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Publications (10)1.05 Total impact

  • U.R. Mallu · V. Bobbarala · S. Penumajji
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    ABSTRACT: A novel and single RP-HPLC method was developed for the determination of ten active ingredients (Codeine phosphate, Paracetamol, Chloropheniramine Maleate, Theophylline, Pseudoepidrine HCl, Ambroxol, Salbutamol, Guaiphenesin, Dextromethorphan and Diphenhydramine HCl) in all pharmaceutical dosage forms, along with preservative (Sodium benzoate) and validated the method as per ICH and FDA guidelines. The separation was achieved on a X-Terra C18, 15cm × 4.6mm, 3.5 μ in the simple gradient mode using Sol-A: buffer and Sol-B: Acetonitrile (0-5min, sol-A:97-97; 5-10min- sol-A:97-92; 10-15min- sol-A:92- 68; 15-23min- sol-A:68-68; 23-25min- sol-A:68-97 and 25-30min- sol-A:97-97) with 0.8 mL per min flow rate. Column oven temperature maintained at 40°C and performed the analysis with 220 nm. Quantification was achieved with 40μg per mL for all ingredients with 100±3.0% recovery. The method was validated by determining its sensitivity, linearity, accuracy and precision. The proposed method is single, shorter runtime, accurate and reproducible. This method can be applied for routine analysis of all ten active ingredients quantification in all pharmaceutical dosage forms.
    No preview · Article · Jan 2011 · International Journal of Pharma and Bio Sciences
  • U.R. Mallu · K. Hussain Reddy · V. Bobbarala · S. Penumajji
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    ABSTRACT: The aim of this study was to develop a RP-HPLC method for determining the contents of beclomethasone dipropionate, clotrimazole, chloramphenicol and lidocaine in ear drops formulation. Chromatographic separation was achieved on a Octa-decyl silane (C18) column with a simple gradient program, mobile phase is Sol-A: buffer ( 1.6 g of CH 3COONH 2 into 1000mL of HPLC grade water, add 10mL o f TEA and adjust the pH to 6.4± 0.1 with diluted acetic acid) and Sol-B: Acetonitrile. 1.0ml per min flow rate and detection was at 254 nm. Chloramphenicol, lidocaine, belclomethasone dipropionate and clotrimazole were eluted at 5.0min, 10.8min, 16.2min and 19.1min, respectively. The absorbance of four active peaks was a linear function of concentration in the range 1.0ppm to 6.0ppm for Beclomethasone Dipropionate, 187.5ppm to 1125.0ppm for Chloramphenicol, 37.5ppm to 225.0ppm for Clotrimazole and 65ppm to 390ppm for Lidocaine, correlation coefficients of Beclomethasone Dipropionate is 0.9997, Chloramphenicol is 0.9999, Clotrimazole is 0.9998 and Lidocaine is 0.9999 respectively. The reverse-phase HPLC assay method was developed and validated. The method has wide applicability for these four active ingredients. The method has reproducibility, accuracy and robust.
    No preview · Article · Jan 2011 · International Journal of Pharma and Bio Sciences
  • S Penumajji · V Bobbarala · V Vadlapudi · K Naidu
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    ABSTRACT: Dried whole plant of Vitexagnus cactus was extracted with organic solvents in different ratios and analyzed using high performance liquid chromatography (HPLC) with evaporative light scattering (ELS) detector. The markers of interest in Vitexagnus castus were agnuside, casticin and vitexyl acetone. These standard molecular markers do not contain strong chromophores, hence it is difficult to identify them using the UV detection system in high performance liquid chromatography. ELS detector is regarded as a valuable alternative to UV detection system for identification of the compounds that do not contain strong chromophores. The results of the present study show that the ELS detection system is more efficient than the UV-visible detection system.
    No preview · Article · Sep 2010 · West African journal of pharmacology and drug research
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    ABSTRACT: The aim of this study is to development and validation of a stability indicating RP-HPLC method for the determination of dissolution and assay of Sildenafil citrate tablets 50mg and 100mg using RP-HPLC with UV detector. Sildenafil citrate and related substances were baseline separated and quantitated on a C18 reverse phase column (4.6mm × 250mm, 5.0µm), using a mobile phase composed of a phosphate buffer-acetonitrile (40:60v/v,) delivered at a flow rate of 1.0mL/min, and with UV detection (lexcitation = 225nm). The method was proven to be linear over a Sildenafil citrate concentration range of 13 to 83µg/mL with a mean correlation coefficient of 0.9999.
    Full-text · Article · Jan 2010
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    ABSTRACT: Asparagus racemosus is known as Shatavari in Sanskrit. Dried roots of A. racemosus were extracted with organic solvents in different ratios and were analyzed using high performance liquid chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD). The markers of our interest in A. racemosus are Shatavari I and Shatavari IV. As both the markers do not contain strong chromophore, it is difficult to identify it in the UV detection system of High performance liquid chromatography. Our research reports suggested that High Performance Liquid Chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD) is used as an alternative and effective tool for the identification of the compounds which do not contain chromophores. Present study concludes that ELS Detection system is more efficient than the UV/Vis detection system.
    Full-text · Article · Jan 2010
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    ABSTRACT: Amiodarone is an anti-arrhythmic agent used for various types of tachyarrhythmia both ventricular and supra ventricular arrhythmias (fast forms of irregular heart beat). Present study is aimed to develop simple, specific, sensitive, and rapid high performance liquid chromatography (HPLC) method for the determination of Amiodarone hydrochloride. Amiodarone was identified and quantitated on a C2 reversed phase column (4.6 × 250mm, 5.0µ), using a mobile phase composed of acetate buffer-Acetonitrile (15:85v/v) delivered at a flow rate of 1.0 mL/min, and with UV detection (lamda excitation= 240 nm). The method was proven to be linear over Amiodarone hydrochloride concentration range of 12.5 to 75µg/mL with a mean correlation coefficient of 0.9999. The present study is the development of HPLC method for the Pharmaceutical Formulation of Amiodarone Hydrochloride.
    Full-text · Article · Jan 2010
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    ABSTRACT: In this present study forty nine different plants used in traditional Indian medicine were examined against Aspergillus niger using agar well diffusion method. The methanolic extracts of 43 plants exhibited varying degrees of inhibition activity against the fungi. Among the forty nine plants studied 86% of the plants had antifungal activity while the remaining 14% had no antifungal activity. The extract from Grewia arborea showed maximum activity. Emblica officinales, Heldigordia populipolia, Hyptis sueolences, Moringa heterophylla, Strychnos nuxvomica and Vitex negundo did not exhibit antifungal activity at the condition studied.
    Full-text · Article · Apr 2009 · Indian Journal of Science and Technology
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    ABSTRACT: Vitex agnus castus extracts in different solvent ratios were analyzed using High Performance Liquid Chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD). The markers of our interest in Vitexagnus castus are Agnuside, Casticin and Vitexyl acetone. To correlate the retention time of the molecular marker compounds with Vitex agnus castus extract were compared with standard molecular markers of Agnuside, Casticin and Vitexyl acetone using same conditions of that of extract and were analyzed statistically. Apart from the results ELS Detector is regarded as a valuable alternative to UV detection system for liquid chromatographic analysis of substances that doesnot contain a strong chromophore. As the markers do not contain strong chromophore, it is difficult to identify it UV detection system in High performance liquid chromatography. So an alternative is the Evaporative Light scattering Detector which is an effective tool for the identification of the compounds which do not contain chromophores. Present study concludes that ELS Detection system is more efficient than the UV/Vis detection system.
    Full-text · Article · Jan 2009
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    ABSTRACT: Excoecaria agallocha L. leaves were extracted by various extracting procedures, using different solvents for testing the antimicrobial activities against important microorganisms using agar well diffusion method. Chloroform and methanolic extracts were found to be effective against these organisms, whereas hexane extracts were inactive. The purpose of this study was to find preliminary data for the development of alternative treatments to chemical microbicides for the control of plant diseases from natural plant extracts.
    Full-text · Article · Jan 2009 · International Journal of ChemTech Research
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    ABSTRACT: The antifungal activity of forty nine medicinal plants belonging to different families was tested in vitro on phytopathogenic fungusColletotrichum graminicola. In which methanolic extracts of forty two plants exhibited varying degrees of inhibition activity against C.graminicola. The results revealed that extract of Terminalia chebula was highly effective in inhibiting the mycelial growth of C. graminicola at 75µg/50µL. The following six plants Acalypha indica, Eichhornia crassipes, Gyanandropsis gyanandra, Suaeda maritime, Tephrosia pumila and Tinospora cordifolia did not exhibit antifungal activity.
    No preview · Article · Jan 2009