X.-Y. Wang

Peking University People's Hospital, Peping, Beijing, China

Are you X.-Y. Wang?

Claim your profile

Publications (10)4.28 Total impact

  • J.-Y. Chen · X.-Y. Wang · Y. Pan · J. Ye · M. Zhang · Q.-M. Sun · S.-H. Ma
    [Show abstract] [Hide abstract]
    ABSTRACT: Objective: To analyze the genetic characters of VP1 gene of ECHO virus 6 (E6) KM57-09 strain isolated from cerebrospinal fluid of children with aseptic meningitis in Kunming City, China in 2009. Methods: E6 KM57-09 strain was isolated from cerebrospinal fluid of children with aseptic meningitis by using RD and Hep-2 cells, of which VP1 gene was amplified by RT-PCR and sequenced. The sequencing result was blasted by using NCBI BLAST software. The nucleotide and deduced amino acid sequences of the VP1 gene were edited, blasted and spliced by using Omigan software, of which the results were analyzed by using Mega4.1 software and compared with those of 18 reference strains. Results: The isolate was identified as E6, of which the length of nucleotides at VP1 region, 867 bp, was identical to those of other E6 isolates. The homologies of nucleotide and amino acid sequences of KM57-09 strain were 77.6%-96.0% and 95.2%-99.0% to those of patial ECHO virus strains reported in GenBank, 96.0% and 99.0% to those of 2010D0010005 strain isolated in Shandong Province, while were 79.7%-80.9% and 95.8%-97.2% to those of other isolates in China, respectively. Phylogenetic tree revealed that KM57-09 strain was located in the same branch as that of 2010D0010005 strain, while in different branches from those of other isolates. Conclusion: KM57-09 isolate was identified as E6 belonging to one of three distinct clusters in China.
    No preview · Article · May 2012 · Chinese Journal of Biologicals
  • D.-P. Pu · X.-Y. Wang · J. Zhang · Y.-Q. Zhu · Y.-Q. Hu · X.-R. Zhou
    [Show abstract] [Hide abstract]
    ABSTRACT: Objective: To examine the therapeutic effect of C II TA inhibition in collagen-induced arthritis (CIA), using a delivery system tailored to target C II TA gene by small interfering RNA (siRNA). Methods: Mice with collagen-induced arthritis were injected intravenously with C II TA siRNA. The clinical score was monitored for up to 4 weeks after treatment. The severity of inflammation of mouse joint was evaluated by histological examination. Real-time PCR was used to determine the cytokine mRNA expression. Cytokine production was measured by ELISA from serum. T cell proliferation was examined by MTT method. Results: IFN-γ and IL-17 were elevated in CIA mice, but were inhibited significantly by C II TA siRNA either prevention or intervention of autoimmune arthritis. Collagen specific T cell proliferation was significantly suppressed. Increased level of IL-4 by T cells was observed in C II TA siRNA treated group compared with that of control group. Conclusion: Our findings indicate that systemic RNAi-mediated C II TA gene silencing is effective in the treatment of CIA and regulateds the balance of Th1/Th2 differentiation.
    No preview · Article · Apr 2012
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: To study the prevalence and types of androgenetic alopecia (AGA) in China and to compare the results with those reported in Caucasians and other Asian people. Methods: A community-based study was carried out in six cities of China (each city selected from one province). Subjects received a face-to-face interview and answered the questionnaires. The degree of AGA was classified according to the Hamilton and Ludwig classifications. Results: Totally 17 886 subjects were interviewed and 15 257 completed the questionnaires (the response rate 85.3%). The overall prevalence of AGA was 12.8%. In males, the prevalence of AGA was 21.3%. Grade IV was the most common type. In females, the prevalence of AGA was 6.0%, which was significantly lower than that of males (P < 0.01). Ludwig grade I was the most common type. The prevalence of AGA varied between cities. Family history was present in 26.9% of people with AGA. Conclusion: The overall prevalence of AGA in Chinese was 12.8%, which is much lower than that in Caucasians. The prevalence of AGA in males is 3.5 times larger than that in females.
    No preview · Article · Dec 2010
  • X.-L. Ding · Q.-X. Xu · Q.-M. Sun · J.-L. Qian · X.-Y. Wang · H.-Y. Huang · J. Du
    [Show abstract] [Hide abstract]
    ABSTRACT: Objectives: To evaluate the therapeutic efficacy and safety of 304 nm UVB in the targeted treatment of vitiligo. Methods: Eighty-seven patients with vitiligo were treated by 304 nm UVB twice a week, with Dualight™ UV 120-2 targeted phototherapy system (Theralight Inc. USA). The skin type and the minimal erythema dose (MED) were measured before treatment. The initial dosage was 2 times of MED, and the dosage was gradually increased. The efficacy and safety were evaluated 3 months later. Results: Out of 87 cases, 14 cured, 15 significantly improved, 53 moderately improved, and 5 without improvement. The total response rate was 33.3%. The adverse reactions were slight erythema and pruritus which resolved spontaneously. The efficacy was related to the clinical stage of the lesions, not to the location of the lesions. Conclusion: 304 nm UVB therapy is an effective and safe method to treat vitiligo, which could be used to protect the normal skin around the lesions.
    No preview · Article · Nov 2010
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: There are racial differences in the prevalence and types of androgenetic alopecia (AGA). The prevalence of AGA has been studied in Caucasians and in some Asian people. In China, although there have been some epidemiological studies carried out in single cities or regions, no multicentre population-based study has been reported. To study the prevalence and types of AGA in China and to compare the results with those previously reported in Caucasians and in other Asian people. A community-based study was carried out in six cities of China. Subjects were interviewed face-to-face and completed questionnaires. The degree of AGA was classified according to the Norwood and Ludwig classifications. In total 17 886 subjects were interviewed and 15 257 completed the questionnaires. In men, the overall prevalence of AGA was 21.3%, with 2.8% in men aged 18-29 years, 13.3% in those aged 30-39 years, 21.4% in those aged 40-49 years, 31.9% in those aged 50-59 years, 36.2% in those aged 60-69 years and 41.4% in those aged 70 years and over. The most common type was frontal and vertex hair loss. A small number of subjects (3.7%) showed 'female pattern' hair loss. In women, the prevalence of AGA was 6.0%, with 1.3% in women aged 18-29 years, 2.3% in those aged 30-39 years, 5.4% in those aged 40-49 years, 7.5% in those aged 50-59 years, 10.3% in those aged 60-69 years and 11.8% in those aged 70 years and over. Ludwig grade I was the most common type. The prevalence of AGA varied between cities. A positive family history was present in 29.7% of men and 19.2% of women with AGA. The prevalence of AGA in Chinese men and women was lower than in Caucasians and similar to that in Koreans.
    Preview · Article · Apr 2010 · British Journal of Dermatology
  • X. Hua · Y.-H. Bian · X.-L. Sun · Y.-H. Ji · J. Zhang · X.-Y. Wang · X.-R. Zhou
    [Show abstract] [Hide abstract]
    ABSTRACT: Objective: To investigate the effects of trichostatin A (TSA) on the mice model of collagen induced arthritis(CIA). Methods: Mice model of rheumatoid arthritis(RA) was induced in DBA/1 mice with type II collagen. Paws were scored for histological severity of arthritis. The severity of inflammation of mouse joint was evaluated by histological examination. Real-time PCR was used to determine the cytokine mRNA expression. Cytokine production was measured by ELISA from serum, spleen cell culture or dendritic cell and T cell co-culture supernatant. T cell proliferation was examined by MTT method. Results: TSA can significantly suppress the severity of the arthritis in CIA. IFN-γ was elevated in CIA mice, but was inhibited significantly by TSA introduced either at the same time with immunization or at the onset of manifestation of arthritis. Collagen specific T cell proliferation was significantly suppressed by introduction of TSA. Increased level of IL-4 by T cells was observed in TSA treated group compared to that of control group. Conclusion: IL-4 level was increased and played a critical role in the protective effects of TSA in CIA. TSA suppresses the progress of CIA by regulates the balance of Th1/Th2 differentiation.
    No preview · Article · Jan 2010
  • X.-L. Ding · G.-X. Zhou · X.-R. Zhou · X.-Y. Wang
    [Show abstract] [Hide abstract]
    ABSTRACT: AIM: To investigate the effects and the mechanism of triptolide (TL) on proliferation and apoptosis of pancreatic cancer in vitro. METHODS: Pancreatic tumor cell lines PANC-1, ASPC-1 and SW1990 were treated with different concentrations of TL for 24 h and then, cell death was determined by Typan Blue Staining. Annexin V/PI double staining was performed to evaluate TL-induced apoptosis using flow cytometry. The expression of 5-lipoxygenase (5-LOX) and concentration of its downstream product LTB4 were determined by real time PCR, Western blot and ELISA. 5-LOX cDNA stable transfected SW1990 cells were established successfully. After treatment with TL, it was examined for overexpression of 5-LOX on TL-induced apoptosis. RESULTS: TL induced prominent growth inhibition and apoptosis in human pancreatic cancer cell lines. After treatment at 50 μg/L, the cell viability was 70.5% ± 6.8%, 61.2% ± 5.6% and 52.8% ± 5.3% of PANC-1, ASPC-1 and SW1990, respectively, which were significantly decreased compared with control group (P < 0.05). The apoptosis at 12 h evaluated by AnnexinV positive cells increased in TL-treated group compared with control group (24.2 ± 3.23 vs 9.5 ± 2.18, P < 0.05). TL significantly down-regulated 5-LOX expression in these cell lines and decreased LTB4 concentration in supernatant (P < 0.01, compared with control group). Furthermore, overexpression of 5-LOX in SW1990 cells made them more resistant to TL induced apoptosis, significantly inhibited the TL mediated cell death and apoptosis (P < 0.01 or 0.05, compared with control group). CONCLUSION: Inhibition of 5-LOX pathway of arachidonic acid metabolism is associated with TL's anti-proliferation and pro-apoptotic activity. It also provides evidence that TL has clinic therapeutic value for patients with pancreatic cancer.
    No preview · Article · Dec 2008 · World Chinese Journal of Digestology
  • Q. Wu · X.-Y. Wang · X.-R. Zhou · W. Tang · L. Su
    [Show abstract] [Hide abstract]
    ABSTRACT: AIM: To investigate the influence of dendritic cells (DCs) loaded with HBsAg from peripheral blood monocytes of patients with chronic hepatitis B (CHB) on the differentiation of autologous T helper 1 (Th1). METHODS: Mononuclear cells were isolated from CHB patients using the methods of density gradient centrifugation (Ficoll-Hypaque) and adherence. The adherent monocytes were incubated with recombinant human GM-CSF and interleukin-4 (IL-4) to induce DC generation. The phenotypes on DCs including CD80, CD86, CD40 and HLA-DR were determined by flow cytometry (FCM). DCs' ability to stimulate allogenic mixed lymphocyte reaction (AMLR) was detected by CCK-8 assay. The concentration of IL-12 in DC supernate was tested by enzyme linked immunosorbent assay (ELISA). CD4+ Th cell subpopulation in peripheral blood of CHB patients was isolated and purified by immunomagnetic beads. Subsequently, purified CD4 + Th cells were co-cultured with autologous mature DCs. The differentiation of Th1/Th2 was detected by FCM using intracellular staining of interferon-γ (IFN-γ) and IL-4. ELISA assay was also used to quantify the IFN-γ and IL-4 in co-culture supernate. RESULTS: In comparison with those in the control group, the expression levels of CD80, CD86, CD40 and HLA-DR and the concentration of IL-12 in DC supernate of the IFN-γ group, HBsAg group and HBsAg + IFN-γ group were higher, and the capability of DCs to stimulate allogenic lymphocytes to proliferate in the three groups was also stronger. Meanwhile, the percentages of Th1 cells in CD4+ T cells and the level of IFN-γ in co-culture supernate of the IFN-γ group, HBsAg group and HBsAg + IFN-γ group were higher (Th1 cells: 10.76% ± 3.98%, 11.43% ± 4.32%, 15.28% ± 4.73% vs 7.84% ± 3.10%, P < 0.01; IFN-γ: 578 ± 47 mg/L, 496 ± 92 mg/L, 784 ± 97 mg/L vs 342 ± 34 mg/L, P < 0.05). However, the percentages of Th2 cells in CD4+ T cells and the level of IL-4 in the three groups were lower (Th2 cells: 1.43% ± 0.96%, 1.68% ± 0.16%, 0.92% ± 0.21% vs 2.61% ±1.27%, P < 0.01; IL-4:187 ± 52 mg/L, 169 ± 38 mg/L, 89 ± 37 mg/L vs 226 ± 48 mg/L, P < 0.05). The most significant effect was observed in the HBsAg + IFN-γ group. CONCLUSION: DCs loaded with HBsAg can improve the insufficient differentiation of Th1 cells resulted from dysfunction of DCs in vivo in CHB patients.
    No preview · Article · Apr 2008 · World Chinese Journal of Digestology
  • Y. Han · X.-Y. Wang · Y.-H. Ji · A.-G. Shen · X.-L. Sun · Y.-Q. Hu · X.-R. Zhou · Q. Wu
    [Show abstract] [Hide abstract]
    ABSTRACT: Objective: To study the expression and localization of β-1,4- galactosyltransferase I (β-1,4-GalT I) in CD4+ helper T(T H)lymphocytes and the effect of β-1,4-GalT I on adhesion ability of CD4+ TH cells. Methods: CD4+ TH cells were isolated by CD4 positive immunomagnetic beads. The expression and localization of β-1,4-GalT I in CD4+ TH cells was detected by RT-PCR, immunofluorescence and laser scanning confocal microscopy. Activation and differentiation of CD4+ TH cells were introduced by cytokines such as rhIL-2, rhIL-12 and rhIL-4. The expression level of β-1,4-GalT I in CD4+ TH cells was measured by semi-quantitative RT-PCR and flow cytometry. The adhesion ability of CD4 + TH cells was compared by cell adhesion assay. α-lactalbumin was used to interfere the activity and substrate specificity of cell surface β-1,4-GalT I, then the influence of that on the adhesion ability of CD4+ TH cells was analyzed by cell adhesion assay. Results: Long and short β-1,4-GalT I were expressed and localized on the cytomembrane and cytoplasm of CD4+ TH cells. The expression level of long β-1,4-GalT I and cell surface β-1,4-GalT I, and the adhesion ability of CD4+ TH cells activated with rhIL-2 increased as compared with unactivated CD4+ TH cells. The expression level of long β-1,4-GalT I and cell surface β-1,4-GalT I were both positively correlated with the adhesion ability of CD4+ TH cells. The adhesion ability of CD4+ TH cells decreased after the incubation with α-lactalbumin. Conclusion: β-1,4-GalT I expression in CD4+ TH cells is closely correlated with cell adhesion ability, which suggests that β-1,4-GalT I may play an important role in the adhesion process of CD4 + TH cells.
    No preview · Article · Oct 2007
  • X.L. Ding · T.L. Wang · Y.W. Shen · X.Y. Wang · C. Zhou · S. Tian

    No preview · Article ·