Y Kobayashi

St. Marianna University School of Medicine, Kawasaki Si, Kanagawa, Japan

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Publications (5)4.68 Total impact

  • K. Fukamoto · K. Shirai · S. Sato · R. Kanekatsu · K. Kiguchi · Y. Kobayashi
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    ABSTRACT: When an excised section of larval integument is implanted into a larva, the growth rate of the epidermal cells of the implanted tissue increases to form a cyst in vivo. In order to clarify the processes associated with in vivo cyst formation in the sweet potato hornworm, Agrius convolvuli, we investigated the progression thereof using light- and electron microscopy. Immunohistochemical techniques using eCBP (epidermal carotenoid binding protein) as a specific marker for detecting larval epidermal cells, and BrdU-incorporation for examining DNA synthesis in the epidermal cells were also undertaken. The results indicated that in vivo cyst formation proceeded through the following five phases: (1) hemocytes aggregate around the periphery of the transplanted integument, (2) forming a thick mass of aggregated cells. This is followed by (3) DNA synthesis, not only in the cells near the periphery, but also in the entire epidermal cell layer. Then, (4) the epidermal cells at the leading edge penetrate the cell mass until continuity of the integument is complete, (5) finally they secrete new cuticle inside the cyst. Given the simplicity, high reproducibility and ease with which these experimental procedures can be conducted, the system of in vivo cyst formation in insect is a useful model for studying the more detailed mechanisms associated with wound recovery, such as epidermal elongation at the site of integument damage.
    No preview · Article · Oct 2006 · Journal of Insect Biotechnology and Sericology
  • Y Kobayashi · K Kiguchi · B Ishizuka

    No preview · Article · Feb 2006 · International Journal of Gynecology & Obstetrics
  • Y Fukada · A Amemiya · K Kohno · R Sunami · Y Kobayashi · K Hoshi
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    ABSTRACT: This study was designed to correlate fetuses in a normal prenatal population who exhibited transient nuchal translucency (NT) with both prenatal course and pregnancy outcome. The fetuses with abnormal NT were followed by ultrasonography at 1-2-week intervals during their prenatal course. Fetuses with NT who exhibited no detectable congenital malformations were defined as the NTO group; fetuses with abnormal NT and structural malformations but no chromosomal abnormalities were placed in the SM group; and fetuses with abnormal NT, structural malformations, and chromosomal abnormalities were categorized as the CA group. The groups were compared by: maternal age, weeks of gestation by ultrasound and the NT value at the initial exam, the maximum NT value, and the duration of abnormal NT. During the study period, 92 fetuses with abnormal NT were found, monochorionic twins excluded. Of the 92 fetuses, 80 were in the NTO group, 10 were in the SM group, and two were in the CA group. The maternal age and the gestational weeks at the initial diagnosis were not significantly different in the NTO and the SM groups. However, the NT value at the initial diagnosis, maximum NT value, and the duration of abnormal NT were significantly greater in the SM group than those values in the NTO group. Fetuses with transient nuchal translucency commonly had structural malformations, particularly fetuses with significant and large persistent NT.
    No preview · Article · Jan 2003 · International Journal of Gynecology & Obstetrics
  • B Ishizuka · Y Kuribayashi · Y Kobayashi · N Hamada · Y Abe · A Amemiya · T Aoki · T Satoh
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    ABSTRACT: To compare the intensity of stress responses caused by laparoscopy combined with CO2 insufflation (CI) with those caused by mechanical elevation of the abdominal wall (MEA). Prospective study (Canadian Task Force classification I). Tertiary care university hospital. Thirty-one women. Intervention. Laparoscopy, 16 with CI and 15 with MEA. Circulating levels of norepinephrine, epinephrine, cortisol, and interleukin (IL)-6 were compared in the two groups. Arterial partial pressure of CO2 (PaCO2) did not increase during laparoscopy by either method. Plasma norepinephrine and epinephrine levels increased after extubation in the MEA group but not in the CI group. Circulating cortisol levels in both groups increased during laparoscopy; mean intraoperative levels were higher in the CI group. Serum IL-6 levels increased after extubation in the MEA but not the CI group. Mean heart rate increased during laparoscopy in both groups, whereas blood pressure increased only in the CI group. More patients in the MEA group required postoperative analgesia. Laparoscopy with MEA caused more pronounced adrenosympathetic and cytokine responses than that with CI when PaCO2 was maintained within the range of normocapnia.
    No preview · Article · Sep 2000 · The Journal of the American Association of Gynecologic Laparoscopists

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