Congiu Castellano A

Sapienza University of Rome, Roma, Latium, Italy

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Publications (2)0 Total impact

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    ABSTRACT: The aim of this research is to investigate some bioeffects due to Therapeutic Ultrasound (1 MHz and 50<IPA<60 W/cm2) which could allow to enhance drugs or genes delivery in non tumoral cells. Ultrasound (US) has been demonstrated to alter the cell membrane permeability due to a biophysical mechanism, Sonoporation, and exploited as a promising non-invasive gene transfer method. We have used the NIH-3T3 cell line as a model system and exposed it to US medical equipment for 15, 30, 45, 60 minutes at distances of 10 and 15 cm from the source transducer, corresponding to the far field region where cm. We have worked with the maximum power in pulsed system with 75% duty cycle. Characterization of the unfocused, planar and with a circular geometry 1 MHz source transducer, was performed and the acoustics pressure was measured by a calibrated 0.5 mm needle hydrophone; moreover, the pressure field generated by the source transducer was simulated. The US effects on cells were assessed by Fourier transform infrared (FTIR) Imaging with focal plane array (FPA) detector. By the IR analysis, the US exposure on non tumoral cells has induced a change of the intensity for CH2 asymmetric stretching (2924 cm−1) band in the lipid region (3000-2800 cm−1) that it could detect an energy-dependent process. It has already shown that cells invest energy to catalyze lipid movement in order to maintain a specific transmembrane phospholipid distribution. Although asymmetry is the rule for control cells, the loss of asymmetry could be associated with the permeability change of plasma membrane inducing temporary pores.
    No preview · Article · Feb 2011 · Journal of Physics Conference Series
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    ABSTRACT: Actually ultrasound irradiation is extensively used in biomedical fields, such as diagnosis, physiotherapy and cancer therapy. It is well known that ultrasound induces in the organic and inorganic matter, chemical and physical effects which are dependent on the ultrasound frequency and the acoustic intensity. Nevertheless contradictory results have been reported concerning the influence of ultrasound on enzyme inactivation, proteins structure, cell apoptosis [1-4]. We focused our attention on the effects induced on cell lines by therapeutic ultrasound at 1 MHz frequency used in physiotherapy to treat different pathologies. We investigated the structural changes due to ultrasound in two cell lines ( Jurkat T lymphocytes and murine fibroblasts NIH 3T3) by using FTIR Spectroscopy, optical and fluorescence microscopy coordinated with the micronuclei test to reveal genotoxic and cytotoxic damages [5-9]. Infrared spectroscopy is a powerful technique able to reveal conformational changes in functional groups of biological samples; the fingerprint region of IR spectra of living cells can detect changes in lipids, nucleic acids and secondary structure of cell proteins. The micronuclei test � is a cytogenetic assay consisting in the detection of the variations of micronucleated cells frequencies.
    No preview · Conference Paper · Jun 2009