[Show abstract][Hide abstract] ABSTRACT: Pigs play a critical role in Japanese encephalitis virus (JEV) transmission between mosquitos and humans. In 2009, lots of piglets developed symptom of viral encephalitis in a pig farm in Yunchen, Shanxi province.
Virus isolation was carried out in BHK-21 cells. Immunohistochemistry, RT-PCR and indirect immunofluorescent assay were used to identify the newly isolated virus. The complete genome of one isolate (SX09S-01 strain) was sequenced and analyzed. Two phylogenetic trees were constructed on the basis of the 24 full-length JEV genomes and 62 E genes mostly selected from China.
JEV SX09S-01 strain was isolated from piglets. Sequence analysis indicates that the completed genome sequences of this strain consists of 10965 nucleotides and there are 13 nucleotides deletion in the 3' nontranslated variable region. Compared with other JEV strains, homology ranges from 99.1% (XJ69) to 74.1% (XZ0934) and 99.6% (XJ69) to 91.1% (XZ0934) on the level of nucleotide and amino acid sequences, respectively. Phylogenetic trees show that SX09S-01 strain belongs to genotype I and it is most closely related to the XJ69 strain.
Genotype I of JEV still circulates in Yuncheng and it is thus important for active surveillance on genotype I of JEV from the swine population.
[Show abstract][Hide abstract] ABSTRACT: Comparisons of the complete genomic sequence of the SX09S-01 strain with the sequences of 23 JEV strains available in GenBank. 1-24: SX09S-01, Ishikawa, KV1899, XJ69, FU, CJN-L1, CJN-S1, GP78, HW, JaGAr 01, JEV-AT31, Ling, Nakayama, P3, SA14-14-2, SA14, SH0601, T1P1, Vellore P20778, WHe, Beijing-1, JKT6468, Muar, XZ0934. The percent nucleotide sequence identities of the complete genomes are presented at the upper right. The percent amino acid sequence identities of the complete genomes are shown in the lower left. The percentage of SX09S-01 sequence identities are indicated in boldface type.
[Show abstract][Hide abstract] ABSTRACT: Sources of the JEV strains used in the phylogenetic analysis in this study. a Information unavailable. b Strain names labeled with bold world represent the full-length genome sequences available in GenBank. c Sequences used in the present study.
[Show abstract][Hide abstract] ABSTRACT: Comparative analysis of neurovirulence and neuroinvasiveness of the newly swine isolate and P3 strain. a Examined by intracerebral inoculation with 30 μl. b Examined by intraperitoneal inoculation with 500 μl.