Hang Wang

Wuhan General Hospital of Guangzhou Military Command, Wu-han-shih, Hubei, China

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Publications (128)228.75 Total impact

  • [Show abstract] [Hide abstract] ABSTRACT: Purpose: To investigate the influence of surface damage on the fatigue behavior of zirconia under two different loading conditions. Materials and methods: One hundred twenty zirconia bar-shaped received either airborne particle abrasion using 50μm or 120μm alumina particles while polished specimens served as control. The specimens were subjected to two fatigue regimes: dynamic fatigue (1,000,000 cycles, 1hz and 0.5s contact time) or static fatigue (a constant load applied for 5000s) under water using the staircase application of the load. The flexure strength after fatigue (dynamic fatigue strength) was compared to the initial flexure strength of the tested specimens (α=0.05). The critical crack shape and size of fractured specimens was examined using scanning electron microscopy. Results: Compared to the initial flexure strength of the tested specimens, dynamic fatigue strength was 86.3% for the polished specimens, 73.4% for 50μm particle abrasion, and 42.3% for 120μm particle abrasion while the static fatigue strength was 85.9%, 78.5%, and 51.5% respectively. Significant statistical differences (F=223.679, P<0.001) were found between different surface treatments but not between dynamic and static fatigue strengths for the same type of surface treatment. Conclusions: The dynamic and static fatigue strengths of zirconia are significantly influenced by type of surface damage. Clinical implications: Within the limitations of this study, surface damage have great influence on fatigue behavior of zirconia.
    No preview · Article · Apr 2016 · Dental materials: official publication of the Academy of Dental Materials
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    [Show abstract] [Hide abstract] ABSTRACT: The aim was to assess the efficacy and safety of articaine compared with lidocaine for irreversible pulpitis (IP) treatment. Databases were explored electronically and relevant journals as well as the references of the included studies were hand-searched for randomised clinical trials comparing the efficacy and safety of articaine with lidocaine in treatment of IP. Twenty studies were included, of which eight had low risk of bias, 10 had moderate risk of bias and two had high risk of bias. In comparison with 2% lidocaine with 1:100000 epinephrine, 4% articaine with 1:100000 epinephrine showed a higher success rate in anaesthesia of IP at either person (risk ratio (RR) 1.15; 95% confidence intervals (CI) 1.10 1.20; P<0.00001) or tooth unit (RR 1.10; 95% CI 1.10 1.19, P<0.00001), lower VAS scores during injection phase (mean difference (MD) -0.67; 95% CI -1.26 -0.08, P=0.02) and treatment phase (MD -3.35; 95% CI -3.78 -2.91, P<0.00001), shorter onset time of pulpal anaesthesia (MD -0.94; 95% CI -1.13 -0.74, P<0.00001) and lower percentage of patients undergoing adverse events (RR 0.17; 95% CI 0.03 0.92, P=0.04). Given the efficacy and safety of the two solutions, 4% articaine with 1:100000 epinephrine was superior to 2% lidocaine with 1:100000 epinephrine in dental treatments in IP.
    Full-text · Article · Apr 2016 · Australian Endodontic Journal
  • [Show abstract] [Hide abstract] ABSTRACT: This paper analyzes the task-oriented design method of six-axis force sensor and proposes the task model of the sensor. The task mathematical model of the sensor is established based on the idea of task ellipsoid. The models of force ellipsoid and moment ellipsoid are also established. The relational expression between the task model and ellipsoid model of sensor is obtained. Then, a fully pre-stressed dual-layer parallel six-axis wrist force sensor is proposed, whose static mathematical model is also established. The sensor task model for assembly work is proposed and the analytical expression between the sensor structure parameters and task model is deduced. According to the assembly work, the sensor structure is designed specifically, and the specific structure sizes of the sensor are obtained. Then the new sensor prototype manufactured for peg-in-hole assembly is processed. The calibration experiment and peg-in-hole assembly experiment on the prototype are completed and each performance index is well examined by the experiment results. The experiment results also lay the foundation for the practical application of six-axis force sensor.
    No preview · Article · Feb 2016
  • [Show abstract] [Hide abstract] ABSTRACT: In order to reduce shrinkage and improve the mechanical properties of dental composite resins, we designed a hybrid resin formulation containing a novel matrix resin, BisS-GMA [bisphenol-Sbis( 3-methacrylato-2-hydroxypropyl)ether], and epoxy modified by a spiro-orthocarbonate (SOC) expanding monomer. Then, we tested the effects of an iodonium salt, diphenyliodonium hexafluorphosphate (DPIHFP), on the properties of the hybrid resin with seven different concentrations. The hybrid resin was polymerized by a ternary photo-initiator system. The volumetric shrinkage (VS), degree of conversion (DC) and compressive strength (CS) were assessed using AcuVol™, FTIR and a universal testing machine, respectively. The VS, DC and CS were improved with increasing DPIHFP concentration, but a high concentration of DPIHFP had a negative influence on the mechanical properties of the hybrid resin and offered no added improvement in the VS and DC. The best performance of a composite resin containing BisS-GMA and SOC-modified epoxy was achieved with 2wt% DPIHFP. The results also indicated that the resin containing BisS-GMA was superior to that containing Bis-GMA in terms of VS, DC and CS.
    No preview · Article · Dec 2015 · Journal of Wuhan University of Technology-Mater Sci Ed
  • [Show abstract] [Hide abstract] ABSTRACT: This study investigated the microhardness and fracture toughness values of five dental indirect resin composites under the effect of thermal cycling by single-edge notched beam method. Highest microhardness and fracture toughness were reached in AP-X and Filtek P60 groups (microhybrid resin composite) (P < 0.05). After thermal cycling, significant changes of fracture toughness were found for the tested composites except AP-X. Microdifferences between scanning electron microscope images with and without thermal cycling were observed. It is concluded that irregular-shaped fillers and higher filler content contribute to higher mechanical properties, microhardness, and fracture toughness and result in superior fracture toughness under the effect of thermal cycling.
    No preview · Article · Nov 2015 · Particulate Science And Technology
  • [Show abstract] [Hide abstract] ABSTRACT: To investigate the effects of chilling and partial freezing on rigor mortis changes in bighead carp (Aristichthys nobilis), pH, cathepsin B, cathepsin B+L activities, SDS-PAGE of sarcoplasmic and myofibrillar proteins, texture, and changes in microstructure of fillets at 4 °C and -3 °C were determined at 0, 2, 4, 8, 12, 24, 48, and 72 h after slaughter. The results indicated that pH of fillets (6.50 to 6.80) was appropriate for cathepsin function during the rigor mortis. For fillets that were chilled and partially frozen, the cathepsin activity in lysosome increased consistently during the first 12 h, followed by a decrease from the 12 to 24 h, which paralleled an increase in activity in heavy mitochondria, myofibrils and sarcoplasm. There was no significant difference in cathepsin activity in lysosomes between fillets at 4 °C and -3 °C (P > 0.05). Partially frozen fillets had greater cathepsin activity in heavy mitochondria than chilled samples from the 48 to 72 h. In addition, partially frozen fillets showed higher cathepsin activity in sarcoplasm and lower cathepsin activity in myofibrils compared with chilled fillets. Correspondingly, we observed degradation of α-actinin (105 kDa) by cathepsin L in chilled fillets and degradation of creatine kinase (41 kDa) by cathepsin B in partially frozen fillets during the rigor mortis. The decline of hardness for both fillets might be attributed to the accumulation of cathepsin in myofibrils from the 8 to 24 h. The lower cathepsin activity in myofibrils for fillets that were partially frozen might induce a more intact cytoskeletal structure than fillets that were chilled.
    No preview · Article · Nov 2015 · Journal of Food Science
  • No preview · Article · Oct 2015 · Journal of the American College of Cardiology
  • Hang Wang · Ke-Yin Cai · Wei Li · Hao Huang
    [Show abstract] [Hide abstract] ABSTRACT: Endothelial progenitor cells (EPCs) play a fundamental role in neoangiogenesis and tumor angiogenesis. Through the sphingosine-1-phosphate receptor 3 (S1PR3), sphingosine-1-phosphate (S1P) can stimulate the functional capacity of EPCs. Platelet-derived growth factor receptor-beta (PDGFR-β) contributes to the migration and angiogenesis of EPCs. This study aimed to investigate whether S1P induces the migration and angiogenesis of EPCs through the S1PR3/PDGFR-β/Akt signaling pathway. We used the Transwell system and the Chemicon In Vitro Angiogenesis Assay Kit with CAY10444 (an S1PR3 antagonist), AG1295 (a PDGFR kinase inhibitor) and sc-221226 (an Akt inhibitor) to examine the role of the S1PR3/PDGFR-β/Akt pathway in the S1P -induced migration and angiogenesis of EPCs.
    No preview · Article · Jul 2015 · Cellular & Molecular Biology Letters
  • [Show abstract] [Hide abstract] ABSTRACT: Silent information regulator 1 (SIRT1) mediates many effects of caloric restriction (CR) on an organism's lifespan and metabolic pathways. Recent reports have also emphasized its role in vascular function. The present study was designed to investigate the effects of SIRT1 on the properties of mouse spleen derived endothelial progenitor cells (EPCs). SIRT1 in EPCs was significantly increased by serum and by vascular endothelial growth factor (VEGF). Moreover, an adenovirus (Ad) vector expressing SIRT1 (Ad-SIRT1)-mediated overexpression of SIRT1 directly enhanced migration and proliferation of EPCs, whereas silencing of endogenous SIRT1 in EPCs inhibited cell functions. In addition, LY294002 (a PI3K inhibitor), sc-221226 (an Akt inhibitor), and L-NAME (an NOS inhibitor) abolished Ad-SIRT1-induced migration and proliferation of EPCs, and prevented nitric oxide (NO) production. Phosphorylation of Akt, PI3K, and endothelial nitricoxide synthase (eNOS) were up-regulated by Ad-SIRT1, which was attenuated by LY294002, sc-221226, and L-NAME. Together, the results suggested that through the PI3K/Akt/eNOS signaling pathway, SIRT1 plays an important role in the biological properties of EPCs.
    No preview · Article · Jun 2015 · International journal of clinical and experimental pathology
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    [Show abstract] [Hide abstract] ABSTRACT: To measure the surface loss of dental restorative zirconia and the short-term bond strength between an indirect composite resin (ICR) and zirconia ceramic after various sandblasting processes. Three hundred zirconia bars were randomly divided into 25 groups according to the type of sandblasting performed with pressures of 0.1, 0.2, 0.4 and 0.6 MPa, sandblasting times of 7, 14 and 21 seconds, and alumina powder sizes of 50 and 110 µm. The control group did not receive sandblasting. The volume loss and height loss on zirconia surface after sandblasting and the shear bond strength (SBS) between the sandblasted zirconia and ICR after 24-h immersion were measured for each group using multivariate analysis of variance (ANOVA) and Least Significance Difference (LSD) test (α=.05). After sandblasting, the failure modes of the ICR/zirconia surfaces were observed using scanning electron microscopy. The volume loss and height loss were increased with higher sandblasting pressure and longer sandblasting treatment, but they decreased with larger powder size. SBS was significantly increased by increasing the sandblasting time from 7 seconds to 14 seconds and from 14 seconds to 21 seconds, as well as increasing the size of alumina powder from 50 µm to 110 µm. SBS was significantly increased from 0.1 MPa to 0.2 MPa according to the size of alumina powder. However, the SBSs were not significantly different with the sandblasting pressure of 0.2, 0.4 and 0.6 MPa. The possibilities of the combination of both adhesive failure and cohesive failure within the ICR were higher with the increases in bonding strength. Based on the findings of this study, sandblasting with alumina particles at 0.2 MPa, 21 seconds and the powder size of 110 µm is recommended for dental applications to improve the bonding between zirconia core and ICR.
    Preview · Article · Jun 2015 · The journal of advanced prosthodontics
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    Qi Chen · Lufei Wang · Lina Ge · Yuan Gao · Hang Wang
    [Show abstract] [Hide abstract] ABSTRACT: Purpose: To assess the efficacy of midazolam for anxiety control in third molar extraction surgery. Methods: Electronic retrievals were conducted in Medline (via PubMed, 1950-2013.12), the Cochrane Central Register of Controlled Trials (CENTRAL) (The Cochrane Library 2013, Issue 3), Embase (via OVID 1974-2013.12), and the System for Information on Grey Literature in Europe (SIGLE). The bibliographies of relevant clinical trials were also checked. Randomized controlled trials satisfying the inclusion criteria were evaluated, with data extraction done independently by two well-trained investigators. Disagreements were resolved by discussion or by consultation with a third member of the review team. Results: Ten studies were included, but meta-analysis could not be conducted because of the significant differences among articles. All but one article demonstrated that midazolam could relieve anxiety. One article demonstrated that propofol offered superior anxiolysis, with more rapid recovery than with midazolam. Compared with lorazepam and diazepam, midazolam did not distinctly dominate in its sedative effect, but was safer. Two articles used midazolam in multidrug intravenous sedation and proved it to be more effective than midazolam alone. Conclusion: It was found, by comparison and analysis, that midazolam might be effective for use for anxiety control during third molar extraction and can be safely administered by a dedicated staff member. It can also be used with other drugs to obtain better sedative effects, but the patient's respiratory function must be monitored closely, because multidrug sedation is also more risky.
    Preview · Article · Apr 2015 · PLoS ONE
  • Hang Wang · Bona Dai · Bin Liu · Han Lu
    [Show abstract] [Hide abstract] ABSTRACT: Hydrophobic compounds with hydroxyl, aldehyde or ketone groups are generally difficult to detect using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), because these compounds have low proton affinity and are poorly ionized by MALDI. Herein, coumarins have been used as new matrices for MALDI-MS analysis of a variety of hydrophobic compounds with low ionization efficiency, including steroids, coenzyme Q10, a cyclic lipopeptide and cholesterol oleate. Five coumarins, including coumarin, umbelliferone, esculetin, 7-hydroxycoumarin-3-carboxylic acid (HCA) and 6,7-dihydroxycoumarin-3-carboxylic acid (DCA), were compared with the conventional matrices of 2,5-dihydroxybenzoic acid (DHB) and α-cyano-4-hydroxycinnamic acid (CHCA). Coumarins with hydroxyl or carboxylic acid groups enabled detection. Taking DCA as an example, this matrix proved to be superior to DHB or CHCA in detection sensitivity, stability, spot-to-spot and sample-to-sample reproducibility, and accuracy. DCA increased the stability of the target compounds and decreased the loss of water. The [M+Na](+) peaks were observed for all target compounds by adding NaCl as an additive, and the [M-H2O+H](+) and [M+H](+) peaks decreased. DCA was selected for the identification of sterols in yeast cells, and thirteen sterols were detected by Fourier transform ion cyclotron resonance (FT ICR) mass spectrometry. This work demonstrates the potential of DCA as a new matrix for detection of hydrophobic molecules by MALDI-MS and provides an alternative tool for screening sterols in antifungal research. Copyright © 2015 Elsevier B.V. All rights reserved.
    No preview · Article · Apr 2015 · Analytica Chimica Acta
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    Feng Li · Weihua Guo · Kun Li · Mei Yu · Wei Tang · Hang Wang · Weidong Tian
    [Show abstract] [Hide abstract] ABSTRACT: The success of soft-tissue augmentation is offset by the low survival rates of grafted fat tissue. Research shows that adipose-derived stem cells (ASCs) and platelet-rich plasma (PRP) are beneficial to tissue healing. To evaluate the long-term effects of different volume fractions of PRP combined with ASCs on fat graft. ASCs were isolated from human fat tissue, and PRP was obtained from human blood. Cell count kit-8 and real-time polymerase chain reaction (PCR) were used to evaluate the influence of PRP (0%, 10%, 20%, and 30%; volume/volume [v/v]) in medium on ASC proliferation and adipogenic differentiation, respectively. A novel lipoinjection consisting of granular fat, PRP, and ASCs was subcutaneously transplanted into nude mice. The grafts were volumetrically and histologically evaluated 10, 30, 60, and 90 days after transplantation. The addition of PRP improved ASC proliferation. Expression of adipogenic-related genes, peroxisome proliferator-activated receptor-γ, lipoprotein lipase, and adipophilin were up-regulated in PRP-induced ASCs. Compared with other groups, granular fat grafts formed with 20% (v/v) and 30% (v/v) PRP significantly improved residual volumes. More intact adipocytes and capillary formation, but less vacuolization, were observed in the 20% (v/v) and 30% (v/v) PRP groups at 30, 60, and 90 days. However, no significant difference was observed between the 20% (v/v) and 30% (v/v) PRP groups in retaining fat grafts and improving histology. Fat grafting with 20% (v/v) PRP and ASCs constitutes an appropriate transplantation strategy for improving graft survival and provides a potential approach for soft-tissue restoration in plastic and reconstructive surgery. © 2015 The American Society for Aesthetic Plastic Surgery, Inc. Reprints and permission: journals.permissions@oup.com.
    Full-text · Article · Mar 2015 · Aesthetic surgery journal / the American Society for Aesthetic Plastic surgery
  • [Show abstract] [Hide abstract] ABSTRACT: 5-Hydroxytryptophan (5-HTP) is the precursor of serotonin and 5-HTP has been widely used as a dietary supplement to raise serotonin level. Serotonin has recently been discovered to be a novel and important player in bone metabolism. As peripheral serotonin negatively regulates bone, the regular take of 5-HTP may affect the alveolar bone metabolism and therefore influence the alveolar bone loss induced by periodontitis. The aim of this study was to investigate the effect of 5-HTP on alveolar bone destruction in periodontitis. Male Sprague-Dawley rats were randomly divided into the following four groups: (1) the control group (without ligature); (2) the 5-HTP group (5-HTP at 25mg/kg/day without ligature); (3) the L group (ligature+saline placebo); and (4) the L+5-HTP group (ligature+5-HTP at 25mg/kg/day). Serum serotonin levels were determined by ELISA. The alveolar bones were evaluated with micro-computed tomography and histology. Tartrate-resistant acid phosphatase staining was used to assess osteoclastogenesis. The receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG) expression in the periodontium as well as the interleukin-6 positive osteocytes were analysed immunohistochemically. 5-HTP significantly increased serum serotonin levels. In rats with experimental periodontitis, 5-HTP increased alveolar bone resorption and worsened the micro-structural destruction of the alveolar bone. 5-HTP also stimulated osteoclastogenesis and increased RANKL/OPG ratio and the number of IL-6 positive osteocytes. However, 5-HTP treatment alone did not cause alveolar bone loss in healthy rats. The present study showed that 5-HTP aggravated alveolar bone loss, deteriorated alveolar bone micro-structure in the presence of periodontitis, which suggests 5-HTP administration may increase the severity of periodontitis. Copyright © 2015 Elsevier Ltd. All rights reserved.
    No preview · Article · Feb 2015 · Archives of Oral Biology
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    [Show abstract] [Hide abstract] ABSTRACT: Derived from mature adipocytes, dedifferentiated fat (DFAT) cells represent a special group of multipotent cells. However, their phenotype and cellular nature remain unclear. Our study found that human DFAT cells adopted perivascular characteristics and behaviors. Flow cytometry and immunofluorescent staining revealed that human DFAT cells positively expressed markers highly related to perivascular cell lineages, such as CD140b, NG2 and desmin, but were negative for common endothelial markers, including CD31, CD34, and CD309. Furthermore, DFAT cells displayed vascular network formation ability in Matrigel, and they noticeably promoted and stabilized the vessel structures formed by human umbilical vascular endothelial cells (HUVECs) in vitro. These results provide novel evidence on the pericyte nature of human DFAT cells, further supporting the recent model for the perivascular origin of adult stem cells, in which tissue-specific progenitor cells in mesenchymal tissues associate with blood vessels, exhibiting perivascular characteristics and functions.
    Preview · Article · Jan 2015 · Biochemical and Biophysical Research Communications
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    [Show abstract] [Hide abstract] ABSTRACT: Chlorogenic acid (5-caffeoylquinic acid, CGA) is a phenolic compound that is found ubiquitously in plants, fruits and vegetables and is formed via the esterification of caffeic acid and quinic acid. In addition to its notable biological functions against cardiovascular diseases, type-2 diabetes and inflammatory conditions, CGA was recently hypothesized to be an alternative for the treatment of neurological diseases such as Alzheimer's disease and neuropathic pain disorders. However, its mechanism of action is unclear. Voltage-gated potassium channel (Kv) is a crucial factor in the electro-physiological processes of sensory neurons. Kv has also been identified as a potential therapeutic target for inflammation and neuropathic pain disorders. In this study, we analysed the effects of CGA on the two main subtypes of Kv in trigeminal ganglion neurons, namely, the IK,A and IK,V channels. Trigeminal ganglion (TRG) neurons were acutely disassociated from the rat TRG, and two different doses of CGA (0.2 and 1 mmol⋅L(-1)) were applied to the cells. Whole-cell patch-clamp recordings were performed to observe alterations in the activation and inactivation properties of the IK,A and IK,V channels. The results demonstrated that 0.2 mmol⋅L(-1) CGA decreased the peak current density of IK,A. Both 0.2 mmol⋅L(-1) and 1 mmol⋅L(-1) CGA also caused a significant reduction in the activation and inactivation thresholds of IK,A and IK,V. CGA exhibited a strong effect on the activation and inactivation velocities of IK,A and IK,V. These findings provide novel evidence explaining the biological effects of CGA, especially regarding its neurological effects.International Journal of Oral Science (2014) 6, doi:10.1038/ijos.2014.58; published online 14 November 2014.
    Preview · Article · Nov 2014 · International Journal of Oral Science
  • Hang Wang · Shengnan Sun · Yi Zhang · Si Chen · Ping Liu · Bin Liu
    [Show abstract] [Hide abstract] ABSTRACT: Liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS) and first-dimensional fractionation is widely used for reducing sample complexity in large-scale proteomic profiling experiments. However, the limited number of proteins identified and the relatively long running time are a barrier to the successful application of this approach. In this study, off-line high pH reversed-phase fractionation (RPF) was combined with nano-LC–MS/MS in order to develop an improved method for global proteomic profiling of different cell lines. In the first dimensional reverse phase HPLC separation, 300 μg of digested cell protein was separated into 78 fractions under high pH conditions and condensed into 26 fractions for the second nano-LC–MS/MS analysis at low pH. The chromatographic conditions for the first and second steps were optimized, and the accuracy and reproducibility of protein quantification were investigated with an average Pearson correlation coefficient of 0.94. The method was then applied in the identification of proteins in six common cell lines (DMS, MFM, HepG2, U2OS, 293T and yeast), which resulted in identification of 7300–8500 and 8956 proteins in heavy/light labeled and label-free cell samples, respectively, in 1.5 days. The performance of the developed method was compared with isoelectric focusing (IEF)-nano-LC–MS/MS and the previously reported method; and off-line high pH RPF-nano-LC–MS/MS proved advantageous in terms of the number of proteins identified and the analytical time needed to achieve a successful global proteomic profiling outcome. The RPF-nano-LC–MS/MS method identified more proteins from low abundance (150 μg) samples with an average sequence coverage for each cell line of 23.4–35.1%. RPF-nano-LC–MS/MS may therefore be an efficient alternative tool for achieving improved proteomic coverage of multiple cell lines.
    No preview · Article · Nov 2014 · Journal of Chromatography B
  • [Show abstract] [Hide abstract] ABSTRACT: Masticatory movements have been hypothesized to have a close relationship with the endochondral ossification of mandibular distraction gap tissue. This study investigated whether the local biomechanical environment of the gap tissue contributes to endochondral ossification of new tissue during the early consolidation period of mandibular distraction osteogenesis (DO) in the case of unstable fixation. A 3D finite element model was used to evaluate the compressive hydrostatic pressure stresses and shear stresses of new tissue in the unilateral mandibular DO gap after 4 weeks of consolidation. Four basic clenching conditions—namely clenching in the intercuspal position (ICP), left unilateral molar clenching (L-MOL), right unilateral molar clenching (R-MOL), and incisal clenching (INC)—were investigated. The gap tissue was always subjected to compressive hydrostatic pressure stresses and shear stresses, but the stress value of a specific location varied with clenching conditions. Under ICP, the gap tissue gained the maximum compressive hydrostatic pressure stresses and shear stresses; L-MOL experienced lower compressive hydrostatic pressure stresses and shear stresses, followed by R-MOL, while INC experienced minimum stress. During ICP, L-MOL, and R-MOL conditions, the junction area of lingual and distal surfaces of the gap tissue showed a greater compressive hydrostatic pressure stress, especially the area close to the inferior mandibular border, an area in which endochondral ossification has been observed in vivo during the early phase of consolidation. The results suggest that masticatory movement may contribute to endochondral ossification in the distraction gap in the early phase of consolidation under unstable fixation. During DO, stable fixation is an important factor in ensuring comparability of the results.
  • Hang Wang · Yongkang Luo · Heping Huang · Qian Xu
    [Show abstract] [Hide abstract] ABSTRACT: Investigation on the microbial succession of grass carp filets during storage at 4°C was carried out. For identification, 16S rRNA genes of the isolated pure strains were sequenced and analyzed. Acinetobacter was dominant in fresh grass carp. Species from the genera Brevundimonas, Empedobacter, Pseudomonas, Microbacterium, Flavobacterium, Moraxella, Shewanella and Soonwooa were also detected at the initial day. The communities were dominated by Aeromonas and Acinetobacter after 6days. Aeromonas followed by Pseudomonas was the predominant genera at the end of shelf-life of grass carp, while other genera such as Shewanella, Acinetobacter, Flavobacteriaceae and Psychrobacter were present in smaller numbers. We investigated biogenic amines' (BAs) production by six strains isolated from spoiled grass carp filets. Shewanella putrefaciens showed significantly higher abilities to produce putrescine, than those from other genera. Aeromonas veronii revealed a strong ability to produce putrescine and cadaverine. However, Pseudomonas and Acinetobacter showed little ability to produce BAs.
    No preview · Article · Aug 2014 · International Journal of Food Microbiology
  • Kaifeng Li · Huixing Shen · Bo Li · Hang Wang · Yongkang Luo
    [Show abstract] [Hide abstract] ABSTRACT: In order to understand physicochemical properties of water-soluble proteins obtained from crucian carp, turbidity, total sulfhydryl content, hydrophobicity and SDS-PAGE of crucian carp water-soluble proteins during heat treatment were investigated. Turbidity remained unchanged up to 44°C and considerably increased from 46°C to 54°C, one peak of increase rate was found at 50°C; total SH content decreased rapidly when heated from 50°C to 55°C; hydrophobicity increased sharply when heated up to 45°C, indicating the conformation of water-soluble proteins from crucian carp began to unfold and expose the buried nonpolar amino acids at temperatures above 45°C; analysis of SDS-PAGE indicating the formation of disulfide linkage of creatine kinase and glyceraldehy-3-phosphate dehydrogenase when the temperature reached 65°C and 80°C, respectively.
    No preview · Article · Jul 2014 · Journal of Food Science and Technology -Mysore-

Publication Stats

1k Citations
228.75 Total Impact Points


  • 2009-2015
    • Wuhan General Hospital of Guangzhou Military Command
      Wu-han-shih, Hubei, China
  • 2004-2015
    • Sichuan University
      • • State Key Laboratory of Oral Diseases
      • • Department of Oral and Maxillofacial Surgery
      • • Key Laboratory of Oral Biomedical Engineering
      Hua-yang, Sichuan, China
    • Nanyang Technological University
      • School of Electrical and Electronic Engineering
      Tumasik, Singapore
  • 2013
    • China Agricultural University
      • College of Food Science and Nutritional Engineering
      Peping, Beijing, China
    • China Agriculture University-East
      Peping, Beijing, China
  • 2011-2012
    • Third Military Medical University
      Ch’ung-ch’ing-shih, Chongqing Shi, China
  • 2005-2012
    • West China Hospital of Stomatology
      Hua-yang, Sichuan, China
  • 2005-2008
    • Academisch Centrum Tandheelkunde Amsterdam
      • Field of Dental Material Sciences
      Amsterdamo, North Holland, Netherlands
  • 2007
    • University of Amsterdam
      Amsterdamo, North Holland, Netherlands