Jiajia Xia's scientific contributionswhile working at Nanyang Technological University (Singapore, Singapore) and other institutions

Publications (15)

  • Abstract: Analysis of the RNP nuclear export in AIV-infected A549 cells. A549 cells were infected with either the H1N1/WSN, H9N2, H5N2/F118 or H5N3 viruses using an MOI = 4. At specific times post infection the cells were fixed and labelled using anti-NP and goat anti-mouse conjugated to Alexa555. The stained cells were visualised using a Nikon Eclipse 80i Microscope at ×20 magnification with appropriate machine settings. The NP-stained nuclei (*) and cells (white arrow) are indicated. (TIF)
    File available · Data · Mar 2012
  • Abstract: Analysis of the RNP nuclear export in pH1N1 virus-infected MDCK or CEF cells. Cells were infected with either the H1N1/WSN, pH1N1/471 or pH1N1/527 viruses using an MOI = 4. At specific times post infection the cells were fixed and labelled using anti-NP and goat anti-mouse conjugated to Alexa555. The stained cells were visualised using a Nikon Eclipse 80i Microscope at ×20 mag with appropriate machine settings. The stained cells are indicated (white arrow). (TIF)
    File available · Data · Mar 2012
  • Abstract: Relative expression of the selected interferon (IFN) and IFN-stimulated genes (ISGs) as measured by qPCR. A549, MDCK and CEF cells were infected with either H1N1/WSN, H9N2, H5N2/F118 or H5N3 viruses at an MOI = 4, and analysed at 10 hpi. The average values and standard error were obtained from three independent experiments (p<0.05). IFN-β1: interferon β; MX1: myxovirus resistance 1; OAS1: 2′, 5′-oligoadenylate synthase 1; OAS2: 2′, 5′-oligoadenylate synthase 2; OASL: 2′, 5′-oligoadenylate... Show More
    File available · Data · Mar 2012
  • Abstract: Cell-to-cell transmission in H1N1/WSN and H9N2 virus-infected A549 cell monolayers. Near confluent A549 cell monolayers were infected with either the H1N1/WSN or H9N2 viruses using an MOI = 0.01 in DMEM containing 1 µg/ml TPCK trypsin and 0.21% BSA at 37°C. At 24 hpi the cells were fixed and labelled using anti-NP and anti-mouse IgG conjugated to FITC. The labelled cells were viewed using a fluorescence microscope and the nuclei are highlighted (white arrow). Insets show the staining pattern... Show More
    File available · Data · Mar 2012
  • Abstract: Analysis of the RNP nuclear export in AIV-infected MDCK and CEF cells. The cells were infected with either the H1N1/WSN, H9N2, H5N2/F118 or H5N3 viruses using an MOI = 4, and at specific times post infection the cells were fixed and labelled using anti-NP and goat anti-mouse conjugated to Alexa555. The stained cells were visualised using a Nikon Eclipse 80i Microscope at ×20 magnification with appropriate machine settings. The NP-stained nuclei (*) and cells (white arrow) are indicated. (TIF)
    File available · Data · Mar 2012
  • Abstract: Overview of selected gene families, showing significantly up-regulated expression in A549 cells infected with influenza viruses. A549 cells were infected with either the H1N1/WSN, H9N2, H5N2/F118, H5N2/189, H5N3 or pH1N1/527 viruses at an MOI = 4 and analysed at 10 hpi. The proportion of probe sets in the different gene families, including non-annotated and unclassified gene groups, showing greater than 2-fold change in gene expression and those showing greater than 10-fold change in gene... Show More
    File available · Data · Mar 2012
  • Abstract: Analysis of the RNP nuclear export in pH1N1 virus-infected A549 cells. Cells were infected with either the H1N1/WSN, pH1N1/471 or pH1N1/527 viruses using an MOI = 4. At specific times post infection the cells were fixed and labelled using anti-NP and goat anti-mouse conjugated to Alexa555. The stained cells (highlighted by white arrow) were visualised using a Nikon Eclipse 80i Microscope at ×20 magnification with appropriate machine settings. (TIF)
    File available · Data · Mar 2012
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    Abstract: The host response to the low pathogenic avian influenza (LPAI) H5N2, H5N3 and H9N2 viruses were examined in A549, MDCK, and CEF cells using a systems-based approach. The H5N2 and H5N3 viruses replicated efficiently in A549 and MDCK cells, while the H9N2 virus replicated least efficiently in these cell types. However, all LPAI viruses exhibited similar and higher replication efficiencies in CEF cells. A comparison of the host responses of these viruses and the H1N1/WSN virus and low passage... Show More
    Full-text available · Article · Mar 2012 · PLoS ONE
  • Abstract: Comparison of gene expression values of IFN and selected ISGs in influenza virus-infected CEF cells. A FC value of 1 indicates not significantly changed. These are the results of 3 independent experiments, (p<0.05). (TIF)
    File available · Data · Mar 2012
  • Abstract: Plaque formation in MDCK cells infected with influenza virus. Near confluent MDCK cells were infected either with the H1N1/WSN, H5N2/F118, H5N3 or H9N2, and virus plaque formation monitored using an agarose overlay plaque assay over a 7 day period. The start of plaque formation (indicated by the black arrow) and the centre of the final plaque (*) imaged at 7 days post-infection are highlighted. (TIF)
    File available · Data · Mar 2012
  • Abstract: Comparison of gene expression values of selected ISGs in influenza virus-infected MDCK cells. A FC value of 1 indicates not significantly changed. These are the results of 3 independent experiments, (p<0.05). (TIF)
    File available · Data · Mar 2012
  • Abstract: Interferon, selected ISG and host genes involved in cell death pathways in influenza virus-infected A549 cells. A FC value of 1 indicates not significantly changed. These are the results of 3 independent experiments, (p<0.05). (TIF)
    File available · Data · Mar 2012
  • Abstract: Overview of selected gene families, showing significantly down-regulated expression in A549 cells infected with influenza viruses. A549 cells were infected with either the H1N1/WSN, H9N2, H5N2/F118, H5N2/189, H5N3 or pH1N1/527 viruses at MOI = 4 and at 10 hpi, were analysed. The proportion of probe sets in the different gene families, including non-annotated and unclassified gene groups, showing less than −2-fold change in gene expression and those showing less than −10-fold change in gene... Show More
    File available · Data · Mar 2012
  • Abstract: Temporal changes in the host cell transcriptome during influenza virus infection. A549, CEF and MDCK cell monolayers were either mock-infected or infected with H1N1/WSN, pH1N1/527, H5N2/F118 and H9N2 using an MOI = 4. The global host gene expression profiles in virus-infected cells were compared to mock-infected cells by microarray analysis. The fold change in gene expression in virus-infected cells is shown at each time point examined. The data were obtained from 3 independent experiments,... Show More
    File available · Data · Mar 2012
  • Abstract: Primer and probes sequences designed for real-time qPCR. Primer sequences and UPL probes (Roche) used for real-time qPCR validation of influenza M gene segment and selected canine, chicken and human host genes. IFN-β1: interferon β; MX1: myxovirus resistance 1; OAS1: 2′, 5′-oligoadenylate synthase 1; OAS2: 2′, 5′-oligoadenylate synthase 2; OASL: 2′, 5′-oligoadenylate synthase-like; RSAD2: radical S-adenosyl methionine domain containing 2; IL28: interleukin 28 (interferon λ2); BIRC4BP: XIAP... Show More
    File available · Data · Mar 2012

Publications citing this author (33)

    • Differential expression of proteins induced by human cells infected by AIV at different time points mainly involved cytoskeleton, cytokine mediated signaling pathways, mRNA transcription and TABLE 4 | Summary of downregulated proteins in A549 cells infected with influenza A H9N2 virus at 72 hpi (r > 2, p < 0.05). Liu et al., 2008; Sutejo et al., 2012). In the current study, an overview of the differentially expressed proteins in response to infection by the H9N2 AIV was obtained by comparing differences in the abundance of proteins isolated from AIV-infected and mock-infected A549 cells.
    [Show abstract] [Hide abstract] ABSTRACT: In this study, differentially expressed proteins in A549 cells (human lung adenocarcinoma epithelial cell line) infected with H9N2 avian influenza virus (AIV) were investigated by two-dimensional electrophoresis (2-DE). Sixteen different spots between the groups (ratio > 2, p < 0.05) were identified with mass spectrometry identification. Proteins located in the downstream of the NF-κB and IFN transcription factor pathways were identified, e.g., ISG15. Actin and keratin were also identified, suggesting that the cytoskeleton may plays an important role in the AIV infection of mammalian cells. These findings could provide insights into the interaction between host and influenza viruses and might provide valuable information for clarifying the pathogenesis of viral infections as well.
    Full-text · Article · Dec 2016