Mi Ran Roh

Korea University, Sŏul, Seoul, South Korea

Are you Mi Ran Roh?

Claim your profile

Publications (1)1.56 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: We investigated the effects of puerarin (8-C-glucosyl-7,4′-dihydroxy isoflavone), an isoflavone found in Kudzu roots (Pueraria lobata), and its glycosides (enzymatically synthesised, water-soluble derivatives of puerarin) on melanogenesis in vitro. Puerarin and its glycosides reduced mushroom tyrosinase activity by 88 and 67% at 4.8mM, respectively, in a concentration-dependent manner. The puerarin glycosides were less effective than puerarin at the same concentration but showed a comparable inhibitory effect at a concentration at which puerarin is insoluble in water. In cultured B16 melanocytes, the melanin content was reduced significantly; moreover, tyrosinase activity was inhibited significantly by both puerarin and its glycosides when added at a concentration of 480μM. DNA microarray and RT-PCR analyses showed significant downregulation of the expression of microphthalmia-associated transcription factor (MITF) and its target genes. The protein expression of MITF and tyrosinase was also downregulated significantly by 40 and 50%, respectively. Our findings suggest that puerarin and its glycosides cause hypopigmentation via dual mechanisms: by inhibiting tyrosinase activity directly and by altering the expression of melanogenesis-related genes, such as MITF and tyrosinase. Therefore, puerarin and its glycosides may have potential for the development of functional cosmetics causing hypopigmentation. KeywordsPuerarin-Melanogenesis-Hypopigmentation-Tyrosinase-Melanin-B16 murine melanocytes-Microarray analysis
    No preview · Article · May 2010 · European Food Research and Technology