P Jalc

Slovak Academy of Sciences, Presburg, Bratislavský, Slovakia

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Publications (25)35.92 Total impact

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    ABSTRACT: 1. The effect of hemisection of the cervical spinal cord on NADPH-diaphorase staining in the reticular nuclei of the rabbit medulla was investigated using histochemical technique. 2. A quantitative assessment of somal and neuropil NADPH-diaphorase staining was made by an image analyzer in a selected area of each reticular nucleus of the rabbit medulla. 3. On the 7th postsurgery day, the highest up-regulation of somatic NADPH-diapho- rase staining was observed in regions regulating cardiorespiratory processes; however, the highest increase of neuropil NADPH-diaphorase staining was found in the reticular nuclei modulating the tonus of postural muscles. 4. The degeneration of non-NADPH-diaphorase-stained neurons was detected throughout the reticular formation of the medulla, but the extent of neuronal death did not correlate with the up-regulation of the NADPH-diaphorase staining in the reticular nuclei of the medulla. 5. The findings provide evidence that NADPH-diaphorase-exhibiting neurons are refractory to the hemisection of the cervical spinal cord and that the neuronal up-regulation of NADPH-diaphorase at the medullar level is probably not a causative factor leading to the death of the reticulospinal neurons.
    No preview · Article · Jan 2005 · Cellular and Molecular Neurobiology
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    ABSTRACT: In order to clarify whether the midthoracic spinal cord constriction affects nitric oxide synthase (NOS) activity equally with the cyclic 3′,5′-guanosine monophosphate (cGMP) formation, both compounds known to form NO-cGMP signaling pathway were identified in the dorsal, lateral and ventral white matter columns and in non-compartmentalized white matter. The extent of white matter damage was determined in segments Th5-Th6 located rostrally and in segments Th8-Th9 located caudally to the epicenter of the spinal cord injury. The results have shown that traumatic injury, produced at Th7 segment level affected both compounds of NO-cGMP signaling pathway in similar manner. While significant decrease of NOS activity and cGMP level was detected in the ventral columns in segments located rostrally to the constriction site, no such reduction of both compounds forming NO-cGMP signaling pathway was seen in Th8-Th9 segments. Similar, diametrally opposed effect of Th7 segment constriction was found in lateral columns, where a significant increase of NOS activity and cGMP level was detected in Th8-Th9 segments. No significant decrease in NOS activity and cGMP level in response to spinal cord constriction was found in Th5-Th6 segments.
    No preview · Article · Dec 2002 · Biologia - Section Cellular and Molecular Biology
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    ABSTRACT: Changes in the level of cyclic 3',5'-guanosine monophosphate (cGMP) were studied one day after a surgically induced spinal cord constriction performed at the Th7 segment level in the dorsal, lateral and ventral white matter columns and in the non-compartmentalized white matter of Th5-Th6 segments, i.e., above the site of the spinal cord constriction and in Th8-Th9 segments, located below the spinal cord constriction. The midthoracic spinal cord constriction caused a significant decrease in the level of cGMP in the ventral column of Th5-Th6 segments and a significant increase in the lateral column of Th8-Th9 segments. The level of cGMP in the dorsal column, located either rostrally or caudally to the site of the spinal cord injury, remained unchanged. In addition, no significant changes in the level of cGMP were found in the non-compartmentalized white matter of Th5-Th6 and Th8-Th9 segments in response to constriction of the Th7 segment.
    No preview · Article · Nov 2001 · Neurochemistry International
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    ABSTRACT: Changes in the level of cyclic 3′,5′-guanosine monophosphate (cGMP) were studied one day after a surgically induced spinal cord constriction performed at the Th7 segment level in the dorsal, lateral and ventral white matter columns and in the non-compartmentalized white matter of Th5–Th6 segments, i.e., above the site of the spinal cord constriction and in Th8–Th9 segments, located below the spinal cord constriction. The midthoracic spinal cord constriction caused a significant decrease in the level of cGMP in the ventral column of Th5–Th6 segments and a significant increase in the lateral column of Th8–Th9 segments. The level of cGMP in the dorsal column, located either rostrally or caudally to the site of the spinal cord injury, remained unchanged. In addition, no significant changes in the level of cGMP were found in the non-compartmentalized white matter of Th5–Th6 and Th8–Th9 segments in response to constriction of the Th7 segment.
    No preview · Article · Oct 2001 · Neurochemistry International
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    ABSTRACT: An experimental model of the neuropathic pain consisting in the transaction of the sciatic nerve was used to study the dynamics of the neuronal and nonneuronal changes in L5 and L6 dorsal root ganglia with regard to the reduced nicotinamide adenine dinucleotide phosphate diaphorase staining on 7th and 14th postsurgery days. At both surviving periods a statistically significantly increase of the medium-sized dorsal root ganglia neurons was found including the staining of their intraganglionic fibers. A small number of extremely small neurons was detected as well by this histochemical approach considered to be a reliable marker for nitric oxide synthase activity. Qualitative analysis of reduced nicotinamide adenine dinucleotide phosphate diaphorase of the medium-sized neurons supports the view that this neuronal type is activated during the state of the neuropathic pain. It may be concluded therefore that this neuronal type might help in the development of the tactile allodynia and thermal hyperalgesia known to occur during neuropathic pain.
    No preview · Article · Jan 2001 · Biologia
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    ABSTRACT: The level of cGMP was studied one day after constriction of Th7 segment in the dorsal, lateral and ventral white matter columns in Th5-Th6 segments located rostrally and in Th8-Th9 segments, located caudally to the site of the spinal cord constriction. The midthoracic spinal cord constriction caused a significant decrease in the level of cGMP in the ventral column of Th5-Th6 segments. The level of cGMP significantly increased in the lateral column, located caudally to the site of the spinal cord injury. Our data revealed the trauma-evoked changes of cGMP level in the white matter columns of the spinal cord close to the site of constriction.
    No preview · Article · Jan 2001
  • N. Lukan · A. Fercakova · P. Jalc
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    ABSTRACT: Present study shows lipofuscin accumula! ion and morphological characteristics of the pigment granules in the rat spiral ganglion after 30 min global brain ischemia with subsequent 3 day survival. Electron microscopic examination of the spiral ganglion showed sporadic presence of lipofuscin granules in neurons and its occasional occurrence in the satellite cell in normal adult animals. Progressive accumulation of lipofuscin in type I neurons S days postischcmia was noticed. Four different types of lipofuscin granules have been described, ranging from small, round and homogeneously dark bodies containing clear lipid-like vacuoles, to the complicated structures with number of vacuoles and ''fingerprint patterns. Pigment bodies were also seen in the surrounding satellite cells. Morphological analysis of lipofuscin accumulation in the poslischcmic period revealed a higher incidence of ceroid-like grannies, which seems to be related to the peroxidative membrane damage due to ischemia.
    No preview · Article · Dec 2000 · Biologia
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    ABSTRACT: The distribution and changes of catalytic nitric oxid synthase (cNOS) activity in the dorsal, lateral and ventral white matter columns at midthoracic level of the rabbit's spinal cord were studied in a model of surgically-induced spinal cord constriction performed at Th7 segment level and compared with the occurrence of nicotinamide adenine dinucleotide phosphate diaphorase expressing and neuronal nitric oxide synthase immunoreactive axons in the white matter of the control thoracic segments. Segmental and white-column dependent differences of cNOS activity were found in the dorsal (141.5 +/- 4.2 dpm/microm protein), lateral (87.3 +/- 11.5 dpm/microm protein) and ventral (117.1 +/- 7.6 dpm/microm protein) white matter columns in the Th5-Th6 segments and in the dorsal (103.3 +/- 15.5 dpm/microm protein), lateral (54.9 +/- 4.9 dpm/microm protein), and ventral (86.1 +/- 6.8 dpm/microm protein) white matter columns in the Th8-Th9 segments. A surgically-induced constriction of Th7 segment caused a disproportionate response of cNOS activity in the rostrally (Th5-Th6) and caudally (Th8-Th9) located segments in both lateral and ventral white matter columns. While a statistically significant decrease of cNOS activity was detected above the constriction site in the ventral columns, a considerable, statistically significant increase of cNOS activity was noted in the white lateral columns below the site of constriction. It is reasoned that the changes of cNOS activity may have adverse effects on nitric oxide (NO) production in the white matter close to the site of constriction injury, thus broadening the scope of the secondary mechanisms that play a role in neuronal trauma.
    No preview · Article · Sep 2000 · Neurochemical Research
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    J Marsala · P Jalc
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    ABSTRACT: The aim of this study was the histochemical characterization of NADPH diaphorase-positive neuronal pools in the rabbit lumbosacral segments using a model of single, repeated and multiple sublethal spinal cord ischemia. Following a single 8-min sublethal spinal cord ischemia and 1-hour reperfusion, the staining of NADPH diaphorase-exhibiting neurons in the dorsal horn, pericentral region, dorsal gray commissure and sacral parasympathetic nucleus was comparable with the control sections. In contrast to the foregoing sublethal ischemia, a regionally different somatic NADPH diaphorase (NADPHd) staining was found after multiple sublethal spinal cord ischemia. Whereas an almost complete loss of the staining of large NADPHd-exhibiting somata in the pericentral region was detected, the staining of the NADPHd-exhibiting neuronal pools in the deep dorsal horn and sacral parasympathetic nucleus was fully preserved. Concomitantly, a prominent reduction of small NADPH diaphorase-positive neurons was noted in the superficial dorsal horn layers of lower lumbar and sacral segments.
    Preview · Article · Feb 2000 · Physiological research / Academia Scientiarum Bohemoslovaca
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    ABSTRACT: Segmental and laminar distribution of Fos-like immunoreactive, reduced nicotinamide adenine dinucleotide phosphate diaphorase (NADPHd)-exhibiting and double-labeled (Fos-like immunoreactive and NADPHd-exhibiting) neurons was examined in lower lumbar and sacral segments of the dog spinal cord using the model of multiple cauda equina constrictions. NADPHd histochemistry was used as marker of nitric oxide synthase-containing neurons. The appearance and the time-course of Fos-like immunoreactive, NADPHd and double-labeled neurons was studied at 2 h and 8 h postconstriction characterized as the incipient phase of cauda equina syndrome. The occurrence of Fos-like immunoreactive and NADPHd-exhibiting neurons in fully developed cauda equina syndrome was studied at five days postconstriction. An increase in Fos-like immunoreactivity in superficial laminae (I-II) and an enhanced NADPHd staining of lamina VIII neurons were found. A statistically significant increase in Fos-like immunoreactive neurons was found in laminae I-II and VIII-X 8 h postconstriction, and in contrast, a prominent decrease in Fos-like immunoreactive neurons was found in laminae I-II, accompanied by a statistically significant increase in Fos-like immunoreactive neurons in more ventrally located laminae VII-X at five days postconstriction. Quantitative analysis of laminar distribution of constriction-induced NADPHd-exhibiting neurons revealed a considerable increase in these neurons in laminae VIII-IX 8 h postconstriction and a statistically highly significant increase in NADPHd-exhibiting neurons in laminae VII-X five days postconstriction. Concurrently, the number of NADPHd-exhibiting neurons in laminae I-II was greatly reduced. While a low number of double-labeled neurons was found throughout the gray matter of lower lumbar and sacral segments at 2 h postconstriction, a statistically significant number of double-labeled neurons was found in lamina X 8 h and in laminae VII-X five days postconstriction. The course and distribution of anterograde degeneration resulting five days after multiple cauda equina constrictions are compared with segmental and laminar distribution of Fos-like immunoreactive and NADPHd-exhibiting neurons. Prominent involvement of the spinal cord neurons appearing in the lumbosacral segments at the early beginning and in fully developed cauda equina syndrome results in a Fos-like immunoreactivity and strongly enhanced NADPHd staining of some neuronal pools. Under such circumstances, an early cauda equina decompression surgery is advisable aimed at decreasing or preventing the derangement of the neural circuits in the lumbosacral segments.
    Full-text · Article · Feb 2000 · Neuroscience
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    ABSTRACT: The distribution and changes of catalytic nitric oxid synthase (cNOS) activity in the dorsal, lateral and ventral white matter columns at midthoracic level of the rabbit''s spinal cord were studied in a model of surgically-induced spinal cord constriction performed at Th7 segment level and compared with the occurrence of nicotinamide adenine dinucleotide phosphate diaphorase expressing and neuronal nitric oxide synthase immunoreactive axons in the white matter of the control thoracic segments. Segmental and white-column dependent differences of cNOS activity were found in the dorsal (141.5 4.2 dpm/m protein), lateral (87.3 11.5 dpm/m protein) and ventral (117.1 7.6 dpm/m protein) white matter columns in the Th5-Th6 segments and in the dorsal (103.3 15.5 dpm/m protein), lateral (54.9 4.9 dpm/m protein), and ventral (86.1 6.8 dpm/m protein) white matter columns in the Th8-Th9 segments. A surgically-induced constriction of Th7 segment caused a disproportionate response of cNOS activity in the rostrally (Th5-Th6) and caudally (Th8-Th9) located segments in both lateral and ventral white matter columns. While a statistically significant decrease of cNOS activity was detected above the constriction site in the ventral columns, a considerable, statistically significant increase of cNOS activity was noted in the white lateral columns below the site of constriction. It is reasoned that the changes of cNOS activity may have adverse effects on nitric oxide (NO) production in the white matter close to the site of constriction injury, thus broadening the scope of the secondary mechanisms that play a role in neuronal trauma.
    No preview · Article · Jan 2000 · Neurochemical Research
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    ABSTRACT: The distributions of neuronal nitric oxide synthase-immunoreactive neurons and of nicotinamide adenine dinucleotide phosphate-diaphorase activity were studied in the C6, Th2, L1, L5, S2 and S3 segments and laminae in the rabbit spinal cord and compared with the catalytic nitric oxide synthase activity, determined by monitoring the conversion of [3H]arginine to [3H]citrulline in the same segments and laminae. Morphologically, a heterogeneous population of nicotinamide adenine dinucleotide phosphate-diaphorase-expressing and neuronal nitric oxide synthase-immunoreactive neurons was detected in the superficial and deep dorsal horn and the pericentral region in all segments studied, and in the intermediolateral cell column of the thoracic and lumbosacral segments. A disproportionate distribution of both neuronal categories which had a significantly higher number of nicotinamide adenine dinucleotide phosphate-diaphorase-expressing rather than neuronal nitric oxide synthase-immunoreactive cell bodies was found in all segments. The catalytic nitric oxide synthase activity was distributed unequally in the C6, Th2, L1, L5, S2 and S3 segments, with a comparatively low value in the Th2 segment (70±5.1 d.p.m./μg protein) in comparison with the S3 segment, where the highest level (140±5.5 d.p.m./μg protein) was found. A close correlation between the number of neuronal nitric oxide synthase-immunoreactive somata and catalytic nitric oxide synthase activity was revealed in the dorsal horn (laminae I–VI). Whereas a low number of neuronal nitric oxide synthase-immunoreactive somata in laminae VII–X was found in the L5, S2 and S3 segments, the values of catalytic nitric oxide synthase activity in the same laminae and segments were found to be exceedingly high.
    No preview · Article · Sep 1999 · Neuroscience
  • A. Ferčáková · P. Jalč
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    ABSTRACT: We studied the influence of pre-and post-ischaemic treatment with a free radical scavenger, N-acetylcysteine (NAC), on trigeminal ganglia neurons in the rat after 30 min global brain ischaemia. Three experimental groups of animals a) ischaemic non-treated, b) preischaemically and c) postischaemically NAC-treated (400 mg/kg i. p.) rats were used in this study. Changes in the ganglion neurons after ischaemia with 1-3 days survival were studied by light and electron microscopy. Light microscopy in non-treated animals at 3 days post ischaemia revealed some large A-type chromatolytic neurons with dark eccentric nuclei. Small B-type neurons showed heavy vacuolation. Electron microscopy after 3 days survival revealed changes in both types of neurons, characterized by a peripheral location of endoplasmic reticulum, eccentric nuclei and segregated nucleoli. In the cytoplasm of A-type neurons, formation of peripheral vacuoles, lamellar myelinoid bodies and a higher amount of lipofuscin granules were noticed. Numerous B-cells displayed degenerative changes with an extensive mitochondrial vacuolation. Pre- and post-ischaemic NAC administration reduced the occurrence of degenerating B-cells (7.4% or 8.9 %, respectively) compared with ischaemic non-treated rats (14.5%). This protective effect of NAC application may have a value in the treatment of some cerebrovascular diseases.
    No preview · Article · Jan 1999 · Biologia - Section Cellular and Molecular Biology
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    ABSTRACT: The distribution of somatic, fibre-like and punctate, non-somatic reduced nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase activity was examined in dog spinal cord using horizontal, sagittal and transverse sections. The morphological features of NADPH diaphorase exhibiting neurons divided into six different neuronal types (N1-N6) were described and their laminar distribution specified. Major cell groups were identified in the superficial dorsal horn and around the central canal at all spinal levels, and in the intermediolateral cell column at thoracic level. NADPH diaphorase exhibiting neurons of the pericentral region were distributed in a thin subependymal cell column containing longitudinally-arranged small bipolar neurons with processes penetrating deeply into the intermediolateral cell column and/or running rostrocaudally in the subependymal layer. The second pericentral cell column located more laterally in lamina X contains large, intensely-stained NADPH diaphorase exhibiting neurons with long dendrites radiating in the transverse plane. Neurons of the sacral parasympathetic nucleus seen in segments S1-S3 exhibited prominent NADPH diaphorase activity accompanied by heavily-stained fibres extending from Lissauer's tract through lamina I along the lateral edge of the dorsal horn to lamina V. A massive dorsal gray commissure, with high NADPH diaphorase activity, was found in segments S1-S3. At the same segmental level a prominent group of moderately-stained motoneurons was detected in the dorsolateral portion of the anterior horn. Fibre-like NADPH diaphorase activity was found in the superficial dorsal horn and pericentral region in all segments studied. Punctate, non-somatic NADPH diaphorase activity was detected in the superficial dorsal horn, in the pericentral region all along the rostrocaudal axis and in the nucleus phrenicus (segments C4-C5), nucleus dorsalis (segments Th2-L2), nucleus Y (segments S1-S3), and the dorsal part of the dorsal gray commissure (S1-S3). A schematic diagram documenting the segmental and laminar distribution of NADPH diaphorase activity is given.
    No preview · Article · Sep 1998 · Neuroscience
  • Nadežda Lukáčová · Pavol Jalč · J Marsala
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    ABSTRACT: Ischemia-reperfusion induced changes in concentration of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI) and sphingomyelin (SM) in the gray matter taken in toto, white matter, dorsal horns, intermediate zone and ventral horns of the rabbit's spinal cord were studied and compared with neurohistopathological changes. With the exception of PI concentration in the dorsal horns, ischemia of 25 min caused significant degradation of all phospholipids. While short-lasting recirculation (1 h) did not returned the levels of phospholipids to control values, postischemic recirculation for 3 h sharply increased the resynthesis of all phospholipids, but only the concentration of PE, PS, and PI in the dorsal horns and PC in the intermediate zone significantly improved and returned close to control values. Corresponding neurohistopathological changes resulting after the same reperfusion periods are given.
    No preview · Article · Sep 1998 · Neurochemical Research
  • N Lukácová · P Jalc · J Marsala
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    ABSTRACT: Changes in the concentration of membrane-bound phospholipids following single (25-min) spinal cord ischemia and 3 h of reperfusion were determined. These were compared with the changes following brief repeated (8-, 8-, and 9-min) ischemia followed each time by reperfusion for 1 h, or the same periods of ischemia followed by 8 h, 8 h, and 24 h of reperfusion, respectively. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), and sphingomyelin (SM) were assayed in regions of the spinal cord of the rabbit, including gray matter, white matter, dorsal horns, intermediate zone, and ventral horns. The brief repeated ischemia with 1-h reperfusions produced more extensive degradation of phospholipids in almost all regions compared with the equivalent time of ischemia (25 min) in a single period. After a lengthy reperfusion after repeated ischemia, the phospholipids were resynthesized with the exception of the phosphatidylinositol in the gray matter. The resynthesis was most pronounced in the dorsal horns and in the white matter.
    No preview · Article · Aug 1998 · Molecular and Chemical Neuropathology
  • N Lukácová · P Jalc · J Marsala
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    ABSTRACT: Degradation of membrane bound phospholipids in CNS during ischaemia begins with extreme rapidity. Sublethal ischaemia influences ischaemic tolerance in the affected neurons and is stressful enough to induce neuronal changes such as postischaemic hypoperfusion, transient suppression of protein synthesis and induction of stress (HSP) proteins. It seems, that the nature of factors responsible for ischaemic tolerance may involve the activation of multiple different systems. The aim of this study was to investigate the changes of phospholipids in gray matter regions of spinal cord following sublethal ischaemia repeated in long intervals of reperfusion. Male rabbits, weight range 2.5-3.5 kg were used in the experiment. They were divided in following groups : 1. control animals; 2. animals subjected to 25 min ischaemia; 3. animals subjected to 25 min ischaemia and 3 h of reperfusion; 4. animals subjected to sublethal (8-8-9 min) ischaemia repeated in long-lasting (8-8-24 h) intervals of reperfusion. Phospholipids were separated by thin layer chromatography, lipidic phosphorus was assessed spectrophotometrically. Sublethal ischaemia repeated in long-lasting intervals of reperfusion increased the concentration of phospholipids to control levels in all gray matter regions. The resynthesis in the dorsal horns, of PC and PE in the ventral horns and of PC in the intermediate zone. An excessive renewal of phospholipids after sublethal ischaemia repeated in longer intervals of reperfusion was most pronounced in the eh dorsal horns of the spinal cord and can be the result of many defensive cellular mechanisms.
    No preview · Article · Aug 1998 · Bratislavske lekarske listy
  • N. Lukacova · P. Jalc · J. Marsala

    No preview · Article · Jan 1998
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    A Fercáková · P Jalc
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    ABSTRACT: The influence of preischemic treatment with N-acetylcysteine (NAC) in a rat model of 30 min global brain ischemia was studied. Neuronal changes in is-chemic and preischemically NAC-treated animals after 3 days survival were evaluated by light and electron microscopy. Severe ischemic injury was found in the population of small B-cells showing heavy cytoplas-mic vacuolation. Preischemic administration of NAC substantially reduced the occurrence of degenerating small neurons. Morphological changes in trigeminal ganglion have been studied following a short period of focal ischemia due to 1-7 min compression of the ganglion in the cat (2) and rabbit (6). This procedure results in an ischemic and mechanical lesion of the rootlets and trigeminal ganglion cells. There is a groving body of evidence that formation of free radicals during is-chemia and early recirculation is involved in the de-velopment of reperfusion neuronal injury (3). It has been shown that using the free radical scavengers, such as superoxide dismutase, enhanced neuronal survival in some models of cerebral ischemia. Recently, it has been reported that N-acetylcystein (NAC) can amelio-rate brain damage after transient brain ischemia (4). In this study we evaluated the influence of preis-chemic NAC treatment on reperfusion alterations in trigeminal ganglion neurons after global brain is-chemia. Adult rats were subjected to 30 min global brain ischemia using the model of 4-vessel occlusion (7) followed by 3 days recirculation. In another group of animals a single dose of NAC (400 mg/kg i. p.) was administered 30 min before the onset of ischemia. After transcardial perfusion with 4% paraformalde-hyde trigeminal ganglia were removed and processed for electron microscopy. Light microscopic examination (1 fim sections) in ischemic animals with 3 days survival revealed an in-creased number of large A-type and small B-type chro-matolytic neurons with eccentric nuclei. Numerous B-cells showed heavy vacuolation (14.5%). In rats with NAC pretreatment the occurrence of vacuolated cells was significantly reduced (7.4%). Ultrastructural al-terations in A-cells manifested by perinuclear chroma-tolysis, dark nuclei, formation of vacuoles and myeli-noid bodies. The mitochondria were well preserved. Small B-cells were more severely affected showing signs of degeneration with an extensive mitochondrial vacuolation. Preischemic NAC treatment markedly re-duced the presence of degenerating B-cells in compar-ison with non-treated animals. It is supposed that the mechanism of protective influence of NAC lies in enhancing of glutathion scav-enging system (1). There are also other possibilities which may account for NAC neuroprotective effect. Ischemic/reperfusion injury substantially impaires mi-crovascular and endothelial function that may be ameliorated by NAC due to inhibition of endothelial and neutrophils interaction (5). The results obtained demonstrate that NAC administration appear to be an efficient intervention which can reduce ischemic al-terations of neurons in the both cell populations and may have a value in the treatment of some cerebrovas-cular diseases.
    Preview · Article · Jan 1998 · Chemical Papers- Slovak Academy of Sciences
  • A. Ferčáková · P. Jalč

    No preview · Article · Jan 1998