[Show abstract][Hide abstract] ABSTRACT: Ultrastructure of Helicobacter pylori were studied by transmission electron microscopy in freshly isolated and ex vivo cultured biopsy specimens of gastric antral mucosa from patients with duodenal ulcer.
No preview · Article · Apr 2001 · Bulletin of Experimental Biology and Medicine
[Show abstract][Hide abstract] ABSTRACT: Transmission electron microscopy was used to study the ultrastructural organization of circulating immune complexes (CIC), isolated from patients with typhoid fever in the different periods of acute infectious process (febrile period, period of an early and late reconvalescence), relapse and from acute and chronic carriers of Salmonella typhi in the period of pathogen excreting. It has been shown that preparations of CIC from healthy donors consisted of amorphous mean electron density material, including a cell-like detritus. At acute and chronic infectious process there were bacterial cells in a structure of the CIC. Depending on the period of disease, bacteria had different ultrastructural organization in the CIC. In the febrile period, in the period of an early reconvalescence and in the period of formation of acute carriage of S. typhi, and also in relapse, bacteria had a typical structure, with reference to gram-negative microorganisms. In the period of formation of acute and chronic carriers of S. typhi, in a period of excretion of S. typhi, bacteria in the CIC had ultrastructural organization, relevant to the forms of bacteria with a defective cell wall. Immunocytochemistry research made with the purpose of visualization the O-antigen of S. typhi in bacteria has demonstrated positive immunolabeling on the O-antigen in the bacterial forms and forms with a defective cell wall, and on amorphous mean electron density material. The analysis of ultrastructural organization of the circulating immune complexes and immunolabeling on the O-antigen of S. typhi have allowed to conclude that S. typhi, both typical bacterial form, and the forms with a defective cell wall were the main structural component of circulating immune complexes in acute and chronic forms of infectious process.
No preview · Article · Aug 2000 · Russian journal of immunology: RJI: official journal of Russian Society of Immunology
[Show abstract][Hide abstract] ABSTRACT: An original procedure which permits to isolate circulating immune complexes (CIC) from the blood plasma in a form of a dense pellet was developed. This procedure was applied for the ultrastructural analysis of CIC isolated from blood of healthy blood donors and patients suffering from Yersinia enterocolitica (YE) and Yersinia pseudotuberculosis (YP) infections. The here reported method of CIC isolation from blood plasma permitted to visualize CIC electronmicroscopically as amorphous masses of low, middle, and high electron density with inclusions of cell debris. In contrast to CIC of healthy blood donors, CIC of infected patients contained various bacteria and fungiformic structures. For the first time, this method made possible an ultrastructural demonstration of bacterial destruction outside of phagocytes in vivo. This method also permits to visualize and identify bacteria in cases of lingering forms of infection when hemoculture tests fail. Therefore, electronmicroscopic examination of CIC preparations from the blood plasma might be a very informative indicator of bacteriemia in the course of an infection process and serve as an indicator of therapeutic effects. In lingering forms of an infection process, ultrastructural analysis of CIC preparations can be of prognostic value and serve as an indicator of therapeutic effects. This method might be also advantageous as an additional test for the exposure of latent bacterial persistence in diagnostically complicated cases.
No preview · Article · Jan 1995 · Journal of Basic Microbiology