Li Li

Western Sydney University, Sydney, New South Wales, Australia

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Publications (22)45.47 Total impact

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    ABSTRACT: Eighteen polycyclic aromatic hydrocarbons (PAHs) in PM2.5 collected near the Nanjing Olympic Sports Center across the Asian Youth Games (AYG) period (from August 2 to August 28, 2013) were analyzed using GC–MS. Their levels, sources and health risks to human were discussed. Results showed that the total concentrations of PAHs in PM2.5 were 9.43, 7.21 and 8.83 ng m− 3 for pre- (August 3–15), during- (August 16–24) and post- (August 25–28) AYG periods, respectively. They were dominated by 5-ring and 6-ring PAHs. Total PAHs concentrations in PM2.5 during AYG period decreased by 24%, when compared with those for pre-AYG period. For combustion-derived PAHs and carcinogenic PAHs, they decreased by 26% and 21%, respectively. It implied that the pollution control measures implemented during the AYG can effectively reduce the emission of PAHs from various sources. The poor correlations between PAHs and meteorological parameters also favored that the variations of PAHs were raised by the changes of emission sources. Diagnostic ratios and principal component analysis revealed that vehicle emission and coal combustion were the predominant contributors, with minimal effects from biomass burning and petroleum. The health risks for human exposed to PAHs in PM2.5 were quantitatively assessed by BaP equivalent concentration (BaPeq) and the incremental lifetime cancer risk (ILCR). The estimated ILCR value of PAHs during the AYG periods decreased by 23% and 27% for children and adults when compared with those for the pre-AYG, respectively. It indicated that the pollution control measures reduced the risks of PAHs to sportsmen or human gathered around the Olympic Sport Center.
    No preview · Article · Jan 2016
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    ABSTRACT: A Schiff base type ligand with the combination of the julolidine and the quinoline groups has been reported as a potential chemosensor in detecting the cobalt(II) ion among other heavy and transition metal ions in solution. However, no crystal structure of such a ligand with any metal ions has been reported. In this work, its complexation with 3d transition metal ions (Mn(II), Co(II), Ni(II), Cu(II) and Zn(II)) has been investigated with five new complexes being synthesised, and spectroscopically and structurally characterised. [Mn2L2(CH3OH)2(CH3COO)2]·CH3OH (1) {HL (C22H21N3O) = ((E)-9-((quinolin-8-ylimino)methyl)-1,2,3,5,6,7-hexahydropyrido[3,2,1-ij]quinolin-8-ol)} shows a dinuclear structure with two Mn:L:acetate (1:1:1) units bridged by two methanol molecules. [CoL2(NO3)]·CH3OH·H2O (2) and [NiL2]·H2O (3) exhibit mononuclear structures with a Co:L or Ni:L ratio of 1:2. [CuL(CH3COO)]·1/3CH3OH (4) demonstrates a mononuclear structure and the Cu ion has a square planar coordination polyhedron with a L ligand and a highly non-symmetrical acetate anion. [Zn2L2(CH3COO)2]·CH3OH (5) has two types of dinuclear units, both with two ZnL units bridged by two acetate anions but in three different bridging coordination modes. Their vibrational modes, absorption and photoluminescence properties have also been investigated.
    No preview · Article · Dec 2015
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    ABSTRACT: Mast cells and basophils are effector cells in the pathophysiology of allergic diseases. Targeted elimination of these cells may be a promising strategy for the treatment of allergic disorders. Our present study aims at targeted delivery of anti-FcεRIα Fab-conjugated celastrol-loaded micelles toward FcεRIα receptors expressed on mast cells and basophils to have enhanced anti-allergic effect. To achieve this aim, we prepared celastrol-loaded (PEO-block-PPO-block-PEO, Pluronic) polymeric nanomicelles using thin-film hydration method. The anti-FcεRIα Fab Fragment was then conjugated to carboxyl groups on drug-loaded micelles via EDC amidation reaction. The anti-FcεRIα Fab-conjugated celastrol-loaded micelles revealed uniform particle size (93.43 ± 12.93 nm) with high loading percentage (21.2 ± 1.5% w/w). The image of micelles showed oval and rod like. The anti-FcεRIα Fab-conjugated micelles demonstrated enhanced cellular uptake and cytotoxity toward target KU812 cells than non-conjugated micelles in vitro . Furthermore, diffusion of the drug into the cells allowed an efficient induction of cell apoptosis. In mouse model of allergic asthma, treatment with anti-FcεRIα Fab-conjugated micelles increased lung accumulation of micelles, and significantly reduced OVA-sIgE, histamine and Th2 cytokines (IL-4, IL-5, TNF-α) levels, eosinophils infiltration and mucus production. In addition, in mouse model of passive cutaneous anaphylaxis, anti-FcεRIα Fab-conjugated celastrol-loaded micelles treatment significantly decreased extravasated evan's in the ear. These results indicate that anti-FcεRIα Fab-conjugated celastrol-loaded micelles can target and selectively kill mast cells and basophils which express FcεRIα, and may be efficient reagents for the treatment of allergic disorders and mast cell related diseases.
    No preview · Article · Dec 2015 · Journal of Biomedical Nanotechnology
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    ABSTRACT: Intensive construction activities worsened the surrounding atmospheric environment in China. Eighteen polycyclic aromatic hydrocarbons (PAHs) in fine particles (PM2.5) were collected at a subway construction site (SC) of Nanjing and compared with a regional background mountainous site (BM) to examine the influence of anthropogenic activities on concentrations, sources and health risks of PAHs. Average PAH concentrations at SC were higher than BM at a factor of about 5.9. All PAH species at SC were higher than BM, with the SC/BM ratios ranging from 1.3 (NaP) to 10.3 (BaP). PAH profiles differed for the two sites. The SC site had higher mass fractions of PAHs from coal combustion and vehicle emission, while the BM site held higher mass percentages of PAHs from long-range transported wood combustion and industrial activities. Lower temperature at BM may lead to the higher mass percentages of low ring PAHs. Coal combustion, traffic emissions and biomass burning were the common sources for PAHs at both SC and BM. Construction workers were exposed to higher BaPeq concentrations, nearly ten times of the background site and their lifetime cancer risk reached to 0.6 per 1,000,000 exposed worker, owing to the influence of coal combustion, vehicle emission and industrial activities at the surroundings of SC.
    No preview · Article · Aug 2015 · Atmospheric Research
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    ABSTRACT: A large discrete face-capped tetranuclear iron(II) cage, [Fe4L4](BF4)(8)center dot n(solvent), was synthesised via metal-ion directed self-assembly. The cage is formed from a rigid tritopic ligand that incorporates chelating imidazole-imine functional groups. The cage displays temperature induced spin-crossover and LIESST effects and is amongst the largest iron(II) tetrahedral cages with such properties reported. The synthesis, structure and magnetic properties of this new metallo-cage are presented.
    Full-text · Article · May 2015
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    ABSTRACT: The in situ Schiff base condensation of 2-acetylpyrazine with tris(2-aminoethyl)amine in the presence of zinc(II) perchlorate was carried out in absolute ethanol and 95 % ethanol, respectively. Two new tripodal metalloligands, 1 and 2, were isolated. The formation of complexes 1 and 2 has been verified by NMR, mass spectral studies and X-ray (for 2), with the evidence indicating that a zinc ion is incorporated in the tripodal cavity defined by the tren backbone in each case. However the products differed in the number of Schiff base condensation reactions that had occurred. While the use of absolute ethanol resulted in condensation at all three primary amine sites of tris(2-aminoethyl)amine, employing 95 % ethanol yielded condensation at only one of the primary amine sites. These different outcomes can be ascribed, at least in part, to the effect of the different water contents in the respective reaction solvents resulting in a shift of the dynamic equilibrium involving imine formation towards the precursor amine and ketone reagents. In 1, steric considerations dictate that the three uncoordinated pyrazine nitrogen donors will have their coordination vectors oriented in a mutually divergent manner suitable for coordination to three different metal centres when acting as a metalloligand while for 2, the X-ray structure confirms that the single uncoordinated (pendent) pyrazine nitrogen is also oriented for ready coordination to a second metal centre.
    No preview · Article · May 2015 · Journal of inclusion phenomena and macrocyclic chemistry
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    ABSTRACT: The focus on this mini-review is on the use of metalloligands for the construction of discrete self-assembled, polyhedral metallo-cages. This metalloligand approach employs metal bound multifunctional ligand building blocks that display predetermined, well-defined electronic and structural preferences that facilitate the rational design and construction of both homo- and heteronuclear cages. The assembled cages exhibit a variety of defined topologies that, for example, range from trigonal bipyramidal to rhombododecahedral.
    Full-text · Article · May 2015 · Journal of inclusion phenomena and macrocyclic chemistry
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    ABSTRACT: Daily PM2.5 samples were collected at a suburban site of Nanjing around 2014 Chinese Spring Festival (SF) and analyzed for 18 kinds of polycyclic aromatic hydrocarbons (PAHs) by GC-MS. Comparison of PAH concentrations during different periods, with different air mass origins and under different pollution situations was done. Sources were analyzed by diagnostics ratios and principal component analysis (PCA). The threat of PAHs was assessed by BaP equivalent concentrations (BaPeq) and incremental lifetime cancer risk (ILCR). The averaged PAHs for pre-SF, SF and after SF periods were 50.6, 17.2 and 29ngm-3, indicating the variations of PAH sources, with reduced traffic, industrial and construction activities during SF and gradually re-starting of them after-SF. According to PAH mass concentrations, their relative abundance to particles, ratio of PAHs (3-ring+4-ring)/PAHs(5-ring+6-ring), mass concentrations of combustion-derived and carcinogenic PAHs, fireworks burning is an important source for PAHs during SF. The ILCR values for Chinese New Year day were 0.68 and 3.3 per 100,000 exposed children and adults. It suggested the necessity of controlling fireworks burning during Chinese SF period which was always companied with serious regional haze pollution. PAH concentrations exhibited decreasing trend when air masses coming from the following directions as North China Plain (63.9ngm-3)>Central China (53.0ngm-3)>Shandong Peninsula (46.6ngm-3)>Northwest China (18.8ngm-3)>Sea (15.8ngm-3). For different pollution situations, they decreased as haze (44.5ngm-3)>fog-haze (28.4ngm-3)>clear (12.2ngm-3)>fog day (9.2ng m-3). Coal combustion, traffic emission, industrial processes and petroleum (only for non-SF holiday periodss) were the main sources of PM2.5 associated PAHs. Fireworks burning contributed 14.0% of PAHs during SF period. Directly measurement of PAHs from fireworks burning is urgently needed for source apportionment studies in the future.
    No preview · Article · Mar 2015 · Science of The Total Environment
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    ABSTRACT: Previous studies showed that the Ca(2+)-activated Cl(-) channel (CaCC) was involved in the pathogenesis of mucus hypersecretion induced by Interleukin-13 (IL-13). However, the mechanisms underlying the process were unknown. Recently, transmembrane protein 16A (TMEM16A) was identified as the channel underlying the CACC current. The aim of the current study was to investigate whether the TMEM16A channel is part of the mechanism underlying IL-13-induced mucus hypersecretion. We observed that both TMEM16A mRNA and protein expression were significantly up-regulated after treatment with IL-13 in human bronchial epithelial 16(HBE 16) cells, which correlated with an increase in mucus production. Additionally, mucus hypersecretion in rat airways was induced by intratracheal instillation of IL-13 and similar increases were observed in the expression of TMEM16A mRNA and protein in the bronchial epithelium. Niflumic acid (NA), a selective antagonist of CaCC, markedly blocked IL-13-induced mucin (MUC) 5AC mRNA and protein production in vivo and in vitro. Further investigation with HBE16 cells revealed that TMEM16A overexpression clearly promoted mucus production, IκBα phosphorylation, and p65 accumulation in the nucleus. The loss of TMEM16A resulted in inhibition of mucus production, and the TMEM16A-mediated production of MUC5AC was significantly blocked by a nuclear factor-kappa B (NF-κB) inhibitor. Therefore, the TMEM16A channel acts upstream of NF-κB in the regulation of mucus production. This is the first demonstration that the TMEM16A-NF-κB pathway is positively involved in IL-13-induced mucus production, which provides novel insight into the molecular mechanism of mucin overproduction. Copyright © 2015. Published by Elsevier Inc.
    No preview · Article · Mar 2015 · Experimental Cell Research
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    ABSTRACT: To investigate the vertical variation of water soluble inorganic ions (WSI) in aerosols at a regional background mountainous site, nine size fractions of particles (10.0–9.0, 9.0–5.8, 5.8–4.7, 4.7–3.3, 3.3–2.1, 2.1–1.1, 1.1–0.65, 0.65–0.43 and <0.43 μm) were collected at two different altitudes simultaneously at Huangshan in southeast China, from 14 September to 26 October of 2012. The mass concentrations of PM1.1, PM2.1 and PM10 were 17.07, 21.28 and 39.25 μg/m3 at the summit (SM, 1840 m), respectively and were 24.79, 29.02 and 42.39 μg/m3 at a lower height site (LL, 869 m). The average mass concentrations of total WSI for PM1.1, PM2.1 and PM10 were 9.59, 11.73 and 17.16 μg/m3 at SM, and were 16.88, 19.38 and 27.61 μg/m3 at LL. The concentrations of particulates and WSI both decreased with altitude increasing from 869 m to 1840 m. SO42− and NH4+ exhibited peak values 0.43–0.65 μm at SM, whereas maintained peak values 0.65–1.1 μm at LL. NO3− were mostly concentrated in fine mode for SM but in coarse mode for LL. Further analyses showed that at LL, the heterogeneous reaction on coarse particles containing more calcium and magnesium may explain the higher concentrations of NO3− in coarse mode and also the higher temperature may reduce the concentrations of NO3− in fine mode. Na+, Cl− and K+ exhibited bimodal size distributions; Ca2+ and Mg2+ showed maximum values in coarse mode. Aerosol acidity analysis showed a higher acidity of aerosol particles at LL when compared with those at SM. The average concentration of [H+] was relatively low when compared with those observed at two other mountains in China. This corresponds with the relatively low concentrations of SO42− and HSO4− and lower water content at Huangshan.
    Full-text · Article · Dec 2014 · Atmospheric Environment
  • Liang Yuan · Yan Yin · Qi Li · Hui Xiao · Li Li
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    ABSTRACT: To provide scientific support for improving air quality and for the policy-making during the Youth Olympic Games, the characteristics of aerosol concentration variation was analyzed with the data obtained during August in Nanjing, 2013. And further analysis was carried out to find the relationship between visibility and aerosol mass concentration as well as relative humidity (RH). The results show that the average mass concentration of PM10 and PM2.5 are 67.44 μg x m(-3) and 31.92 μg x m(-3), respectively. Integrated particle number and volume concentrations with diameter between 0.1 and 10 μm are 2 466.25 cm(-3) and 19.89 μm3 x cm(-3), respectively. A log-normal size distribution with 10-based logarithm applied to fit the measured aerosol size distributions shows two models with median diameters of 0.19 μm and 1.12 μm. Aerosol concentration in Nanjing is mainly affected by local source. The diurnal pattern of PM10 is influenced by the traffic density and the activity of boundary layer, while the daily variation of PM2.5 is slightly different because of the effects of traffic density and the formation of secondary pollutants during photochemical process. Statistical analysis on visibility reveals that visibility decreases with the increasing aerosol concentration and RH, PM2.5 concentration dominates the change of visibility when RH is less than 70%, while RH contributes more on the change for RH in excess of 70%.
    No preview · Article · Nov 2014 · Huan jing ke xue= Huanjing kexue / [bian ji, Zhongguo ke xue yuan huan jing ke xue wei yuan hui "Huan jing ke xue" bian ji wei yuan hui.]
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    Dataset: paper 4

    Full-text · Dataset · Oct 2014
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    Dataset: paper 4

    Full-text · Dataset · Oct 2014
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    ABSTRACT: Background Human cells release nano-sized vesicles called exosomes, containing mRNA, miRNA and specific proteins. Exosomes from one cell can be taken up by another cell, which is a recently discovered cell-to-cell communication mechanism. Also, exosomes can be taken up by different types of cancer cells, but the potential functional effects of mast cell exosomes on tumor cells remain unknown.Methods and resultsExosomes were isolated from the human mast cell line, HMC-1, and uptake of PKH67-labelled exosomes by the lung epithelial cell line, A549, was examined using flow cytometry and fluorescence microscopy. The RNA cargo of the exosomes was analyzed with a Bioanalyzer and absence or presence of the c-KIT mRNA was determined by RT-PCR. The cell proliferation was determined in a BrdU incorporation assay, and proteins in the KIT-SCF signaling pathway were detected by Western blot. Our result demonstrates that exosomes from mast cells can be taken up by lung cancer cells. Furthermore, HMC exosomes contain and transfer KIT protein, but not the c-KIT mRNA to A549 cells and subsequently activate KIT-SCF signal transduction, which increase cyclin D1 expression and accelerate the proliferation in the human lung adenocarcinoma cells.Conclusions Our results indicate that exosomes can transfer KIT as a protein to tumor cells, which can affect recipient cell signaling events through receptor-ligand interactions.
    Full-text · Article · Oct 2014 · Cell Communication and Signaling
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    ABSTRACT: An intensive sampling campaign was conducted in the autumn of 2012 to study the size distribution of inorganic ions and ion deposition at an offshore site of Yangtze River Delta region (YRD), China. Particles in < 0.43, 0.43–0.65, 0.65–1.1, 1.1–2.1, 2.1–3.3, 3.3–4.7, 4.7–5.8, 5.8–9.0 and 9.0–10.0 μm were collected and analyzed by ion chromatography for NH4+, Na+, Mg2 +, K+, Ca2 +, F−, Cl−, NO3− and SO42 −. The average mass concentrations in the nine particle sizes ranged from 13.1 to 38.7 μg m− 3, accounting for 5.5%–16.8% of the total mass. Concentrations exhibit bi-modal distribution, peaking at 0.65–1.1 μm and 3.3–4.7 μm. PM1.1, PM2.1 and PM2.1–10 account for 41%, 56% and 44% of PM10 implying the dominance of finer particles. Different ions hold different size distribution patterns. The concentrations of NO3−, SO42 −, NH4+, K+ and Ca2 + exhibit the highest values when compared to literature values indicating the serious air pollution situation in YRD. A haze–precipitation–fog transition course is captured. The precipitation can decrease all the particles in the nine sizes by 20%–62% and the fog formation process can promote the particles' accumulation, with their mass concentrations increasing by 26%–232% except for those in 5.8–9.0 μm and 9.0–10 μm. Mass percentages of SO42 −, NO3− and NH4+ exhibit decreases during precipitation and increases during fog formation course. The anion to cation ratios are lower than 1.0 indicating the anion deficiency and they exhibit significant negative relationship with the particle diameter. The Cl-dep (%) is higher for particles larger than 0.65 μm, increasing from 1.3% to 61% with size added. Sea salt only accounts for 0.12%–2.1% of PM10. The ratios of nss-SO42 −/NO3− decrease with increasing particle size, from 1.68 to 0.69, and are well fit as linear form (r = − 0.74, p = 0.02). It indicates that stationary sources make more contributions to smaller particles and vehicle emission is more important for larger particles. The dry deposition fluxes for F−, Cl−, NO3− and SO42 − are 3.10, 0.59, 22.99 and 17.04 mg/(m2 d). They exhibit multi-linear correlation with corresponding ion concentrations and the coefficients are higher for coarse particles. More researches are needed to obtain the distribution, transportation and deposition characteristic of ions at Chinese coastal area.
    Full-text · Article · Oct 2014 · Atmospheric Research
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    Hui Xiao · Juan Wang · Yanan Liu · Li Li
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    ABSTRACT: c-Kit and epidermal growth factor receptor (EGFR) have critical roles in cell proliferation and differentiation in patients with non-small cell lung cancer (NSCLC). The present study aimed to investigate the prognostic impact of c-Kit and/or EGFR expression in tumor tissue samples from 146 patients with NSCLC. c-Kit expression was analyzed using immunohistochemistry and the expression of EGFR was assessed using fluorescence in situ hybridization. Univariate and multivariate analyses identified that c-Kit is a significant negative prognostic factor. The expression of c-Kit was correlated with poor differentiation, pleura involvement and smoking history (P=0.043, 0.007 and 0.032, respectively). Furthermore, patients with c-Kit-positive expression were associated with a significantly lower overall survival compared with those exhibiting c-Kit-negative expression (P=0.048). The median follow-up time was 19 months post-surgery. EGFR gene amplification as a result of polysomy of chromosome 7 was found to be negatively correlated with poor differentiation and smoking history (P=0.023 and 0.044, respectively). The findings of the present study indicate that c-Kit and EGFR expression is a strong, independent, negative prognostic factor in NSCLC.
    Preview · Article · Aug 2014 · Oncology letters
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    ABSTRACT: We established a novel gene expression analysis platform, Multiplex Competitive RT-PCR Using Fluorescent Universal Primers (MCF-PCR), to study multi-gene expression patterns simultaneously. This platform combines fluorescent universal primers, multiplex competitive RT-PCR, and capillary electrophoretic separation, which ensures MCF-PCR a reliable, medium-throughput, cost-effective technology for gene expression profiling. With cloned standard DNAs, the detection limits, precision, and sensitivity of MCF-PCR were evaluated and compared with that of the assay without adding competitive templates and real-time PCR, respectively. The results showed that detection limit was 3.125 × 10(3) to 3.2 × 10(6) copies, and 10 % copy differences between two samples can be detected by MCF-PCR. To validate MCF-PCR, we analyzed expression profile of five genes in interleukin (IL)-4/IL-13 pathway in peripheral blood of 20 healthy adults and 20 allergic dermatitis patients; three genes including IL-4, IL-13, and STAT6 were found differentially expressed in the two sample groups, which maybe key players in IL-4/IL-13 immunological signaling pathway and need further function analysis.
    No preview · Article · Dec 2012 · Analytical and Bioanalytical Chemistry
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    ABSTRACT: Celastrol has been found to be a potent anti-inflammatory and antitumor plant derivative recently. Herein we established an accurate reverse phase HPLC (RP-HPLC) determination method of celastrol, and prepared an effective nanoscale drug delivery system from the optimized formulations of celastrol-loaded carboxyl functioned poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol)(PEO-block-PPO-block-PEO, Pluronic) polymeric nanomicelles. Rotatable central composite design (RCCD) and response surface methodology (RSM) were applied to improve the celastrol entrapment efficiency (EE), drug loading percentage (DLP), and decrease the particle size. The characteristics of the optimized micelles including particle size distribution, morphology, zeta potential and in vitro release of celastrol from micelles were carried out. Results showed that RP-HPLC method was successfully applied to detect and qualify the celastrol. The drug quantification range of the method was 20-200 microg/mL with a linear correlation coefficient of greater than 0.999. The average accuracy (99.763%), the precision (0.521%) and recovery (99.63%) for this method were good. The optimal conditions for the preparation of celastrol-loaded polymeric nanomicelles were found to be: the celastrol/polymer weight ratio 8.5-9 mg/25 mg, hydration volume 11-17 mL. Under the optimal conditions, the EE was 100.3 +/- 4.3%, DLP was 22.8 +/- 1.0%, the average particle size was 117.3 +/- 1.27 nm and the zeta potential was -2.19 +/- 0.15 mV. Transmission electron micrograph (TEM) showed the micelles to be oval and rodlike shaped, with mean diameter around 20 nm. The in vitro experiments proved that celastrol in polymeric nanomicelles released gradually over the period of 24 h. These results showed that the RCCD and RSM could efficiently be applied for optimized preparation of celastrol-loaded polymeric nanomicelles.
    No preview · Article · Jun 2012 · Journal of Biomedical Nanotechnology
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    ABSTRACT: The immunoglobulin E (IgE) high-affinity receptor FcεRI expressed on mast cells and basophils plays a critical role in triggering allergic disease. The co-aggregation of the FcεRI and FcγRIIb receptors is inhibitory to FcεRI signaling and holds great potential for the treatment of IgE-mediated allergies. In China, Dermatophagoides farinae is a common anaphylaxis trigger. Therefore, in this study, the FcγRIIb-mediated immunomodulating activity of recombinant Fcγ-Der f2 fusion protein was tested in a Der f2-allergic murine model. Following the treatment, bronchoalveolar lavage fluid (BALF) was collected to measure the expression of several Th1/Th2-type cytokines (IL-5, TNF-α, IL-12p70, IL-4, IL-10, IFN-γ and IL-18) and histamine, while blood was used to detect the specific IgE and IgG-types anti-Der f2 antibodies, for measurement. In contrast to the saline-treated allergic mice, the levels of Der f2-specific IgE, cytokines and histamine were lowered in the Fcγ-Der f2-treated allergic mice, in addition to the rare inflammatory cell infiltration in the airways and blood vessels revealed by histopathological examination. The recombinant Fcγ-Der f2 protein was demonstrated to function as an effective immunotherapeutic agent, suggesting that chimeric human Fcγ-allergen proteins could be used in the development of antigen-specific immunotherapy for human allergic diseases.
    No preview · Article · Apr 2012 · Immunologic Research
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    ABSTRACT: The high-affinity receptor for immunoglobulin E (IgE) plays a central role in allergy diseases. Previous studies have reported the association of variants in the proximal promoter of FCER1A with IgE levels as well as allergy disorders. Another promoter gene polymorphism that is located upstream of exon 1 has not been investigated. We investigated the association of variants in the promoter located upstream of FCER1A exon 1 with serum IgE levels and allergy diseases in a Han Chinese population. A total of 97 patients with atopic dermatitis (AD), 123 patients with chronic urticaria (CU), 286 children with asthma, and control groups were screened for polymorphisms in the promoter region located upstream of FCER1A exon 1 by the polymerase chain reaction-ligation detection reaction method. Total serum IgE levels were tested in groups. The rare allele A of the rs2427837 A/G polymorphism was significantly different in the AD group compared with the controls. No association with the polymorphism was observed in the CU group. In asthmatic patients, IgE levels were higher in the mutation genotypes GA of rs2427837 and TC of rs2251746 compared with normal genotype individuals. The minor allele of rs2427837 and rs2251746 in FCER1A is a genetic risk factor of high IgE levels.
    Preview · Article · Mar 2012 · Human immunology

Publication Stats

60 Citations
45.47 Total Impact Points

Institutions

  • 2015
    • Western Sydney University
      • School of Science and Health
      Sydney, New South Wales, Australia
    • Chongqing Medical University
      Ch’ung-ch’ing-shih, Chongqing Shi, China
  • 2014-2015
    • Nanjing University of Information Science & Technology
      Nan-ching, Jiangsu Sheng, China
  • 2011-2015
    • Shanghai Jiao Tong University
      • Department of Laboratory Medicine
      Shanghai, Shanghai Shi, China
  • 2012
    • Renji Hospital
      Shanghai, Shanghai Shi, China