José M. Gallardo

Spanish National Research Council, Madrid, Madrid, Spain

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Publications (127)250.92 Total impact

  • Dataset: pr0701963
    Mónica Carrera · Benito Cañas · Carmen Piñeiro · Jesús Vázquez · José Manuel Gallardo

    No preview · Dataset · Nov 2015
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    ABSTRACT: Dietary intervention with ω-3 marine fatty acids may potentially modulate inflammation and oxidative stress markers related with CVD, metabolic syndrome and cancer. The aim of this study was to evaluate whether different proportions of ω-3 EPA and DHA intake provoke a modulation of the production of lipid mediators and then, an influence on different indexes of inflammation and oxidative stress in a controlled dietary animal experiment using Wistar rats. For such scope, a lipidomic SPE-LC-ESI-MS/MS approach previously developed was applied to determine lipid mediators profile in plasma samples. The effect of ω-3 fatty acids associated to different ratios EPA:DHA was compared with the effect exerted by ω-3 ALA supplementation from linseed oil and ω-6 LA from soybean oil. CRP showed a tendency to greater inflammatory status in all ω-3-fed animals. Interestingly, ratios 1:1 and 2:1 EPA:DHA evidenced a noteworthy healthy effect generating a less oxidative environment and modulating LOX and COX activities toward a decrease in the production of proinflammatory ARA eicosanoids and oxidative stress biomarkers from EPA and DHA. In addition, the ability of 1:1 and 2:1 fish oil diets to reduce lipid mediator levels was in concurrence with the protective effect exerted by decreasing inflammatory markers as ω-6/ω-3 ratio in plasma and membranes. It was also highlighted the effect of a higher DHA amount in the diet reducing the healthy benefits described in terms of inflammation and oxidative stress. Results support the antiinflammatory and antioxidative role of fish oils and, particularly, the effect of adequate proportions EPA:DHA. Copyright © 2015. Published by Elsevier Inc.
    No preview · Article · Nov 2015 · The Journal of Nutritional Biochemistry
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    ABSTRACT: Proteome changes in the longissimus thoracis bovine muscle in response to pre-slaughter stress were assessed on the basis of two-dimensional electrophoresis (2-DE) data. In this study, the bootstrap resampling statistical technique and a new measure of relative change of the volume of 2-DE protein spots are shown to be more efficient than commonly used statistics to reliably quantify changes in protein abundance in stress response. The data are supplied in this article and are related to “Tackling proteome changes in the longissimus thoracis bovine muscle in response to pre-slaughter stress” by Franco et al. [1].
    Full-text · Article · May 2015
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    ABSTRACT: Unlabelled: Pre-slaughter stress has adverse effects on meat quality that can lead to the occurrence of Dark Firm Dry (DFD) meat in cattle. This study explores the previously uncharacterized proteome changes linked to pre-slaughter stress in the longissimus thoracis (LT) bovine muscle. Differential proteome profiles of DFD and normal (non-DFD) LT meat samples from male calves of the Rubia Gallega breed were assessed by 2-DE coupled to MS analysis (LC-MS/MS and MALDI TOF/TOF MS). A total of seven structural-contractile proteins (three different myosin light chain isoforms, two fast skeletal myosin light chain 2 isoforms, troponin C type 2 and cofilin-2) and three metabolism enzymes (triosephosphate isomerase, ATP synthase and beta-galactoside alpha-2,6-sialyltransferase) were found to have statistically significant differential abundance in sample groups. In addition, 2-DE in combination with the phosphoprotein-specific fluorescent dye Pro-Q DPS revealed that highly phosphorylated fast skeletal myosin regulatory light chain 2 isoforms underwent the most intense relative change in muscle conversion to DFD meat. Therefore, they appear to be the most sensitive biomarkers of stress just prior to slaughter in Rubia Gallega. Overall, these findings will facilitate a more integrative understanding of the biochemical processes associated with stress in cattle muscle and their effects in meat quality. Biological significance: Pre-slaughter stress is a crucial factor in meat production. Animals destined for slaughter are stressed by a variety of endogenous and exogenous factors that negatively affect the complex post-mortem biochemical events underlying the conversion of muscle into meat. The study of the muscle proteome has a great relevance for understanding the molecular mechanisms associated with stress. However, there is no information available on the molecular changes linked to pre-slaughter stress in cattle on the proteome scale. Our study led to the identification of a number of candidate proteins associated with the response to pre-slaughter stress in the LT bovine muscle of the Rubia Gallega breed. The functions of those significantly changed proteins have a clear biological relationship with stress response. These findings contribute to a deeper insight into the molecular pathways that respond to stress in cattle.
    Full-text · Article · Apr 2015 · Journal of proteomics
  • Ignacio Ortea · José M. Gallardo
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    ABSTRACT: Three factors defining the traceability of a food product are production method (wild or farmed), geographical origin and biological species, which have to be checked and guaranteed, not only in order to avoid mislabelling and commercial fraud, but also to address food safety issues and to comply with legal regulations. The aim of this study was to determine whether these three factors could be differentiated in shrimps using stable isotope ratio analysis of carbon and nitrogen and/or multi-element composition. Different multivariate statistics methods were applied to different data subsets in order to evaluate their performance in terms of classification or predictive ability. Although the success rates varied depending on the dataset used, the combination of both techniques allowed the correct classification of 100% of the samples according to their actual origin and method of production, and 93.5% according to biological species. Even though further studies including a larger number of samples in each group are needed in order to validate these findings, we can conclude that these methodologies should be considered for studies regarding seafood product authenticity.
    No preview · Article · Mar 2015 · Food Chemistry
  • Mónica Carrera · Benito Cañas · José M. Gallardo
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    ABSTRACT: Food fingerprint can be defined as a specific molecular marker diagnostic for a characteristic food/nutritional state or condition. In this chapter, we review the powerful and potential application of proteomics technologies for the discovery and validation of protein food fingerprints applied to food quality and authenticity challenges. For this purpose, two consecutive phases of the proteomics pipeline used in many laboratories (discovery and target-driven stages) are described in detail. The potentiality of this workflow is emphasized using different subjects concerning to food quality and safety. Food authentication, allergen detection, microorganism contamination, and quality changes during the storage and processing are the main topics where proteomics is being successfully applied. Finally, future directions and new potential perspectives are also provided.
    No preview · Chapter · Dec 2014

  • No preview · Chapter · Sep 2014
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    ABSTRACT: The present research draws a map of the characteristic carbonylation of proteins in rats fed high caloric diets with the aim of providing a new insight of the pathogenesis of metabolic diseases derived from the high consumption of fat and refined carbohydrates. Protein carbonylation was analyzed in plasma, liver and skeletal muscle of Sprague-Dawley rats fed a high-fat high-sucrose (HFHS) diet by a proteomics approach based on carbonyl-specific fluorescence-labelling, gel electrophoresis and mass spectrometry. Oxidized proteins along with specific sites of oxidative damage were identified and discussed to illustrate the consequences of protein oxidation. The results indicated that long-term HFHS consumption increased protein oxidation in plasma and liver, meanwhile protein carbonyls from skeletal muscle did not change. The increment of carbonylation by HFHS diet was singularly selective on specific target proteins: albumin from plasma and liver, and hepatic proteins such as mitochondrial carbamoyl-phosphate synthase (ammonia), mitochondrial aldehyde dehydrogenase, argininosuccinate synthetase, regucalcin, mitochondrial ATP synthase subunit beta, actin cytoplasmic 1, mitochondrial glutamate dehydrogenase 1. The possible consequences that these specific protein carbonylations have on the excessive weight gain, insulin resistance and nonalcoholic fatty liver disease resulting from HFHS diet consumption have been discussed.
    No preview · Article · Sep 2014 · The Journal of Nutritional Biochemistry
  • Manuel Pazos · Lucía Méndez · José M. Gallardo · Santiago P. Aubourg
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    ABSTRACT: The effect of high hydrostatic pressure (HHP) treatment (150, 300, and 450 MPa for 0, 2.5, and 5 min) on total sodium dodecyl sulfate (SDS)-soluble and sarcoplasmic proteins in frozen (−10 °C for 3 months) Atlantic mackerel (Scomber scombrus) was evaluated. Proteomics tools based on image analysis of SDS-polyacrylamide gel electrophoresis (SDS-PAGE) protein gels and protein identification by tandem mass spectrometry (MS/MS) were applied. Total SDS-soluble proteins, composed in high proportion of myofibrillar proteins, were stable under pressurization treatment in terms of solubility and electrophoretic gel profiles. However, pressurization reduced sarcoplasmic proteins’ solubility, modified their one-dimensional (1-D)/two-dimensional (2-D) SDS-PAGE patterns in a direct-dependent manner, and exerted a selective effect on particular sarcoplasmic proteins depending on processing conditions. Thus, protein bands assigned to creatine kinase, fructose-bisphosphate aldolase A, glycogen phosphorylase, and β-enolase were degraded at 300–450 MPa. Additionally, the stability of triosephosphate isomerase B, phosphoglucomutase, and phosphoglycerate kinase-1 was found to be HHP-reduced when submitted at 450 MPa. HHP processing (300–450 MPa) also induced a cross-linking product formation of pyruvate kinase and two compounds derived from tropomyosin at 450 MPa. Frozen storage time of pressurized samples induced an additional lessening in protein solubility, but electrophoretic patterns were not modified. The present investigation emphasizes the higher lability of sarcoplasmic proteins under HHP treatment and the important role of these proteins in the sensory quality enhancement provided by milder HHP conditions on frozen mackerel. HHP technology is expected to boost the development of novel tailored processing approaches to tackle food quality challenges.
    No preview · Article · Aug 2014 · Food and Bioprocess Technology
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    Full-text · Conference Paper · Jun 2014
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    ABSTRACT: The study innovatively pinpoints target proteins of carbonylation, a key PTM induced by oxidative stress, in the SHROB (genetically obese spontaneously hypertensive) rat model of metabolic syndrome (MetS). Protein carbonylation was assessed by a fluorescence-labelling proteomics approach, and complemented with biometric and biochemical markers of MetS. SHROB and healthy Wistar rats were fed two diets, soybean and linseed oil supplementations, in order to distinguish intrinsic carbonylation of SHROB animals from diet-modulated carbonylation unrelated to MetS. First exploratory data showed similar carbonylation patterns and metabolic conditions in SHROB rats fed soybean and linseed, but different from Wistar animals. A total of 18 carbonylated spots in liver, and 12 in skeletal tissue, related to pathways of lipid (29.6%), carbohydrate (25.9%) and amino acid (18.5%) metabolisms, were identified. In particular, SHROB animals present higher carbonylation in four liver proteins belonging to lipid metabolism, redox regulation and chaperone activity (ALDH2, PDI, PDIA3, PECR), and in the skeletal muscle ALDOA involved in muscle dysfunction. Conversely, SHROB rats display lower carbonylation in liver albumin, AKR1C9, ADH1 and catalase. This investigation provides a novel perspective of carbonylation in the context of metabolic disorders, and may be a starting point to characterize new redox pathways exacerbating MetS. Oxidative stress is a concomitant factor in the pathogenesis of MetS that induces oxidative PTM as carbonylation. Through the use a redox proteomics approach, we have thoroughly mapped the occurrence of protein targets of carbonylation in the genetically-induced MetS model SHROB rat. The present research brings a new insight of MetS pathogenesis and it may provide valuable information to understand the biological impact of oxidative stress in patients with MetS.
    No preview · Article · Apr 2014 · Journal of proteomics
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    ABSTRACT: The present work describes the development of a robust and sensitive targeted analysis platform for the simultaneous quantification in blood plasma of lipid oxygenated mediators and fatty acids using solid-phase extraction (SPE) and high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS). The concurrent analysis of these lipid mediators is challenging because of their instability, differences in solubility, and the frequent occurrence of isobaric forms with similar fragmentation patterns. Results demonstrated that the reduction of SPE temperature to 4 °C is a critical parameter for preserving the hydroperoxy derivatives. Polymeric HLB cartridges increased 40–50 % ARA, EPA, and DHA sensitivity compared to C18 sorbent and also provided higher global performance for most hydroxides and other oxidation products. The proposed method for the two tested mass analyzers yields high sensitivity, good linearity, and reproducibility, with detection limits ranging 0.002–7 ng/mL and global recoveries as high as 85–112 %. However, the additional advantage of the linear ion trap (LIT) mass analyzer working in full scan product ion mode, compared to the triple quadrupole (QqQ) operating in multiple reaction monitoring (MRM), should be noted: the full scan product ion mode provides the full fragmentation spectra of compounds that allowed the discrimination of coeluting isomers and false positive identifications without additional chromatography development. The proposed lipidomic procedure demonstrates a confident, simple, and sensitive method to profile in plasma a wide range of lipid eicosanoid and docosanoid mediators, including innovatively the analysis of hydroperoxy congeners and nonoxidized PUFA precursors. Figure ᅟ
    No preview · Article · Mar 2014 · Analytical and Bioanalytical Chemistry
  • Mónica Carrera · Benito Cañas · José M. Gallardo

    No preview · Chapter · Jan 2014
  • José Manuel Gallardo · Ignacio Ortea · Mónica Carrera
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    ABSTRACT: This review is a critical overview of advances in proteomics applied in food technology, which may be classified into two main topics: (i) authentication of food components as a tool to comply with foodlabeling regulations and policies; and, (ii) food-technology research, mainly for the development of fast, reliable methods to detect and to identify spoilage and/or pathogenic microorganisms in food and for the study of changes in food components as a consequence of food processing. (c) 2013 Elsevier Ltd. All rights reserved.
    No preview · Article · Dec 2013 · TrAC Trends in Analytical Chemistry
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    ABSTRACT: This work focuses on the effect of a previous high pressure processing (HPP) on the lipid damage development occurring during the frozen storage (−10°C; up to 3 months) of Atlantic horse mackerel (Trachurus trachurus). HPP conditions included different pressure (150, 300, 450 MPa) and pressure holding time (0.0, 2.5, 5.0 min) values. During frozen storage, horse mackerel muscle was analyzed for lipid hydrolysis (free fatty acid assessment; FFA) and oxidation (formation of peroxides, thiobarbituric acid reactive substances, and fluorescent compounds), and polyene content. An inhibition of lipid hydrolysis development was observed; thus, both an increasing pressure level and pressure holding time led to a marked inhibition of FFA content throughout the frozen storage. Concerning the lipid oxidation development, a partial inhibition was also obtained during the frozen storage (months 1 and 3) by increasing the pressure level applied (namely, fluorescent and peroxide compound formation); however, pressure holding time did not lead to a definite trend. No effect of HPP treatment was concluded on the polyene content of the fish muscle lipids. Present research provides novel information concerning the employment of HPP technology focused on the inhibition of lipid damage during a subsequent frozen storage.Practical applications: Frozen storage of fatty and medium-fat fish species is known to be strongly limited by lipid damage development, which is a drawback to its commercialization as such or to its subsequent employment as raw material in other kinds of processing (canneries, smoking, etc.). Present research provides valuable information concerning the employment of the high pressure technology to inhibit lipid damage development during the subsequent frozen storage of Atlantic horse mackerel (T. trachurus). Thus, both an increasing pressure level (from 150 to 450 MPa) and pressure holding time (from 0 to 5 min) led to a marked inhibition of lipid hydrolysis during frozen storage. Additionally, inhibition of lipid oxidation was produced by increasing the pressure level. Since the response to HPP of marine species has been reported to vary with species, a preliminary study is recommended to be carried out before applying the high pressure-frozen storage combining strategy.
    No preview · Article · Dec 2013 · European Journal of Lipid Science and Technology
  • Mónica Carrera · Benito Cañas · José M. Gallardo
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    ABSTRACT: A comprehensive overview of the application of proteomics for the assessment of quality and safety in fishery products is presented in this review. For this purpose, two consecutive phases of the proteomics pipeline used in our laboratory (discovery and target-driven stages), are described in detail. The potentiality of this workflow is emphasized using different subjects concerning fish quality and safety. Fish authentication, allergen detection, microorganism contamination and quality changes during storage and processing are the main topics where proteomics is being successful applied. Future directions and new potential perspectives are also provided.
    No preview · Article · Nov 2013 · Food Research International
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    ABSTRACT: High consumption of fish carries a lower risk of cardiovascular disease as a consequence of dietary omega-3 long chain polyunsaturated fatty acid (n-3 PUFA; especially EPA and DHA) content. A controversy exists about the component/s responsible of these beneficial effects and, in consequence, which is the best proportion between both fatty acids. We sought to determine, in healthy Wistar rats, the proportions of EPA and DHA that would induce beneficial effects on biomarkers of oxidative stress, and cardiovascular disease risk. Female Wistar rats were fed for 13 weeks with 5 different dietary supplements of oils; 3 derived from fish (EPA/DHA ratios of 1:1, 2:1, 1:2) plus soybean and linseed as controls. The activities of major antioxidant enzymes (SOD, CAT, GPX, and GR) were determined in erythrocytes and liver, and the ORAC test was used to determine the antioxidant capacity in plasma. Also measured were: C reactive protein (CRP), endothelial dysfunction (sVCAM and sICAM), prothrombotic activity (PAI-1), lipid profile (triglycerides, cholesterol, HDLc, LDLc, Apo-A1, and Apo-B100), glycated haemoglobin and lipid peroxidation (LDL-ox and MDA values). After three months of nutritional intervention, we observed statistically significant differences in the ApoB100/ApoA1 ratio, glycated haemoglobin, VCAM-1, SOD and GPx in erythrocytes, ORAC values and LDL-ox. Supplementation with fish oil derived omega-3 PUFA increased VCAM-1, LDL-ox and plasma antioxidant capacity (ORAC). Conversely, the ApoB100/ApoA1 ratio and percentage glycated haemoglobin decreased. Our results showed that a diet of a 1:1 ratio of EPA/DHA improved many of the oxidative stress parameters (SOD and GPx in erythrocytes), plasma antioxidant capacity (ORAC) and cardiovascular risk factors (glycated haemoglobin) relative to the other diets.
    Full-text · Article · Oct 2013 · Lipids in Health and Disease
  • José M. Gallardo · Mónica Carrera · Ignacio Ortea
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    ABSTRACT: The close relationship between nutrition and health has led to changes in the habits of consumers, who are demanding products that meet their dietary and nutritional preferences. In light of this demand, both academia and food industries face a new challenge: the need for developing strategies and products that are not only safe, but also contribute to the maintenance of good health and that can even prevent the development of specific diseases. Advances in proteome analysis offer multiple applications that can be used to meet the challenge. These applications can be divided into three main topics: (i) quality and food safety control; (ii) the study of changes in food components as a consequence of food processing; and (iii) nutritional aspects and characterization of new food ingredients with beneficial effects on human health. In this chapter, we illustrate how proteomic tools can be applied to these three main food science topics.
    No preview · Chapter · Mar 2013
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    ABSTRACT: The present study aims to compare two molecular technologies, 16S rRNA sequencing and matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI TOF - MS), for bacterial species identification in seafood. With this aim, 70 reference strains from culture collections, including important seafood-borne pathogenic and spoilage bacterial species, and 50 strains isolated from commercial seafood products, were analyzed by both techniques. Genomic analysis only identified the species of 50% of the isolated strains, proving to be particularly poor at identifying members of the Pseudomonas and Bacillus genera. In contrast, MALDI-TOF MS fingerprinting identified 76% of the strains at the species level. The mass spectral data were submitted to the SpectraBank database (, making this information available to other researchers. Furthermore, cluster analysis of the peak mass lists was carried out with the web application SPECLUST and the calculated groupings were consistent with results determined by a phylogenetic approach that is based on the 16S rRNA sequences. However, the MALDI-TOF MS analysis demonstrated more discriminating potential that allowed for better classification, especially for the Pseudomonas and Bacillus genera. This is of importance with respect to the varying pathogenic and spoilage character at the intra-genus and intra-species level. In this sense, MALDI-TOF MS demonstrated to be a competent bacterial typing tool that extends phenotypic and genotypic approaches, allowing a more ample classification of bacterial strains.
    No preview · Article · Mar 2013 · Electrophoresis
  • Mónica Carrera · Benito Cañas · José M. Gallardo

    No preview · Chapter · Jan 2013

Publication Stats

2k Citations
250.92 Total Impact Points


  • 1999-2015
    • Spanish National Research Council
      • • Department of Food Science & Technology
      • • Instituto de Investigaciones Marinas de Vigo
      • • Department of Chemical and Surfactants Technology
      Madrid, Madrid, Spain
  • 2009-2013
    • Marine Environmental Research Institute
      Blue Hills, Connecticut, United States
  • 2012
    • University of Santiago de Compostela
      • Faculty of Veterinary
      Santiago, Galicia, Spain
  • 2007
    • Complutense University of Madrid
      Madrid, Madrid, Spain