Marija Božić

Klinički centar Srbije, Beograd, Central Serbia, Serbia

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Publications (7)10.9 Total impact

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    ABSTRACT: In the current study, a rapid and sensitive LC-QTOF-MS/MS method for the determination of brinzolamide in dried blood spots (DBS) was developed and validated. This novel sample collection, storage and transfer technique was suitable for analyzing a drug with high distribution into red blood cells and negligible plasma levels. The method included an isocratic mobile phase consisting of methanol and 10. mM ammonium formate (90:10, v/v) and detection in positive electrospray mode (ESI+). The flow rate was adjusted to 0.350. mL/min yielding retention times of 1.7. min for both brinzolamide and internal standard (IS) rabeprazole on a Cyano analytical column, respectively. The validation of the proposed method over the concentration range 0.500-20.0. μg/mL was performed in compliance with EMEA and FDA guidelines, assessing all major performance characteristics. Inter- and intra- assay precisions were less than 14%, while inter- and intra- assay accuracies varied from 92.2 to 111%. No matrix effect was observed and the mean brinzolamide extraction recovery was 93.5%. The method was successfully applied to real DBS samples from patients in steady state condition, receiving brinzolamide ophthalmic suspension 1% (w/v) for several months. Initial concentrations were corrected due to hematocrit effect, using image processing algorithm written in Matlab.
    No preview · Article · Nov 2015 · Journal of pharmaceutical and biomedical analysis
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    ABSTRACT: Purpose: To determine ocular pulse volume values in patients with thyroid-associated ophthalmopathy with normal intraocular pressure, and to test the hypothesis that changes in orbital tissue that accompany thyroid-associated ophthalmopathy can in turn give changes in choroidal perfusion. Methods: In a prospective study, we evaluated 30 eyes of 30 consecutive patients with TAO, and 30 eyes of 30 healthy subjects. Complete ophthalmologic examination including dynamic contour tonometry was done. Possible differences in ocular parameters between the tested groups were assessed. Results: . No significant difference was found in ocular pulse volume values between the tested subjects (paired test p=0.23). Conclusions: . The orbit tissue changes that are involved in thyroid-associated ophthalmopathy do not have much implication on choroidal perfusion, at least when intraocular pressure values remain within the normal range.
    Full-text · Article · Nov 2012 · European journal of ophthalmology
  • Marija Božić · Miroslav L Dukić · Milenko Stojković
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    ABSTRACT: Purpose: To investigate the spectral content of intraocular pressure (IOP) pulse wave by advanced spectral signal processing of continuous IOP readings obtained by dynamic contour tonometry. Patients and methods: A non-interventional case-control study included 20 healthy subjects, 20 previously untreated primary open angle glaucoma patients, and 20 previously untreated normal tension glaucoma patients. The continuous IOP reading obtained by dynamic contour tonometry was submitted to Fast Fourier Transform signal analysis and further statistical data processing. Results: The spectral components of the IOP pulse wave were discerned up to the fifth harmonic. Highly statistically significant difference was found between the control group and the primary open angle group, and between the primary open angle glaucoma group and the normal tension glaucoma group in the first, second and the third harmonic amplitude (p < 0.01). Glaucoma patients had significantly higher ocular pulse volume values. Conclusions: It is possible to determine spectral components of the IOP pulse wave up to the fifth harmonic by a spectral analysis of dynamic contour tonometry continuous readings. We found that high Ocular Pulse Amplitude values in primary open angle glaucoma group was associated with high harmonics amplitude, which indicates low rigidity of blood vessels.
    No preview · Article · Jun 2012 · Current eye research
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    Full-text · Article · May 2012 · Albrecht von Graæes Archiv für Ophthalmologie
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    ABSTRACT: There has been only 1 study on postoperative pain after external dacryocystorhinostomy (DCR) that compared pain between 2 groups of patients; 1 group received local anesthesia and the other received general anesthesia. To further characterize the relationship between these 2 types of anesthesia and postoperative pain, we designed a study in which a single patient received these 2 different anesthesia modalities for a short interval on 2 different sides. There were 50 participants in this study. External DCR was performed on the same participant on both sides using local anesthesia on 1 side and general anesthesia on the other. Postoperative pain was measured using the visual analogue scale (VAS), and localization and timing of pain were reported by the participants. Postoperative nausea and vomiting (PONV) were documented if present. Pain levels were significantly higher with general anesthesia 3 hours post-surgery, and 6 hours post-surgery the pain remains higher following general anesthesia but is borderline insignificant (p=0.051). However, 12 hours post-surgery, there is no significant difference in the pain level (p=0.240). There was no significant difference in the localization of pain with local and general anesthesia. Postoperative nausea is significantly more frequent after general anesthesia, and vomiting only occurs with general anesthesia. Local anesthesia was preferred by 94% of the participants (47 out of 50). The vast majority of patients in our study who have undergone both GA and LA DCR would choose LA again, providing a compelling case for use of the LA technique.
    Full-text · Article · Apr 2012 · Medical science monitor: international medical journal of experimental and clinical research
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    ABSTRACT: It is important to know the risk factors for visual field defect progression in order to predict the future development of glaucoma. To investigate the influence of visual field initial sensitivity on the rate of visual field progression. A minimum of five visual fields were performed using computerized perimetry (Octopus) over a mean followup 4-year period (48.85 +/- 17.84 months) in 64 persons (114 eyes) with primary open angle glaucoma. The rate of change of the whole central visual field and each of the seven regions of the field were measured by linear regression analysis of the mean sensitivity value versus time. Based on the slope of the regression line of visual field mean sensitivity over time the eyes were divided into two groups: the group with stable and the group with progressive visual field.The comparison of the mean sensitivity of the whole central visual field between the groups was performed using the Mann-Whitney U-test. The Spearman rang correlation coefficient and the Spearman probability p values were calculated to assess the mean sensitivity of the whole central visual field and of each visual field regions determined at the beginning of the study. The initial mean sensitivity of the whole central visual field was lower in the group with progressive visual field (16.62 +/- 6.35 dB). Correlation between the initial mean sensitivity and the slope of the regression line was negative. The lowest mean sensitivity was in the group with progressive visual field in the upper temporal (13.73 +/- 7.35 dB), nasal (14.74 +/- 7.23 dB) and upper nasal (15.03 +/- 7.75 dB) regions. The lower mean sensitivity value of the visual field, especially in the nasal and upper nasal visual field regions, carries a greater risk for delayed progressive loss of the visual field in persons with glaucoma.
    Preview · Article · Mar 2012 · Srpski arhiv za celokupno lekarstvo
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    ABSTRACT: Vascular dysregulation is deemed a significant risk factor in glaucoma occurrence and progression. Capillaroscopy of the blood vessels on the finger nail-fold is a method that can provide information regarding the state of the vascular system at the capillary level. The aim of this pilot study was to determine whether there are significant differences in the morphological characteristics of the peripheral blood vessels in normotensive glaucoma and primary open angle glaucoma. An ophthalmological and capillaroscopic examination was conducted on 30 normotensive glaucoma patients and 30 primary open angle glaucoma patients. The capillaroscopic characteristics described were as follows: capillary row density, capillary diameter, number of spirally formed capillaries, permeability of the loop, and loop resistance. Statistically, significantly more intensively spiraled capillaries were found in normotensive glaucoma patients (χ(2) test, p < 0.05). Results confirm the thesis that vascular factors play a significant role in the pathogenesis of the glaucoma, especially in cases where the level of intraocular pressure cannot be deemed responsible for the present damage of the optical nerve. Despite the newer, technologically more developed methods for diagnostics and monitoring glaucoma, it is often not easy to establish the right diagnosis and determine further the course of the illness, since the role the intraocular pressure (IOP) plays compared to the role of vascular factors is unknown; hence, capillaroscopy as a complementary diagnostic procedure can be of help.
    No preview · Article · Oct 2010 · Current eye research

Publication Stats

9 Citations
10.90 Total Impact Points

Institutions

  • 2010-2015
    • Klinički centar Srbije
      • Clinical Center of Serbia
      Beograd, Central Serbia, Serbia
  • 2012
    • University of Belgrade
      Beograd, Central Serbia, Serbia