[Show abstract][Hide abstract] ABSTRACT: Objective
To study the incidence and potential causes of monozygotic twins after in vitro fertilization and embryo transfer (IVF-ET).
A retrospective study was performed on women carrying monozygotic twins (MZTs) after conventional IVF-ET treatment at the Third Affiliated Hospital of Guangzhou Medical College in China from January 2003 to May 2009. The incidence and the miscarriage rate for MZTs following IVF-ET were examined in relation to maternal age, duration of infertility, type and dose of hormone treatment, conventional IVF-ET cycles versus intracytoplasmic sperm injection (ICSI) cycles, the use of fresh or frozen-thawed embryos, and day (post-fertilization) of embryo transfer.
Sixteen MZT pregnancies occurred in 2 161 patients (incidence of 0.74%), of which 5 miscarried (31.25%). No significant difference was found between MZT and non-MZT groups in terms of maternal age, duration of infertility, duration of gonadotropin (Gn) administration, dosage of Gn, number of oocytes retrieved, number of oocytes fertilized, or number of embryos transferred (P>0.05). The incidence of MZT was not statistically different between conventional IVF-ET cycles and ICSI cycles, between fresh embryos transfer cycles and frozen-thawed embryo cycles, or between different transfer days (P>0.05).
The incidence of MZTs following IVF-ET treatment greatly exceeds that observed following spontaneous conception. Intracytoplasmic sperm injection, frozen-thawed procedures, and embryo transfer on different days were not correlated with an increased incidence of MZT pregnancies.
No preview · Article · Jun 2012 · Journal of Reproduction and Contraception
[Show abstract][Hide abstract] ABSTRACT: Objective
To investigate the viability and maturation of frozen-thawed human immature oocytes exposed to different temperature of vitrification and warming solutions.
The immature oocytes in germinal vesicle (GV) and matephase I (Mi) stages were collected from our ICSI patients and exposed to different temperature of vitrification and warming solutions before frozen in the freezing/thawing procedures. The different temperature groups were as follows: Group A, equilibration solution at 37 °C, vitrification solution at room temperature, warming solution at 37 °C; Group B, both vitrification and warming solution at room temperature; Group C, both vitrification and warming solutions at 37 °C; Group D, the frozen-thawed oocytes and the fresh oocytes were cultured for in vitro maturation. The survival rate and maturation rate were compared among groups. The oocytes were examined using immunofluorescent stainingand confocal microscopy to check the spindle configuration and chromosome arrangement.
The survival rates and Mn rates of GV stage oocytes in groups A, B, C were 100%(15/15), 81.3%(13/16), 68.8%(11/16) and 33.3%(2/6), 83.3%(10/12),72.7% (8/11), respectively. The survival rate of group C was significantly lower than that of the control (P<0.05). The normal spindle and chromosome configuration were only observed in group B, with the rates of 20% (2/20) and 10% (1/10), respectively. The survival rates of Mi stage in groups A, B, C were 71.4%(10/14), 100% (12/12) and 83.3%(10/12), no significantly difference from that of the control (100%, 14/14). The Mn rates of Mi stage in groups A, B and C were 0%(0/14), 66. 7%(8/12) and 80% (8/10), respectively. The M II rate in group A was significantly lower than that in other groups (P<0.01). Only one oocyte in group C was found with normal spindle and chromosome configurations.
The appropriate operation temperature of vitrification and warming solutions can improve the outcomes of the vitrified-thawed human immature oocytes.
No preview · Article · Jun 2011 · Journal of Reproduction and Contraception
[Show abstract][Hide abstract] ABSTRACT: To study the effect of patient age, the number and quality of embryo transferred on pregnancy outcome of in vitro fertilization-embryo transfer (IVF-ETs).
A retrospective study was performed in infertile women who underwent a total of 1800 cycles of IVF-ET and intracytoplasmic sperm injection (ICSI). The patients were divided into three age groups, namely<30 years group, 30-35 years group, and>or=35 years group. The clinical pregnancy rate and the multiple pregnancy rate were compared when 1, 2 or 3 embryos and 0, 1, 2 or 3 good-quality embryos were transferred.
In patients<30 years, no significant differences was found in the clinical pregnancy rate between 1, 2 and 3 embryos transfer groups; 2 and 3 good-quality embryos transfer resulted in similar pregnancy rate, which was significantly higher than that resulted from 0 and 1 good-quality embryo transfer. Multiple pregnancy was not found in 1 embryo transfer group. In patients aged 30-35 years, the pregnancy rate showed no significant differences not only between 1 and 2 embryos transfer groups, but also between 2 and 3 good-quality embryos transfer groups; multiple embryo transfer led to significantly increased multiple pregnancy rate. In patients aged>or=35 years, the transfer of 1, 2 and 3 embryos resulted in similar pregnancy rate; transfer of 3 good-quality embryos had obviously higher pregnancy rate than 0, 1 and 2 good-quality embryos transfer groups. Increased numbers of embryos and good-quality embryos transferred were both associated with increased multiple pregnancy rate.
One good-quality embryo transfer in patients<30 years and 2 good-quality embryos transfer in patients>or=30 yeas can obtain ideal pregnancy rate and reduce the incidence of multiple pregnancy. For patients aged>or=35 years, transfer of only good-quality embryo is recommended.
No preview · Article · Aug 2010 · Nan fang yi ke da xue xue bao = Journal of Southern Medical University
[Show abstract][Hide abstract] ABSTRACT: To compare the development of abnormal pronuclear zygotes after intracytoplasmic sperm injection (ICSI) and analyze their genetic polymorphism.
Four hundred and ninety three abnormal pronuclear zygotes after ICSI were divided into three groups based on the number of pronuclei: 347 nonpronuclear oocytes, 71 monopronuclear zygotes and 75 multipronuclear zygotes. All of them were cultured in the medium of Vitrolife G5 series(TM). Sixteen short tandem repeats (STR) of seven blastocysts were then analyzed by ABI3100.
The cleavage rate of nonpronuclear group (25.4%) was lower than that of the others (P<0.01), the proportion of blocked embryos in nonpronuclear group (48.9%) was significantly higher than that of the others (P<0.05), but the blastocyst rate showed no significant difference in three groups (P>0.05). The genetic polymorphism of the 16 STRs showed that the blastocysts from the nonpronuclear and multipronuclear were diploid, and one of the blastocysts from nonpronuclear oocyte was Y-bearing.
The zygotes with abnormal pronuclei after ICSI might have development potential, and the blastocysts from nonpronuclear oocytes and multipronuclear zygotes could be diploid.
No preview · Article · Aug 2010 · Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics
[Show abstract][Hide abstract] ABSTRACT: To investigate the in vitro maturation of human oocytes (IVM) from pregnant late term, natural cycles and Gn stimulating cycles and the effect of granulose cells on IVM from pregnant late term.
A total of 1086 immature oocytes were obtained including 633 oocyte-cumulus complexes (OCCs) and 453 denuded oocytes (DOs). OCCs were divided into pregnant late term group, natural cycle group and IVM group, and DOs were divided into pregnant late term group, natural cycle group and controlled ovarian hyperstimulation (COH) group. All the oocytes were matured in IVM culture system and fertilized by ICSI. The embyos were cultured to blastcyst stage except that those in IVM group were transferred into the uterus. The main outcomes were assessed including maturation rate (MR), fertilization rate (FR), cleaverage rate (CR), and blastulation rate (BR) (natural cycle group, pregnant late term group and COH group) and pregnancy rate per transfer cycle (PR) of IVM group.
MR of OCCs in pregnant late term group, natural cycle group and COH group was 74.3%, 76.9% and 82.2%, respectively, showing statistical difference between pregnant late term cycle group and IVM group. No statistical difference was observed in FR, CR or BR between the three groups. For IVM cycle group, clinical pregnancy rate of 20% per aspiration was achieved. For DOs, MR of COH group (86.0%) was significantly higher than that of the natural cycle group (72.5%) and pregnant late term group (72.7%) (P<0.01). FR, CR and BR showed no statistical difference among the 3 groups. No difference was found in MR, FR, CR and BR between OCCs group and DOs group from pregnant late term. CONLUSIONS: The oocytes from pregnant late term have the same development potential as those from natural cycles or Gn stimulating cycles in vitro, and provide a new source of donor oocytes. Granulose cells do not affect the IVM from pregnant late term.
No preview · Article · Jul 2009 · Nan fang yi ke da xue xue bao = Journal of Southern Medical University