Yieng-How Chen

Tunghai University, 臺中市, Taiwan, Taiwan

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Publications (10)11.11 Total impact

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    ABSTRACT: Ocimum gratissimum found in tropical regions is a traditional herb commonly which prevents free radical damage and protects liver from oxidative stress and fibrosis. Ocimum gratissimum polyphenol extract (OGPE) was purified by resin tube to 33.24% polyphenol and 8.2% flavonoid, which were three-fold higher compared with the pre-purification concentrations. The abstract was used to determine if the antioxidant components in the O. gratissimum extract (OGE) were responsible for protective effects on liver fibrosis. High-performance liquid chromatography analysis revealed that the content levels of catechin, caffeic acid and epicatechin in OGPE also increased three-fold. Male Wistar rats were administered with carbon tetrachloride (CCl₄) and varying amounts of OGPE doses [0-12 mg/kg body weight (BW)] or OGE dose (40 mg/kg BW) for 8 weeks. Results showed that OGPE at 12 mg/kg BW, similar to OGE at 40 mg/kg BW, maintained the liver weight, significantly ameliorated CCl₄-induced steatosis, and mitigated other pathological changes. OGPE (12 mg/kg BW) also maintained the levels of serum alanine aminotransferase and aspartate aminotransferase, as well as the levels of malondialdehyde, catalase and α-smooth muscle actin in liver tissues from CCl₄-induced changes. These findings suggest that antioxidant components in OGPE were the major factors that prevented liver fibrosis. Moreover, higher polyphenol concentrations were necessary for higher effectiveness.
    No preview · Article · Feb 2015 · The Chinese journal of physiology
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    ABSTRACT: Unlabelled: Aim/Materials and Methods: In order to develop better drugs against non-small cell lung cancer (NSCLC), we screened a variety of compounds and treated the human lung adenocarcinoma cell line A549 with different drug concentrations. We then examined the cell viability using the MTT assay. Results: Data show that a new candidate drug, acriflavine (ACF), suppresses the viability of A549 cells in a concentration- and time-dependent manner. Flow cytometry analysis revealed that ACF significantly caused cell growth arrest in the G2/M phase on A549 cells. Moreover, ACF decreased Bcl-2 expression and increased Bax expression. The content of cleaved poly(ADP-ribose)polymerase-1 (PARP-1) and caspase-3 are significantly increased. These findings suggest that ACF is cytotoxic against A549 cells and suppresses A549 cells growth through the caspase-3 activation pathway. In the in vivo test, nude mice bearing A549 cells xenografts by intravenous injection were randomly assigned into two groups: control and experimental group. Treatment was initiated 10 days after implantation and intraperitoneal injection of 0.9% normal saline or 2 mg/kg of ACF was continued daily for five weeks. ACF treatment significantly decreased tumor size and tumor spots on lung surface of tumor-bearing mice. Conclusion: ACF can inhibit cell growth in A549 cells. Our results may assist on the delineation of the mechanism(s) leading to NSCLC cell growth inhibition and provide a new antitumor strategy against NSCLC.
    No preview · Article · Nov 2014 · Anticancer research
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    ABSTRACT: Ocimum gratissimum (OG) was investigated in this study to determine its effect on the immune capability of black-feathered Taiwan country chickens. A total of 90 four-week-old male chickens were randomly assigned to a control group, which was fed with basal diet (BD), and two experimental groups, which were fed with a 0.2 and 0.4 g/kg OG-supplemented BD. During the experimental period, feed intake and body weight were recorded every two weeks to determine growth performance and feed efficiency. Blood was collected from the brachial vein of the chicken wing to obtain blood characteristics at 12 weeks of age. OG supplementation yielded no significant difference in growth performance and blood characteristics. The hemagglutination test showed that, compared with the control, the 0.4 g/kg BD group was able to maintain a significantly higher antibody titer level over two weeks after goat red blood cells injection (p<0.05), suggesting that 0.4 g/kg improves humoral immune response. The phytohemagglutinin test showed that wattle swelling in the 0.2-0.4 g/kg BD groups was reduced more significantly than that in the control group (p<0.05), suggesting that OG supplementation reduces cell-mediated immune response. Taken together, these findings suggest that although OG does not enhance growth or blood characteristics, the inverse changes in humoral and cell-mediated immune response may improve the overall health of the chickens.
    No preview · Article · Jul 2014 · The Journal of Poultry Science
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    ABSTRACT: Industrial wastewater containing heavy metals can become a serious environmental pollutant if not treated appropriately. Conventional treatment to remove toxic heavy metals can be expensive and may generate large amounts of toxic sludge. Regenerated natural wastes such as eggshells and eggshell membrane which are easily available, inexpensive, biodegradable, and have high adsorbability, can act as promising 'green' alternatives to remove heavy metal pollutants from wastewater. Here, we studied the adsorption capacity of eggshells with membrane (ESWM), eggshell membrane (ESM), and eggshells (ES) for the removal of nickel and silver ions in synthetic wastewater. Reaction time (1 to 72 h), metal ion concentration (25 to 200 mg/L), adsorbent dosage (0.1 to 0.8 g/20 mL), temperature (15 degrees C to 45 degrees C), and pH (1 to 9) were evaluated. Post-treatment nickel and silver concentrations were later analyzed using a spectrophotometer. Our results indicated increased removal of nickel and silver ions with increased adsorbent (all three ESWM, ESM, and ES) dosage, whereas the removal of nickel and silver ion decreased with increasing initial metal concentration. Among ESWM, ESM, and ES, ESM has the highest removal capacity and was the best adsorbent. The 0.8 g of ESM could remove 90.91% of nickel ions (100 mg/L) at 25 degrees C, pH 5.76 and 24 h. On the other hand, approximately 100% of silver ions (25 mg/L) could be removed by 0.2 g of ESM at 25 degrees C, pH 5.2, and 24 h. There was no difference in the adsorbability of ES and ESWM on nickel and silver ions. In summary, all three adsorbents, ESWM, ES, and ESM, can remove heavy metal ions from aqueous solution, with ESM having the highest efficiency. Hence, eggshell and its derivatives can be promising 'green' adsorbent materials for treating wastewater containing nickel and silver ions.
    No preview · Article · Mar 2014 · Food Science and Technology Research
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    Full-text · Article · Feb 2014 · Aerosol and Air Quality Research
  • Yieng-How Chen

    No preview · Article · Jan 2014 · Aerosol and Air Quality Research
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    ABSTRACT: In a recent study on hepatocellular carcinoma (HCC), we have shown that the transcription factors Myeloid Zinc Finger-1 (MZF-1) and Ets-like-protein 1 (Elk-1) are significantly related to protein kinase C alpha (PKCα) expression. The purpose of this study was to determine the correlation of the expression of PKCα with the expression of Elk-1 and MZF-1 in various differentiated urinary bladder transitional cell carcinoma (TCC) cell lines: 5637, BFTC905, TSGH8301, HT1376 and HT1197 cells. The malignant potential in the five TCC cell lines was examined by using cell proliferation/migration/invasion assay and the protein and mRNA levels of PKCα, ElK-1 and MZF-1 were examined by Western blot and RT-PCR analysis. The results showed that the rate of cell proliferation in the TSGH8301 cell line was higher than that in other cell lines, while there were obvious signs of cell migration and invasion in 5637, BFTC905 and HT1376 cells, and no sign in TSGH8301 and HT1197 cells. The resulting expression levels of Elk-1 and PKCα were the highest in 5637 cells, but the MZF-1 expression observed in all five cell lines showed no significant difference. To determine whether a correlation exists between PKCα and Elk-1, a shRNA knockout assay was performed and the results showed that the reduction of Elk-1 expression in 5637 cells did not result in the decreased PKCα expression. Therefore, although the findings showed elevated expression of Elk-1 and PKCα in 5637 cells, the regulator of PKCα in bladder cancer cells is yet to be determined.
    Full-text · Article · Apr 2012 · The Chinese journal of physiology
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    Yieng-How Chen · Shu-Yin Wang · Jenn-Chung Hsu
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    ABSTRACT: Three experiments were conducted to determine the conversion rate of formic and acetic acids into methane in the gastrointestinal tracts of geese. In experiment I, two sets of two 4-month-old male White Roman geese were allocated to one of two treatment groups. Each set of geese was inoculated either with formic acid or with phosphate buffer solution (PBS). After the acid or the PBS was inoculated into the esophagi of the geese, two birds from each treatment were placed in a respiratory chamber as a measurement unit for 4 h in order to determine methane production rate. In experiment II and III, 6- and 7-wk-old male White Roman goslings were used, respectively. Birds were allocated to receive either formic acid or PBS solution injected into the ceca in experiment II. Acetic acid or PBS solution injected into the cecum were used for experiment III. After either the acids or the PBS solution were injected into the cecum, two birds from each treatment were placed in a respiratory chamber as a measurement unit for 3 h; each treatment was repeated 3 times. The results indicated that formic acid inoculated into the oesophagi of geese was quickly converted into methane. Compared with the PBS-injected group, methane production increased by 5.02 times in the formic acid injected group (4.32 vs. 0.86 mg/kg BW/d; p
    Full-text · Article · Jul 2009 · Asian Australasian Journal of Animal Sciences
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    Yieng-How Chen · Shu-Yin Wang · Jenn-Chung Hsu
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    ABSTRACT: Two experiments of four-week duration were conducted to investigate the effect of caecectomy on the intestinal characteristics, body weight gain and gas production in the caeca of White Roman goslings. In experiment I, forty eight 2-wk-old female goslings with similar body weight were randomly divided into four treatments: sham (SHAM), left side caecum removed (LSCR), right side caecum removed (RSCR) and both caeca removed (CAECECTOMY). Smimilarly, experiment II was conducted with twelve 5-wkold male goslings in two treatments: SHAM and CAECECTOMY. Free choice water with ad libitum feed was provided during experiment. At the end of experiment I, goslings were sacrificed and gut length and weight were determined. At 7 and 9 wks of age, birds in experiment II were subjected to respiration calorimetry studies. In both experiments, final body weights were not affected by caecectomy. Results of experiment I indicated that caecectomy did not significantly affect the relative weight (g/100 g BW) of gizzard, small intestine, rectum and colon (p>0.05); however, the relative length of colon and rectum did increase (p
    Full-text · Article · Jul 2003 · Asian Australasian Journal of Animal Sciences
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    Yieng-How Chen · Hoang-Kao Hsu · Jenn-Chung Hsu
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    ABSTRACT: The objective of this study was to investigate the villus distribution in the caeca of domestic geese based on the fine structure. The caeca of White Roman geese, 14-week old, were sampled and specimens were detected under photomicroscope and scanning electron microscope (SEM). The results indicated that the villi existed at the proximal caecum. The morphologies of these villi showed finger-like, peak-like or tongue-like shapes. The heights of the villi decreased far from the proximal caecum. No villi were found in the middle and distal caecum. It was obvious that the proximal segment was the main portion for absorbing food nutrients in the caeca. The caecal content particles were small and possessed a viscid character. The large particles filtered out at the proximal caecum just like a mesh. The surface of the middle caecum exhibited parallel ridges with no villi. There were band plicae circular shapes found in the middle caecum under scanning electron microsopy.
    Preview · Article · Jul 2002 · Asian Australasian Journal of Animal Sciences

Publication Stats

22 Citations
11.11 Total Impact Points

Institutions

  • 2009-2015
    • Tunghai University
      • Department of Animal Science and Biotechnology
      臺中市, Taiwan, Taiwan
  • 2002
    • National Chung Hsing University
      • Department of Animal Science
      臺中市, Taiwan, Taiwan