W. Li

Harbin Medical University, Charbin, Heilongjiang Sheng, China

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Publications (9)3.88 Total impact

  • W. Li · J.F. Fei · Q. Yang · B.L. Li · C. Lin · Q. Yue · Q.G. Meng
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    ABSTRACT: The objective of this study was to observe the acute cytotoxic effects of hematoporphyrin monomethyl ether sonodynamic therapy (HMME-SDT) on hypertrophic scar fibroblasts of rabbit ears. We first assessed the effects of different irradiation times and HMME concentrations on the survival of hypertrophic scar fibroblasts using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to determine the optimum irradiation time and HMME concentration. The hypertrophic scar fibroblast cell suspensions of the rabbit ears were divided into four groups, the survival rates were detected using the MTT assay, and the type of cell death was detected by Annexin V/propidium iodide (PI) double staining flow cytometry. Our results showed that HMME-SDT significantly reduced the viability of hypertrophic scar fibroblasts of rabbit ears at ultrasonic irradiation times of 30, 60, and 90 s, but not 10 s (P < 0.05). HMME alone had no significant effect on the cell survival rate at any irradiation time (P > 0.05). In contrast, the cell survival rate was significantly decreased at an irradiation time of 10 s and HMME concentrations of 20 and 50 μg/mL (P < 0.05). Furthermore, Annexin V/PI double staining showed necrosis and apoptosis of the hypertrophic scar fibroblasts. Given our results, HMME might be an effective sound-sensitive agent for SDT as it has a significant lethal effect on hypertrophic scar fibroblasts of rabbit ear cultured in vitro. HMME-SDT may therefore provide a new method for the treatment of hypertrophic scar formation.
    No preview · Article · May 2015 · Genetics and molecular research: GMR
  • W. Li · Q.-G. Meng · Z.-G. Bi
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    ABSTRACT: Aim: The sural nerve flap failure is still a problem for the patient with reconstructive surgery. The cause were attributed to infection, tension and insufficient debridement. Negative pressure when applied to soft tissue defects results in a significant increase ingranulation tissue production compared to similar conventionally treated wounds in the patients. There were no clinical signs of infection and cultures were negtive in any of the wounds that achieved complete closure. Methods: We used vacuum sealing drainage (VSD) combined with sural nerve flap for the reconstruction of defects of lower one-third of the leg, foot and ankel.with exposed tendon or bone in 25 patients between 2007 and 2009-The defects were covered with no major complications. Results: All 25 flaps survived after the operation. The blood supply and venous return of the skin flaps were good. In 2 flaps, there was a small amount of distal marginal necrosis, which was excised and closed spontaneously or skin grafted. Slight venous congestion of the flap occurred in one patients, which were cured by removing some sutures. No other clinical abnormalities were discovered. Mean follow-up was 17 months (5-24 months). The flap was warm, and no venous congestion was observed. Conclusion: We concluded that the vacuum sealing drainage combined with sural nerve flap can be recommended for the treatment of acute traumatic soft tissue defects and for soft tissue defects complicated by exposed bone and/or implants.
    No preview · Article · Jun 2012 · Minerva Ortopedica e Traumatologica
  • W Li · W J Yue · Q Yue · D W Yang · Q G Meng
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    ABSTRACT: The therapeutic value of the transforming growth factor beta 1 (TGF-b) in transplantation has been reported; However, cell-mediated gene therapy using TGF-b is not applied to the organ transplantation widely. This study was to evaluate whether TGF-b-modified donor spleen cell specific transfusion in rat heterotopic allo-limb transplantation could induce tolerance tolerogenicity and prolong allograft's survival time. The Splenic T-cell in Wistar rats responsing to donor spleen cells which received TGF-b-transduced were severely impaired.The Survival time of Sprague-Dawley Allograft-limb in Wistar rats given TGF-b-modified donor spleen cells (5¥106 cells/well, administration of donor TGF-b-transduced donor spleen cells 7 days before transplantation) was extended modestly but significantly.
    No preview · Article · Dec 2010 · Panminerva medica
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    ABSTRACT: Aim. The aim of this paper was to study the effect of nicotine on herniated or degenerative lumbar disc tissue and its induced peripheral blood white cells to produce inflammatory mediators such as No and EL-6. Methods. Ten specimens of lumbar discs were obtained from patients with protrusion of lumbar disc when operated on. The peripheral blood samples were drawn from ten healthy adult volunteers. Monocytes were separated from each sample. Then, cell culture was performed as the following combinations: Group 1 was disc tissue and medium. Group 2 was disc tissue + nicotine + medium. Group 1 was deactivated disc tissue + monocytes + medium. Group 4 was deactivated disc tissue + monocytes + nicotine + medium. Group 5 was monocytes + medium. Group 6 was monocytes+nicotine + medium. The samples were incubated in CO2 culture box for 72 hours, then taken out, the medium was collected for assayed the quantity of NO and IL-6 (using enzyme-linked immunosorbent assays, ELISA). Results. The quantity of NO and IL-6 secreted by Group 2 was significantly greater than Group 1 (P<0.05, P<0.01).The quantity of NO and IL-6 secreted by Group 4 was significantly greater than Group 3 (P<0.01, P<0.01). The quantity of NO and EL-6 secreted by Group 6 was significantly greater than Group 5 (P<0.05, P<0.05). The quantity of NO and IL-6 secreted by Group 3 was significantly greater than Group 1 and Group 5 (P<0.05). Conclusion. Herniated or degenerative disc tissue was of the capacity to secrete inflammatory mediators such as NO and IL-6, and it was increased significantly after nicotine stimulation. Disc tissue could induce monocytes to produce high concentration NO and EL-6 and it was increased significantly after nicotine stimulation.
    No preview · Article · Feb 2010 · Minerva Ortopedica e Traumatologica
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    W Li · Y-Y Lian · W-J Yue · Q Yang · Q Yue · Q-G Meng · C-B Zhao
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    ABSTRACT: This study investigated the effects of non-steroidal anti-inflammatory drugs (NSAIDs) on peri-operative blood loss during elective total hip replacement. Patients were randomized to receive enteric-coated diclofenac 50 mg (n = 18), rofecoxib 12.5 mg (n = 17) or placebo (n = 16) administered orally three times daily for 2 weeks prior to surgery. Severe adverse effects resulting in discontinuation of trial participation occurred in six patients in the diclofenac group, five patients in the rofecoxib group and two patients in the placebo group; all drop-outs occurred at various times after surgery. Compared with placebo, peri-operative blood loss increased by 32% in the diclofenac group and by 7% in the rofecoxib group. Total mean +/- SD blood loss was 1040 +/- 136 ml in the diclofenac group, 844 +/- 83 ml in the rofecoxib group and 789 +/- 82 ml in the placebo group. Thus, administering a non-selective NSAID 2 weeks prior to elective total hip replacement significantly increases peri-operative blood loss.
    Preview · Article · Mar 2009 · The Journal of international medical research
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    ABSTRACT: Aim. The aim of this study was to revision hip arthroplasties in ankylosing spondylitis. Methods. The authors performed 21 revision hip arthroplasties in 16 patients with ankylosing spondylitis using S-ROM modular femoral stem. The mean age of the patients at index operation was 46.3 years (range 31-61) and the mean follow-up was 53.5 months (range 48-64). At the final follow-up, all patients were able to ambulate without any walking aids. No S-ROM femoral component was revised. Results. The mean Harris hip score improved from 57.2 (range 35-74) preoperatively to 90.2 points (range 73-100) postoperatively and the outcome was classified as good or excellent in 17 hips (81 %). Fixation of the femoral component was classified as stable with bone ingrowth in 15 hips (71.4%), stable with fibrous in-growth in 2 hips (9.5%), and radiolucent loosening in 4 hips (19.1%). Five hips developed a pedestal at the tip of femoral component Femoral osteolysis was found in 3 hips (14.2%): 3 hips in Gruen zones 1 and 7, one hip in zone 7, and two hip in zone 1. One hip underwent acetabular revision because of breakage of polyethylene liner and the well-fixed femoral component was left in situ. Thigh pain developed in one patient (6.2%). Kaplan-Meier survival was 81% at 64 months, with radiographic loosening as an end-point when two hips were at risk. Conclusion. Satisfactory results of midterm clinical and radiographic follow-up can be achieved using S-ROM modular femoral stem for revision of femoral stem in ankylosing spondylitis.
    No preview · Article · Feb 2009 · Minerva Ortopedica e Traumatologica
  • H.-J. Zhang · W. Li · Q. Yang · Q.-G. Meng
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    ABSTRACT: Background: Intrathymic injection of spleen cells is commonly used to inhibit or reduce rejection in heart transplantation. However, there are few reports regarding its application in nerve transplantation. Objective: To investigate the influence of intrathymic injection spleen cells in induction of sciatic allograft immunotolerance in rats. Design, time and setting: Randomized controlled animal experiment was performed at the Animal Experiment Center of Harbin Medical University between June 2007 and June 2008. Materials: Thirty male SD rats were selected as recipients, and randomly divided into autograft, allograft and allogenic antigen groups (n=10). In addition, 20 male Wistar rats were selected as donors. Methods: The biceps femoris and semitendinosus muscles were separated and the sciatic nerve was exposed. About 1cm sciatic nerve was cut off under the infrapiriformis foremen. Wistar rat nerve was bridged to nerve defect of SD recipient rat. Nerve autografting was performed in autograft group, and allogenic antigen was injected into allogenic antigen group following nerve allografting. Main outcome measures: Two weeks after the surgery, nerve electrophysiology, pathology, interleukin-2 (IL-2), mixed lymphocyte culture and delayed type hypersensitivity were detected. Results: Motor nerve conduction velocity in allogenic antigen group was no significantly different from autograft group (P> 0.05), but was significantly better than allogrft group (P < 0.05). The detections in pathology and electric microscope were consistent with results of motor nerve conduction velocity. The mixed lymphocyte culture and IL-2 level in allogenic antigen group was significantly greater than allograft group (P < 0.05). Conclusion: Intrathymic injection of allogenic antigen induces specific sciatic allograft immunotolerance of rats.
    No preview · Article · Jan 2009 · Journal of Clinical Rehabilitative Tissue Engineering Research
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    ABSTRACT: Background: Transforming growth factor beta 1 (TGF-β1) has been used for peripheral nerve transplantation to inhibit or reduce rejection, but under some conditions, TGF has a positive regulatory effect. Thus, it is necessary to study dose-effect relationship in order to obtain reliable data evidences. Objective: To investigate the dose-effect correlation of immune rejection with local injection of different doses TGF-β1 plasmid. Design, time and setting: The randomized control animal experiment was performed at the Animal Experimental Center of Harbin Medical University from June 2007 to April 2008. Materials: Twenty male Wistar rats were used as donors. Fifty clean male Sprague Dawley rats as recipients were randomly assigned into 5 groups, 10 in each group, comprising nerve autograft group, nerve allograft group, low-dose, middle-dose and high-dose TGF-β1 plasmid local injection groups. pAdTrack-CMV-TGF-β1 plasmid, pAdEasy-1-Bj51833 cells were presented by Professor Zeng from Laboratory of Communicable Disease, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology. Methods: A 1-cm section of the sciatic nerve was cut off under the infrapiriformis foramen. Donor nerve bridge was connected with nerve defect region. (10, 20, 40 μ g) TGF-β1 plasmid was injected into rats in the low-dose, middle-dose and high-dose TGF-β1 plasmid local injection groups. Blank plasmid was injected into rats in the nerve allograft group. Main outcome measures: Six weeks after surgery, lab-tests were examined, nerve electrophysiology, pathology, mixed lymphocyte culture and axon counting. Results: Motor nerve conduction velocity and axon number in high-dose TGF-β1 plasmid local injection group was close to nerve autograft group (P > 0.05), and better than low-dose TGF-β 1 plasmid local injection group (P < 0.05). Pathology and transmission electron microscope demonstrated that outcome of transplanted nerve segment in the high-dose TGF-β1 plasmid local injection group was close to that in the nerve autograft group (P > 0.05), and better than low-dose TGF-β1 plasmid local injection group. The mixed lymphocyte culture and delayed type hypersensitivity in the high-dose TGF-β1 plasmid local injection group were better than in the low-dose TGF-β 1 plasmid local injection group (P < 0.05). Conclusion: Local injection of TGF-β1 plasmid could reduce immune rejection after sciatic nerve allograft transplantation in rats. The reduction of immune rejection changed synchronously with local injection of different doses TGF-β1 plasmid (10-40 μ g).
    No preview · Article · Sep 2008 · Journal of Clinical Rehabilitative Tissue Engineering Research
  • F.R. Li · J.X. Yang · B.L. Li · Z.T. Zhang · W. Li

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