Y.-J. Liu

Food Industry Research and Development Institute, Hsin-chu-hsien, Taiwan, Taiwan

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Publications (2)0.45 Total impact

  • H.-H. Shen · T.-Y. Su · Y.-J. Liu · H.-Y. Chang · D.-J. Yao
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    ABSTRACT: To use efficiently a digital microfluidic system with an electrowetting on a dielectric (EWOD) technique to detect a single-nucleotide polymorphism SNP in a specific gene sequence, we designed microheaters on the cover of an EWOD chip for temperature control of a bioreaction. The droplets on an EWOD chip were actuated with addressable electrodes. Multiple bioreagents such as DNA, micromagnetic beads, and buffer droplets have been manipulated using the EWOD system. Magnetic beads (MBs), whose magnetic property enabled them to be collected and purified with an applied magnetic field, were used to detect an SNP in a specific gene sequence. The microheaters were fabricated on a glass substrate with Pt deposition. After designing the heater circuit, the top plate was constructed with a bottom chip to complete the packaging of a temperature-controllable EWOD system. These microheaters in an EWOD system can provide an appropriate temperature for DNA ligase in experiments to detect an SNP. The temperature-controllable EWOD digital microfluidic system detected an SNP code in a specific gene sequence. This research will contribute to biomedical diagnosis through a lab-on-a-chip approach.
    No preview · Article · Jan 2013 · Sensors and Materials
  • H.C. Lin · Y.J. Liu · D.J. Yao · H.Y. Chang
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    ABSTRACT: This paper reports the completion of a parallel ultramicro-volume DNA ligation processes using a coplanar electrode type of electrowetting-on-dielectric (EWOD) microfluidic system. The droplets, containing DNA, a ligation enzyme and a multi-salt reaction buffer, served to complete the ligation using a developed EWOD system. In order to reduce the effect from environment, so the concept of core-shell droplet was used in this study. Droplets of ultramicro volume (0.3 muL) were successfully generated from reservoirs between one plate with coplanar electrodes and another plate with a hydrophobic surface free of electrodes. In one successful cloning, the total usage of reagents in an ultramicro-volume EWOD chip was 2.1 muL; no volume was wasted, in comparison with 85% waste with the standard protocol (15 muL). The results also show that the four parallel DNA ligation processes were accomplished without damage to the chip surface and without biomaterial annulment.
    No preview · Conference Paper · Jul 2009

Publication Stats

3 Citations
0.45 Total Impact Points

Top Journals


  • 2013
    • Food Industry Research and Development Institute
      Hsin-chu-hsien, Taiwan, Taiwan
  • 2009
    • National Tsing Hua University
      • Institute of NanoEngineering and MicroSystems
      Hsin-chu-hsien, Taiwan, Taiwan