Snežana Babić

Institut Za Krmno Bilje, Kruševac, Central Serbia, Serbia

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Publications (19)9.11 Total impact

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    ABSTRACT: Alfalfa (Medicago sativa L.) is the most important legume that, in addition to high yield potential and quality of biomass, is characterized by an intense process of biological nitrogen fixation. Various microorganisms that can have a positive or negative effect on plant development are present in the rhizosphere of alfalfa. Certain rhizosphere microorganisms such as Sinorhizobium meliloti and Azotobacter chroococcum have nitrogen-fixing role and the role of bio-stimulators. But in addition to beneficial microorganisms, phytopathogen fungi can also occur. The experiment was carried out in vegetation pots in semi-controlled conditions at the Institute for Forage Crops in Kruševac. Before sowing, the seed of alfalfa and soil was inoculated with Azotobacter chroococcum and Sinorhizobium meliloti). The plants were mown after sixseven weeks and thereafter treated with Colletotrichum trifolii (Coll-4 isolates) conidia. The effect of inoculation was determined at the end of the vegetation period. This paper presents the results of the impact of microbial inoculants with two nitrogen-fixing bacteria (Sinorhizobium meliloti and Azotobacter chroococcum) and one isolate of the phytopathogen fungus Colletotrichum trifolii (Coll-4) on the number aminoheterotrophs in the rhizospheric soil of alfalfa varieties (Affinity, Perry and K-28). Results of Fisher test shows the absence of statistically significant differences in abundance of aminoheterotrophs between treatments, not recorded the existence of homogenous groups. The highest number of aminoheterotrophs was determined in rhizosphere of cultivar K-28 which was inoculated with Colletotrichum trifolii and Sinorhizobium meliloti. while inoculation with these microorganisms the lowest number was found in rhizosphere of cultivar Affinity. The number of aminoheterotrophs depended on the varieties of alfalfa and applied microbial inoculant, so that there is diversity among treatments. There is no clear trend influence of any of the factors applied. Keywords: rhizosphere, fungi, aminoheterotrophs, alfalfa. ACKNOWLEDGMENT Research was financed by the Ministry of Education, Science and Tehnological Development, Republic of Serbia, project TR 31057.
    Full-text · Conference Paper · Sep 2015
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    Preview · Article · Jan 2015
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    ABSTRACT: The objective of this study was to test the general combining ability (GCA) of different alfalfa varieties, with the main objective being to improve local varieties through the selection of good parental gene donors. Foreign varieties that in previous studies had been found to adapt well to agro-ecological conditions in Serbia were selected as possible gene donors. The female parental lines from domestic cvs K 22 and K 28 were crossed with the males of seven different alfalfa varieties. Progenies from the crosses were sown in nursery and observed during the second and third year of growth in the experimental field at the Institute for Forage Crops, Krusevac. Results showed significant differences between progenies. Variation among progenies for forage yield and forage yield components (plant height and number of stems per plant) were primarily attributed to general combining effects. The variety Magna had the best GCA with all domestic varieties for forage yield and was an excellent donor for improving this trait. Cultivars Integrity and Magna had the best GCA for increasing the number of stems per plant. These varieties demonstrated a favourable genetic potential that could contribute to the improvement of alfalfa yield in our breeding program. Varieties Vali and Pointer showed negative results for all traits and their poor GCA indicated that they are not suitable for improving domestic selection material. The best progeny will be used as parental lines in a new synthetic variety.
    No preview · Chapter · Jan 2014
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    ABSTRACT: Tetraploid cultivars of Italian ryegrass have been developed and used as an alternative to diploid cultivars in an attempt to improve forage yield and quality. But, very often, production of tetraploid cultivars is reduced by unfavourable weather conditions. The objective of this research was to determine seed production stability of the tetraploid Italian ryegrass cultivar K29T in variable and contrasting environmental and meteorological conditions in Serbia. The study was conducted in four consecutive years and generations of seed multiplication. Every year, seed was harvested from the primary growth in the first production year after the year of establishment. Data of investigated traits (heading and harvest dates, tiller length, number of spikelets per spike, seed yield, dry herbage yield and 1,000 seed weight) were recorded for different management practices (stand densities and nitrogen applications). It was determined that seed yield, dry matter production and tiller length were presumably most affected by different environmental factors in different production years, and different management practice, especially different sowing densities. In contrast, number of spikelets per spike and 1,000 seed weight were less affected by management practice, except in the extremely arid 2003 year. Variation in seed yield was more related to variation in seed numbers than to variation in seed weight. In the first seed production year, the best density in this experiment was 15–20 kg ha−1 of seed sown at an interrow spacing of 60 cm without fertilizing or with only 50 kg ha−1 of N. Therefore, seed of tetraploid Italian ryegrass cv. K29T can be produced in diverse environmental conditions in Serbia, but lower stand densities allow for better plant tillering and the production of a greater volume of seed and, at the same time, good dry matter yield of the residual biomass.
    No preview · Chapter · Jan 2014
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    ABSTRACT: In this study, the influence of pre-sowing inoculation with individual and combined cultures of rhizobium (Rhizobium meliloti), azotobacter (Azotobacter chroococcum) and actinomycetes (Streptomyces spp.) on growth parameters of alfalfa plants (height, number of stems and plant weight) was investigated. The mass of plant root was also determined. Analyses were carried out at the onset of flowering. The experiment was a two-factorial design, where the growth of plants influenced by seven variants of microbial inoculation was compared with the growth of non-inoculated controls for two alfalfa cultivars (K-28 and Syntéza 1). The experiment was carried out in pots in semi-controlled conditions. For this study, plants were sown on acid soil with pH 4.7. For tested parameters, interaction between cultivar and inoculation resulted in a positive effect compared to the control. Highest plant height was achieved in cv. Syntéza 1 using an inoculum that contained all of the species of microorganisms. By using this microbial variant, the highest value for green mass and number of stems per plant was achieved in cv. K-28. In this cultivar, using the combined cultures of rhizobium and actinomycetes, the highest value for root mass was achieved.
    No preview · Chapter · Jan 2014
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    ABSTRACT: Meadow fescue (Festuca pratensis Huds.) is an important perennial grass for animal feed production on grasslands and pastures. A basic requirement for successful breeding of this species is variability of the initial material. The collection examined consisted of 11 accessions (five breeding populations and six cultivars) of meadow fescue. The trial was conducted as a spaced plant nursery with 30 plants per genotype over 2 years. The aim was to determine genetic variability of the most important traits, specifically heading date (phenological trait), plant height in the first and the second cut, first internodes length, the number of tillers per plant and length, width and number of leaves, (morphological traits), as well as dry matter yield per plant. Statistical analysis of the data (ANOVA) revealed significant variability of genotypes for all properties included in the study. The highest average within-population variability was found for the number of tillers (CV 58 %), while the lowest variability was found for heading date (CV 8.8 %). The between-population variability was statistically significant for all investigated traits. Cluster analysis was used to graphically illustrate the genetic diversity of the meadow fescue accessions included in the study. The high level of genetic variability of this collection highlights the potential for future success in the breeding of meadow fescue.
    No preview · Chapter · Jan 2014
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    ABSTRACT: Seed production is a critically important trait in alfalfa breeding, and understanding the relationship between pollinator visitation and seed yield could help the development of high seed-yielding cultivars. The effect of three alfalfa cultivars on pollinator visit, seed yield and seed yield components was researched. To determine visitation rates, pollinators within a 1 m2 area were counted during a 30 min period in the morning, at noon and in the afternoon. Most pollinators were attracted to cv. K-28 (13.2). Cultivar NS Medijana was visited by 9.81 pollinators and cv. K-22 by 9.06. Cultivar K-28 attracted the highest number of honey bees (40.1 %), followed by cv. NS Medijana (31.3 %) and cv. K-22 (28.6 %). The ratio of honeybees to other observed pollinators was approximately 3:1. Cultivar K-28 achieved a seed yield of 553.3 kg ha−1, cv. Medijana 472.8 kg ha−1 and the seed yield of cv. K-22 was 469.4 kg ha−1. Cultivar K-22 had the most flowers and fertilized pods in the middle and highest inflorescences as well as seeds per pod in the highest inflorescences. Cultivar Medijana had the most flowers on the lowest inflorescences. Cultivar K-28 showed the highest values for other observed traits. Cultivar K-28 showed very significant differences from the other varieties in percentage of fertilized pods at the highest inflorescences. In relation to seed yield, numbers of flowers, pods and seeds per pod at the lowest inflorescences, cv. K-28 was significantly better than other cultivars, which positively correlated with the number of visiting pollinators. For other yield components, differences between cultivars were not statistically significant.
    No preview · Chapter · Jan 2014

  • No preview · Article · Dec 2013
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    ABSTRACT: ABSTRACT Alfalfa (Medicago sativa L.) is the most important legume that, in addition to high yield potential and quality of biomass, is characterized by an intense process of biological nitrogen fixation. Various microorganisms that can have a positive or negative effect on plant development are present in the rhizosphere of alfalfa. Certain rhizosphere microorganisms such as Rhizobium meliloti and Azotobacter chroococcum have nitrogen-fixing role and the role of bio-stimulators. But in addition to beneficial microorganisms, phytopathogen fungi can also occur. The aim of the research was to investigate the effect of inoculating alfalfa with two nitrogen-fixing bacteria (Azotobacter chroococcum and Rhizobium meliloti) and two species of the phytopathogen fungus Colletotrichum (Colletotrichum trifolii and Colletotrichum destructivum) on the number fungi and actinomycetes in the alfalfa rhizosphere. The highest number of fungi was determined in the treatment of inoculation with C. destructivum (isolate CC 657) and A. chroococcum, while the lowest number was recorded in treatment where C. trifolii (isolate Coll-4) was applied. In the control treatment (without inoculation) there was the highest number of actinomycetes, and the lowest abundance of these microorganisms was determined in the treatment with C. destructivum (isolate CC 657) and R. meliloti inoculation. Keywords: rhizosphere, fungi, actinomycetes, nitrogen-fixing bacteria, phytopathogen fungi
    No preview · Conference Paper · Sep 2013
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    ABSTRACT: ABSTRACT Alfalfa (Medicago sativa L.) is the most important legume that, in addition to high yield potential and quality of biomass, is characterized by an intense process of biological nitrogen fixation. Various microorganisms that can have a positive or negative effect on plant development are present in the rhizosphere of alfalfa. Certain rhizosphere microorganisms such as Rhizobium meliloti and Azotobacter chroococcum have nitrogen-fixing role and the role of bio-stimulators. But in addition to beneficial microorganisms, phytopathogen fungi can also occur. The aim of the research was to investigate the effect of inoculating alfalfa with two nitrogen-fixing bacteria (Azotobacter chroococcum and Rhizobium meliloti) and two species of the phytopathogen fungus Colletotrichum (Colletotrichum trifolii and Colletotrichum destructivum) on the number fungi and actinomycetes in the alfalfa rhizosphere. The highest number of fungi was determined in the treatment of inoculation with C. destructivum (isolate CC 657) and A. chroococcum, while the lowest number was recorded in treatment where C. trifolii (isolate Coll-4) was applied. In the control treatment (without inoculation) there was the highest number of actinomycetes, and the lowest abundance of these microorganisms was determined in the treatment with C. destructivum (isolate CC 657) and R. meliloti inoculation. Keywords: rhizosphere, fungi, actinomycetes, nitrogen-fixing bacteria, phytopathogen fungi
    Full-text · Conference Paper · Sep 2013

  • No preview · Article · Aug 2013 · Alcohol and Alcoholism
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    ABSTRACT: The identification and breeding of genotypes with improved drought tolerance play an important role in developing grasses with better performance and persistence. Plants should maintain some root contact with ground water to maintain cell turgor and to survive during drought periods. Plants with longer roots can better exploit the available, deeper, soil water. Breeding idea is to increase soil water uptake and drought tolerance of perennial ryegrass by improving root architecture with preserved above ground biomass yield. The objectives of this study were to determine variability of root dept and distribution, shoot dry matter yield (DMY) and root/shoot ratio of three Lolium perenne cultivars contrasting in filed persistency. The trial was conducted in 0.9 m sand plastic tubes with 30 plants per cultivar in three replications. Plants were irrigated daily with complete nutrient solution and trimmed once after 80 days of growth. The roots were washed out three months after planting, cut in 10 cm increments, dried and weighed. Data were analyzed by standard ANOVA. There were significant differences between cultivars and those with better persistency showed higher proportions of deep roots and 8 % heavier roots in total. Similar root/shoot ratios for all cultivars have showed that plants which have invested more dry matter into root have not automatically had less shoot yield. Genotypes with deeper root systems can be used for drought resistance improvement in Lolium perenne.
    No preview · Chapter · Jan 2013

  • No preview · Conference Paper · Jan 2013
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    ABSTRACT: In order to improve productive traits of soil and production potential of plants, microbial inoculation is increasingly being applied. In these studies, the effect of inoculation with rhizobium, аzotobacter and аctinomycetes, individually and in combinations, on the abundance of azotobacter in the rhizosphere of alfalfa grown on soils of different pH values was examined. Alfalfa seed was inoculated with the following inoculants: rhizobium (Rhizobium meliloti), azotobacter (Azotobacter chroоcoccum) and actinomycetes (Streptomyces sp.). These microorganisms are useful as individual and combined cultures (a combination of two or all three types of microorganisms). The control variant was without inoculation. After inoculation, the seeds were sown in each variant in vessels filled with soil (semi-controlled conditions). After the first cutting of alfalfa, the number of аzotobacter was determined in the rhizosphere of alfalfa in each treatment. The number of аzotobacter was significantly different in the rhizosphere of alfalfa grown on each of the two soil types for all inoculation treatments when compared to the control variant.
    No preview · Conference Paper · Jun 2012
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    ABSTRACT: Introduction Achondroplasio is a genetically induced disease, by a mutation on FGFR3 gen. Unproportionaly small height, short extremities caused by failure of enchondrial osteogenesis. Frequency is 1:25000 deliveries. Autosomal dominant hereditary disease. Case report Mail neonate born on 02/03/2010. in our Clinic, as a first child from a first uncontrolled pregnancy of a mother with achondroplasio, father-healthy. Parents both mother and father’s-healthy. Delivery by CS: Hondrodysplasio (diagnosed 30 days before the delivery). Head disproportionally bigger, forehead protuberant, nose root subsided, sutures 1cm in diameter. Proximal parts of extremities much shorter (rhisomelia). Restricted excursions in elbow and knee. Fingers much shorter and thicker. Other systems-normal. Brain ultrasound-normal. Genetically check up-Achondroplasio neonati. Discussion Achondroplasio is a hereditary disease recognized after birth. Without hydrocephalus life expectancy, mental and sex development is normal. Therapy options are surgical treatment and hormone supplementation. Cervical and lumbal spinal stenosis, gives neurological, respiratory problems and anesthesiological problems. Strabismus, conductive hearing failure demands ophthalmological and auditive care. Complication prevention is the base of all therapy treatments including a large team: neonatologist, clinical geneticist, pediatrician, neurologist, otorinolaringologist, ophthalmologist, pulmologist, endocrinologist, anesthesiologist, surgeon, psychiatrist, and psychologist. Recurrence risk is over 50%. As prenatal diagnostic is available, to prevent the risk of recurrence, individuals with achondroplasio, motivated to have healthy descendants, during pregnancy planning, are obligated to seek a genetical supervision and thorough pregnancy control. Main mother question: Is it going to look like me? Anamnesticaly -it was a love child!!! Dilemma: Does it have a right for its 50%???
    Full-text · Article · Mar 2011 · Journal of Perinatology
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    ABSTRACT: INTRODUCTION Malignant melanoma is a growing problem in pregnant women population. Incidence in literature is from 2.8-10/10000 pregnancies. OBJECTIVE Aim was to see the rate of it in our Maternity ward, during the period of 10 years, and outcome of it, toward mother and neonate. MATERIALS AND METHODS Maternity ward of Clinic Narodni front,Belgrade,had 11 cases of melanoma in last 10 years (69 023 deliveries). All diagnosed during pregnancy-none had any malignancy before. Average gestation of diagnosis-23 weeks. CLINICAL CASES OR SUMMARY RESULTS 4 delivered vaginally,7 by CS. Conservative treatment 3, conservatively + surgically 3, and surgically in 5 cases. Surgical treatments-performed in our Clinic. Chemotherapy of all in Institute Of Oncology, CCS supervised by an obstetrician. Average term of delivery - 33weeks, 4days; Apgar score 7,1/8,0; average body mass 2370 grams ; duration of hospitalization 9,1 days.1 newborn had cutaneous markings-malignant-transferred during cordocenthesis operated one day after delivery. 4 women died in 5 year period, 1 in 10 year. 6 are still in screening protocol. 3 had another child after. Surgical treatment had 8 - operations depended of localization and level of malignancy. 1 patient treated urgently after delivery - metastases inside of the eyeball-removed. CONCLUSIONS Malignancy level was high at the diagnosis. Therapy-radical and prompt-problem of saving the mother, her fertility and after that pregnancy. Incidence growth-300% in 10 years-the most vigorous problem for obstetricians, surgeons and pediatricians in time to come, concerning malignant diseases.
    No preview · Article · Jan 2010
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    ABSTRACT: INTRODUCTION Placental abruption(PA)is one of the most severe complications during pregnancy. In practice it affects 0,5% of all deliveries, our duty is to be very vigilant about it occurrence, namely for it high fatality for mother and infant. OBJECTIVE To see through delivery patients with diagnosed partial and total PA, occurring before 37 gestational weeks (GW).Also to see the conditions of the neonates after delivery, comparing the data with the control group, without PA, with delivery before 37GW. MATERIALS AND METHODS Test group-49 patients with PA. 40 of them had CS. Control group-93 patients with preterm labor, without PA. 17 had a CS. All the results were statistically tested CLINICAL CASES OR SUMMARY RESULTS PA had a much higher rate of CS (81.6:18.3%)-ratio of the duration of hospitalization was 3.6:1, for test group, cost was 6.2 times higher. Deliveries were much shorter in test group, and consumption of blood derivates was 5.3 times higher. Control-neonates had higher Apgar score (8.34:6.71).Control group lost one neonate intrapartum-test had 4. Two postpartum hysterectomies in test-control group none.Average gestation: test 34 GW, control 35 weeks 3 days. CONCLUSIONS Test-much higher rate of previous miscarriages, renal failure, hypertension, gestational diabetes and anemia. Age-no influence. Placental insertion was mainly on the front wall-test, control-evenly spread. Previous uterine operations had 13(26.7%)-test,control-7(7,52%). Even though it makes 0.5% of all deliveries PA is the highest rate killer during delivery, pre or in term-Has to be always on our minds.
    No preview · Article · Jan 2010 · Journal of Perinatology
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    ABSTRACT: INTRODUCTION Placental Abruption (PA) is one of the leading causes in perinatal morbidity and mortality. It occurs in 1:120 deliveries, makes 15% of perinatal mortality. OBJECTIVE Aim was to analyze neonatal outcome in deliveries with PA. MATERIALS AND METHODS Test group-49 neonates born in 2 year period with PA. Results were classified by gender, gestation, body mass, Apgar score and pH. Neonatal mortality/morbidity was analyzed. Control-154 neonates, born during the same period without PA. Results were statistically tested. CLINICAL CASES OR SUMMARY RESULTS Test-significantly more preterm neonates (18,7%:10,4%).Apgar score 7 and lower, test-(20.86%), control-(9.7%).Acidosis during first hour maintained in test-(35.41%), control-(11.68%). Morbidity analyze gives a high degree of perinatal asphyxia, hypoxic-ischemic encephalopathy, intracranial bleeding and anemia in test group. As a main reason for morbidity/mortality lower gestation and lower body mass were recognized in test group. CONCLUSIONS In our analysis 85.4% of neonates in test group were released as recovered or healthy. Although a small sample, it could be said that on time diagnosis, adequate way of delivery and neonatal care afterward, makes that the outcome of these deliveries could be satisfied.
    No preview · Article · Jan 2010 · Journal of Perinatology
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    ABSTRACT: Perennial ryegrass (Lolium perenne L.) is one of the most important perennial cool-season forage grasses. It is highly productive, with the highest nutritive value among forage grasses, adapted for frequent defoliation and grazing. Market demands focus interest of perennial ryegrass breeders to production of cultivars with high, stable yield and good dry matter quality, tolerant to drought, frost and other stressful environmental conditions, with different maturity. According to that, initial breeding material must be heterogeneous, with a range of different genotypes, either cultivars or wild populations. In this article the diversity in a collection of perennial ryegrass cultivars has been investigated at the beginning of the breeding process. The collection consisted of 21 genotypes which originated from Europe and USA. Within a 2-year period time of tillering, crop height in first and regeneration in second cut, annual yield and nutritive value of dry matter were investigated. The data were analysed by ANOVA on the basis of 2-year mean values and all traits showed great level of variability. Highest annual dry matter yield of about 14tha−1 was achieved by cultivars Mara and K-11, both being intermediate diploid varieties. KeywordsBreeding-Cultivar diversity-Dry matter yield-Forage quality-Perennial ryegrass
    No preview · Chapter · Dec 2009

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3 Citations
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Institutions

  • 2009-2014
    • Institut Za Krmno Bilje
      Kruševac, Central Serbia, Serbia