[Show abstract][Hide abstract]ABSTRACT: To elucidate the relationship between apoptotic protease activating factor-1 (Apaf-1) gene and gastric cancer.
Thirty-five postoperative cancer and adjacent normal tissue samples were collected in the present study. Expression of the Apaf-1 gene in these samples was analyzed by semi-quantitative RT-PCR. Loss of heterozygosity (LOH) was used to determine whether there was loss of Apaf-1 gene in domain of 12q22-23 in the samples. Promoter methylation of Apaf-1 gene in the samples was analyzed by methylation specific (MSP) PCR.
The expression of Apaf-1 mRNA in gastric cancer tissue samples was 51%. The LOH frequency of D12S346, D12S1706, D12S327, D12S1657 and D12S393 was 33%, 8%, 58%, 12% and 42%, respectively. Fifty percent LOH was found at two sites and 17% LOH at three sites. Apaf-1 mRNA expression decreased significantly in 13 cases (rs=0.487, P=0.003). The rate of Apaf-1 promoter methylation was 49% in gastric cancer tissue samples and 23% in para-cancerous tissue samples. Promoter methylation occurred significantly in 16 of 18 gastric cancer tissue samples with decreased expression of Apaf-1 mRNA rs=0.886, P=10(-6)).
The expression of Apaf-1 gene is low in gastric cancer tissues. Methylation of Apaf-1 gene promoter and LOH in domain of 12q22-23 are the main reasons for the expression and altered expression of Apaf-1 gene.
Article · Nov 2007 · World Journal of Gastroenterology
[Show abstract][Hide abstract]ABSTRACT: To study the effects of magnolol and honokiol on isolated smooth muscle of gastrointestinal tract and their relationship with Ca2+, and on the gastric emptying and the intestinal propulsive activity in mice.
Routine experimental methods using isolated gastric fundus strips of rats and isolated ileum segments of guinea pigs were adopted to measure the smooth muscle tension. The effects of magnolol 10(-3), 10(-4), 10(-5) mol/L, and honokiol 10(-4), 10(-5), 10(-6) mol/L on the contractility of gastric fundus strips of rats and ileum of guinea pigs induced by acetylcholine (Ach) and 5-hydroxytryptamine (5-HT) was assessed respectively. The method using nuclein and pigment methylene blue was adopted to measure the gastric retention rate of nuclein and the intestinal propulsive ratio of a nutritional semi-solid meal for assessing the effect of magnolol and honokiol (0.5, 2, 20 mg/kg) on gastric emptying and intestinal propulsion.
Magnolol and honokiol significantly inhibited the contractility of isolated gastric fundus strips of rats treated with Ach or 5-HT and isolated ileum guinea pigs treated with Ach or CaCl2, and both of them behaved as non-competitive muscarinic antagonists. Magnolol and honokiol inhibited the contraction induced by Ach in Ca2+-free medium and extracellular Ca2+-dependent contraction induced by Ach. Each group of magnolol and honokiol experiments significantly decreased the residual rate of nuclein in the stomach and increased the intestinal propulsive ratio in mice.
The inhibitory effect of magnolol and honokiol on contractility of the smooth muscles of isolated gastric fundus strips of rats and isolated ileum of guinea pigs is associated with a calcium-antagonistic effect. Magnolol and honokiol can improve the gastric emptying of a semi-solid meal and intestinal propulsive activity in mice.
Article · Aug 2005 · World Journal of Gastroenterology
[Show abstract][Hide abstract]ABSTRACT: To investigate the resistance rate of Helicobacter pylori (H pylori ) to clarithromycin, metronidazole, amoxicillin and tetracycline to guide clinical practice, and to study the mechanism of H pylori resistant to clarithromycin.
Thirty H pylori strains were isolated from the mucosa of peptic ulcer, gastric tumor and chronic gastritis patients, then the minimal inhibitory concentration (MIC) to clarithromycin, metronidazole, amoxicillin and tetracycline was evaluated by E-test method. The sequence analysis of PCR fragments was conducted in 23S rRNA gene of H pylori resistant to clarithromycin to get the resistance mechanism of the bacteria.
Among 30 H pylori strains, 7 cases were resistant to clarithromycin, 12 to metronidazole, 2 to tetracycline and no strain was found to be resistant to amoxicillin. The resistance rates were 23.3%, 40%, 6.7% and 0%, respectively. Three new mutation points were found to be related to the clarithromycin resistance in H pylori isolates, which were G2224A, C2245T and T2289C.
In northeast China, H pylori shows high resistance to metronidazole, while sensitive to amoxicillin. The mechanism of resistance to clarithromycin may be related to the mutation of G2224A, C2245T and T2289C in the 23S rRNA gene.
Article · May 2004 · World Journal of Gastroenterology
[Show abstract][Hide abstract]ABSTRACT: To study the expression of apoptosis related gene survivin and its relation with expression of bcl-2, bax protein in epithelial ovarian cancer.
Expression of survivin gene was evaluated by reverse transcription polymerase chain reaction (RT-PCR) in 35 cases of epithelial ovarian cancer, and compared with that in 10 cases of borderline cancer, 10 cases of benign tumors and 10 cases of normal tissue. Expression of bcl-2 and bax was detected by immunohistochemistry streptomycin-avidin-biotin-peroxidase complex (SABC) method, and correlation between them was analyzed.
Expression of survivin gene was detected in a significantly greater proportion in epithelial ovarian cancer and borderline tumors than benign tumors and normal tissue. There was no relationship between survivin gene expression and FIGO stage, histologic grade, pathological type and lymphatic metastasis. Expression of bcl-2 and bax protein was positively and negatively correlated with expression of survivin gene respectively.
survivin gene may play an important role in pathogenesis of ovarian cancer, apoptosis related gene bcl-2 may have a synergic role and bax have an antagonistic role with survivin gene in formation and progression of ovarian cancer.
[Show abstract][Hide abstract]ABSTRACT: AIM:To study the prokinetic effect of Chinese herbs Zhuxiang powder (ZXP) on gastrointestinal motility mice and its mechanism. METHODS:The effects of ZXP on gastric emptying and intestinal propulsion were investigated in vivo by measuring gastric remainder rates and intestinal propulsion rates of Blue Dextron (BD) 2000. Based on the level of main effect of the orthogonal design list L16(2 15 ), the authors explored the mechanism of ZXP on gastric emptying by administering atropine intraperitoneally to mice 20 minutes prior to meal RESULTS:Compared with control group, the rates of gastric emptying and intestinal propulsion rates increased significantly in ZXP group (72.0± 12.4 % vs 63.7± 13.1 %,P Rhizoma, Atractylodis Macrocephalae. There were first-grade mutual roles within Rhizoma, Atractylodis Macrocephalae and Radix Aucklandiae.The prokinetic effect of Chinese herbs Zhu Xiang Powder (ZXP) on gastrointestinal motility mice could be inhibited partially by atropine, especially on gastric emptying. CONCLUSION:ZXP can accelerate the gastric emptying and intestinal propulsion in mice significantly. The main herbs in ZXP are radix Aucklandiae and Fructus Amomi. The mechanism of ZXP promoting gastrointestinal motility is related partially to the cholinergic nerve function. 结果:术香冲剂的胃排空率(72.0 ±12.4 % vs 63.7 ±13.1 %, P 白术>鸡内金, 白术与木香有交互作用.术香冲剂的促动力
[Show abstract][Hide abstract]ABSTRACT: AIM:To evaluate the effects of Chinese herbs Xiangsha Pingweisan (XSPWS) decoction and its component on gastric emptying in healthy mice. METHODS:Mice were randomly divided into control group, cisapride group and XSPWS group. The effects of XSPWS on gastric emptying were investigated in vivo by measuring gastric remainder rates of Blue Dextron (BD) 2000. Additionally, the effect of each component herb in XSPWS recipe was evaluated in the same way according to orthogonal design. RESULTS:Compared with control group ((100.0±24.2) %), the rates of gastric remainder in XSPWS group ((39.5±18.3) %) and cisapride group ((36.9±16.4) %) were all significantly decreased (P 0.05). Except radix glycyrrhizae preparta group, the gastric emptying rates of other component herbal groups were significantly higher than that of control group (P
[Show abstract][Hide abstract]ABSTRACT: AIM: To investigate the insulin resistance and serum growth hormone (GH) level in patients with liver cirrhosis. METHODS:Serum insulin and GH concentrations were de- tected by radioimmunoassay and insulin sensitivity index (ISI) were calculated in 40 patients with liver cirrhosis and 22 healthy controls. According to Child-Pugh classification, cirrhotic patients were divided into Child A (16 cases), Child B (13 cases) and Child C (11 cases) groups. Meanwhile the correlations between Child-Pugh classification and serum insulin levels, ISI or serum GH levels were analyzed. RESULTS:The serum insulin and GH levels in cirrhosis were higher significantly than those in healthy controls (17.8± 7.2 U/L vs 10.1± 3.4 U/L , P
Article · Oct 2002 · World Chinese Journal of Digestology