[Show abstract][Hide abstract] ABSTRACT: Ear size and shape are distinct conformation characteristics of pig breeds. Previously, we identified a significant quantitative trait locus (QTL) influencing ear surface on pig chromosome 5 in a White Duroc×Erhualian F2 resource population. This QTL explained more than 17% of the phenotypic variance.
Four new markers on pig chromosome 5 were genotyped across this F2 population. RT-PCR was performed to obtain expression profiles of different candidate genes in ear tissue. Standard association test, marker-assisted association test and F-drop test were applied to determine the effects of single nucleotide polymorphisms (SNP) on ear size. Three synthetic commercial lines were also used for the association test.
We refined the QTL to an 8.7-cM interval and identified three positional candidate genes i.e. HMGA2, SOX5 and PTHLH that are expressed in ear tissue. Seven SNP within these three candidate genes were selected and genotyped in the F2 population. Of the seven SNP, HMGA2 SNP (JF748727: g.2836 A>G) showed the strongest association with ear size in the standard association test and marker-assisted association test. With the F-drop test, F value decreased by more than 97% only when the genotypes of HMGA2 g.2836 A>G were included as a fixed effect. Furthermore, the significant association between g.2836 A>G and ear size was also demonstrated in the synthetic commercial Sutai pig line. The haplotype-based association test showed that the phenotypic variance explained by HMGA2 was similar to that explained by the QTL and at a much higher level than by SOX5. More interestingly, HMGA2 is also located within the dog orthologous chromosome region, which has been shown to be associated with ear type and size.
HMGA2 was the closest gene with a potential functional effect to the QTL or marker for ear size on chromosome 5. This study will contribute to identify the causative gene and mutation underlying this QTL.
Full-text · Article · Mar 2012 · Genetics Selection Evolution
[Show abstract][Hide abstract] ABSTRACT: The expression profiles of three candidate genes in ear tissue by RTPCR. Figure S1 shows the expression level of HMGA2, SOX5 and PTHLH in ear tissue obtained by RT-PCR.
[Show abstract][Hide abstract] ABSTRACT: Primers for QTL fine-mapping, gene expression analyses, and SNP identification and genotyping. Table S1 contains primer sequences, annealing temperatures and amplicons of the microsatellite markers, Table S2 primers for gene expression analyses, Table S3 sequences and assay conditions of the primers for SNP identification and Table S4 sequences and assay conditions of the primers for SNP genotyping.