Hélène Corbière

Universität Basel, Basel, BS, Switzerland

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Publications (3)5.81 Total impact

  • Peter Salzer · Hélène Corbière · Thomas Boller
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    ABSTRACT: The diaminobenzidine (DAB) staining technique was used to examine the accumulation of H2O2 in parts of roots of Medicago truncatula Gaertn. colonized by the arbuscular mycorrhiza (AM)-forming fungus Glomus intraradices Schenk and Smith. At the cellular level, the combination of bright-field and fluorescence microscopy revealed that a brownish stain, indicative of H2O2 accumulation was present within cortical root cells in the space occupied by arbuscules. Accumulation of H2O2 was especially pronounced in cells containing arbuscules that were clumped and less branched. Moreover, H2O2 accumulated around hyphal tips attempting to penetrate a host cell. In contrast, no H2O2 accumulation was observed in hyphal tips growing along the middle lamella, or in appressoria or vesicles. On the basis of these findings we suggest that a locally restricted oxidative burst is involved in the temporal and spatial control of the intracellular colonization of M. truncatula cells by the AM-forming fungus G. intraradices.
    No preview · Article · Apr 1999 · Planta
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    ABSTRACT: Anumber of research groups have recently succeeded in down regulating cinnamyl alcohol dehydrogenase (CAD EC. activity through genetic engineering with the aim of modifying lignin profiles in plants. In view of the expected extension of this technology to economically important plants, we have tried to obtain additional information on the best antisense constructs for maximal CAD down regulation using tobacco (Nicotiana tabacum cv. Samsun) as a model system. A comparison of different constructs showed that efficiency was greater with homologous cDNAs (partial or full-length sequence) vs heterologous cDNA. In addition, constitutive promoters were, in our hands, more efficient than tissue (xylem) specific promoters. Although a strong down regulation of CAD gene expression was obtained for different transformants at the in vitro stage, a drop in CAD activity reduction was observed for some of them during development in the greenhouse. These different aspects are discussed in the context of future applications.
    No preview · Article · Sep 1998 · Phytochemistry
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    Hélène Louise Françoise. Corbière
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    ABSTRACT: Diss. Naturwiss. Basel (kein Austausch). Literaturverz. Universität
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