biomedical Chemistry

  • No preview · Article · Mar 2011 · Doklady Chemistry
  • T V Sirota · A I Miroshnikov · K N Novikov
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    ABSTRACT: The ability of water preparations of different composition to affect the generation of superoxide radicals has been compared. The superoxygenating reaction of adrenaline autoxidation with some modifications was used as a model system, which makes it possible to reveal the pro/antioxidant properties of materials being tested. It was shown that samples of water from sources having different specific electroconductivity and, accordingly, ionic composition differ in the ability to affect reactions proceeding with the participation of ROS. The parameter measured, the pro/antioxidant activity of water, is a new informative indicator, and the approach proposed enables one to perform a comparative estimation of the quality of water and aqueous solutions.
    No preview · Article · Dec 2010 · Biofizika
  • T V Sirota
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    ABSTRACT: The antioxidant system of some tissues in guinea pigs with no selenium deficiency was studied after treatment with selenium-containing products. Activity of a selenium-containing enzyme glutathione peroxidase was significantly reduced in the kidneys and blood. The kidneys were characterized by low activity of glutathione reductase and high activity of SOD. These features illustrate the development of oxidative stress in the kidneys.
    No preview · Article · Oct 2010 · Bulletin of Experimental Biology and Medicine
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    ABSTRACT: The effect of ionized air containing negatively charged ions at a concentration of 320000–350000 ions/cm3 inhaled by rats was studied. It was demonstrated that the inhalation of negative air ions for 60 min activated the secretion of goblet cells without impairing the tracheal mucosa and changing the protein profile of bronchoalveolar lavage. It was also found that the level of spontaneous production of reactive oxygen species by unfractionated blood cells increased after the action of negative air ions in both males and females. However, the intensity of their generation induced by opsonized zymosan increased only in females. Different sensitivities of the female and male blood antioxidant enzymes—superoxide dismutase and glutathione reductase—to negative air ions were observed. These results allow the effect of negative air ions on the respiratory organs and blood to be interpreted as priming and weak activation via a direct action on the mucosa of primary target respiratory organs and then on the blood.
    Full-text · Article · Sep 2008 · Biophysics
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    ABSTRACT: The effect of negatively charged ions on respiratory organs and blood of rats has been studied. It was shown that the inhaling of negative air ions (NAI) for 60 min with a concentration of NAI at the place of location of animals 320-350 000 ions/cm2 activated the secretion of goblet cells without damaging the mucosa of the trachea and changed the spectrum of proteins of bronchopulmonary lavage. It was also found that the spontaneous production of reactive oxygen species (ROS) by cells of nonfractionated blood after the exposure to NAI increased in both males and females; the intensity of ROS generation induced by opsonized zymosan increased only in females. Different sensitivity of the antioxidant enzymes superoxide dismutase and glutathione reductase of blood to NAI in females and males was revealed. These results enable one to consider the effect of NAI as priming and a weak activation of the respiratory organs through the direct action on the mucosa of the primary target organs of the respiratory tract and then on the blood.
    No preview · Article · Sep 2008 · Biofizika
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    ABSTRACT: Respiration parameters of liver mitochondria (MCh) in rats fed with amaranth seed oil for 3 weeks have been evaluated. Thirty minutes before decapitation, adrenaline was injected intraperitoneally at a low dose (350 μg/kg body weight) to both control and experimental animals. It was shown that in animals that were injected with adrenaline and did not receive oil, the rate of phosphorylating respiration increased by 32% and phosphorylation time decreased by 22% upon oxidation of succinate; upon oxidation of α-ketoglutarate in the presence of the succinate dehydrogenase inhibitor malonate, phosphorylating respiration was activated by 23%. The respiration of MCh upon oxidation of succinate + glutamate and α-ketoglutarate in the absence of malonate was not affected by adrenaline. The intake of oil markedly activated almost all parameters of mitochondrial respiration in experimental rats upon oxidation of all above-listed substrates in both coupled and uncoupled MCh. However, phosphorylation time was close to the control value (upon oxidation of succinate) or increased (upon oxidation of α-ketoglutarate in the presence and absence of malonate). The injection of adrenaline to animals receiving oil did not affect the oil-activated respiration of MCh oxidizing the substrates used; however, phosphorylation time in all groups of animals decreased. Ca2+ capacity of MCh in rats receiving amaranth oil did not change. Thus, our data show that feeding of rats with amaranth oil activates mitochondrial respiration and prevents MCh hyperactivation induced by adrenaline.
    Full-text · Article · Feb 2008 · Biochemistry (Moscow) Supplement Series A Membrane and Cell Biology
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    ABSTRACT: We studied behavioral reactions of rats after injection of subconvulsive dose of kainic acid into the frontal cortex and mitochondrial respiration in the hippocampus and frontal and temporal cortex 17-20 days after administration of kainic acid. Retention of acquired habit and the dynamics of its extinction in experimental rats were close to those in the control group. Changes in mitochondrial function were observed only in the region of kainic acid injection: activation of phosphorylating respiration during oxidation of succinate. Presumably, the detected activation of energy metabolism in the frontal cortex indicates functional restructuring in mitochondria, aimed at compensation of disorders caused by the neurotoxin.
    No preview · Article · Dec 2007 · Bulletin of Experimental Biology and Medicine
  • V I Arkhipov · T V Sirota · D S Lebedev
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    ABSTRACT: Cognitive processes and functional state of mitochondria in brain structures of Wistar rats were studied after intrahippocampal injection of kainic acid, an agonist of glutamate receptors. A single administration of 0.25 microg kainic acid into the dorsal part of the left and right hippocampi affected task retrieval and decreased inhibition of unrewarded responses. The injection of 0.75 microg kainic acid induced recurrent seizures and completely disorganized animal behavior. The functional state of mitochondria, as an important marker of excitotoxicity, was studied after intrahippocampal injections of kainic acid in the same doses. Kainic acid at 0.25 microg proved to activate the oxidative phosphorylation in hippocampal mitochondria. A higher (epileptogenic) dose of kainic acid inhibited mitochondrial respiration in the frontal cortex, but had an insignificant effect on mitochondrial respiration in the hippocampus. The disturbed interaction between the hippocampal system and frontal cortex after kainic acid administration can be the main factor of the revealed cognitive dysfunctions.
    No preview · Article · Oct 2007 · Izvestiia Akademii nauk. Seriia biologicheskaia / Rossiiskaia akademiia nauk
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    ABSTRACT: It has been shown that a three-week feeding of rats with oil derived from seeds of amaranth (Amaranthus cruentus L.) leads to a moderate activation of respiration of coupled and uncoupled rat liver mitochondria (MCh) that oxidize succinate and succinate + glutamate, as well as alpha-ketoglutarate and alpha-ketoglutarate + malonate. In animals receiving the amaranth oil, the injection of adrenaline did not affect the oil-activated respiration of MCh during succinate oxidation; i. e., animals prepared by an oil-enriched diet were resistant to the action of adrenaline, which prevented from possible hyperactivation of mitochondrial functions. In the group of control animals, which received no oil, the injection of adrenaline activated the rate of phosphorylating respiration of MCh during oxidation of succinate or succinate + glutamate: the rate of oxygen uptake in state 3 respiration (by Chance) increased, and the phosphorylation time decreased. The injection of adrenaline did not affect the parameters of respiration of MCh that oxidize a-ketoglutarate; however, in the presence of malonate, the oxidation of alpha-ketoglutarate in state 3 and uncoupled respiration have shown mild but significant increase in response to adrenaline. In animals receiving the amaranth oil, the oil-induced activation of respiration of MCh in response to adrenaline retained but did not increase; however, the phosphorylation time significantly decreased. Thus, concentrated oil of seeds activates the respiration of MCh. In addition, it enhances an energetic function of MCh, which prevents from the hyper-activation of mitochondrial respiration by adrenaline. Therefore an activation of energetic function of MCh by amaranth oil could explain its adaptogenic effect on rats.
    No preview · Article · Jan 2007 · Ukrainskii biokhimicheskii zhurnal
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    ABSTRACT: It has been shown that a three-week feeding of rats with oil derived from seeds of amaranth (Amaranthus cruentus L.) leads to a moderate activation of respiration of coupled and uncoupled rat liver mitochondria (MCh) that oxidize succinate and succinate + glutamate, as well as α-ketoglutarate and α-ketoglutarate + malonate. In animals receiving the amaranth oil, the injection of adrenaline did not affect the oil-activated respiration of MCh during succinate oxidation; i. e., animals prepared by an oil-enriched diet were resistant to the action of adrenaline, which prevented from possible hyperactivation of mitochondrial functions. In the group of control animals, which received no oil, the injection of adrenaline activated the rate of phosphorylating respiration of MCh during oxidation of succinate or succinate + glutamate: the rate of oxygen uptake in state 3 respiration (by Chance) increased, and the phosphorylation time decreased. The injection of adrenaline did not affect the parameters of respiration of MCh that oxidize α-ketoglutarate; however, in the presence of malonate, the oxidation of α-ketoglutarate in state 3 and uncoupled respiration have shown mild but significant increase in response to adrenaline. In animals receiving the amaranth oil, the oil-induced activation of respiration of MCh in response to adrenaline retained but did not increase; however, the phosphorylation time significantly decreased. Thus, concentrated oil of seeds activates the respiration of MCh. In addition, it enhances an energetic function of MCh, which prevents from the hyper-activation of mitochondrial respiration by adrenaline. Therefore an activation of energetic function of MCh by amaranth oil could explain its adaptogenic effect on rats.
    Full-text · Article · Jan 2007
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    ABSTRACT: It was shown for the first time that inhaling negative air ions (NAI) leads to changes in the functional state of the rat trachea mucosa, which in turn affects the phagocytic activity of blood cells. The effect depends on the concentration of the NAI, the duration of their action, and the sources of the NAI generation. For air ionization, technical ionizers and a bioionizer (a plant electrified through soil) developed by the Diod Company, Moscow, Russia were used. It was shown that the NAI at high doses (600 000 ion/cm<sup>3</sup>), generated by an Elion 131M device, induce a damage to the rat trachea mucosa, an inhibition of the activity of its antioxidant enzymes, and a decrease in the phagocytic activity of whole blood cells. Low doses of the NAI from the same source (25 000-50 000 ion/cm<sup>3</sup>) and high doses of the NAI from devices Elion 132S and 132R (320 000-500 000 ion/cm<sup>3</sup>), and a bioionizer (500 000 ion/cm<sup>3</sup>) produced a milder effect, activating the secretion of goblet cells of the trachea mucosa without its damage. A possible mechanism of the effect of inhaled NAI as exogenous reactive oxygen species (ROS) on internal processes in the organism is discussed. The involvement of the ROS in the action of ionized air found in the study is probably the general property of different electrical influences on biological objects. Therefore, the result of the investigation may be of interest for a wide circle of specialists dealing with ElectroMed influences
    No preview · Article · Sep 2006 · IEEE Transactions on Plasma Science
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    ABSTRACT: Generation of negative air ions (NAI) by pot plants (aloe Aloe arborescens, haworthia Haworthia rasalata, echinopsis Echinopsis tubiflora, mammillaria Mammillaria prolifera, opuntia Opuntia brunnescens, spider plant Chlorophytum comosum, and jade plant Crassula portulacea) was studied when high-voltage pulses were applied to soil. Plants that generated low amounts of NAI (such as jade plant and echinopsis) elevated NAI level 2–3 times over the mean background level. Plants that produced moderate amounts of NAI (haworthia, opuntia, and mammillaria) increased the NAI level hundredfold, whereas highly active plants (aloe and spider plant) increased the NAI level thousand times. Aloe plants can maintain constant NAI concentration in the ambient air (125 15 103 ions/cm3) for a long time (7–8 h). Negative air ions were predominantly generated by the leaf tips. The capacity of aloe to produce NAI considerably reduced with plant age and was lost, although reversibly, at temperatures below 8.5C. The rate of NAI generation considerably depended on weather conditions. It was demonstrated that superoxide anion radical is one of NAI species generated by plants.
    No preview · Article · Apr 2004 · Russian Journal of Plant Physiology
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    ABSTRACT: The role of impairment of general oxidative and energy metabolism in pathogenesis of lower urinary tract symptoms (LUTS) in patients with benign prostatic hyperplasia (BPH) and their correction by (1-adrenoblocker alfuzosin was studied. One group of patients (N = 126) was examined by standard methods for determination of the severity of LUTS by IPSS and mean effective volume of urinary bladder (MEVUB). In the second group (N = 29) in addition to functional examinations, metabolic indicators in blood were measured: antioxidant activity (AOA) and succinate dehydrogenase activity (SDA). Severity of LUTS depends greatly on the MEVUB. It was the first to show a practically complete correlation between LUTS, AOA and SDA. Severity of LUTS exactly correlates with indicators of oxidative and energy metabolism. In patients with more heavy LUTS, lowest AOA and SDA values were found. In the course of effective treatment, both phenomena developed an improvement of clinical symptoms and a rise of biochemical parameters. Close correlation between functional and metabolic phenomena is evidence of an essential role of metabolic mechanisms in the pathogenesis of LUTS with BPH. This opens perspectives to use antioxidants and energy metabolism activators for correction of UB dysfunction in patients with BPH.
    No preview · Article · Nov 2003 · Mitochondrion

Publication Stats

176 Citations
22.95 Total Impact Points

Institutions

  • 1997-2015
    • Institute of Theoretical and Experimental Biophysics
      Pushchino-na-Oke, Moskovskaya, Russia
  • 2000-2013
    • Russian Academy of Sciences
      • Kurnakov Institute of General and Inorganic Chemistry
      Moskva, Moscow, Russia
  • 2008
    • Danylo Halytsky Lviv National Medical University
      Lviv, Lviv, Ukraine