Publications (18)

Publications citing this author (75)

    • The pattern in CCH cells was fibroblastic and myoctyic, but fibroblastic cells were dominant after subsequent passage. Most of the primary cultures reported from fish in India were comprised of fibroblastic cells (Singh et al., 1995; Lakra & Bhonde1996). A predominance of fibroblastic cells over epithelioid cells in cell cultures from fish has been reported by several workers in the past (Bejar et al., 1997; Chi et al., 1999).
    [Show abstract] [Hide abstract] ABSTRACT: Two new cell lines (CCF and CCH) were established from fin and heart tissues of common carp, Cyprinus carpio. The cells were optimally maintained in Leibovitz-15 medium supplemented with 10% fetal bovine serum (FBS) and 10 ng/ml of basic fibroblastic growth factor (bFGF). The effects of temperature, concentration of FBS and bFGF on the growth of CCF and CCH cells were examined. The temperature ranged from 24 to 32°C for good growth of the cells. The growth rate of cells was higher in medium containing 10% FBS and the addition of bFGF to the medium significantly increased the growth rate. The CCF cells were found to be epithelial, while the CCH cells were fibroblastic in nature. The cytogenetic analysis of the cell lines revealed a diploid number of 100 chromosomes in C. carpio. The viability of CCF and CCH cell lines were 70 and 72%, respectively, after six months of storage in liquid nitrogen (-196° C). Molecular characterization of the cell lines using 16S rRNA and Cytochrome Oxidase Subunit I (COI) revealed the origin of the cell lines. These new cell lines will be useful for isolation of fish viruses and other in vitro biotechnological studies.
    Full-text · Article · Jan 2010
    • Myopericarditis is an uncommon medical condition characterized by symptoms and signs of pericarditis with myocardial involvement. It could be associated with infectious etiologies, mainly viral, like adenovirus [1], echovirus [2] and coxsackie virus [3]. It was described also following streptococcal infection in few cases [4][5][6][7][8].
    Article · Jan 2014
    • In the present study, positivity for HBV (2.4 %) was the highest. This is in agreement with the 2.6 % carrier rate of HBsAg reported in a follow-up study of asymptomatic mothers from South India [4]. In a recent study from Delhi, 4.25 % mothers were found to be carriers [5].
    [Show abstract] [Hide abstract] ABSTRACT: To determine the current prevalence of four major sexually transmitted infections (STIs: HIV, Hepatitis B, Herpes simplex virus 2, and Syphilis) in asymptomatic pregnant women. This is a prospective study of 500 consecutive, apparently healthy asymptomatic pregnant women who were attending the antenatal clinic. The information regarding their socio-demographic and behavioral characteristics and obstetric performance was recorded. The blood samples was collected after obtaining their informed written consent from those who were tested for the HIV antibodies (NACO guidelines), HBsAg (ELISA test), HSV2-IgM (ELISA test), and Syphilis (VDRL and TPHA tests). The overall prevalence of one or the other four STIs studied was 4.8 % with the highest prevalence of HBV (2.4 %), followed by HSV-2 (2 %), and HIV (0.4 %). No woman tested positive for syphilis and multiple infections. All the infections were more common in illiterate, multigravida, monogamous women of low socio-economic status. High-risk sexual behavior of the husbands, history of STIs in husbands, and blood transfusions were the other factors associated with the prevalence of these infections. The relatively high prevalence of HBV and HSV-2 infections in asymptomatic pregnant women suggests that there is need of screening for HBV and HSV-2 infections along with the pre-existing screening for HIV and Syphilis and universal immunization of HBV high-risk infants.
    Article · Apr 2012
    • So, this study revealed that immunochromatographic method is less reliable for the screening of HBsAg. The study of Shanmugam et al, (1983) clearly showed that ELISA is the most sensitive technique. So, more sensitive test like ELISA should be used to screen donors to prevent transmission of hepatitis to patients and the hospital personnel (Kuhns and Busch, 2006).
    [Show abstract] [Hide abstract] ABSTRACT: The present study was a cross sectional descriptive study to check the prevalence of Hepatitis B surface Antigen (HBsAg) among pediatric patients which included a total of 2,152 children both male and female who reported to Department of Immunology in Children's Hospital Lahore, from September 2004 to August 2005. The prevalence of HBsAg among the pediatric patients was found to be 5.1%, during the specified course of study. Further, the detailed history of 109 HBsAg reactive subjects was collected to assess the prevalence of HBsAg in different age and sex groups, the distribution of different signs and symptoms among HBsAg positive patients, risk factors among HBsAg positive patients and the prevalence of HBsAg on the basis of socioeconomic status. Finally, to evaluate the immunochromatographic method (Bioline), using Enzyme Linked Immunosorbent Assay (ELISA, Biorad) as golden standard for the detection of HBsAg, a sub-cohort of 200 subjects was analyzed. The specificity and sensitivity of the immunochromatographic method was found to be 99.5% and 84.6% respectively. From this evaluation it was concluded that immunochromatographic technique is less reliable for screening than the ELISA.
    Full-text · Article · Jan 2008
    • Frankreich [52], 62,5 % in Schweden [35], 62 % in den USA [89], 85,4 % in Indien [144], 87,0 % in Singapur [166] und 97 % in Jamaika [118] % bei Schwangeren weißer Hautfarbe, 88,2 % bei Asiatinnen und 77,2 % bei Schwangeren dunkler Hautfarbe [154]. Die CMV-IgM-Seroprävalenzraten liegen zwischen 0 % in Schweden [35] und 8,5 % in Indien [121] bzw.
    [Show abstract] [Hide abstract] ABSTRACT: Mit der Entdeckung des Humanen Herpesvirus Typ 8 (HHV-8) und seiner Rolle in der Entstehung des Kaposi Sarkom und anderen Erkrankungen gewann auch die Frage nach den Übertragungswegen des Virus eine große Bedeutung. Vor allem die Möglichkeit der vertikalen Übertragung wird seitdem intensiv und kontrovers diskutiert. Um die Frage nach der Möglichkeit einer vertikalen (intrauterinen) Übertragung des HHV-8 zu beantworten, wurden zunächst 308 Proben mütterlichen Blutes aus der Eurocord Bank der Universität Düsseldorf auf das Vorhandensein von IgG-Antikörper gegen HHV-8 untersucht. Das Blut der für positiv befundeten Mütter (46/308, 14,9 %) wurde auf IgM-Antikörper gegen HHV-8, auf IgG- und IgM-Antikörper gegen CMV sowie auf HHV-8-DNA getestet und das der Neugeborenen (46) auf IgG- und IgM-Antikörper gegen HHV-8 sowie HHV-8-DNA. IgM-Antikörper gegen HHV-8 wurden bei 10 (21,7 %), HHV-8-DNA bei 2 (4,3 %), IgG-Antikörper gegen CMV bei 29 (63,0 %) und IgM-Antikörper gegen CMV bei 2 (4,3 %) der 46 Mütter gefunden. Von den 46 Neugeborenen wiesen 41 (89,1 %) IgG-Antikörper gegen HHV-8 und keines IgM-Antikörper gegen HHV-8 auf. Bei einem Neugeborenen (2,2 %) konnte mittels nested-PCR HHV-8-DNA im Nabelschnurblut nachgewiesen werden, ein Beweis für das Auftreten einer Infektion in utero.
    Article · Indian Journal of Fisheries
    • These cell lines are being used for isolation and characterization of previously unknown viruses of this region. In India, there has been quite a progress towards the development of cell lines from Indian major carps and other warm water fishes (Singh et al., 1995, Lakra and Bhonde, 1996; Rao et al., 1997; Kumar et al., 1998; Prassanna et al., 2000; KAnar et al., 2001, Rathore et al., 2001). A few cell lines MG-3 (Sathe et al., 1995) and RG-I and RG-2 (Sathe et al., 1997) derived from gills of Cirrhinus mrigala and Labeo rohita, respectively have been established and characterized.
    [Show abstract] [Hide abstract] ABSTRACT: The study reports a successful development of primary cell culture from fin explants of labeo rohita and subsequent subculture up to eight passages. Explant tissues of fin were seeded and incubated at 30°C in DMEM supplemented with 10% FCS. On day 5 of seeding, the differentiation of the cells was visible and a complete monolayer was obtained on day 20. A cell count of 2 X 10 6 cells was sufficient to produce monolayer in 5 days on 8 th passage. These encouraging results are indicative of a high probability of developing a cell line from the fin tissue of L. rohita.
    Full-text · Article · Feb 2007
    • Results from this study showed HBsAg positivity prevalence being 2.06 times more among males than the females. Available reports show that men are more likely to test positive for HBsAg than women[13]. This trend is not dependent on both the prevailing risk factors and the level of endemicity in any randomly sampled population.
    [Show abstract] [Hide abstract] ABSTRACT: Ghana is not an exception from developing countries suffering from hepatitis B infection. Research has shown that various communities have different prevalent rate as result of life style and socioeconomic levels. This study investigated the prevalence of the infection, possible risk factors, determined liver enzymes (AST and ALT) of infected respondents and serologically characterized the infected respondents in KNUST community. In a total of 85 respondents, 8 of them tested positive to the HBsAg indicating 9.41% prevalence. Males have higher prevalence (13.16%) than females (6.38%). Ages from 20-29 recorded the highest prevalence followed by 30-39 and 50-59. The mean values obtained for AST in U/l was (40.33±13.60) and ALT in U/l was (25.17±5.70). The mean values indicate normal health for liver cells. Three of the respondents reported of having a family history of HB virus, two claimed they had been blood-transfused and one was involved in sharing of devices. Therefore, age, sex, history of blood transfusion, use of shared items and blood contacts have much influence and could be major contributing factors to HBV infection. Serological evidence indicates that positive respondents to HBsAg fall within the inactive chronic hepatitis B carrier phase and so it can be concluded that the chronic hepatitis B-infected patients visiting the Clinical Analysis Laboratory are in the inactive phase.
    Full-text · Article · Apr 2017 · Indian Journal of Fisheries