[Show abstract][Hide abstract]ABSTRACT: To assess the association between glutathione-S-transferase gene polymorphisms GSTT1, GSTM1 and GSTP1 and onset of azoospermia.
Multi-PCR was used to detect GSTM1 and GSTT1 gene deletions. Polymorphisms of GSTP1 were determined with restriction fragment length polymorphism (RFLP) method in 236 azoospermia patients and 142 healthy fertile male controls.
The frequency of M1 (-/-) and P1 (Ile/Val or Val/Val) genotype was 24.65% in the control group, which was significantly higher than that of the patient group (15.68%, P=0.031). Frequency of M1 (-/-), T1 (+/+) and P1 (Ile/Val or Val/Val) genotype was 12.68% in the control group, which was significantly higher than that of the patient group (5.51%, P=0.014).
The M1(-/-) and P1(Ile/Val or Val/Val) genotype and the M1(-/-), T1(+/+) and P1 (Ile/Val or Val/Val) genotype are associated with reduced risk of azoospermia in ethnic Chinese Han population.
Article · Feb 2013 · Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics
[Show abstract][Hide abstract]ABSTRACT: An expressed sequence tag of grass carp leukocyte cell-derived chemotaxin 2 (LECT2) gene was screened from an established intestinal cDNA library. Rapid amplification of cDNA ends gave rise to a full-length LECT2 cDNA (gcLECT2) with a complete open-reading frame of 474 bp, encoding 158 amino acids about 17.9 kDa. Homology search and sequence alignment showed that this deduced protein sequence shared a high identity with LECT2 from other vertebrates. Western blotting indicated immunological cross-reactivity occurs between grass carp and human LECT2 protein. This gcLECT2 genomic sequence is 1,868 bp in size, which consists of five exons and four introns. Real-time quantitative PCR analysis revealed that gcLECT2 gene is ubiquitously expressed in different tissues of healthy grass carp including brain, gut, liver, spleen, kidney, muscle and heart, while the expression levels were significantly increased in liver and spleen followed by Aeromonas salmonicida infection. 992 bp 5'-flanking region sequence was cloned and analyzed, where one CAAT box and one GC island were found. Our results showed that the LECT2 is suggested to be most possibly involved in the grass carp's immune response.
Article · Nov 2012 · Fish Physiology and Biochemistry
[Show abstract][Hide abstract]ABSTRACT: An enrichment culture method was applied to the isolation of a bacterial strain responsible for biodegradation of methidathion residues, from a methidathion-treated orchard. The strain (SPL-2) was identified as Serratia sp. according to its physiological characteristics and 16S rRNA gene phylogenetic analysis. Serratia sp. was able to grow in a poor medium consisting of mineral salts and using methidathion as the sole carbon source at a concentrations of 50–150 mg/L. The effects of multifactors on degradation of methidathion in pure cultures by Serratia sp. were investigated using an orthogonal experimental design L9 (34). On the basis of range analysis and ANOVA results, the most significant factors were temperature and inoculum size. The optimal conditions for methidathion biodegradation in pure cultures were a temperature in 30 °C, an inoculum size of 10 %, pH = 7 and an aeration rate of 200 rpm. Two different concentrations of strain SPL-2 fermenting liquids (OD600 = 0.2 and OD600 = 0.4) were prepared and applied to remove methidathion residues from agricultural products, and this process can be described by a first order rate model. In contrast to controls, the DT50 of methidathion was shortened by 35.7 %, 8.2 % and by 62.3 %, 57.5 % on OD600 = 0.2 and OD600 = 0.4 treated haricot beans and peaches, respectively. These results suggest that the isolated bacterial strain may have potential for use in bioremediation of methidathion-contaminated crops.
[Show abstract][Hide abstract]ABSTRACT: To investigate the prevalence and subtypes of microdeletions in azoospermia factor (AZF) region in infertile men from Sichuan in order to correlate genotypes with phenotypes.
Multiplex-PCR was used to detect sequence tagged sites (STS) of AZF microdeletions in 1011 infertile men including 713 cases of non-obstructive azoospermia and 298 cases of severe oligospermia.
The overall prevalence of microdeletions was 10.48% (106/1011), and the deletion rates were 11.08% (79/713) in non-obstructive azoospermia and 9.06% (27/298) in severe oligospermia. Complete AZFa or AZFb deletions were associated with azoospermia, whereas AZFc deletion (60.38%) was the most frequent deletion. The deletions were associated with variable spermatogenic phenotypes, and 37.50% of the patients with a deletion had sperms in the ejaculate. A mild decline in sperm concentration was found in two cases with partial AZFb deletion and one case with partial AZFb-c deletion.
Deletions of the AZFc region were most commonly found in our patients. All cases with complete AZFa or AZFb deletions and a proportion of cases with AZFc deletion were associated with azoospermia. Our study has provided more insight into the genotype-phenotype correlation, and confirmed that Yq microdeletion screening has a significant value for the diagnosis for male infertility.
Article · Apr 2012 · Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics
[Show abstract][Hide abstract]ABSTRACT: To investigate the frequency and type of both chromosomal abnormalities and Y chromosome microdeletions and analyze their association with defective spermatogenesis in Chinese infertile men.
This is a single center study. Karyotyping using G-banding and screening for Y chromosome microdeletion by multiplex polymerase chain reaction(PCR)were performed in 200 controls and 1,333 infertile men, including 945 patients with non-obstructive azoospermia and 388 patients with severe oligozoospermia.
Out of 1,333 infertile patients, 154(11.55%) presented chromosomal abnormalities. Of these, 139 of 945 (14.71%) were from the azoospermic and 15 of 388 (3.87%) from the severe oligozoospermic patient groups. The incidence of sex chromosomal abnormalities in men with azoospermia was 11.53% compared with 1.03% in men with severe oligozoospermia (P < 0.01). Also 144 of 1,333(10.80%) patients presented Y chromosome microdeletions. The incidence of azoospermia factor(AZF) microdeletion was 11.75% and 8.51% in patients with azoospermia and severe oligozoospermia respectively. Deletion of AZFc was the most common and deletions in AZFa or AZFab or AZFabc were found in azoospermic men. In addition, 34 patients had chromosomal abnormalities among the 144 patients with Y chromosome microdeletions. No chromosomal abnormality and microdeletion in AZF region were detected in controls.
There was a high incidence (19.80%) of chromosomal abnormalities and Y chromosomal microdeletions in Chinese infertile males with azoospermia or severe oligozoospermia. These findings strongly suggest that genetic screening should be advised to infertile men before starting assisted reproductive treatments.
Article · Mar 2012 · Journal of Assisted Reproduction and Genetics
[Show abstract][Hide abstract]ABSTRACT: In this study, spent mushroom (SM) (Tricholoma lobayense) was used as a new low-cost adsorbent for removing Congo Red (CR) from aqueous solutions in a batch process at 25°C. By varying the adsorbent dose, initial concentration, contact time, initial pH and particle size, the respective effects of these factors on the adsorption performance were explored. The sorption equilibrium data fitted Langmuir isotherm and the maximum adsorption capacity was 147.1 mg/g at 25°C. The kinetic data obtained at different initial concentrations were analyzed using pseudo-first-order, pseudo-second-order and intraparticle diffusion equations. The pseudo-second-order described the adsorption of Congo Red on spent mushroom very well.
Article · Mar 2011 · Desalination and water treatment