J.-Y. Beranger

French National Centre for Scientific Research, Lutetia Parisorum, Île-de-France, France

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Publications (6)5.62 Total impact

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    ABSTRACT: The control of human hair follicle growth and differentiation is dependent upon several well-identified factors, including androgens, cytokines, and growth factors. In humans, alopecia androgenetica is a common aging process thought to be regulated through complex genetic imbalances, which also involve several of these crucial identified factors (and probably others not yet characterized), alone or in combination. Among these factors, epidermal growth factor (EGF), as well as pro-inflammatory cytokines, play a pivotal role, as evidenced by their direct inhibitory effects on hair growth both in vitro and in vivo. Following such treatments, the in vitro growth of hair follicles was rapidly arrested and deleterious modifications of hair morphology were also observed. Because these cytokines act, at least partly, through the induction of matrix metalloproteinases (MMP), and because tissue remodeling occurs during the hair cycle, we attempted to identify and localize MMP in the human pilosebaceous unit. We used zymography to observe human hair follicles in culture in vitro. We observed that human hair follicles in culture in vitro mainly and almost exclusively produce MMP-2 and MMP-9 gelatinolytic activities. Furthermore, after stimulation with EGF, tumor necrosis factor-alpha (TNF-alpha), or interleukin-1alpha (IL-1alpha), MMP-9 production was strongly increased. Using immunohistochemistry, we then precisely localized MMP-9 in the lower part of the inner root sheath (Henle's layer) of control human anagen hair follicles. Cytokine- and EGF-induced upregulation of MMP-9 in the lower epithelial compartment of the human hair bulb is a major mechanism through which hair follicle involution, observed in alopecia, may occur.
    No preview · Article · Jul 2001 · International Journal of Dermatology
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    ABSTRACT: Human eyelid skin explants can be maintained in an appropriate culture medium in vitro for several days while retaining most of the histological features of normal skin. It thus represents a valuable tool to investigate the potentially damaging effects of biological, chemical or even physical stress that might alter their integrity. It is also a useful model to study the ability of various ingredients and products to prevent such damages and/or improve the morphological integrity of the skin. We observed here that a multi-stress inducing preparation including Substance P, Arachidonic Acid and Tumor Necrosis Factor could alter eyelid skin morphology. Dilation of superficial plexus microvasculature of the epidermis and an increase in water retention between collagen bundles of the extracellular matrix were the most reproducible histological alterations observed. We furthermore evidenced that topical application of escine and dextran sulfate synergistically protected eyelid skin explants in culture against the neuro-inflammatory stress preparation when applied prior to neuro-inflammatory stress conditions, It is thus predictable that not only these two ingredients will be well tolerated in vivo but also that they may, to some extent, protect eye skin outline against the major environmental external insults encountered day to day.
    No preview · Article · Jan 2001 · Journal of Applied Cosmetology
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    ABSTRACT: The purpose of this investigation is to examine the possible biochemical and topographic cytokeratin alterations in lichen planus of oral mucosa. Biopsy samples of clinically normal buccal mucosa (n = 5), normal gingiva (n = 5), lichen planus from buccal mucosa (n = 5), and lichen planus from gingiva (n = 5) were obtained from patients of both sexes. Cytokeratin expression was determined by means of immunohistochemical labeling with use of a battery of monoclonal antibodies against cytokeratins and filaggrin and two-dimensional gel electrophoresis. In buccal mucosa, which is not keratinized cytokeratins 4 and 13 are expressed in the majority. In buccal mucosa lichen planus, the appearance of cytokeratins 1, 2, 10, and 11 coincides with a decrease in cytokeratins 4 and 13 and a moderate increase in cytokeratins 6, 16, 17, and 19. In normal gingiva, which is normally keratinized, the main cytokeratins are 1, 2, 10, and 11. In gingival lichen planus, a slight decrease in these cytokeratins and in cytokeratin 13 expression was noted. Finally, alterations in cytokeratins 5 and 14, explained by marked alterations of basal cells, were observed. The battery of antibodies used in this study, in correlation with two-dimensional gel electrophoresis, could represent useful diagnostic tools that enable the distinction between inflammatory keratosis and so-called quiescent lichen planus. Moreover, this work showed that cytokeratins 1, 2, 10, and 11 and filaggrin are sensitive tools that may help detect early relapse before clinical exacerbation. Finally, these biochemical techniques may be useful to follow the evolution of lichen planus under treatment.
    No preview · Article · Mar 1995 · Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology
  • J Y Beranger · G Godeau · C Frances · L Robert · W Hornebeck
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    ABSTRACT: Gelatinase A and elastase type proteinase (Homsy, et al., 1988) present at plasma membranes of human skin fibroblasts (HSF) were separated by anion exchange chromatography on a DEAE Tris acryl M column. Elastase type proteinase (HSFE1) was able to convert 72 kDa progelatinase A to a lower 66 kDa M.W. active enzyme. Several cytokines (IL-1 beta, IL4, IL6), interferon gamma (IFN gamma) and tumor growth factor beta (TGF-beta) were studied for their ability to modify the levels of those plasma membrane associated proteinases. Among these mediators, only IL-1 beta was found to enhance the amounts of HSF membrane-bound HSFE1 and Gelatinase A.
    No preview · Article · Aug 1994 · Cell Biology International
  • F Prigent · C Baulac · M Duroselle · E Marinho · J Y Beranger · C Frances · C Martinet

    No preview · Article · Feb 1993 · Annales de Dermatologie et de Vénéréologie

  • No preview · Article · Jan 1993