Dan-Wei Ma

Sichuan Normal University, Hua-yang, Sichuan, China

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Publications (3)0.57 Total impact

  • Wan-Jun Hu · Dan-Wei Ma · Ya-Nan Wang · Hong Zhang
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    ABSTRACT: Chenopodium ambrosioides is an invasive species, which has strong allelopathic effect on surrounding plants. In this study, the methods of soil culture and filter paper culture were adopted to simulate the eluviation and volatilization of the volatile oil from C. ambrosioides, respectively, and to investigate the allelopathy of the volatile oil on the lipid peroxidation and antioxidant enzyme activities of Vicia faba root tip cells, with the mechanisms of the induced tip cell apoptosis analyzed. At the early stage (24 h) of soil culture and filter paper culture, the superoxide dismutase, peroxidase and catalase activities of the tip cells decreased after an initial increase with the increasing dose of the volatile oil, and the malondialdehyde content of the tip cells increased with the increasing volatile oil dose and treated time. At the midterm (48 h) and later (72 h) stages of soil culture and filter paper culture, a typical DNA ladder strip appeared, suggesting that the volatile oil from C. ambrosioides could induce the apoptosis of the tip cells, and the apoptosis was dose- and time dependent. This study showed that the volatile oil from C. ambrosioides could act on its surrounding plants via eluviation and volatilization, making the lipid peroxidation of acceptor plants aggravated and the antioxidant enzyme activities of the plants inhibited, resulting in the oxidative damage and apoptosis of the plant root tip cells, and accordingly, the inhibition of the plant growth. Under soil culture, the root tip cells of V. faba had higher antioxidant enzyme activities and lesser DNA damage, suggesting that the volatile oil from C. ambrosioides via volatilization had stronger allelopathy on the growth of surrounding plants than via eluviation.
    No preview · Article · Apr 2012 · Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban
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    ABSTRACT: species level. Observed number of alleles was 1.9093 ± 0.2875 and effective number of alleles was 1.4048 ± 0.3342. Nei's gene diversity and Shannon's Information index were 0.2494 ± 0.1657 and 0.3903 ± 0.2186, respectively. Nei's genetic distance among 22 populations ranged from 0.0996 to 0.6952. Genetic identity among populations ranged from 0.4990 to 0.9052, averaging 0.7387. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis based on Nei's genetic distance indicated that most populations were positioned into the relevant area. The above-mentioned results suggested the level of genetic variation of P. paniceum paniceum (Lam.) Hack. was low. The high score of PPB might be caused by low frequent polymorphism distributed in different populations. A significant correlation between genetic and geographic distances of populations was revealed based on Mantel test.
    Full-text · Article · Jan 2010 · AFRICAN JOURNAL OF BIOTECHNOLOGY
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    ABSTRACT: Using degenerate primers and RT-PCR, RACE techniques, a 1491 bp cDNA segment of stearoyl-acyl carrier protein desaturase (SAD) is cloned from developing seeds of Jatropha curcas L. The segment contains a 1191 bp of complete open reading frame (ORF). Analysis in the BLAST on NCBl shows that Jatropha curcas SAD (JSAD) gene encodes a protein precursor composed of a signal peptide of 33 amino acids and a mature peptide of 363 amino acids. The homological analysis shows that JSAD has high level of homology both in nucleotide sequence and in amino acid sequence to other plants SADs. The nucleotide and peptide identity of JSAD to Ricinus communis SAD (RSAD) is up to 89% and 96.2% respectively. Molecular modeling of JSAD indicates that its three-dimensional structure strongly resembled the crystal structure of RSAD.
    No preview · Article · Apr 2007 · Journal of Shanghai University (English Edition)