Publications (18)

  • [Show abstract] [Hide abstract] ABSTRACT: Oxidative stress has been implicated in cardiac hypertrophy and heart failure. Vascular peroxidase 1 (VPO1), a peroxidase in the cardiovascular system, utilizes the hydrogen peroxide (H2O2) derived from co-expressed NADPH oxidases (NOX) to produce hypochlorous acid (HOCl) and catalyze peroxidative reactions. Our previous studies showed that VPO1 contributes to the vascular smooth muscle cell proliferation and endothelial dysfunction in spontaneous hypertensive rats (SHRs), however, the role of VPO1 in cardiomyocytes hypertrophy is still uninvestigated. The present study was therefore undertaken to examine the role of VPO1 in the angiotensin II induced cardiac hypertrophy and the underlying mechanism by which VPO1 regulates the redox signaling. As compared to WKY rats, the SHRs exhibited increased myocyte cross sectional area, enhanced Nox2 and VPO1 expression level in cardiac tissue, and an increased Ang II level in plasma. In cultured H9c2 cell line, Ang II increased the hypertrophy related gene (BNP/ANF) expression and the cellular surface area, which was attenuated by knocking down of VPO1 via VPO1 siRNA or pharmacological inhibition of NOX/VPO1 pathway. Moreover, the enhanced hypochlorous acid (HOCl) production and phosphorylation of ERK1/2 was suppressed by VPO1 knockdown. Furthermore, the protective role of VPO1 siRNA transfection on H9c2 cardiomyocytes hypertrophy was abrogated upon the HOCl stimulation, and the phosphorylated ERK1/2 expression level was found also up-regulated following HOCl stimulation. In conclusion, these results suggest that the Nox2/VPO1/HOCl/ERK1/2 redox signaling pathway was implicated in the pathogenesis of Ang II induced cardiac hypertrophy.
    Article · Aug 2016
  • Haiyang Peng · Luyao Chen · Xiao Huang · [...] · Ruizheng Shi
    [Show abstract] [Hide abstract] ABSTRACT: Asymmetric dimethylarginine (ADMA), the endogenous inhibitor of nitric oxide synthase (NOS), contributes to endothelial dysfunction and subsequent cardiovascular events including hypertension. Vascular peroxidase 1 (VPO1) is a novel heme-containing peroxidase that utilizes hydrogen peroxide (H2O2) generated from co-expressed nicotinamide adenine dinucleotide phosphate (NADPH) oxidase to catalyze peroxidative reactions. Our previous study revealed a clear connection between VPO1 gene expression and endothelial dysfunction in spontaneously hypertensive rats. In the present study, we explored whether VPO1 participates in endothelial dysfunction during hypertension by increasing ADMA production. Spontaneously hypertensive rats displayed impaired endothelium-dependent relaxation, decreased eNOS expression and nitric oxide production, significantly increased VPO1 expression in both plasma and aorta tissue, and an increased ADMA level in plasma. In cultured endothelial cells, angiotensin II increased the ADMA level by inhibiting dimethylarginine dimethylaminohydrolase activity, which was inhibited by knockdown of VPO1 using small hairpin RNA. Moreover, the NADPH oxidase inhibitor, and the hydrogen peroxide scavenger attenuated angiotensin II-mediated up-regulation of VPO1 and generation of hypochlorous acid. Furthermore, VPO1-derived hypochlorous acid suppressed recombinant dimethylarginine dimethylaminohydrolase activity and increased ADMA production. VPO1 plays a critical role in ADMA production via H2O2–VPO1– hypochlorous acid pathways, which may contribute to endothelial dysfunction in hypertension.
    Article · Jul 2016 · Journal of the American Society of Hypertension (JASH)
  • Luyao Chen · Shushan Zhao · Guangjie Cheng · [...] · Guogang Zhang
    [Show abstract] [Hide abstract] ABSTRACT: Objective: To assess the association between myeloperoxidase (MPO) gene polymorphism and coronary artery disease (CAD). Methods: Several databases were used to retrieve relevant literature up to March 2013 by keywords. A Meta-analysis was performed by Stata12.0 software to estimate the pooled odds ratio (OR) and the 95% confidence interval (CI). Heterogeneity among studies was tested and sensitivity analysis was applied. Publication bias was examined using Begg's funnel plot and Egger's linear regression test. Results: A total of 17 studies were included in this Meta-analysis. For MPO -463 G/A polymorphism, the pooled OR of A allele vs G allele was 0.58 [95% CI (0.47-0.72)] and the pooled OR of genotypes AA+AG vs GG was 0.58 [95% CI (0.46-0.72)]. In subgroup analysis of study population, AA and AG genotypes were significantly associated with CAD in Asians but not in Europeans. The MPO -463 G/A polymorphism in the stable angina pectoris subgroup was evaluated in 3 studies and the pooled OR of A allele vs G allele and genotypes AA+AG vs GG for proven CAD was 0.45 [95% CI (0.15-1.37)] and 0.57 [95% CI (0.19- 1.65)]. For MPO -129 A/G gene polymorphism, the pooled OR of genotype GG vs AA+AG was 0.91 [95% CI (0.74-1.10)]. Conclusion: A allele of MPO -463 G/A gene is associated with decreased risk of CAD except in the Europeans. There is no association between MPO -129 A/G gene polymorphisms and CAD risk.
    Article · Mar 2014 · Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
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    [Show abstract] [Hide abstract] ABSTRACT: Bronchoalveolar lavage (BAL) galactomannan (GM) assay has been used for diagnosing invasive aspergillosis (IA). We aimed to derive a definitive estimate of the overall accuracy of BAL-GM for diagnosing IA. We undertook a systematic review of thirty diagnostic studies that evaluated the BAL-GM assay for diagnosing IA. PubMed and CBM (China Biological Medicine Database) databases were searched for relevant studies published in all languages up until Feb 2012. The pooled diagnostic odds ratio (DOR) and summary receiver operating characteristic (SROC) were constructed for each cutoff value. Additionally, pooled sensitivity (SEN), specificity (SPE), and positive and negative likelihood ratios (PLR and NLR, respectively) were calculated for summarizing overall test performance. Thirty studies were included in this meta-analysis. The summary estimates of pooled DOR, SEN, SPE, PLR, and NLR of the BAL-GM assay (cutoff value 0.5) for proven or probable IA were 52.7 (95% confidence interval (CI) 31.8-87.3), 0.87 (95% CI 0.79-0.92), 0.89 (95% CI 0.85-0.92), 8.0 (95% CI 5.7-11.1) and 0.15 (95% CI 0.10-0.23) respectively. The SROC was 0.94 (95% CI 0.92-0.96). Compared with cutoff value of 0.5, it has higher DOR, SPE and PLR, and similar SEN and NLR with cutoff value of 1.0, which indicated the optimal cutoff value might be 1.0. Compared with BAL-GM, serum GM has a lower SEN and higher SPE, while PCR displays a lower SEN and a similar SPE. With the optimal cutoff value of 1.0, the BAL-GM assay has higher SEN compared to PCR and serum GM test. It is a useful adjunct in the diagnosis of proven and probable IA.
    Full-text Article · Aug 2012 · PLoS ONE
  • Dataset: Table S2
    [Show abstract] [Hide abstract] ABSTRACT: The correspondence between numbers and the studies. (XLSX)
    Dataset · Aug 2012
  • Dataset: Table S1
    [Show abstract] [Hide abstract] ABSTRACT: Detail characteristics of studies included in the Meta-analysis of diagnosis of IA using BAL-GM. (XLSX)
    Dataset · Aug 2012
  • Dataset: Table S3
    [Show abstract] [Hide abstract] ABSTRACT: Detail information of multiple univariable meta-regression and subgroup analyses. (XLSX)
    Dataset · Aug 2012
  • Dataset: Figure S7
    [Show abstract] [Hide abstract] ABSTRACT: Forest plots of sensitivity and specificity of serum GM and BAL-GM test for diagnosing proven or probable Invasive Aspergillosis. (TIF)
    Dataset · Aug 2012
  • Dataset: Figure S8
    [Show abstract] [Hide abstract] ABSTRACT: Forest plots of sensitivity and specificity of serum GM and BAL-GM test for diagnosing proven Invasive Aspergillosis. (TIF)
    Dataset · Aug 2012
  • Dataset: Figure S6
    [Show abstract] [Hide abstract] ABSTRACT: Likelihood ratio scattergram. (TIF)
    Dataset · Aug 2012
  • Dataset: Figure S2
    [Show abstract] [Hide abstract] ABSTRACT: Graphical depiction of residual-based goodness-of-fit, bivariate normality, influence and outlier detection analyses. (TIF)
    Dataset · Aug 2012
  • Dataset: Figure S4
    [Show abstract] [Hide abstract] ABSTRACT: Hierarchical summary ROC curve with confidence and prediction regions around mean operating sensitivity and specificity point. (TIF)
    Dataset · Aug 2012
  • Dataset: Figure S10
    [Show abstract] [Hide abstract] ABSTRACT: Forest plots of sensitivity and specificity of PCR assay and BAL-GM test for diagnosing proven or probable Invasive Aspergillosis. (TIF)
    Dataset · Aug 2012
  • Dataset: Figure S1
    [Show abstract] [Hide abstract] ABSTRACT: Paired forest plot depiction of empirical Bayes predicted versus observed sensitivity and specificity. (TIF)
    Dataset · Aug 2012
  • Dataset: Figure S5
    [Show abstract] [Hide abstract] ABSTRACT: Probability Modifying Plot. (TIF)
    Dataset · Aug 2012
  • Dataset: Figure S3
    [Show abstract] [Hide abstract] ABSTRACT: Bivariate box plot. (TIF)
    Dataset · Aug 2012
  • Dataset: Figure S9
    [Show abstract] [Hide abstract] ABSTRACT: The summary ROC curve of serum GM and BAL-GM test for diagnosing proven Invasive Aspergillosis. (TIF)
    Dataset · Aug 2012
  • [Show abstract] [Hide abstract] ABSTRACT: Parkinson's disease (PD) is one of the most common neurodegenerative diseases. Several studies had researched the association between the PITX3 gene polymorphism and Parkinson's disease. However, the results were inconsistent. To evaluate whether PITX3 gene polymorphism is involved in the risk of PD we conducted this meta-analysis. All the eligible studies were searched from the databases of Cochrane Central Register of Controlled Trials (CENTRAL) in The Cochrane Library, MEDLINE, EMBASE, and Science Citation Index EXPANDED in any languages up to May 2011. Finally ten studies about PITX3 gene including 5172 patients and 7290 controls were identified for meta-analysis. Meta-analysis was carried out to evaluate whether PITX3 gene polymorphism was associated with PD, and subgroup analysis was also performed when necessary. This meta-analysis finds that rs4919621 allele A was significantly associated with PD in the Caucasian population (P=0.04,). Subgroup analysis of early onset PD (EOPD) and late onset PD (LOPD) revealed that the rs2281983 allele C and rs4919621 allele A were significantly associated with the risk of PD (all of the P values were ≤ 0.0001) in EOPD population. This research indicated that the presence of the rs4919621 allele A significantly increased the risk of PD patients in Caucasian population while rs2281983 allele C and rs4919621 allele A were both risk factors in EOPD.
    Article · Mar 2012 · Journal of the neurological sciences