[Show abstract][Hide abstract] ABSTRACT: In order to study the effect of inhalable particles on provocation of rat allergic rhinitis model and its possible mechanism.
After basic sensitizing the experimental rats with OVA by intraperitoneal injection, three groups were provocated by solo OVA, PM10+OVA and solo PM10 through nasal cavity respectively. The control group was sensitized by NS instead of OVA, and then provocated by NS as the method of experimental groups. The symptoms of rat during the provocation were observed and recorded. Then, the rats were sacrificed 24 hours after the last provocation, and the expression of IL-4 in nasal mucosa were detected by immunohistochemical method.
The groups of OVA and PM10+OVA both had provocated the obvious symptoms of allergic rhinitis, while the groups of PM10 and NS had the symptoms of scratching nose occasionally. By statistical analysis of the amount of IL-4 positive cells, there were significant differences between each experimental group and NS group, and there was significant difference between three experimental groups. The amount of IL-4 positive cells in PM10+OVA group was obviously higher than that in other groups.
In the period of provocation the rat allergic rhinitis, the inhalable particles play a synergic role with allergen, and it can aggravate the symptoms of allergic rhinitis.
No preview · Article · Jun 2011 · Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology
[Show abstract][Hide abstract] ABSTRACT: To study the mechanism of the nasal mucous membrane inflammation induced by the inhalable particle matter (PM10).
Three dosage PM10 were instilled in rat nasal cavity of different groups for one week. The morphology of nasal mucosa and the numbers of inflammatory cell were observed in each samples.
The total numbers of inflammatory cells in PM10-treated groups were increased in a dose-respondent manner and significantly different from that in control group. The results of histopathological and scanning electron microscope (SEM) analysis indicated that PM10 caused nasal mucosa injury and pathological changes, such as the damage of cilia and nasal mucosa epithelium in a dose dependent way. The infiltration of inflammatory cells in nasal mucosa epithelium matrix, especially eosinophilia were observed.
PM10 can cause rat's nasal mucosa inflammation and epithelial injury.
No preview · Article · Feb 2008 · Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology
[Show abstract][Hide abstract] ABSTRACT: To study the roles of leukotriene and histamine in the pathogenesis of allergic rhinitis and to compare the efficacy between leukotriene receptor antagonist and antihistamine by mensurating specific IgE in serum and eosinophil in peripheral blood and nose secretion of patients with allergic rhinitis respectively.
The patients were randomly divided into two groups and administrated montelukast sodium and loratadine respectively for two weeks. The specific IgE in serum and eosinophil in peripheral blood and nose secretion smear of patients were detected and then the state was evaluated by patients themselves and doctors.
After treatment the level of specific IgE in serum and eosinophil count in peripheral blood and nose secretion smear of patients were significantly lower than that before treatment (P < 0.05); There were no significant differences in the change of the level of specific IgE in serum and eosinophil count in peripheral blood and nose secretion smear between two groups after treatment (P > 0.05).
Treating patients with allergic rhinitis with montelukast sodium and loratadine were of similar effect. Histamine and leukotriene are both important inflammatory factors in the pathogenesis of allergic rhinitis and both play roles by affecting eosinophil accumulation and activation and by decreasing the formation of specific IgE. Leukotriene receptor antagonist is a new drug to treat allergic rhinitis.
No preview · Article · May 2005 · Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology