[Show abstract][Hide abstract] ABSTRACT: Through an improved enrichment protocol, a genomic library for (AC)12 repeats was constructed and 34 microsatellite loci were isolated and characterized in an endangered animal, Tibetan chicken,
Gallus gallus domesticus. In the 34 loci, ten loci showed a distinct allelic variation ranging from 4 to 14 alleles in 54 individuals tested. Polymorphism
information content (PIC) ranged from 0.590 to 0.869 with an average of 0.713. Average observed and expected heterozygosities
were 0.7988 (ranged from 0.310 to 1.000) and 0.7495 (ranged from 0.609 to 0.897), respectively. These ten microsatellites
loci would be the valuable genetic markers for further investigation of Tibetan chicken.
No preview · Article · Jun 2010 · Conservation Genetics
[Show abstract][Hide abstract] ABSTRACT: Eight polymorphic microsatellite markers were developed for the endangered Tibetan chicken (Gallus gallus domesticus) from a partial genomic library enriched for CAA repeat. Polymorphism of these loci was evaluated in 54 Tibetan chicken specimens.
The number of alleles per locus ranged from 3 to 14 and Polymorphism information content (PIC) ranged from 0.524 to 0.790
with an average of 0.635. These loci should provide useful molecular tools to study conservation genetics of the Tibetan chicken.
No preview · Article · Dec 2009 · Conservation Genetics Resources