[Show abstract][Hide abstract] ABSTRACT: In an acute kidney transplant rejection rat model, we demonstrated that manganese superoxide dismutase (MnSOD) activity was significantly reduced and MnSOD was nitrated by peroxynitrite (ONOO(-)), resulting in tissue injury. We examined whether tissue injury was reduced after external supplementation of recombinant human MnSOD in a rat renal ischemia-reperfusion injury model.
Male Brown-Norway rats underwent dissection of the right kidney. The animals were divided into 3 groups. The controls had the left renal blood vessels clamped for 90 minutes to induce ischemia, followed by reperfusion for 16 hours. In the intraperitoneal administration group, MnSOD was administered 30 minutes before ischemia and immediately before reperfusion. In the sham group, neither ischemia nor reperfusion was performed. After reperfusion, blood was collected, the left kidney was dissected and renal function and tissue injury were evaluated.
Serum creatinine and K(+), blood urea nitrogen, and aspartate aminotransferase activity decreased significantly, whereas serum Na(+) and renal function improved in the MnSOD group compared with the control and sham groups. On hematoxylin and eosin staining, the histological score indicated that acute tubular necrosis was significantly reduced by MnSOD administration. Periodic acid-Schiff staining was absent in the nonadministration group, whereas it persisted in the MnSOD group. In the proximal renal tubules a large proportion of anti-nitrotyrosine staining was present before but absent after MnSOD administration.
MnSOD administration improved renal function and reduced tissue injury. It may also reduce tissue injury in acute kidney transplant rejection and other tissue injuries caused by similar molecular mechanisms.
No preview · Article · Nov 2009 · Transplantation Proceedings
[Show abstract][Hide abstract] ABSTRACT: AIM: Intravesical Bacillus Calmette-Guérin (BCG) therapy is an effective treatment for superficial bladder cancer. However, its frequent and severe side-effects were major obstacles for clinical setting. In this study, BCG was fractionized with simple methods to find an active component. METHODS: Sonicated or autoclaved Tokyo 172 BCG strain was fractionated to supernatant and precipitate. These fractions were co-cultured with 5637 human bladder cancer cell line (5637 cell). The growth inhibitory effect on 5637 cell was analyzed by dye exclusion test, 3H-thymidine incorporation, cytologic examination with Giemsa staining, and cell cycle analysis using flowcytometry. RESULTS: Live BCG and supernatant fractions obtained by sonication or autoclave suppressed the growth of 5637 cell. The dark-stained spots suggesting early phase apoptosis were found in nuclei of 5637 cells by co-cultured with live BCG or supernatant fractions. In these cells, the ratios of apoptotic cells were increased compared with non-treated cells. CONCLUSION: These results suggest that BCG has a direct anti-tumor effect to induce apoptosis on 5637 cell. Supernatant fractions obtained by sonication or autoclave maintained the direct anti-tumor effects as well as live BCG. Further purification of these fractions may provide a new BCG derivatives which has higher efficacy with reduced toxicity.
No preview · Article · Jan 2007 · Hirosaki Medical Journal
[Show abstract][Hide abstract] ABSTRACT: A 59-year-old woman was admitted to our hospital because of a mass lesion in the retroperitoneal space found by follow-up CT. She had undergone resection of a perinephric liposarcoma and had received adjuvant chemotherapy (CYVADIC therapy: vincristine, adriamycin, cyclophosphamide and dacarbazine) for 2 years and 1 month. Abdominal computed tomography revealed a low-density mass with a fibrous capsule at the same location. The probable diagnosis was recurrent liposarcoma arising in the perinephric retroperitoneum. Left nephrectomy and resection of the left retroperitoneal mass were performed. The removed specimen weighed 130 g and measured 7.5 × 5.5 × 5 cm. Histological examination of the tumor showed well differentiated liposarcoma and no invasion of the kidney. She received no adjuvant therapy, and no metastasis or recurrence has been detected during 13 months of postoperative follow-up.
No preview · Article · Jan 2003 · Nishinihon Journal of Urology
[Show abstract][Hide abstract] ABSTRACT: A 44-year-old female was referred to our hospital with the chief complaint of difficult urination. Cystoscopic examination disclosed irregular mucosa of the urethra at 6 O'clock. Pathological diagnosis was moderately-differenciated adenocarcinorna. MRI showed a high intensity mass on T2 weighted image. Total cystectomy and ileal conduit formation was performed. Pathological and histochemical studies of the surgical specimen revealed moderately-differenciated adenocarcinoma which was positive for carcinoembryonic antigen (CEA). She underwent adjuvant chemotherapy with EPI, CDDP and 5-FU. The patient is alive with no evidence of any reccurence at 12 months after the surgery.
No preview · Article · Jan 2002 · Japanese Journal of Clinical Urology
[Show abstract][Hide abstract] ABSTRACT: Twenty patients with renal cell carcinoma (RCC), comprising 16 elective cases and 4 imperative cases, underwent partial nephrectomy at Hirosaki University Hospital between 1998 and 2001. The median maximum diameter of the tumors was 2.0 cm in the elective cases and 4.5 cm in the imperative cases. Severe complications due to the operation was the renal infarction in one case, and the postoperative elevation of serum creatinine was transient in the elective cases. The median follow-up periods were 14 months in both groups. None of the elective cases had either local recurrent tumors or metastatic tumors, but 2 of the imperative cases died at 5 and 12 months after surgery, respectively. Nephron-sparing surgery is believed to result in excellent control for low-grade, low-stage tumors.
No preview · Article · Jan 2002 · Nishinihon Journal of Urology
[Show abstract][Hide abstract] ABSTRACT: A 69-year-old woman complaining of pollakisuria was referred to our hospital for retroperitoneal tumor revealed with ultrasonography. Abdominal computed tomography showed a low density tumor over the right kidney and the inferior vena cava (IVC) was remarkably compressed, suggesting the invasion to IVC by the tumor. On arteriography, it was hypervascular and was fed by the middle and lower adrenal arteries. Inferior venacavography showed remarkably compressed of IVC by the tumor. Although hormonal examination showed normal, the right adrenal adenocarcinoma was suspected. The tumor was resected together with the right kidney, the right adrenal gland and the part of IVC. Histopathological diagnosis was leiomyosarcoma without any invasion to the kidney or adrenal gland.
[Show abstract][Hide abstract] ABSTRACT: We report a case of asynchronous right renal cell carcinoma and left hemorrhagic renal cyst in acquired cystic disease of the kidneys. The patient was a 44-year-old man with a 13-year history of hemodialysis and ultrasonic examinations revealed a tumor arising from the Cystic wall in the right kidney. Right nephrectomy was performed and the histological diagnosis was renal cell carcinoma. During follow-up, computed tomography showed a high- density area within the cyst at the upper pole in the remnant kidney and ultrasonic examinations revealed a tumor arising from the cystic wall. Left nephrectomy was performed and pathological examinations showed a hemorrhagic cyst without malignancy.
No preview · Article · Jun 2000 · Nishinihon Journal of Urology
[Show abstract][Hide abstract] ABSTRACT: A 73-year old male presented with gross hematuria. The laboratory studies revealed remarkable leukocytosis of 16,700/mm3 (neutrophils, 83%) in the peripheral blood. Serum analysis revealed a high level of granulocyte colony-stimulating factor (G-CSF), 149pg/ml. Cystoscopy, CT and MRI demonstrated a large bladder tumor. The patient underwent total cystectomy and construction of ileal conduit. Histological diagnosis was undifferentiated carcinoma. After surgery, WBC count in the peripheral blood and serum G-CSF concentration decreased within normal range. This is the 20th reported case of G-CSF producting bladder tumor in Japan.
[Show abstract][Hide abstract] ABSTRACT: We report 6 cases of cystic renal cell carcinoma (RCC). Tumor was considered to originate within simple renal cyst in 4 cases, and to be multilocular cystic type in 2 cases. All cases were diagnosed preoperatively as malignant neoplasms with radiological examinations, including CT, MRI and angiography. Radical nephrectomy were performed in 5 cases and partial nephrectomy in one case. Histological diagnosis revealed that RCC occurred within simple renal cyst because of hobnail cells in the renal cystic wall in 4 cases, and mulcilocular cystic RCC in 2 cases. Nephron sparing surgery as an option of the management of cystic RCC was recommended because cystic RCCs are generally low grade and enveloped by distinct fibrous pseudocapsules.
No preview · Article · Oct 1999 · Japanese Journal of Clinical Urology