Xiaoyu Wang

Huazhong University of Science and Technology, Wu-han-shih, Hubei, China

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Publications (3)9.87 Total impact

  • Xiaomeng Li · Binghui Li · Jun Ma · Xiaoyu Wang · Shengming Zhang
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    ABSTRACT: A sponge wound dressing comprising silk fibroin, N-(2-hydroxy)propyl-3-trimethyl ammonium chitosan chloride, and polyvinyl alcohol was developed for chronic wound healing. These composite sponges were characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. The composite sponge had a fluid uptake of 80% of its weight, and the water vapor transmission rate of 2974 +/- 684 g/m(2)/day, indicating that the sponge could keep a moist environment around the wound bed. The Wistar rats were used to evaluate these composites for the treatment of chronic wounds. Wound healing was monitored through the macroscopic and immunological analyses. Although the wound area reduction rates were similar for the composite dressings compared to the non-woven fabrics containing wax-oil, the new composite dressings were found to be capable of improving the formation of blood vessels inside the wound beds by promoting the regrowth of skin tissues. Based on these results, using aqueous composite sponges in wound dressings, instead of oil-containing fabrics, promotes healing of chronic wounds in clinical applications.
    No preview · Article · Jul 2014 · Journal of Bioactive and Compatible Polymers
  • Bo Xiao · Xiaoyu Wang · Zhiye Qiu · Jun Ma · Lei Zhou · Ying Wan · Shengmin Zhang
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    ABSTRACT: Galactosylated chitosan-hydroxypropyltrimethylammonium (gal-HTCC) was synthesized by galactosylating and quaternizing chitosan to endue chitosan with targeting specificity for potential applications as gene vectors. The composition and physicochemical properties of gal-HTCC were characterized by FT-IR, (1) H NMR, elemental analysis, X-ray diffraction, and turbidity measurement. It was found that water-soluble gal-HTCC showed a more amorphous structure than chitosan, and it also had a much better plasmid condensation capability than galactosylated chitosan. Cytotoxicity measurements revealed that gal-HTCC showed significantly lower cytotoxicity in HepG2 and HeLa cell lines compared to branched polyethylenimine (bPEI, 25 kDa) which was used as a positive control. The nanoparticles (NPs) consisted of gal-HTCC and plasmid DNA had desirable particle size (around 250 nm) with a narrow size distribution. Confocal laser scanning microscopy confirmed that NPs could be internalized and transported to the nucleus efficiently within 6 h. In vitro gene transfection results indicated that gal-HTCC had significantly higher transfection efficiency (7- to 32-fold) compared to chitosan and gal-chitosan for targetable delivery of pGL3 luciferase plasmid to HepG2, and its transfection efficiency was highly inhibited in the presence of galactose (20 mM). All these results suggest that gal-HTCC can function as a promising nonviral gene vector for efficient liver-targeted gene delivery. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
    No preview · Article · Jul 2013 · Journal of Biomedical Materials Research Part A
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    ABSTRACT: A series of N-(2-hydroxy)propyl-3-trimethyl ammonium chitosan chloride (HTCC) samples with various degrees of quaternization ranging from 12.4 to 43.7% was synthesized. The structures and properties of HTCC were investigated by FT-IR, (1)H NMR, conductometric titration and XRD analysis. It was found that HTCC had a more amorphous structure than chitosan. HTCC samples showed significantly lower cytotoxicity than polyethyleneimine in HepG2 and HeLa cell lines. The samples spontaneously formed complexes with pGL3 luciferase plasmid. These complexes had desirable particle sizes (160-300 nm) and zeta potentials (10.8-18.7 mV) when the weight ratios of HTCC to plasmid altered in the range of 3:1-20:1. In vitro gene transfection results indicated that HTCC had significantly high transfection efficiency compared with chitosan for delivering pGL3 luciferase plasmid to HeLa cells. The results suggest that HTCC could be a promising non-viral vector for safe and efficient DNA delivery.
    No preview · Article · Nov 2011 · Colloids and surfaces B: Biointerfaces