Yukihide Iwamoto

Kyushu University, Hukuoka, Fukuoka, Japan

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Publications (811)1947.32 Total impact

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    ABSTRACT: Aims: Angiofibroma of soft tissue (AFST) is a rare soft tissue neoplasm characterized by a fibroblastic cytomorphology and a prominent vascular structure. AFSTs possess a novel fusion gene NCOA2-AHRR/AHRR-NCOA2 or GTF2I-NCOA2, enabling a useful approach to diagnosing AFST. Morphologically, AFSTs cross a wide spectrum, making diagnosis a challenge. Thus we decided to review AFST cases and to report previously unknown histological features, which we confirmed by genetic analysis METHODS AND RESULTS: We reviewed 276 cases diagnosed as solitary fibrous tumors/hemangiopericytomas (232 cases), unclassified tumors of fibroblastic differentiation (36 cases), and recently diagnosed AFSTs (8 cases), and retrieved 13 cases compatible with AFST. Immunohistochemical staining was performed for these cases, all 13 of which were analyzed by RT-PCR and FISH methods. The histological findings were as follows: amianthoid fibers, extravasation of red blood cells, hemosiderin deposition, aggregates of foamy histiocytes, cystic change, necrosis, and hemorrhage. Immunohistochemically, the tumor cells were positive for EMA (4/13 cases), desmin (6/13 cases), CD163 (13/13 cases), CD68 (7/13 cases), estrogen receptor (13/13 cases), progesterone receptor (3/13 cases), and STAT6 (1/13 cases, weak nuclear staining); but they were negative for CD34, alpha-smooth muscle actin, muscle-specific actin, S-100 protein, pan-cytokeratin, MDM2, and CDK4. AHRR-NCOA2 fusion gene was detected in 8 cases, and NCOA2 gene rearrangement in 9 cases. Conclusion: We revealed the previously unreported histological variation and immunohistochemical findings of AFST and confirmed them by genetic methods. The results suggested that AFST should be considered in the diagnosis of fibrous or fibrohistiocytic tumors with the above histological features. This article is protected by copyright. All rights reserved.
    No preview · Article · Feb 2016 · Histopathology
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    ABSTRACT: Background: Poor femoral implant fit to resected bone surfaces recently has been the motivation for several new total knee arthroplasty implant designs. Implant overhang risks adverse soft-tissue interaction while uncovered cut bone surfaces (underhang) risks increased postoperative bleeding or development of heterotopic bone. Methods: Femoral implant fit was studied systematically, and without the influence of surgical variation, by virtually implanting standard and narrow width femoral components (Bi-Surface 5) using preoperative computed tomography data for 150 varus osteoarthritic knees in Japanese patients. Overhang and underhang rates and bone widths were determined by gender. Results: Narrow femoral components helped avoid or minimize overhang in most female and some male knees. Although anterior width in the narrow components closely matched female bone width, the femoral component was necessarily displaced laterally to avoid overhang in the anteromedial portion. Consequently, there was significant medial underhang in the distal and posterior zones. Conclusions: Ideally, the anterior femoral flange should be shifted 2 to 2.5mm laterally relative to the distal and posterior aspects to provide optimal femoral bone coverage in this prosthesis. The current study also confirmed that this modification can be generalized to the other two currently available "narrow type" prostheses. This geometric modification might allow surgeons to select a femoral component with slightly wider mediolateral dimensions in the distal and posterior aspects to minimize underhang, while eliminating anterior overhang. Level of evidence: III.
    No preview · Article · Feb 2016 · The Knee
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    ABSTRACT: Exposure to dioxin-related compounds results in many adverse health effects. Several studies have examined the effects of dioxin-related compounds on human bone metabolism with inconsistent results. In Japan in 1968, accidental human exposure to rice oil contaminated with dioxin-related compounds led to the development of Yusho oil disease. The aim of this study was to determine whether exposure to dioxin-related compounds was associated with bone mineral density in Yusho patients. In 2010, 262 women and 227 men underwent dual-energy X-ray absorptiometry bone scans as part of the nationwide Yusho health examination. Serum levels of polychlorinated dibenzo-p-dioxin, polychlorinated dibenzofurans, and non-ortho polychlorinated biphenyls were measured using high-resolution gas chromatography and high-resolution mass spectrometry. When adjusted for prefecture, 1,2,3,4,7,8-HxCDD and 2,3,7,8-TCDF were significantly positively associated with Z-scores in men. No congeners were positively associated with Z-scores in women. After adjustment for prefecture and body mass index, one congener, 1,2,3,4,6,7,8-HpCDD, was negatively associated with Z-scores in women. In contrast, no congeners remained significant in men after adjusting for body mass index. This may suggest that 1,2,3,4,6,7,8-HpCDD has a negative effect on bone mineral density in women; however, the findings should be interpreted carefully, because no increase in the serum level of this congener was observed in patients with Yusho disease.
    No preview · Article · Feb 2016 · Chemosphere
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    ABSTRACT: N-myc downstream regulated gene 1 (NDRG1) is a responsible gene for a hereditary motor and sensory neuropathy-Lom (Charcot-Marie-Tooth disease type 4D). This is the first study aiming to assess the contribution of NDRG1 to differentiation of macrophage lineage cells, which has important implications for bone remodeling and inflammatory angiogenesis. Ndrg1 knockout (KO) mice exhibited abnormal curvature of the spine, high trabecular bone mass, and reduced number of osteoclasts. We observed that serum levels of macrophage colony-stimulating factor (M-CSF) and macrophage-related cytokines were markedly decreased in KO mice. Differentiation of bone marrow (BM) cells into osteoclasts, M1/M2-type macrophages and dendritic cells was all impaired. Furthermore, KO mice also showed reduced tumor growth and angiogenesis by cancer cells, accompanied by decreased infiltration of tumor-associated macrophages. The transfer of BM-derived macrophages from KO mice into BM-eradicated wild type (WT) mice induced much less tumor angiogenesis than observed in WT mice. Angiogenesis in corneas in response to inflammatory stimuli was also suppressed with decreased infiltration of macrophages. Taken together, these results indicate that NDRG1 deficiency attenuates the differentiation of macrophage lineage cells, suppressing bone remodeling and inflammatory angiogenesis. This study strongly suggests the crucial role of NDRG1 in differentiation process for macrophages.
    Preview · Article · Jan 2016 · Scientific Reports
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    ABSTRACT: Background: In contrast to the acetabular cup version (CV), stem version (SV) measurement on the crosstable lateral radiograph has not been validated. We, therefore, investigated: (1) the difference of SV on the crosstable lateral radiograph from that measured using computed tomography data and (2) the reliability of SV measurement on the crosstable lateral radiograph. Methods: We examined the correlations of the crosstable-CV and crosstable-SV with true-CV (operative cup anteversion) and true-SV (neck version relative to the posterior condylar line) obtained using computed tomography data from 103 patients. We also produced the digital reconstructed crosstable lateral radiograph with adjusted hip flexion and rotation and defined SV on this image as the true-crosstable SV. Results: Significant positive correlations were noted between the crosstable-CV and true-CV, with a correlation coefficient of 0.79. In contrast, the correlation between the crosstable-SV and true-SV was very low (r = 0.30), with significantly higher deviation from the true-SV values when compared with the crosstable-CV (standard deviation, 11.6° vs 6.5°, P < .001). The true-crosstable SV was correlated with true-SV (r = 0.81); however, the average difference was 23.5° ± 8.9°. Conclusion: Crosstable-SV differed from true-SV in the measurement plane and did not correlate well with the true-SV, suggesting the unreliability of its measurements. Therefore, the crosstable-SV is not recommended for clinical use.
    No preview · Article · Jan 2016 · The Journal of arthroplasty
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    ABSTRACT: We histopathologically examined a surgically resected full specimen obtained from an early-stage spontaneous osteonecrosis of the knee (SPONK). On a mid-coronal cut section of the resected medial femoral condyle, a linear fracture line paralleling the subchondral bone endplate was found. Histopathologically, prominent callus formation was seen comprising of reactive woven bone and granulation tissue on both sides of the fracture. Fracture-related bone debris was focally observed at the osteochondral side of the fracture. Definitive features of antecedent bone infarction such as creeping substitution and bone marrow necrosis were not detected. These findings suggested that SPONK was the result of a subchondral fracture rather than primary osteonecrosis.
    No preview · Article · Jan 2016 · Skeletal Radiology
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    ABSTRACT: The secondary use of medical data to improve medical care is gaining much attention. We have analyzed electronic clinical pathways for improving the medical process. The analysis of clinical pathways so far has used statistics analysis models, however as issue remains that the order, and multistory spatial and time relations of the each factor could not be analyzed. We constructed an Outcome tree system that shows the greatest significant relation for each factor. The Hip replacement arthroplasty clinical pathway was analyzed by the system, and the outcome variance of the clinical pathway was visualized. The results indicate the path of patient's who have a long hospitalization stay and extracted four critical indicators.
    Full-text · Article · Dec 2015 · Procedia Computer Science
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    ABSTRACT: Cam deformity could lead to suboptimal articulation by causing secondary femoroacetabular impingement after periacetabular osteotomy; however, the inherent femoral head-neck morphology in dysplastic hips and the effect of an additional osteoarthritic deformity have not been well described. We compared femoral head-neck morphology using three-dimensional imaging of normal and dysplastic hips in pre/early (Tönnis grade 0 and 1) and advanced stage osteoarthritis (Tönnis grade 2). Using computed tomography, we measured the circumferential α-angle and head-neck offset ratio in 68 dysplastic hips and 24 normal hips. Locations of the head-neck junction were represented by the clock position. In the pre/early group, the α-angle was significantly larger at the anterosuperior and inferior aspects (1, 2, and 5-7 o'clock) and head-neck offset ratio was smaller at the anterosuperior aspect (2 o'clock) than in the control group. The α-angle was significantly larger at the anterior aspects (1-4 o'clock) in the advanced group than in the pre/early group. The maximum α-angle was most commonly found at 2 o'clock (60%, 41/68 hips) in dysplastic hips. The prevalence of cam deformity (maximum α-angle >55°) was 4.2% (1/24 hips) in the control group, 22% (11/50 hips) in the pre/early group, and 50% (9/18 hips) in the advanced group. Cam deformity, inherent in the pre/early group, was found with relatively high frequency. The higher prevalence in the advanced group reflected degeneration-modified changes. When performing periacetabular osteotomy, preoperative radiographic assessments should include the femoral head-neck junction to prevent secondary femoroacetabular impingement, especially in patients with advanced stage osteoarthritis. This article is protected by copyright. All rights reserved.
    No preview · Article · Dec 2015 · Journal of Orthopaedic Research
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    ABSTRACT: Background Undifferentiated pleomorphic sarcoma (UPS) is a heterogeneous tumor group, and little is known about molecular target therapy for UPS. Heat shock protein 90 (HSP90) is an expressed chaperone that refolds certain denatured proteins under stress conditions. One of these proteins is Akt. The disruption of Akt signaling plays an important role in tumor progression. The present study’s purpose was to analyze the HSP90 expression, Akt/mTOR pathway activation and the correlation between HSP90 expression and its pathway activation in UPS. Methods The status of HSP90 and the profiles of the Akt/ mTOR pathway were assessed by immunohistochemistry in 79 samples of UPS, and these data were compared with clinicopathological and histopathological findings. The expressions of indicated proteins were assessed by Western blotting in five frozen samples. After treating UPS cells with the HSP90 inhibitor, we assessed the antitumor effect of the inhibitor. Results Immunohistochemically, phosphorylated Akt (p-Akt), p-mTOR, p-S6RP and p-4EBP were positive in 57.3, 51.9, 54.5 and 57.1 % of the UPS samples, respectively. The expressions of those phosphorylated proteins were correlated with each other. HSP90 expression was elevated in 56.4 % of the samples and was correlated with p-Akt, p-mTOR and p-S6RP. The immunohistochemical results were confirmed by Western blotting. The HSP90 inhibitor led to decreased viability and invasiveness of the cells and inactivated the AKT/mTOR pathway in vitro. Conclusion Elevated expression of HSP90 is a poor-prognosis factor and is involved in the activation of the Akt/mTOR pathway in UPS. HSP90 inhibition is a potential treatment option for UPS.
    Full-text · Article · Dec 2015 · BMC Cancer
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    ABSTRACT: Despite recent advancements in rheumatoid arthritis (RA) pharmacotherapy, surgeons still encounter severely damaged knees. The purpose of the present study was to analyze the mid-term clinical results of total knee arthroplasty (TKA) with metal block augmentation and stem extension. A total of 26 knees in 21 patients who underwent primary TKA with metal block augmentation and stem extension were retrospectively reviewed. All patients with a mean age of 63 years had RA for a mean duration of 15 years. Functional and radiographic results as well as complications were evaluated at the mean follow-up period of 6 years after TKA. Eight knees were lost follow-up after the two-year evaluation. Tibial bone defects with average depth of 19 mm were preoperatively recognized in all 26 knees. The postoperative joint line was reconstructed on average 11 mm above the fibular head using average thickness of 11 mm tibial inserts and 9 mm metal blocks with stem extension. Significant improvements (p < 0.05 for all comparisons) were observed postoperatively in maximum extension angle from -10° to -1°, range of motion from 101 ° to 115 °, and Knee Society Score (knee score/function score) from 35/18 to 90/64. Non-progressive radiolucent lines beneath the metal block and osteosclerotic changes around the medullary stem were found in 16 knees (62 %) and 14 knees (54 %), respectively. There was two failures (8 %): fragile supracondylar femur fractures and knee instability. No knees showed any radiographic implant loosening, dislocation, polyethylene insert breakage, peroneal palsy, or infection. Primary TKA with metal block augmentation and stem extension could effectively restore function in RA patients with advanced forms of knee joint destruction, and be reliable and durable for a mean postoperative period of 6 years. Further study is needed to determine the long-term results of TKA using metal block augmentation and stem extension.
    Full-text · Article · Dec 2015 · BMC Musculoskeletal Disorders
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    ABSTRACT: Background: Hydroxyapatite (HA) has been applied to joint prostheses as a bioactive coating to prolong their durability. However, HA benefits for cup survival in total hip arthroplasty (THA) remain controversial. In this study, we compared the survival of cups with and without HA coating during a minimum follow-up of 18 years. Methods: In total, 183 THA cases in 163 patients were analyzed, including 73 cups with HA coating (HA(+) group) and 110 without HA coating (HA(-) group); otherwise, the cups had identical titanium-sprayed rough surfaces and were fixed with screws. In both groups, the same conventional polyethylene liners were applied. Zirconia and alumina ceramic heads were used in the HA(+) and HA(-) groups, respectively. We retrospectively analyzed cup survival based on cup revision for any reason or aseptic loosening as end points. Results: In total, 7 and 8 revisions were performed in the HA(-) and HA(+) groups with survival rates of 86.3% and 90.1%, respectively. Among them, 3 cups in the HA(-) group and 1 cup in the HA(+) group were revised for aseptic loosening in 20 years (survival rates 94.1% and 98.7%, respectively). No significant difference was detected in cup survival rates between the groups based on both end points. There were no stem revisions during the observation period. Conclusion: The results suggested that HA coating did not have either beneficial or adverse effects on the long-term cup survival in primary cementless THA.
    No preview · Article · Dec 2015 · The Journal of arthroplasty
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    ABSTRACT: In patients with degenerative meniscal tears, subclinical cartilage degeneration may be present even if gross morphological changes are not evident. The aim of this study was to detect occult cartilage degeneration using T1ρ MRI mapping in patients with meniscal tears without obvious radiographic osteoarthritis (OA). A total of 22 subjects with degenerative meniscal tears in the early stages of osteoarthritis [Kellgren-Lawrence (KL) grade of 0-2] and 19 healthy subjects as the control group were examined. The femoral condyle was divided into four 30° wedges (-30°-0° anteriorly, 0°-30°, 30°-60° and 60°-90° posteriorly), and each area of cartilage was further divided into superficial and deep layers of equal thickness. The tibial side was divided into anterior and posterior areas with superficial and deep layers in each. The mean T1ρ values (ms) in each area were calculated. On the femoral side, T1ρ values of the superficial and deep regions (-30°-0°, 0°-30° and 30°-60°) in the meniscal tear group were significantly higher than those in the control group [superficial (-30°-0°): 49.0 ± 4.0 (meniscal tear group) vs 45.1 ± 2.1 (control group), deep (-30°-0°): 45.2 ± 3.3 vs 39.5 ± 5.0, superficial (0°-30°): 54.5 ± 5.3 vs 47.4 ± 5.7, deep (0°-30°): 46.8 ± 4.0 vs 40.7 ± 6.3, superficial (30°-60°): 50.5 ± 3.1 vs 47.1 ± 5.7]. On the tibial side, the meniscal tear group had significantly higher T1ρ values superficially in both anterior and posterior regions compared with the control group [superficial (anterior): 52.0 ± 4.3 vs 46.7 ± 5.4, superficial (posterior): 53.1 ± 5.1 vs 46.0 ± 4.9]. Moreover, these significant differences were observed when comparing patients in the meniscal tear group with KL grades of 0 or 1 and the control group. Our study suggested that early biochemical changes in cartilage associated with degenerative meniscal tears occur first in the superficial zones in areas of contact during slight flexion. Characterising the early relationship between cartilage degeneration and degenerative meniscal tears using T1ρ MRI mapping may be of clinical benefit and provide further evidence linking meniscal injury to OA.
    Full-text · Article · Dec 2015 · BMC Musculoskeletal Disorders
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    ABSTRACT: CCAAT/enhancer-binding protein β (C/EBPβ) is a transcription factor that is activated in the synovium in rheumatoid arthritis (RA) and promotes expression of various matrix metalloproteinases. In this study, we examined whether C/EBPβ mediates the expression of receptor activator of nuclear factor-kappa-B ligand (RANKL) and drives osteoclast formation in primary fibroblast-like synoviocytes (FLS) from RA patients. The cooperation of C/EBPβ and activation transcription factor-4 (ATF4) in the regulation of the RANKL promoter was also investigated. Immunofluorescence staining was performed for C/EBPβ, RANKL, and ATF4 in synovium from RA patients. Adenovirus expression vectors for two major isoforms, C/EBPβ-liver-enriched activator protein (LAP) and - liver-enriched inhibitory protein (LIP), or small interfering RNA for C/EBPβ, were used to manipulate C/EBPβ expression in RA-FLS. RA-FLS over-expressing C/EBPβ were co-cultured with peripheral blood mononuclear cells (PBMCs) to test osteoclast formation by tartrate-resistant acid phosphatase (TRAP) staining. A promoter assay for RANKL, a chromatin immunoprecipitation (ChIP) assay and an immunoprecipitation (IP) assay were also performed. Immunofluorescence staining showed colocalization of C/EBPβ, ATF4 and RANKL in RA synovium. Western blotting revealed the expression of C/EBPβ-LAP and -LIP in RA-FLS. Over-expression of either C/EBPβ-LAP or -LIP significantly increased the expression of RANKL mRNA, while C/EBPβ-LIP down-regulated osteoprotegerin (OPG) mRNA. The RANKL/OPG mRNA ratio was significantly increased by C/EBPβ-LIP over-expression. Knockdown of C/EBPβ with siRNA decreased the expression of RANKL mRNA. The number of TRAP-positive multinucleated cells was increased in co-cultures of PBMCs and FLS over-expressing either C/EBPβ-LAP or -LIP, but was more significant with LIP. C/EBPβ-LIP does not have a transactivation domain. However, promoter assays showed that C/EBPβ-LIP and ATF4 synergistically transactivate the RANKL promoter. ChIP and IP assays revealed the cooperative binding of C/EBPβ and ATF4 on the RANKL promoter. We demonstrated that C/EBPβ, especially C/EBPβ-LIP in cooperation with ATF4, is involved in osteoclast formation by regulating RANKL expression in RA-FLS. These findings suggest that C/EBPβ plays a crucial role in bone destruction in RA joints.
    Preview · Article · Dec 2015 · Arthritis Research & Therapy
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    ABSTRACT: Background: There is an interest in quantifying the hip kinematics of patients with end-stage hip disorders before total hip arthroplasty. The purpose of the present study was to obtain dynamic hip kinematics under four different conditions, including deep flexion and rotation, in patients with osteoarthritis of the hip. Methods: Continuous X-ray images were obtained in 14 patients during gait, chair-rising, squatting, and twisting, using a flat panel X-ray detector. These patients received computed tomography scan to generate virtual digitally reconstructed radiographs. The density-based digitally reconstructed radiographs were then compared with the serial X-ray images acquired using image correlations. These 3D-to-2D model-to-image registration techniques determined the 3D positions and orientations of the pelvis and femur during the movement cycle of each activity. Findings: For gait, chair-rising, and squatting, the maximum hip flexion angles averaged 22°, 64°, and 68°, respectively. The pelvis was tilted anteriorly by an average of around 7° during the full gait cycle. For chair-rising and squatting, the maximum absolute values of anterior/posterior pelvic tilt averaged 8°/17° and 6°/18°, respectively. Hip flexion showed maximum flexion angle on the way of movement due to further anterior pelvic tilt during both chair-rising and squatting. For twisting, the maximum absolute values of internal/external hip rotation averaged 3°/13°. Interpretation: Patients with hip osteoarthritis prior to total hip arthroplasty demonstrated the limited ranges of coordinated motion of the pelvis, femur, and hip joint during each activity, especially in deeply flexed and rotated postures.
    No preview · Article · Dec 2015 · Clinical biomechanics (Bristol, Avon)
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    ABSTRACT: No previous studies comparing the clinical wear rates of the two different kinds of cross-linked ultra-high-molecular-weight polyethylene (XLPE), annealed and remelted, are available. We compared the creep and steady wear rates of 36 matched pairs (72 hips in total) adjusting for baseline characteristics with propensity score matching techniques. Zirconia femoral heads with 26-mm diameter were used in all cases. The femoral-head cup penetration was measured digitally on radiographs. Significantly greater creep (p=0.006) was detected in the remelted (0.234mm) than annealed (0.159mm) XLPE. However, no significant difference (p=0.19) was found between the steady wear rates (0.003 and 0.008mm/year, respectively) of the annealed and remelted XLPE. Multiple regression analyses showed that remelted XLPE is significant independent variable (p<0.001) that is positively associated with creep. However, the patient age and body weight, cup size, the liner thickness, cup inclination, follow-up periods, and postoperative Merle d'Aubigné hip score had no significant effects (p>0.05) on the steady wear rates. No patients exhibited above the osteolysis threshold of 0.1mm/year, progressive radiolucencies, osteolysis, or polyethylene fracture. This propensity-matched cohort study document no significant difference in wear resistant performances of annealed and remelted XLPE over an average period of 10 years.
    No preview · Article · Dec 2015 · Journal of the Mechanical Behavior of Biomedical Materials
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    ABSTRACT: We present the case of an 80-year-old man with a tumor recurrence on his right arm 6 years after initial treatment. The lateral aspect of the elbow joint, involving overlaying skin, muscles, tendons, joint capsule, lateral collateral ligament complex, the lateral 1/3 of the capitellum, and lateral epicondyle of humerus were excised in the tumor resection. Intraoperative assessment revealed multidirectional instability of the elbow, and joint stabilization was needed. Because the lateral epicondyle was resected, graft placement in an anatomical position was impossible to carry out. Therefore, non-anatomical reconstruction of lateral ulnar collateral ligament with palmaris longus tendon graft was performed. The skin was reconstructed using an antegrade pedicled radial forearm flap. For wrist extension reconstruction, the pronator quadratus tendon was transferred to the extensor carpi radialis brevis tendon. One year after the operation, elbow range of motion was 5–130°. The patient remains symptom free. The Mayo elbow performance score is good. The Musculoskeletal Tumor Society rating score is excellent. To our knowledge, this is the first report of an elbow lateral ulnar collateral ligament reconstruction after tumor resection.
    Preview · Article · Nov 2015 · Strategies in Trauma and Limb Reconstruction
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    ABSTRACT: Leiomyosarcoma (LMS) of soft tissue is a sarcoma with smooth-muscle differentiation, and conventional chemotherapy does not improve its outcome. The application of novel antitumor agents and precise prognostication has been demanded. The expression of the protein Forkhead box M1 (FOXM1), a member of the FOX family, is considered an independent predictor of poor survival in many cancers and sarcomas. However, the expression status of FOXM1 in LMSs is poorly understood. The purposes of this study were to examine the correlation between the expression of FOXM1 and clinicopathologic or prognostic factors and to clarify the efficacy of FOXM1 target therapy in LMSs. We evaluated the immunohistochemical expressions of FOXM1 using 123 LMS tumor specimens. Univariate and multivariate survival analyses revealed that FOXM1 expression was associated with poor prognosis in LMS. An in vitro study was then conducted to examine the antitumor effect of a FOXM1 inhibitor (thiostrepton) and small interference RNA (siRNA) on a novel LMS cell line, TC616. We also assessed the efficacy of the combined use of doxorubicin and thiostrepton. Thiostrepton showed dose dependent antitumor activity and TC616 treated with the combination of thiostrepton and doxorubicin showed lower proliferation compared to those treated with either drug individually. FOXM1 interruption by siRNA decreased the cell proliferation and increased chemosensitivity. In conclusion, FOXM1 have a potential to be a therapeutic target for LMS. This article is protected by copyright. All rights reserved.
    No preview · Article · Nov 2015 · Cancer Science
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    ABSTRACT: Introduction: Corticosteroid-induced osteonecrosis of the femoral head (ONFH) often affects both femoral heads. Such bilateral ONFH cases are generally detected concurrently on magnetic resonance imaging (MRI). On the other hand, in unilateral cases, it is rare that contralateral ONFH is subsequently detected. We herein report a case in which bilateral ONFH was detected in both femoral heads by repeated MRI examination at an interval of 6 weeks. Case description: A 34-year-old man with purpura nephritis was started on corticosteroid therapy with prednisolone at 30 mg/day. Eight months after the initiation of corticosteroid therapy, he complained of left hip pain with no antecedent triggering activity. MRI obtained 8.5 months after the initiation of corticosteroid therapy showed the findings of osteonecrosis of the left femoral head, while no abnormalities were detected in the right femoral head. On the second MRI obtained 10 months after the initiation of corticosteroid treatment, however, osteonecrosis of the right femoral head was newly detected without an increase of the corticosteroid dose. Conclusions: This case may indicate that corticosteroid-induced bilateral ONFH do not always develop at the same time.
    Full-text · Article · Nov 2015 · SpringerPlus
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    ABSTRACT: The transcription factor Forkhead box M1 (FOXM1) is known to play critical roles in the development and progression of various types of cancer, but the clinical significance of FOXM1 expression in rhabdomyosarcoma (RMS) is unknown. This study aimed to determine the role of FOXM1 in RMS. We investigated the expression levels of FOXM1 and vascular endothelial growth factor (VEGF) and angiogenesis in a large series of RMS clinical cases using immunohistochemistry (n = 92), and we performed clinicopathologic and prognostic analyses. In vitro studies were conducted to examine the effect of FOXM1 knock-down on VEGF expression, cell proliferation, migration, and invasion in embryonal RMS (ERMS) and alveolar RMS (ARMS) cell lines, using small interference RNA (siRNA). High FOXM1 expression was significantly increased in the cases of ARMS, which has an adverse prognosis compared to ERMS (p = 0.0310). The ERMS patients with high FOXM1 expression (n = 25) had a significantly shorter survival than those with low FOXM1 expression (n = 24; p = 0.0310). FOXM1 expression was statistically correlated with VEGF expression in ERMS at the protein level as shown by immunohistochemistry and at the mRNA level by RT-PCR. The in vitro study demonstrated that VEGF mRNA levels were decreased in the FOXM1 siRNA-transfected ERMS and ARMS cells. FOXM1 knock-down resulted in a significant decrease of cell proliferation and migration in all four RMS cell lines and invasion in three of the four cell lines. Our results indicate that FOXM1 overexpression may be a prognostic factor of RMS and that FOXM1 may be a promising therapeutic target for the inhibition of RMS progression.
    No preview · Article · Nov 2015 · Tumor Biology
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    ABSTRACT: Study design: A computed tomography study. Objective: To clarify the position of the aorta relative to the spine in patients with Lenke type 1 adolescent idiopathic scoliosis. Summary of background data: Several authors have examined the position of the aorta in patients with scoliosis. However, their analysis included several types of curve. There is a possibility that the position of the aorta differs according to the scoliosis curve type. Methods: Thirty-eight patients with Lenke type 1 were analyzed. The angle (LtP-Ao angle) and distance (LtP-Ao distance) from the insertion point of the left pedicle screw to the aorta were measured from T4 through L2. The measured data were evaluated from four levels above to four levels below the apical vertebra. The difference between lumbar modifiers A and C was examined. Dangerous pedicles, which were defined as those in which the aorta entered the expected area based on the screw direction error and length, were counted from T10 to L2. Results: The aorta located posterolaterally and adjacent to the vertebra at the middle thoracic level, and anteromedially and distant at the thoracolumbar level. LtP-Ao angle was largest at one level above the apical vertebra, and LtP-Ao distance was shortest at two levels above. LtP-Ao angle of Lenke 1A was significantly larger than 1C from T11 to L2, and LtP-Ao distance of 1A was significantly shorter than 1C from T11 to L1. When the screw length was 40 mm and the direction error was within 10°, there were a large number of dangerous pedicles at T11, regardless of the lumbar modifier. Conclusions: The direction error has a potential risk of injuring the aorta around the apical vertebra. The selection of screws of the proper length is necessary to avoid a breach of the anterior vertebral wall at thoracolumbar level, especially at T11. Level of evidence: 3.
    No preview · Article · Nov 2015 · Spine

Publication Stats

12k Citations
1,947.32 Total Impact Points


  • 1989-2016
    • Kyushu University
      • Department of Orthopaedic Surgery
      Hukuoka, Fukuoka, Japan
  • 2014
    • Saga University
      • Department of Advanced Technology Fusion
      Сага Япония, Saga Prefecture, Japan
  • 2013
    • Osaka City University
      Ōsaka, Ōsaka, Japan
  • 2011
    • Niigata University
      Niahi-niigata, Niigata, Japan
    • Indiana University-Purdue University Indianapolis
      • Department of Medicine
      Indianapolis, Indiana, United States
  • 2001-2009
    • Kyushu Sangyo University
      Hukuoka, Fukuoka, Japan
  • 2008
    • Hospital for Special Surgery
      New York, New York, United States
  • 2001-2007
    • Fukuoka University
      • Department of Orthopaedic Surgery
      Hukuoka, Fukuoka, Japan
  • 2006
    • National Institute of Allergy and Infectious Diseases
      Maryland, United States
  • 2004
    • National Cancer Center, Japan
      Edo, Tōkyō, Japan
    • Kitakyushu University
      • Faculty of Environmental Engineering
      Kitakyūshū, Fukuoka-ken, Japan
  • 2003
    • Bryn Mawr College
      Bryn Mawr, Pennsylvania, United States
  • 1998
    • Kitasato University
      • Department of Orthopedic Surgery
      Edo, Tokyo, Japan