Chongxi Liu

China Agricultural University, Peping, Beijing, China

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Publications (56)116.99 Total impact

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    ABSTRACT: A novel actinomycete, designated strain NEAU-G9T, was isolated from the root of Polygonatum odoratum (Mill.), collected from Harbin, Heilongjiang province, north China and characterized using a polyphasic approach. Key morphological and chemotaxonomic properties confirmed the affiliation of strain NEAU-G9T to the genus Streptomyces. Strain NEAU-G9T contained LL-diaminopimelic acid as the diamino acid. The predominant menaquinones were MK-9(H8), MK-9(H6) and MK-9(H4). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The predominant fatty acids were iso-C16:0, anteiso-C15:0 and C16:0. 16S rRNA gene sequence similarity studies showed that strain NEAU-G9T belongs to the genus Streptomyces, and exhibits the highest sequence similarity to Streptomyces yanglinensis JCM 13275T (97.75 %). Whereas, phylogenetic analysis based on the 16S rRNA gene sequence indicated that it is most closely related to Streptomyces misakiensis JCM 4062T (97.12 % sequence similarity). A combination of DNA-DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-G9T can be clearly distinguished from S. yanglinensis JCM 13275T and S. misakiensis JCM 4062T. Consequently, strain NEAU-G9T represents a novel species of the genus Streptomyces, for which the name Streptomyces polygonati sp. nov. is proposed. The type strain is NEAU-G9T (=CGMCC 4.7237T=DSM 100521T).
    No preview · Article · Jan 2016 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: Three novel actinobacteria, designated strains NEAU-FSHN1T, NEAU-hd-3T and NEAU-Y6T, were isolated from a stream base, soil adjacent to the stream and a root of Corydalis yanhusuo L, respectively, collected from Wuchang, Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The three strains were observed to form scant aerial hyphae that differentiated into spherical spore vesicles. The phylogenetic analysis based on the 16S rRNA gene sequences of strains NEAU-FHSN1T, NEAU-hd-3T and NEAU-Y6T showed that the three novel isolates exhibit 99.2 % (NEAU-FHSN1T/NEAU-hd-3T), 99.2 % (NEAU-FHSN1T/NEAU-Y6T) and 99.7 % (NEAU-hd-3T/NEAU-Y6T) 16S rRNA gene sequence similarities with each other and that they are closely related to strains Streptosporangium shengliense NEAU-GH7T (sequence similarities 98.72, 98.85, 98.99 %), Streptosporangium roseum DSM 43021T (98.65, 98.51, 98.58 %) and Streptosporangium album DSM 43023T (98.41, 98.96, 98.89 %). However, the DNA–DNA hybridization values between strains NEAU-FSHN1T, NEAU-hd-3T and NEAU-Y6T were 61.2 % (NEAU-FSHN1T/NEAU-hd-3T), 63.5 % (NEAU-FHSN1T/NEAU-Y6T) and 65.8 % (NEAU-hd-3T/NEAU-Y6T), and the values between the three strains and their close phylogenetic relatives were also below 70 %. With reference to phenotypic characteristics, phylogenetic data and DNA–DNA hybridization results, the three strains can be distinguished from each other and their close phylogenetic relatives. Thus, strains NEAU-FHSN1T, NEAU-hd-3T and NEAU-Y6T are concluded to represent three novel species of the genus Streptosporangium, for which the names Streptosporangium lutulentum sp. nov., Streptosporangium fenghuangense sp. nov. and Streptosporangium corydalis sp. nov. are proposed. The type strains are NEAU-FHSN1T (=CGMCC 4.7141T = DSM 46740T), NEAU-Y6T (=CGMCC 4.7150T = DSM 46722T) and NEAU-hd3T (CGMCC 4.7212T = JCM 30058T), respectively.
    No preview · Article · Jan 2016 · Antonie van Leeuwenhoek
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    ABSTRACT: A novel actinobacteria, designated strain NEAU-ZJC8T, was isolated from a saline-alkaline soil collected from Heilongjiang Province, Northeast China and characterized using a polyphasic approach. Strain NEAU-ZJC8T exhibited morphological, cultural and chemotaxonomic features consistent with its classification in the genus Streptomyces. Growth occurred at 16-35 oC, pH 5.0-11.0 and in the presence of 0-11 % (w/v) NaCl. The cell wall peptidoglycan contained LL-diaminopimelic acid and glycine. Whole-cell hydrolysates mainly contained glucose, galactose and ribose. Predominant menaquinones were MK-9(H6), MK-9(H4) and MK-9(H8). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and two unidentified phospholipids. The major cellular fatty acids (> 10 %) were iso-C16:0, iso-C15:0, anteiso-C17:0 and C16:0. The G+C content of the DNA was 72.7 mol %. A phylogenetic tree based on 16S rRNA sequences showed that strain NEAU-ZJC8T formed a distinct clade within the genus Streptomyces and was closely related to Streptomyces haliclonae DSM 41970T (98.56 % similarity) and Streptomyces marinus NBRC 105047T (97.96 % similarity). A combination of DNA-DNA hybridization result and some phenotypic characteristics demonstrated that strain NEAU-ZJC8T could be distinguished from its closest phylogenetic relatives. Therefore, strain NEAU-ZJC8T represents a novel species of the genus Streptomyces, for which the name Streptomyces daqingensis sp. nov. is proposed. The type strain is NEAU-ZJC8T (=CGMCC 4.7178T =JCM 30057T).
    No preview · Article · Jan 2016 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: A novel actinomycete, designated strain 1C-HV12T, was isolated from the cuticle of Camponotus japonicas Mayr and characterized using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Promicromonospora and forms a monophyletic clade with its closest relatives Promicromonospora umidemergens JCM 17975T(99.17 % 16S rRNA gene sequence similarity), Promicromonospora vindobonensis V45T (98.88 %) and Promicromonospora iranensis HM 792T (98.85 %). Moreover, morphological and chemotaxonomic properties of strain 1C-HV12T were also confirmed the affiliation of the isolate to the genus Promicromonospora. The peptidoglycan hydrolysates contained alanine, glutamic acid and lysine and whole-cell sugars were galactose, glucose, rhamnose and ribose. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified phosphoglycolipid, an unidentified phospholipid and an unidentified glycolipid. The major menaquinones were MK-9(H4) and MK-9(H2). The predominant cellular fatty acids were anteiso-C15:0, iso-C15:0 and anteiso-C17:0. The DNA G+C content was 71.6 mol%. However, the low level of DNA-DNA relatedness and some phenotypic characteristics allowed the isolate to be differentiated from its closely related species. Therefore, it is proposed that strain 1C-HV12T represents a novel species of the genus Promicromonospora, for which the name Promicromonospora alba sp. nov. is proposed. The type strain is 1C-HV12T (=CGMCC 4.7283T=DSM 100490T).
    No preview · Article · Jan 2016 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: During a screening for novel and biotechnologically useful actinobacteria in insects, a novel actinomycete with antifungal activity, designated strain 1H-GS9(T), was isolated from the head of a Camponotus japonicus Mayr ant, which were collected from Northeast Agricultural University (Harbin, Heilongjiang, China). Strain 1H-GS9(T) was characterised using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of members of the genus Streptomyces. 16S rRNA gene sequence similarity studies showed that strain 1H-GS9(T) belongs to the genus Streptomyces with high sequence similarities to Streptomyces scopuliridis DSM 41917(T) (98.8 %) and Streptomyces mauvecolor JCM 5002(T) (98.6 %). However, phylogenetic analysis based on the 16S rRNA gene sequence indicated that it forms a monophyletic clade with Streptomyces kurssanovii JCM 4388(T) (98.6 %), Streptomyces xantholiticus JCM 4282(T) (98.6 %) and Streptomyces peucetius JCM 9920(T) (98.5 %). Thus, a combination of DNA-DNA hybridization experiments and phenotypic tests were carried out between strain 1H-GS9(T) and the above-mentioned five strains, which further clarified their relatedness and demonstrated that strain 1H-GS9(T) could be distinguished from these strains. Therefore, the strain is concluded to represent a novel species of the genus Streptomyces, for which the name Streptomyces formicae sp. nov. is proposed. The type strain is 1H-GS9(T) (=CGMCC 4.7277(T) = DSM 100524(T)).
    No preview · Article · Nov 2015 · Antonie van Leeuwenhoek
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    ABSTRACT: A novel actinomycete, designated strain 2C-HV3T, was isolated from the cuticle of Camponotus japonicus Mayr collected from Harbin, Heilongjiang province, north China and characterised using a polyphasic approach. The 16S rRNA gene sequence of strain 2C-HV3T showed that it has high sequence similarities with Microbispora bryophytorum NEAU-TX2-2T (99.9 %), Microbispora amethystogenes JCM 3021T (98.9 %) and Microbispora rosea subsp. rosea JCM 3006T (98.6 %). Phylogenetic analysis based on 16S rRNA and gyrB gene sequences demonstrated that strain 2C-HV3T clusters with M. bryophytorum NEAU-TX2-2T using two tree-making algorithms. Moreover, key morphological and chemotaxonomic properties also confirmed the affiliation of strain 2C-HV3T to the genus Microbispora. Longitudinal paired spores were observed to be born on short sporophores branching from the aerial hyphae. The cell wall was found to contain meso-diaminopimelic acid as the diagnostic diamino acid; madurose was found in the whole cell hydrolysate. The polar lipid profile was found to consist of diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside, ninhydrin-positive glycophospholipids, an unidentified phospholipid and an unidentified glycolipid. The predominant menaquinones were identified as MK-9(H2) and MK-9(H4). The major fatty acids were identified as 10-methyl C17:0 and iso-C16:0. However, a combination of DNA–DNA hybridization results and some phenotypic characteristics demonstrated that strain 2C-HV3T can be distinguished from its closely related relatives. Consequently, it is proposed that strain 2C-HV3T represents a new species of the genus Microbispora, for which the name Microbispora camponoti sp. nov. is proposed. The type strain is 2C-HV3T (=CGMCC 4.7281T = DSM 100527T).
    No preview · Article · Nov 2015 · Antonie van Leeuwenhoek
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    ABSTRACT: A novel actinomycete, designated strain NEAU-gq11(T), was isolated from wolfberry root (Lycium chinense Mill) and characterized using a polyphasic approach. Strain NEAU-gq11(T) was observed to form rough-surfaced spores that borne singly on the substrate hyphae but aerial mycelia were not developed. The organism showed closest 16S rRNA gene sequence similarity to Micromonospora saelicesensis Lupac 09(T) (99.4%), and phylogenetically clustered with M. chokoriensis 2-19/6(T) (99.3%), 'M. zeae' NEAU-gq9(T) (99.3%), M. violae NEAU-zh8(T) (99.1%), 'M. jinlongensis' NEAU-GRX11(T) (99.1%), M. zamorensis CR38(T) (99.0%), M. taraxaci NEAU-P5(T) (98.9%) and M. lupini Lupac 14N(T) (98.6%). Phylogenetic analysis based on the gyrB gene sequences also indicated that the isolate clustered with the above type strains except M. violae NEAU-zh8(T). The cell-wall peptidoglycan consisted of meso-diaminopimelic acid and glycine. The major menaquinones were MK-9(H8), MK-10(H6) and MK-10(H2). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acid was iso-C16:0. Furthermore, some physiological and biochemical properties and low DNA-DNA relatedness values enabled the strain to be differentiated from members of closely related species. Therefore, it is proposed that strain NEAU-gq11(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora lycii sp. nov. is proposed. The type strain is NEAU-gq11(T) (=CGMCC 4.7149(T)=DSM 46703(T)).The Journal of Antibiotics advance online publication, 28 October 2015; doi:10.1038/ja.2015.109.
    No preview · Article · Oct 2015 · The Journal of Antibiotics
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    ABSTRACT: Two novel actinobacteria, designated strains NEAU-JM1(T) and NEAU-CL2(T), were isolated from volcanic sediment and the rhizosphere soil of Paris polyphylla, respectively, collected from Jiling and Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The 16S rRNA gene sequence analysis showed that the two novel isolates exhibit 99.5 % 16S rRNA gene sequence similarity with each other. The phylogenetic analysis based on 16S rRNA gene sequence of strain NEAU-JM1(T) showed it to be closely related to Catellatospora methionotrophica JCM 7543(T) (99.4 % sequence similarity), Catellatospora coxensis DSM 44901(T) (99.4 %), Catellatospora citrea DSM 44097(T) (99.3 %) and Catellatospora chokoriensis JCM 12950(T) (99.2 %), and that of strain NEAU-CL2(T) to C. citrea DSM 44097(T) (99.4 %), C. methionotrophica JCM 7543(T) (99.3 %), C. chokoriensis JCM 12950(T) (99.3 %) and C. coxensis DSM 44901(T) (99.2 %). However, the DNA-DNA hybridization value between strains NEAU-JM1(T) and NEAU-CL2(T) was 62.2 %, and the values between the two strains and their close phylogenetic relatives were also below 70 %. With reference to phenotypic characteristics, phylogenetic data and DNA-DNA hybridization results, the two strains can be distinguished from each other and their close phylogenetic relatives. Thus, strains NEAU-JM1(T) and NEAU-CL2(T) can be concluded to represent two novel species of the genus Catellatospora, for which the names Catellatospora vulcania sp. nov. and Catellatospora paridis sp. nov. are proposed. The type strains are NEAU-JM1(T) (=CGMCC 4.7174(T) = JCM 30054(T)) and NEAU-CL2(T) (=CGMCC 4.7236(T) = DSM 100519(T)), respectively.
    No preview · Article · Oct 2015 · Antonie van Leeuwenhoek
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    ABSTRACT: A novel actinobacterium, designated strain NEAU-QY2T, was isolated from the leaves of Sonchus oleraceus L specimen, collected from Wuchang, Heilongjiang Province, China. A polyphasic study was carried out to establish the taxonomic positions of this strain. The organism formed single spores with rough surface on substrate mycelia. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NEAU-QY2T belonged to the genus Plantactinospora and formed a monophyletic clade with its closest related strains Plantactinospora endophytica YIM 68255T (99.2 %), Plantactinospora veratri NEAU-FHS4T (98.8 %) and Plantactinospora mayteni YIM 61359T (98.7 %), an association that was supported by a bootstrap value of 90% in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. However, the DNA-DNA hybridization relatedness values between strain NEAU-QY2T and the three strains were below 70 %. With reference to phenotypic characteristics, phylogenetic data and DNA-DNA hybridization results, strain NEAU-QY2T was distinguished from its most closely related strains and classified as a new species, for which the name Plantactinospora sonchi sp. nov. is proposed. The type strain is NEAU-QY2T (=CGMCC 4.7216T=JCM 30345T).
    No preview · Article · Oct 2015 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: A novel actinomycete, designated strain NEAU-A-2T, was isolated from the rhizosphere soil of Sansevieria trifasciata Prain collected from Heilongjiang province, northeast China. The taxonomic status of this organism was established using a polyphasic approach. The isolate formed irregular sporangia containing motile spores on the substrate mycelium. The whole-cell sugars were xylose and galactose. The predominant menaquinones were MK-9(H10), MK-9(H2), MK-10(H2) and MK-10(H4). The major fatty acids were iso-C15:0, iso-C16:0 and anteiso-C15:0. The polar lipids were diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, three unidentified phospholipids and an unidentified glycolipid. 16S rRNA gene sequence similarity studies showed that strain NEAU-A-2T belongs to the genus Actinoplanes with the highest sequence similarities to Actinoplanes globisporus NBRC 13912T (97.7 %), Actinoplanes ferrugineus NBRC 15555T (97.5 %), Actinoplanes toevensis MN07-A0368T (97.2 %) and Actinoplanes rishiriensis NBRC 108556T (97.2 %); similarities to other type species of this genus were lower than 97 %. Two tree-making algorithms showed that strain NEAU-A-2T formed a distinct clade with A. globisporus NBRC 13912T and A. rishiriensis NBRC 108556T. However, the low level of DNA-DNA relatedness allowed the isolate to be differentiated from the above-mentioned two Actinoplanes species. Moreover, strain NEAU-A-2T could also be distinguished from the most closely related species by morphological and physiological characteristics. Therefore, we concluded that the isolate NEAU-A-2T can be accommodated in the genus Actinoplanes named Actinoplanes rhizophilus sp. nov. The type strain is NEAU-A-2T (=CGMCC 4.7133T =DSM 46672T).
    No preview · Article · Sep 2015 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: A novel actinobacterial strain, designated strain NEAU-JM2(T), was isolated from volcanic sediment collected from Longwan, Jilin province, north China and characterized using a polyphasic approach. The strain was found to have morphological and chemotaxonomic characteristics typical of the members of the genus Micromonospora. Phylogenetic analysis of the16S rRNA gene sequence also indicated that strain NEAU-JM2(T) should be classified in the genus Micromonospora and showed that close relatives are Micromonospora maoerensis NEAU-MES19(T) (99.5 %) and Micromonospora matsumotoense JCM 9104(T) (98.8 %). However, phylogenetic analysis based on the gyrB gene sequence showed that the isolate forms a separate subclade away from the close relatives in the neighbour-joining tree and also recovered with the maximum-likelihood algorithm. The low level of DNA-DNA relatedness allowed the isolate to be differentiated from M. maoerensis NEAU-MES19(T) and M. matsumotoense JCM 9104(T). Furthermore, strain NEAU-JM2(T) could also be distinguished from its close phylogenetic relatives by cultural and physiological characteristics. Therefore, it is proposed that strain NEAU-JM2(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora vulcania sp. nov. is proposed. The type strain is NEAU-JM2(T) (=CGMCC 4.7144(T) = DSM 46711(T)).
    No preview · Article · Sep 2015 · Antonie van Leeuwenhoek
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    ABSTRACT: Two novel actinomycetes, designated strains NEAU-Jh1-3(T) and NEAU-Jh2-5(T), were isolated from muddy soil collected from a riverbank in Jiaohe, Jilin Province, north China. A polyphasic study was carried out to establish the taxonomic positions of these strains. The 16S rRNA gene sequence analysis showed that the two novel isolates exhibited 99.9 % 16S rRNA gene sequence similarity with each other and that they are closely related to Actinomadura viridis IFO 15238(T) (99.6, 99.6 %) and Actinomadura vinacea IFO 14688(T) (99.3, 99.3 %). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the two cultures clustered together and formed a cluster with A. viridis IFO 15238(T), A. vinacea IFO 14688(T) and Actinomadura rugatobispora IFO 14382(T). However, the DNA-DNA hybridization value between strains NEAU-Jh1-3(T) and NEAU-Jh2-5(T) was 63.6 %, and the values between the two strains and their close phylogenetic relatives were also below 70 %. With reference to phenotypic characteristics, phylogenetic data and DNA-DNA hybridization results, the two strains can be distinguished from each other and their close phylogenetic relatives. Thus, strains NEAU-Jh1-3(T) and NEAU-Jh2-5(T) represent two novel species of the genus Actinomadura, for which the names Actinomadura jiaoheensis sp. nov. and Actinomadura sporangiiformans sp. nov. are proposed. The type strains are NEAU-Jh1-3(T) (=CGMCC 4.7197(T) = JCM 30341(T)) and NEAU-Jh2-5(T) (=CGMCC 4.7211(T) = JCM 30342(T)), respectively.
    No preview · Article · Sep 2015 · Antonie van Leeuwenhoek
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    ABSTRACT: A novel actinomycete, designated strain NEAU-GQTH1-3T, was isolated from muddy soil collected from a stream in Qitaihe, Heilongjiang Province, northeast China and characterized using a polyphasic approach. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Sphaerisporangium and forms a stable clade with its closest relative Sphaerisporangium rubeum JCM 13067T(98.2 % 16S rRNA gene sequence similarity). Moreover, morphological and chemotaxonomic properties of strain NEAU-GQTH1-3T were also confirmed the affiliation of the isolate to the genus Sphaerisporangium. Cell wall contained meso-diaminopimelic acid and the whole-cell sugars were glucose, galactose, madurose, mannose and ribose. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, two phosphatidylinositol mannosides, phosphoglycolipid, an unidentified phospholipid and an unidentified glycolipid. The major menaquinones were MK-9(H4), MK-9(H6) and MK-9(H2). The predominant cellular fatty acids were iso-C16:0 and 10-methyl C17:0. Mycolic acids were absent. The DNA G+C content was 70.4 mol%. However, the low level of DNA-DNA relatedness and some phenotypic characteristics allowed the isolate to be differentiated from its closest relative. Therefore, it is proposed that strain NEAU-GQTH1-3T represents a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium aureirubrum sp. nov. is proposed. The type strain is NEAU-GQTH1-3T (=CGMCC 4.7199T=JCM 30346T).
    No preview · Article · Aug 2015 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: A mesophilic endophytic filamentous bacterium, designated strain NEAU-gxj18T, was isolated from soybean root [Glycine max (L.) Merr.] collected from Harbin, Heilongjiang Province, China and characterized using a polyphasic approach. Growth was observed at 20-40 °C, optimal temperature for growth was 37 °C. Aerial mycelium was absent on all the media tested. Substrate mycelia were well-developed and formed abundant single endospores with smooth surface. The only menaquinone was MK-7. The diagnostic diamino acid was meso-diaminopimelic acid. The whole-cell sugars were ribose, glucose and galactose. The major fatty acids were iso-C15:0, C13:0 and iso-C17:0. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol. The DNA G+C content was 49.9 mol %. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain NEAU-gxj18T was phylogenetically related to members of the family Thermoactinomycetaceae with the highest sequence similarity to Geothermomicrobium terrae YIM 77562T (93.35 %). On the basis of the morphological and chemotaxonomic characteristics, phylogenetic analysis and characteristic patterns of 16S rRNA gene signature nucleotides, strain NEAU-gxj18T represents a novel species of a new genus within the family Thermoactinomycetaceae, for which the name Baia soyae gen. nov., sp. nov. is proposed. The type strain of Baia soyae is strain NEAU-gxj18T (=CGMCC 4.7223T =DSM 46831T).
    No preview · Article · Jul 2015 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: A Gram-stain positive, non-motile, mesophilic actinomycete, designated strain NEAU-YJC4(T) was isolated from the root of a marigold (Tagetes erecta L.) collected in Heilongjiang Province, northeast China, and characterized using a polyphasic approach. Morphological and chemotaxonomic properties of strain NEAU-YJC4(T) were consistent with the description of the genus Catellatospora. 16S rRNA gene sequence similarity studies showed that strain NEAU-YJC4(T) belongs to the genus Catellatospora, being most closely related to Catellatospora bangladeshensis JCM 12949(T) (98.7 %). Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain NEAU-YJC4(T) formed a monophyletic clade with the closest relative. A combination of DNA-DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-YJC4(T) can be distinguished from C. bangladeshensis JCM 12949(T). Therefore, it is proposed that strain NEAU-YJC4(T) represents a novel species of the genus Catellatospora, for which the name Catellatospora tagetis sp. nov. is proposed. The type strain is NEAU-YJC4(T) (=CGMCC 4.7176(T) = JCM 30053(T)).
    No preview · Article · Jun 2015 · Antonie van Leeuwenhoek
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    ABSTRACT: A novel actinomycete, designated strain NEAU-Jh3-20T, was isolated from the river sediment collected from South river in Jilin Province, north China and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-Jh3-20T should be assigned to the genus Streptomyces and forms a distinct branch with its closest neighbour Streptomyces vitaminophilus DSM 41686T(97.09 %). Moreover, key morphological and chemotaxonomic properties were also confirmed the affiliation of strain NEAU-Jh3-20T to the genus Streptomyces. Cell wall contained LL-diaminopimelic acid and the whole-cell hydrolysates were glucose and ribose. The phospholipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylinositol mannosides (PIMs) and an unidentified phospholipid (PL). The predominant menaquinones were MK-9(H8) and MK-9(H6). Major fatty acids were C16:0, C18:0, anteiso-C15:0 and anteiso-C17:0. The DNA G+C content was 72.2 mol%. A combination of DNA-DNA hybridization result and some phenotypic characteristics demonstrated that strain NEAU-Jh3-20T could be distinguished from its closest relative. Therefore, it is proposed that strain NEAU-Jh3-20T represents a novel species of the genus Streptomyces, for which the name Streptomyces tyrosinilyticus sp. nov. is proposed. The type strain is NEAU-Jh3-20T (=CGMCC 4.7201T=DSM 42170T).
    No preview · Article · Jun 2015 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: A novel actinomycete, designated strain NEAU-Jh2-17T, was isolated from the muddy soil collected from a riverbank in Jilin Province, north China and characterized using a polyphasic approach. The 16S rRNA gene sequence of strain NEAU-Jh2-17T showed highest similarities to those of Streptomonospora nanhaiensis 12A09T (99.26 %), Nocardiopsis rosea YIM 90094T (97.31 %), Streptomonospora halophila YIM 91355T (97.24 %) and Streptomonospora arabica DSM 45083T (97.02 %). Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain NEAU-Jh2-17T located in a cluster consisted of type species of the genus Streptomonospora and formed a stable clade with S. nanhaiensis 12A09T in two tree-making algorithms. Furthermore, key morphological and chemotaxonomic properties were also confirmed the affiliation of strain NEAU-Jh2-17T to the genus Streptomonospora. Cell wall contained meso-diaminopimelic acid as diagnostic diamino acid and the whole-cell hydrolysates contained glucose, ribose and galactose. The polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidylinositol mannoside (PIM), two unknown phospholipids (PLs) and two unknown glycolipids (GLs). The predominant menaquinones were MK-10(H2), MK-10(H8), MK-10(H6) and MK-10(H4). Major fatty acids were C18:0 10-methyl, anteiso-C17:0, C16:0 10-methyl, iso-C15:0, C17:0 10-methyl and C18:0. The DNA G+C content was 71.82 mol%. However, a combination of DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-Jh2-17T could be distinguished from its closely related relatives. Therefore, it is proposed that strain NEAU-Jh2-17T represents a novel species of the genus Streptomonospora, for which the name Streptomonospora halotolerans sp. nov. is proposed. The type strain is NEAU-Jh2-17T (=CGMCC 4.7218T=JCM 30347T).
    No preview · Article · Jun 2015 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: A novel endophytic actinomycete, strain NEAU-TX1-15(T), was isolated from moss, collected from Wuchang, Heilongjiang province, north China. A polyphasic taxonomic study was carried out to establish the status of strain NEAU-TX1-15(T). Morphological and chemotaxonomic properties of strain NEAU-TX1-15(T) are consistent with the description of the genus Actinoallomurus. Strain NEAU-TX1-15(T) was observed to form short spiral or looped spore chains on aerial hyphae. The cell wall peptidoglycan was found to contain lysine and meso-diaminopimelic acid. The major menaquinones were identified as MK-9(H6) and MK-9(H8). The only phospholipid identified was phosphatidylglycerol. The major fatty acid was identified as iso-C16:0. Analysis of the 16S rRNA gene sequence supports the assignment of the novel strain to the genus Actinoallomurus, as it exhibits 99.2 % gene sequence similarity to that of Actinoallomurus yoronensis NBRC 103686(T). However, the low level of DNA-DNA relatedness allowed the strain to be differentiated from its close relative. Moreover, strain NEAU-TX1-15(T) could also be differentiated from A. yoronensis NBRC 103686(T) and other Actinoallomurus species showing high 16S rRNA gene sequence similarity (>98.0 %) by cultural and physiological characteristics. Therefore, the combination of phenotypic and chemotaxonomic data, and the DNA-DNA hybridization value, indicated that strain NEAU-TX1-15(T) represents a novel species of the genus Actinoallomurus for which the name Actinoallomurus bryophytorum sp. nov. is proposed. The type strain is NEAU-TX1-15(T) (=CGMCC 4.7200(T) = JCM 30340(T)).
    No preview · Article · Jun 2015 · Antonie van Leeuwenhoek
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    ABSTRACT: A novel actinobacterium, designated strain NEAU-TPG4T was isolated from rhizosphere soil of Pinus koraiensis Sieb. et Zucc. collected from Luobei, Heilongjiang Province, north China, and characterized using a polyphasic approach. Morphological and chemotaxonomic properties of strain NEAU-TPG4T were consistent with the description of the genus Dactylosporangium. The strain formed finger-shaped sporangia on short sporangiophores that emerged directly from substrate hyphae. The cell-wall peptidoglycan consisted of meso- and 3-hydroxy-diaminopimelic acids; arabinose, xylose and glucose were found as whole-cell sugars. The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones were identified as MK-9(H8) and MK-9(H6). The predominant cellular fatty acids were identified as iso-C16:0, anteiso-C15:0, anteiso-C17:0 and C18:0. Phylogenetic analysis using 16S rRNA gene sequences also indicated that the strain should be classified in the genus Dactylosporangium and showed that the closest relatives were Dactylosporangium maewongense Japan Collection of Microorganism (JCM) 15933T (99.6 %), Dactylosporangium siamense NBRC 106093T (99.6 %), Dactylosporangium aurantiacum JCM 3083T (99.5 %) and Dactylosporangium luteum JCM 17685T (99.5 %). However, the low level of DNA–DNA relatedness and several cultural and physiological characteristics allowed the strain to be distinguished from its closest relatives. Thus, it is proposed that strain NEAU-TPG4T represents a novel Dactylosporangium species. Dactylosporangium cerinum sp. nov. The type strain of D. cerinum is NEAU-TPG4T (=CGMCC 4.7152T = DSM 46712T).
    No preview · Article · May 2015 · Antonie van Leeuwenhoek
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    ABSTRACT: Two Gram-stain positive, aerobic actinomycete strains, designated NEAU-YHS12 and NEAU-YHS15(T), were isolated from the root of Corydalis yanhusuo L. collected from Wuchang, Heilongjiang Province, northeast China. Phylogenetic analysis of 16S rRNA gene sequences showed that the two strains are closely related to one another (99.8 % similarity), and had the closest relationship with Sphaerisporangium cinnabarinum JCM 3291(T) (98.7, 98.6 %), Sphaerisporangium flaviroseum YIM 48771(T) (98.6, 98.6 %), Sphaerisporangium melleum JCM 13064(T) (98.5, 98.4 %) and Sphaerisporangium dianthi NEAU-CY18(T) (98.4, 98.4 %). DNA-DNA hybridization value between strains NEAU-YHS12 and NEAU-YHS15(T) was 82 ± 1.4 %, and the values between the two strains and the closely related type strains were well below 70 %. The two strains also shared a number of phenotypic characteristics that were distinct from the closely related species. Both strains were observed to contain MK-9(H6), MK-9(H4) and MK-9(H2) as the detected menaquinones. The cell wall peptidoglycan was found to contain meso-diaminopimelic acid. The phospholipid profiles were found to contain diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, phosphoglycolipid, and an unidentified phospholipid. The major fatty acids were identified as iso-C16:0, C17:1 ω7c, C18:0 and iso-C15:0. On the basis of the genetic and phenotypic properties, it is proposed that strains NEAU-YHS15(T) and NEAU-YHS12 be classified as representatives of a novel species of the genus Sphaerisporangium, for which the name Sphaerisporangium corydalis sp. nov is proposed. The type strain is NEAU-YHS15(T) (CGMCC 4.7148(T) = DSM46732(T)).
    No preview · Article · May 2015 · Antonie van Leeuwenhoek