Yingying Huang

Zhejiang University, Hang-hsien, Zhejiang Sheng, China

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Publications (2)3.84 Total impact

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    ABSTRACT: In this paper we focus our attention on RIXI, a member of the XIP type xylanase inhibitor proteins in rice. RIXI-overexpression transgenic lines were generated by expressing the RIXI gene under the control of auliflower mosaic virus 35S promoter. In comparison with the wild-type (WT) plants, the transgenic plants had significantly increased levels of RIXI and showed resistance to Magnaporthe oryzae. Transgenic plants also contained higher levels of H2O2 and had larger changes in catalase and superoxide dismutase activities than the WT plants. The results showed that the increase in RIXI expression was accompanied by the up-regulation of pathogenesis-related genes and genes related to the jasmonate signaling pathway. To clarify the expression pattern of RIXI, a ProRIXI: GUS vector was constructed and transgenic rice lines were obtained. GUS staining results suggested that the RIXI gene possessed distinctive, tissue-specific and grow stage-specific expression patterns in rice. This is the first report on the expression patterns of rice xylanase inhibitors and our results provide direct evidence, at the plant level, that xylanase inhibitors are involved in plant defense against fungal pathogens.
    No preview · Article · Dec 2014 · Plant Cell Tissue and Organ Culture
  • Xiaoyan Weng · Yingying Huang · Chunxiao Hou · Dean Jiang
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    ABSTRACT: Background: Xylanases have attracted considerable interest in recent years owing to their various applications in industry and agriculture. The use of transgenic plants to produce xylanases is a less expensive alternative to biotechnological programmes. The aim of this study was to elucidate whether introducing a foreign xylanase gene ATX into rice had any adverse effect on plant growth and development. Results: A recombinant xylanase gene ATX was introduced into rice variety Zhonghua 11 through Agrobacterium-mediated transformation. The T₂ generation of transgenic rice was compared with the control (non-transgenic plants). Exogenous xylanase gene ATX was expressed in rice, and all examined transgenic lines exhibited xylanase activity. The transgenic lines (T₂, 'X1-3' and 'X2-5') appeared to grow and develop normally. There were no differences in net photosynthetic rate between transgenic rice lines ('X1-3' and 'X2-5') and wild type (WT) rice plants at the heading/flowering stage. Xylanases are key enzymes in the degradation of plant cell walls. Cell wall composition analysis showed that that there were no changes in cell wall polysaccharides in the root apex but some alterations in leaves in transgenic rice plants. The results also showed that the expression of exogenous xylanase gene ATX in rice would increase the expression of endogenous xylanase inhibitor gene RIXI, which could play a role in plant defence. Thus the stress resistance of transgenic rice plants might be improved. Conclusion: Exogenous xylanase gene ATX could be successfully expressed in rice, and the exogenous protein had no apparent harmful effects on growth and development in transgenic rice plants.
    No preview · Article · Jan 2013 · Journal of the Science of Food and Agriculture