[Show abstract][Hide abstract] ABSTRACT: In this study, we investigated the biological effects of RF (Radio Frequency) and microwave in terms of neurological and biochemical findings, and CD4/CD8 ratio in people that work at broadcasting and television transmitter stations. The study was carried out on 26 people from 20 to 49 years of age, occupationally exposed to nonionizing radiation. First we gave out a questionnaire consisting of 12 questions to everybody under investigation. Secondly, we determined the levels of ALT, AST, LDH, ALP, CK, phosphorus, total protein, albumin cholesterol, uric acid, urea, creatinin, Na, K, and NSE (Neuron Specific Enolase), and also CD4/CD8, albumin/globulin ratios and percentage of serum proteins. The results obtained in this study were statistically compared to a control group.
[Show abstract][Hide abstract] ABSTRACT: In this study, we investigated the effects of High Voltage Transmission Lines (HVTL) on people who work and live in such areas. The study was carried out on 51 persons from 15 to 50 years of age exposed to electric field. First, we gave out a questionnaire to everybody under investigation. We established that these people most often suffer from headache, irritability, fatigue, stress and shock. Secondly, we determined 23 parameters of their blood serum. The levels of ALP, Na, Cl, total protein, albumin, Group Specific Component, ceruloplasmin and albumin/globulin ratio were found especially significant.
[Show abstract][Hide abstract] ABSTRACT: In the present experimental study, it was investigated the periodontium of rats that exposed to Extremely Low Frequency-Magnetic Field (ELF-MF), overectomized bilaterally and treated with Strontium Ranelate (SR). The experiments were performed on 75 female Sprague-Dawley (4 months) rats. The rats were divided into five groups (n = 15). All rats were subjected to bilateral ovariectomy except those in I and III groups. Bilateral ovariectomy was performed before 4 days at the beginning of the experiments under ketamine anesthesia (100 mg kg-1, intramuscularly) and II, IV and V groups animals were subjected to 1.5 mT ELF-MF exposure during 6 months, 4 h a day starting 5th day after the surgery. There were statistically significant differences among the all groups in the case of penodontal ligament, alveolar bone andgingiva (p<0.05). As a result of the study there were different levels of histopathologic changes occured between control and experimental changes in the case of all periodontal tissue examination. These results may hypothese that both ELF-MF and Strontium renalate have effects on periodontal tissues.
[Show abstract][Hide abstract] ABSTRACT: By means of long term extremely low frequency magnetic field (ELF), this study examined the effect of long term magnethic field on rat calvarian and mandibular bone mass. Fourty five female Spraque-Dawley rats were selected for the study. The rats were divided into 3 groups (n=15); ELF MF with 50Hz, 1.5mT was applied for 6 months, 4 hours/day. Total cranial and mandibular bone mineral density (BMD) and bone mineral content (BMC) of rats were determined by dual-energy X-ray absorptiometry (DXA; Discovery QDR series, Discovery A, Hologic, Inc.). Measurements were recorded for all groups before and after the applications, all data were collected and analyzed statistically. The variations in Group 1, before and after the ELF MF applications with respect to BMC and BMD were considered significant (p< .01, p< .001). The variations in Group 2, that has bone loss with respect to BMC andBMD were considered not significant (p>0.05). The variations in control group, Group 3, with respect to BMC and BMD were considered significant (p<0.05).
[Show abstract][Hide abstract] ABSTRACT: Exposure to Extremely Low Frequency (ELF) Electromagnetic Field (EMF) emanating from the generation, distribution and utilization of electricity. The major debate in recent years has focused on the possibility that exposure to EMF may result some health consequences such as differentiation on bone constitute. In this study, the effect of ELF-EMFs and strontium ranelate on teeth constitute amount of mineral were investigated in rats. Seventy-five four month old adult female Sprague-Dawley rats were randomly divided into 5 different groups (n = 15). After all applications, some mineral levels such as: Ca, Mg and Zn in rat teeths were determined with Atomic Absorbtion Spectrophotometry (AAS) and the phosphorus content of teeth was determined by Ultraviolet Spectrophotometer (UVS). It was determined that the levels of Ca and P were not statistically different in comparison to Cg-Cnt and between groups (p>0.05). However, it was observed that the levels of Zn significantly alterations between some groups (p<0.05). The levels of Mg in ELF-EMF+OVX, ELF-EMF and OVX groups decreased significantly in comparison to Cg-Cnt group (p<0.05). As a resulf it can be suggested that mineral amount of rat teeth can change after ovariectomy and ELF-EMF exposure, also strontium ranelate treatments can't increase mineral amount of teeth.
Full-text · Article · Jan 2009 · Journal of Animal and Veterinary Advances
[Show abstract][Hide abstract] ABSTRACT: Exposure to extremely low frequency magnetic field (ELFMF) originating from daily electrical-electronic appliances and electric lines, is a common thing. Recently, the discussions focused on the topic whether ELFMF had any harmful effects over health or not. Several previous studies displayed that ELFMF significantly increased bone mineral density. The aim of the present study was to investigate the influence of ELFMF and manganese over mineral accumulation in head and jaw bones of rats. Previous studies showed that manganese plays a significant role over normal development of enamel and dentin alongside contributing to the calcium and phosphorus association. In the present study, the effects of exposure to ELFMF and manganese on mineral density and histology of teeth, were investigated. In the present study, 64 male Wistar albino rats were divided into 8 groups. Amount of mineral elevations were determined in head and jaw bones of the first 6 groups (p<.05) and histologic alterations were found in teeth of rats. The obtained results indicated that ELFMF plays an important role over mineral density and histologic structure of cranial and jaw bones in rats. In conclusion, further epidemiological and experimental laboratory studies are required for more detailed evaluation of the effects of ELFMF on cranial and jaw bones of rats.
[Show abstract][Hide abstract] ABSTRACT: The affects of radiofrequency (RF) and microwave radiation in workplace have been a subject of continuing investigations. One of the major exposed groups is workers and their families who have been working and living in houses provided to employees of these stations. Therefore, the present study intended to investigate the effect of RF emitted from antenna of 1062 kHz medium wave radio-broadcasting station on bioelectrical parameters of workers and their families living in houses provided to employees of these stations. The study was carried out on 20 men working in radio-broadcasting station, 11 women and 30 voluntary children (Boy: 15, Girl: 13) living in houses provided to workers of the station. Five bioelectrical parameters such as resistance, reactance, phase angle, body capacitance and basal metabolic rate (BMR) were measured. E field and power density inside houses was between 0.48 V/m - 2.86 V/m and 0.0001 mW/cm2 - 0.0023 mW/cm2 respectively. However, the average E field and power density in the playing area of the children was 12.75 V/m and 0.0436 mW/cm2. Average E field and power density in the control room desk and around the workplace was 4.04 V/m, 0.0042 mW/cm2 and 31.88 V/m, 0.348 mW/cm2 respectively. The results of this study showed that the parameters such as phase angle (°), body capacitance (pF) and reactance (Ω) of girl group were decreased according to control group (p<0.01). In conclusion, some bioelectrical parameters of the children who have been living in houses provided to employees of radio broadcasting stations can be affected by radiofrequencies.
No preview · Article · Aug 2008 · Biotechnology & Biotechnological Equipment
[Show abstract][Hide abstract] ABSTRACT: In the present study, the effect of low-intensity microwave radiation on rat eyes and the protective effect of some vitamins against the damage induced by microwave was investigated. 32Wistar-albino female rats were divided into four groups (1 Control, 3 Experimental) (n=8). The rats of first experimental group were exposed to 9450 MHz microwave (2.65 mW/cm2) for one hour a day during 21 days. In addition to exposure with MW as first experimental group, Vitamin E and Vitamin C (150 mg/kg/day) were injected intraperitoneally to second and third experimental groups respectively during last seven days of exposure. The eyes of rats were examined daily after MW and sham exposure by an ophthalmologist biomicroscopically. After completed 21 days exposure period, the rats were sacrificed under ketalar anesthesia. The rat eyes were enucleated for histopathological examination and Malondialdehyde (MDA) assay. Sero hemoragic dark brown secretion was observed in rats' conjunctiva of fist and second experimental groups after biomicroscopic examination. However, no damage was detected in the third group. Vacuolization, pleomorphism between cells and increasing of cell layers in lens fibers of all experimental groups were observed histopathologically. In the cornea of experimental groups, cellular loss, shape and size differences among cells and lack of polarity were noticed. Slight edema, congestion, desquamation in epithelial cells, separation between layers in some areas and alteration in pigment cells of retina were also determined in experimental group rats. The MDA (Malondialdehyde) levels of all experimental groups increased significantly compared to control group (P<0.05). However, no significant differences among experimental groups were observed at MDA levels (P>0.05). The temperature differences of all experimental groups were found to be significant compared to control group (P<0.05). Finally, it was determined that low-intensity long-term microwave exposure caused histopathologic alternations and lipid peroxidations on rat eyes.
[Show abstract][Hide abstract] ABSTRACT: This investigation was carried out in order to determine the protective effects of long term administration at supraphysiological doses of vitamin E, vitamin C and a compound of vitamin E + glutathione against damage induced by free radicals produced by ionizing radiation. Five groups of 8-10-week-old Wistar albino rats were used in the study. Group I was the sham group and Group 11 was the radiation group (i.e. radiation alone). The rats in Group III were given a daily dose of 400 mg/kg of vit. C for one month. The rats in Group IV were given a daily dose of vit. E for one month. The rats in Group V received a mixture of 400 mg/kg vit. E plus 1000 mg/kg of glutathione per day for 15 days. All the compounds were administered via a nasogastric tube. At the end of the drug administration period, the rats in all but the sham group were exposed to X-ray with an radiation energy dose of 8.90 Gy. All the rats were sacrificed 1 day after irradiation. Thyroid and intestinal tissues were examined for histopathological changes and T3, T4, TSH hormone levels were determined by radioimmunoassay (RIA). The radiation damage observed in the thyroidal and intestinal cross-sections of the Group II rats was also seen to a lesser extent in the Group III (vit. C group) and Group IV (vit. E) rats. Toxic effects were observed in the Group V (vit. E + glutathione) rats. Significant T3 and TSH level changes (p<0.001; p<0.01) were observed in the Group II rats (radiation alone) and the levels of these hormones appeared to be partly affected in the rats in Group III (vit. C group) and Group V (vit. E + glutathione), albeit insignificantly (p>0.05).
No preview · Article · Jan 2000 · Turkish Journal of Medical Sciences
[Show abstract][Hide abstract] ABSTRACT: This study investigated whether there are adverse effects due to microwave exposure emitted by cellular phones in male rats. Eighteen Wistar Albino rats were separated into three groups, a sham group and two experimental groups. The rats were confined in Plexiglas cages and cellular phones were placed 0.5 cm under the cages. In the first experimental group, cellular phones were in standby position for 2 h. In the second experimental group, phones were turned to the speech position three times each for 1 min duration over 2 h. Rats in the first and second experimental groups were exposed to microwaves emitted by phones for 2 h/day for a duration of 1 month. After the last exposure the rats were killed. Brain, eyes, ears, liver, heart, lungs, stomach, kidneys, testes, small and large intestines and skin of the rats were observed histologically. The decrease of epididymal sperm counts in the speech groups were not found to be significant (P > 0.05). Differences in terms of normal and abnormal sperm forms were not observed (P > 0.05). Histological changes were especially observed in the testes of rats of the speech groups. Seminiferous tubular diameter of rat testes in the standby and speech groups was found to be lower than the sham group (P < 0.05). Rectal temperatures of rats in the speech group were found to be higher than the sham and standby groups (P < 0.05). The rectal temperatures of rats before and after exposure were also found to be significantly higher in the speech group (P < 0.05). Specific absorption rate (SAR) was determined as 0.141 W/kg.
Full-text · Article · Jul 1999 · Urological Research
[Show abstract][Hide abstract] ABSTRACT: The aim of this study was to investigate the effect of chronic microwave exposure on sperm count, sperm morphology, and the morphology of rat testis and epididymes. Continuous wave microwave radiation (9450 MHz) specific absorption rate 1.80 W/kg and power density 2.65 mW/cm(2), was administered for 1 h a day for 13, 26, 39, and 52 days, which corresponded to 1, 2, 3, and 4 cycles of seminiferous epithelium, in mature male Sprague-Dawley rats. The rats were sacrificed under Ketalar anesthesia the day after exposure. Then epididymal sperm count, sperm morphology, and the weights of testis, epididymes, seminal vesicles, and prostate were determined. Histologic examinations of testis and epididymes were performed. The parameters were compared with sham groups. Epididymal sperm count decreased significantly only in the 52-day exposure group (p < 0.05). The percentage of abnormal sperm count changed significantly in the 26-, 39-, and 52-day exposure groups (p < 0.05, p < 0.05, and p < 0.001, respectively). The weights of testis and epididymes also changed significantly in the 26-, 39-, and 52-day exposure groups (p < 0.05, p < 0.05, and p < 0.05, respectively). Necrotic tubules, interstitial edema, perforated and necrotic tubules, decrease of spermatogenesis, and absent germinal epithelium in some tubules were observed in the exposed rat testes. In addition, we observed atrophy, interstitial edema, mononuclear cell infiltration, and increased fibroblastic activity in the exposed rat epididymes. We concluded that epididymal sperm count and morphology and weight and morphology of testis and epididymes were affected by chronic prolonged microwave exposure. The incidence of symptoms mentioned here depended on exposure duration.
No preview · Article · Jan 1999 · Electromagnetic Biology and Medicine
[Show abstract][Hide abstract] ABSTRACT: The present article intended to investigate some hormone levels of technicians in the radio-broadcasting, television (TV) and radio - link stations. The study was carried out on 43 technicians from 20 to 59 years age occupationally exposed to radiofrequency (RF) and microwave (MW) radiation. 20 unexposed and voluntary persons from 20 to 59 years age were accepted as a control group. We determined the levels of some hormones as triiodothyronine (T3), tetraiodothyronine (T4), thyroid stimulant hormone (TSH), free T4, estradiol, dehydroepiondrosterone sulfate (DHEA), testosterone, cortisol and progesterone in the technicians under investigation. The hormone levels of exposure groups were statistically compared with the unexposed control group. T3, T4, estradiol, testosterone and progesterone levels of technicians in the radio-link and TV transmitter stations were found to be significant (p < 0.05, p < 0.001). T3, T4, TSH, estradiol and progesterone levels of technicians in the broadcasting station were also found to be significant (p < 0.001). In conclusion some hormone levels of technicians under investigation were altered by RF/MW.