Yukihiro Hasegawa's scientific contributions

Publications (11)

Publications citing this author (293)

    • In addition, SOX7 protein expression was regulated by miR-9. TGF-b1 has been implicated in the pathogenesis of lung cancers [30][31][32]. Interestingly, in the present study, we found the expression of miR-9 and SOX7 was up-regulated by TGF-b1 treatment in NSCLC cells, despite the observation that SOX7 was a direct target of miR-9.
    [Show abstract] [Hide abstract] ABSTRACT: MicroRNA (miR)-9 plays different roles in different cancer types. Here, we investigated the role of miR-9 in non-small-cell lung cancer (NSCLC) cell invasion and adhesion in vitro and explored whether miR-9 was involved in transforming growth factor-beta 1 (TGF-β1)-induced NSCLC cell invasion and adhesion by targeting SOX7. The expression of miR-9 and SOX7 in human NSCLC tissues and cell lines was examined by reverse transcription-quantitative polymerase chain reaction. Gain-of-function and loss-of-function experiments were performed on A549 and HCC827 cells to investigate the effect of miR-9 and SOX7 on NSCLC cell invasion and adhesion in the presence or absence of TGF-β1. Transwell-Matrigel assay and cell adhesion assay were used to examine cell invasion and adhesion abilities. Luciferase reporter assay was performed to determine whether SOX7 was a direct target of miR-9. We found miR-9 was up-regulated and SOX7 was down-regulated in human NSCLC tissues and cell lines. Moreover, SOX7 expression was negatively correlated with miR-9 expression. miR-9 knockdown or SOX7 overexpression could suppress TGF-β1-induced NSCLC cell invasion and adhesion. miR-9 directly targets the 3' untranslated region of SOX7, and SOX7 protein expression was down-regulated by miR-9. TGF-β1 induced miR-9 expression in NSCLC cells. miR-9 up-regulation led to enhanced NSCLC cell invasion and adhesion; however, these effects could be attenuated by SOX7 overexpression. We concluded that miR-9 expression was negatively correlated with SOX7 expression in human NSCLC. miR-9 was up-regulated by TGF-β1 and contributed to TGF-β1-induced NSCLC cell invasion and adhesion by directly targeting SOX7.
    Full-text · Article · Mar 2017
    • In fact, microscopic haematuria and renal function deterioration commenced during erlotinib therapy and no case of CrGN was reported for the other anticancer agents used in this case. Interstitial lymphoplasmacytic infiltration might be associated with erlotinib, because two cases of tubulointerstitial nephritis with gefitinib have been reported [6,7]. In summary, we describe a case of pauci-immune CrGN and acute renal failure, possibly induced by erlotinib.
    Full-text · Article · Nov 2009
    • To inhibit angiogenesis is key in controlling invasion and metastasis of cancer cells and this inhibition could improve the prognosis and survival rates for patients. Studies [15,16] have confirmed that VEGF has a high expression in non-small cell and small cell lung cancers. TianGang Xie et al [17] confirmed a high level of expression of VEGF in peripheral blood for lung cancer patients using ELISA methods.
    [Show abstract] [Hide abstract] ABSTRACT: In our research,we study the effect of 131iodine-labeled histamine-indomethacin (131I-His-IN). We focus on its in vivo therapeutic effect and anti-tumor mechanisms in Lewis-bearing lung cancer. 131I-His-IN was administered by garage to the mice. At different timepoints, we made autoradiography (ARG) slices to observe the distribution of 131I-His-IN in the cellular, and the sliced samples underwent hematoxylin and eosin (HE) staining for observation of tumor necrosis. Before treatment, the groups of mice underwent 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography-computed tomography (PET-CT) scans ,and they were then given physiologic saline, iodine 131 (131I), indomethacin (IN), Histamine-indomethacin (His-IN), and 131I-His-IN, respectively, three times daily for seven days. Seven days later, all the mice underwent 18F-FDG PET-CT scans again. We calculated the maximum standard uptake value (SUVmax) of the region of interest (ROI) and tumor inhibition rate at the same time. In ARG groups, black silver particle was concentrated in the nucleus and cytoplasm. 131I-His-IN mainly concentrated in tumor tissues. At 8 hours after 131I-His-IN, the radioactivity uptake in tumor tissue was higher than in other organs (F=3.46,P<0.05). For the 18F-FDG PET-CT imaging, the tumor tissus’es SUVmax of the ROI was lower compared to other groups after the treatment with 131I-His-IN. The tumor inhibitory rate (54.8%) in 131I-His-IN group was higher than in other groups, too. In the 131I-His-IN group the vascular endothelial growth factor (VEGF) decreased gradually compared to other groups. The tumor tissue necrotized obviously in 131I-His-IN group. Through these animal experiments, we found 131I-His-IN could inhibit the Lewis lung cancer cells. 131I-His-IN focused at the cell nucleus and cytoplasm. It could reduce VEGF and increase tumor inhibitory rate. At the same time, 18F-FDG PET-CT scan could be used for a curative effect and monitoring of disease prognosis.
    Full-text · Article · Mar 2013
    • It was concluded that paclitaxel and nedaplatin doses could not be escalated due to severe pulmonary toxicity at level 1. Another phase I/II trial of weekly paclitaxel (35 mg/m 2 ) and nedaplatin (20 mg/m 2 ) for 6 weeks revealed that this regimen is safe and effective for NSCLC with concurrent TRT [30]. A phase II study led by Oshita et al. [31] evaluated a dose of nedaplatin at 50 mg/m 2 , and irinotecan at 60 mg/m 2 on days 1 and 8 every 4 weeks for 2–4 cycles with concurrent TRT (2 Gy per day, totaling 60 Gy).
    [Show abstract] [Hide abstract] ABSTRACT: Background Concurrent chemotherapy and radiation is the standard treatment for unresectable stage III Lung adenocarcinoma. However, no optimal concurrent chemotherapeutic regimen has been described. This study aimed to assess concurrent pemetrexed, nedaplatin and thoracic intensity-modulated radiotherapy followed by consolidation pemetrexed/nedaplatin for unresectable Stage IIIA/B lung adenocarcinoma. Methods Patients with unresectable stage III lung adenocarcinoma received thoracic intensity-modulated radiotherapy at 60–64 Gy in 30–32 fractions, concurrently with two cycles of 500 mg/m2 pemetrexed, with nedaplatin doses escalating from 60 mg/m2 (level 1) to 70 mg/m2 (level 2) and 80 mg/m2 (level 3). Consolidation consisted of three pemetrexed/nedaplatin (500 mg/m2, 60 mg/m2) cycles every 3 weeks after concurrent therapy. The primary objective of the safety was to determine the maximum-tolerated dose (MTD). The secondary endpoints included response rate, PFS and OS. ResultsFifteen patients were enrolled, including 3, 6 and 6 individuals in the first, second, and third dose levels, respectively. Three cases of dose-limiting toxicities (grade 3 hepatitis, pneumonitis, and grade 4 thrombocytopenia), including one and two patients at levels 2 and 3, respectively, were observed and resulted in discontinued/delayed treatment. Response rates were 86.7 % (95 % confidence interval [CI], 64.2–97.8 %) and 64.3 % (95 % CI, 38.3–85.4 %) at chemoradiation and treatment completions, respectively. Median OS was 30.0 months (95 % CI, 16.4–43.6 months); 2-year OS was 44.0 % (95 % CI, 18.7–69.2 %). Median PFS was 12.0 months (95 % CI, 6.9–17.0 months), and the 2-year PFS 27.0 % (95 % CI, 4.7–49.3 %). Conclusions Full dose 500 mg/m2 of pemetrexed and nedaplatin 70 mg/m2 could be used safely with thoracic intensity-modulated radiotherapy for inoperable stage III lung adenocarcinoma. Further evaluation of stage III lung adenocarcinoma management is warranted. Trial registrationThis study was retrospectively registered at Chinese Clinical Trial Registry (ChiCTR-OPN-16008316, April 2016).
    Full-text · Article · Oct 2016
    • In the earlier published studies, several non-bacteriological diagnostic parameters have been evaluated for the early laboratory diagnosis of TPE. These include (a) histopathology in pleural biopsy (Diacon et al., 2002), (b) nucleic acid amplification assay (Dinnes et al., 2007; Trajman et al., 2007), (c) estimation of biomarkers such as adenosine deaminase (Chen et al., 2004; Trajman et al., 2007), (d) antibody to mycobacterial glycolipids (Morimoto et al., 2006), (e) lipoarabinomannan (Yokoyama et al., 2005) and (f) IFN-γ (Greco et al., 2003; Jiang et al., 2007). These above diagnostic approaches do hold promise, but the sensitivities and specificities reported in these studies are highly variable and hence restricted the routine application as a diagnostic tool in patients with TPE.
    Article · Oct 2012
    • Eotaxin is the strongest and the most specific factor for eosinophils, acting through interactions with CCR3 receptors [33]. The eosinophil counts in an infiltrated organ are proportional to the eotaxin concentrations at the inflammatory site34353637, with poor asthma control and increased symptom severity. In addition, we also used the chemoattractants PAF and RANTES to evaluate the eosinophil activity in all groups of individuals.
    [Show abstract] [Hide abstract] ABSTRACT: Background A clear relationship between asthma and obesity has been reported, but the mechanisms remain unclear. The aim of this study was to evaluate the influence of obesity on eosinophil activity (chemotaxis and adhesion) in asthmatic children and adolescents compared with cells from healthy volunteers. Methods Asthmatic obese (AO), asthmatic non-obese (ANO), non-asthmatic obese (NAO) and non-asthmatic non-obese (NANO) individuals were included in the present study. The chemotaxis of eosinophils after stimulation with eotaxin (300 ng/ml), platelet-activating factor (10 μM; PAF) and RANTES (100 ng/ml) was performed using a microchemotaxis chamber. The eosinophil peroxidase activity was measured to determine the adhesion activity of eosinophils cultivated on fibronectin-coated plates. The serum leptin, adiponectin, TNF-α and IgE levels were quantified using ELISA assays. Results The serum IgE levels and eosinophil counts were significantly higher in asthmatic (obese and non-obese) individuals compared with non-asthmatic individuals (obese and non-obese). Spontaneous eosinophil chemotaxis was greater in the AO group compared with either the ANO or NANO groups. The activation of eosinophils using eotaxin and PAF increased eosinophil chemotaxis in the AO group. RANTES treatment increased eosinophil chemotaxis in the NAO group compared with the NANO or ANO groups. The activation of eosinophils using eotaxin significantly increased eosinophil adhesion in the AO group compared with other groups. The serum leptin and TNF-α levels were higher in obese subjects (asthmatic and non-asthmatic), whereas the levels of adiponectin did not significantly differ among these groups. Conclusion This study is the first to show increased eosinophilic activity (chemotaxis and adhesion) associated with high serum leptin and TNF-α levels in atopic asthmatic obese children and adolescents compared with non-obese healthy volunteers.
    Full-text · Article · Jun 2013
    • For this purpose, great attention has been paid to the cancerous portion of the lung nodule in determining a classification or one or more histological sub-type [5]. More recently, lung nodule histological composition is being described and evaluated qualitatively, extending beyond the cancerous component of the nodule to include vascular, fibrotic, and necrotic tissue [6, 7]. These studies have indicated there is a statistically significant correlation between the proportions of necrosis and fibrosis within a lung nodule and patient outcome.
    [Show abstract] [Hide abstract] ABSTRACT: Gaining a complete and comprehensive understanding of lung cancer nodule histological compositions and how these tissues are represented in radiological data is important not only for expanding the current knowledge base of cancer growth and development but also has potential implications for classification standards, radiological diagnosis methods and for the evaluation of treatment response. In this study we generate large scale histological segmentations of the cancerous and non-cancerous tissues within resected lung nodules. We have implemented a processing pipeline which allows for the direct correlation between histological data and spatially corresponding computed tomography data. Utilizing these correlated datasets we evaluated the statistical separation between Hounsfield Unit (HU) histogram values for each tissue type. The findings of this study revealed that lung cancer nodules contain a complex intermixing of cellular tissue types and that trends exist in the relationship between these tissue types. It was found that the mean Hounsfield Unit values for isolated lung cancer nodules imaged with computed tomography, had statistically significantly different values for non-solid bronchoalveolar carcinoma, solid cancerous tumor, blood, and inactive fibrotic stromal tissue.
    Full-text · Article · Mar 2011