Ping Jiang

China University of Petroleum, Tsingtao, Shandong Sheng, China

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Publications (102)218.79 Total impact

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    ABSTRACT: Porcine circovirus type 2 (PCV2) causes significant economic losses to the swine industry worldwide. Heat shock proteins (Hsps) can be used as modulators to enhance both innate and adaptive immune responses. In the present study, recombinant baculoviruses expressing the PCV2Cap protein and the N-terminal 22–370 amino acids of porcine Gp96 (Gp96N), Hsp90, and Hsp70 (rBac-cap/Gp96N, rBac-cap/Hsp90 and rBac-cap/Hsp70, respectively) were constructed and the immune responses were examined in mice and piglets. The mouse experiments showed that rBac-cap/Gp96 N increased the titers of specific anti-PCV2 neutralizing antibodies, proliferative responses of peripheral blood mononuclear cells (PBMCs) and IFN-γ levels compared to rBac-cap/Hsp90, rBac-cap/Hsp70, or rBac-cap. The pig experiments showed that the levels of anti-PCV2 antibody, proliferative responses of PBMCs, and IFN-γ in the rBac-cap/Gp96 N groups were increased compared to those in rBac-cap group. There were no clear clinical signs of infection following PCV2 challenge in pigs inoculated with recombinant rBac-cap/Gp96 N and rBac-cap, and the relative daily weight gains were higher than those in the challenge control (CC) group. The pathological lesions, extent of viremia, and viral loads of the vaccinated groups were milder than those in the CC group. Meanwhile, the extent of viremia and viral load present in the rBac-cap/Gp96 N group were significantly lower than those in the rBac-cap group. These results indicated that porcine Gp96 N effectively increased the humoral and cell-mediated immune responses of PCV2Cap. Gp96 N presents an attractive adjuvant or immunotargeting strategy to enhance the protective efficacy of PCV2 subunit vaccines in swine.
    No preview · Article · Jan 2016 · Journal of virological methods
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    ABSTRACT: Porcine reproductive and respiratory syndrome virus (PRRSV) causes one of the most economically important diseases of swine in the world. Current vaccination strategies provide only limited protection against PRRSV infection. Recently, myxovirus resistance 2 (Mx2) has been identified as a novel interferon (IFN)-induced, innate immunity restriction factor that inhibits some viral infections. However, the role of Mx2 in PRRSV infection is not well understood. In this study, we cloned the full-length monkey Mx2 (mMx2) complementary DNA (cDNA) from IFN-β-treated African green monkey Marc-145 cells, and found that overexpression of mMx2 inhibited PRRSV replication in Marc-145 cells. IFN-β induced expression of mMx2 in Marc-145 cells and suppressed PRRSV replication in a dose-dependent manner. Knockdown of mMx2 impaired the antiviral activity mediated by IFN-β. Confocal imaging and immunoprecipitation assays indicated that mMx2 interacted with PRRSV N protein in virus-infected cells. Furthermore, we showed that GTPase activity of mMx2 is necessary, but that the first N-terminal 51 amino acids are dispensable for antiviral activity. Finally, porcine Mx2 was also found to have the antiviral activity against PRRSV in Marc-145 cells. We conclude that mMx2 protein inhibits PRRSV replication by interaction with the viral N protein.
    No preview · Article · Nov 2015 · Journal of Interferon & Cytokine Research
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    ABSTRACT: Acrylamide copolymer hydrogels used in profile modification applications in an oilfield share a common problem, termed syneresis, which decreases the efficiency of profile modification. In this paper, sodium tripolyphosphate (STPP) was found to be an effective syneresis inhibitor for the hydrogel formulated with acrylamide copolymer of acryloyloxyethyl trimethylammonium chloride (AM/DAC), and an ultrastable hydrogel for enhanced oil recovery in high-temperature and high-salinity petroleum reservoirs was obtained on the basis of double-groups cross-linking. Experimental investigations, including DLS, FTIR, NMR, SEM, and core flood test, have been conducted to elucidate the mechanism of STPP inhibiting the hydrogel syneresis in the aspect of the reaction between STPP and AM/DAC. The result showed that the AM/DAC cross-links with STPP on the basis of the hydrolysis reaction of the ester group and STPP, whereby the new bond of C-O-P is formed. For this reason, the viscosity and hydrophilicity of AM/DAC were significantly increased by STPP, and the generated double-groups cross-linking (AM/DAC cross-linked with phenol-formaldehyde, AM/DAC cross-linked with STPP) made more AM/DAC molecule chains cross-linked together; therefore, the stronger grid structure was formed. Therefore, the increase of the hydrophilicity and the improved stability of grid structure enhanced the water-holding capacity of the hydrogel, leading to the decrease of the hydrogel syneresis and the increase of the water-shutoff efficiency.
    No preview · Article · Oct 2015 · Energy & Fuels
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    ABSTRACT: Experimental investigations have been conducted to elucidate the mechanism of sodium tripolyphosphate (STPP) inhibiting hydrogel syneresis with respect to the reaction between STPP and partly hydrolyzed polyacrylamide (HPAM). Viscosity measurements indicate that STPP can increase the viscosity of HPAM. The results of light scattering measurements show that STPP can improve the hydrophilicity of HPAM since both hydrodynamic radius (Rh) and second virial coefficient (A2) of HPAM are increased by STPP, and STPP can also increase the weight average molecular weight (Mw) of HPAM. FTIR and NMR measurements show that the mechanism of the above effect of STPP on HPAM is the following: STPP molecule can react with the C-O in the -COO- group of HPAM whereby the new bond of C-O-P is formed, so a large quantity of STPP molecules "adsorb" on the molecular chains of HPAM and produce the intramolecular or intermolecular crosslinking between HAPM and STPP. As a result, the increase of the hydrophilicity and stability of the crosslinked HPAM improve the water-holding capacity of the hydrogel, and then the hydrogel syneresis is decreased. On the basis of the crosslinking reaction between STPP and HPAM, a hydrogel, which can be successfully used at 160 °C, has been developed with HPAM and Cr3+ for the first time. Therefore, STPP is an effective syneresis inhibitor for the hydrogel formulated with HPAM, and it deserves to be popularization and applied in enhancing hydrogel stability.
    No preview · Article · Oct 2015 · RSC Advances
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    Baochao Fan · Xing Liu · Juan Bai · Yufeng Li · Qiaoya Zhang · Ping Jiang
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    ABSTRACT: Porcine reproductive and respiratory syndrome virus (PRRSV) negatively modulates host immune responses, resulting in persistent infection and immunosuppression. PRRSV infection increases the number of PRRSV-specific regulatory T lymphocytes (Tregs) in infected pigs. However, the target antigens for Tregs proliferation in PRRSV infection have not been fully understood. In this study, we demonstrated that the highly pathogenic PRRSV (HP-PRRSV) induced more CD4+CD25+Foxp3+ Tregs than classical PRRSV (C-PRRSV) strain. Of the recombinant GP5, M and N proteins of HP-PRRSV expressed in baculovirus expression systems, only N protein induced Tregs proliferation. The Tregs assays showed that three amino-acid regions, 15-21, 42-48 and 88-94, in N protein played an important role in induction of Tregs proliferation with synthetic peptides covering the whole length of N protein. By using reverse genetic methods, it was firstly found that the 15N and 46R residues in PRRSV N protein were critical for induction of Tregs proliferation. The phenotype of induced Tregs closely resembled that of transforming-growth-factor-β-secreting T helper 3 Tregs in swine. These data should be useful for understanding the mechanism of immunity to PRRSV and development of infection control strategies in the future.
    Preview · Article · Sep 2015 · PLoS ONE
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    ABSTRACT: Porcine epidemic diarrhea (PED) is a highly contagious, enteric disease of swine caused by the porcine epidemic diarrhea virus (PEDV). To find a suitable ELISA method to assess the infection of PEDV and the effectiveness of vaccines, we developed and evaluated an indirect enzyme-linked immunosorbent assay (iELISA) based on a truncated recombinant spike (S) protein expressed in Escherichia coli. The parameters of the iELISA were optimized, and the cutoff value determined as 0.259 by analyzing optical density (OD) values of 80 PEDV negative sera confirmed by western blot. Repeatability tests revealed that the coefficients of variation of positive sera within and between runs were both less than 10%. Cross-reactivity assays demonstrated that iELISA was PEDV-specific. A virus neutralization test with sera of 7 different OD values showed a positive correlation between the OD values and virus neutralization. The results suggest this iELISA is specific, sensitive, and repeatable. Further studies should focus on the relationship between OD values of sera and its virus neutralization.
    No preview · Article · Aug 2015 · Canadian Journal of Microbiology
  • Yang Wang · Jijiang Ge · Yufei Zheng · Ping Jiang · Guicai Zhang
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    ABSTRACT: In order to find out the influence of dilational rheology on chemical flooding systems, the dilational rheology of Zhuangxi crude oil/NaOH solutions interface was investigated by DSA100 interfacial dilatational rheometer (KRÜSS, Germany). The results show that the alkaline system, which can enhance oil recovery, mainly depends on its interfacial dilational rheology, rather than interfacial tension. The greater the interfacial dilational elasticity, the higher the oil recovery is. The greater the interfacial dilational viscosity, the lower the oil recovery is. The conclusion is consistent with the change of displacement pressure in the macro displacement experiment and the change of sweep efficiency in the microscopic displacement experiment. © Carl Hanser Publisher, Munich.
    No preview · Article · Jul 2015 · Tenside Surfactants Detergents
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    ABSTRACT: Experimental investigations have been conducted to elucidate the syneresis mechanism of HPAM (partly hydrolyzed polyacrylamide)/Cr3+ gel. The gel prepared with HPAM of high hydrolysis degree is apt to result in the syneresis, since HPAM with more carboxylate group is easier to be over-crosslinked. The over-crosslinking, which contributes a lot to the syneresis, occurs between CO bond in COO- of HPAM and Cr3+. The decreasing hydrophilicity of HPAM molecule, the increase of crosslinking density, and the complexation of the carboxylate group with Ca2+ are the main syneresis mechanisms of the gel influenced by the inorganic salt. The Ca2+ reacts with the CO bond in COO-, and the reaction produces a tabular structure when the concentration of Ca2+ is high, whereby the water in the initial gel is extruded. The produced water resulted from the syneresis mainly originates from the water bounded to the carboxylate group of HPAM, and this confirms that the decreasing hydrophilicity of the carboxylate group in HPAM molecule is one of the important reasons to the gel syneresis. Sodium d-isoascorbate inhibits the generation and growth of the polynuclear olation complex by coordinating with Cr3+, whereby the crosslinking speed is decreased and the syneresis is suppressed.
    No preview · Article · Jul 2015 · Colloids and Surfaces A Physicochemical and Engineering Aspects
  • Yang Wang · Jijiang Ge · Guicai Zhang · Ping Jiang · Wen Zhang · Yang Lin
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    ABSTRACT: Herein is presented a new methodology to determine the static adsorption of dodecyl hydroxypropyl sulfo betaine (DSB) on limestone with the use of HPLC. The results showed that with the increase of NaCl concentration, adsorption of DSB on limestone surface decreased due to increase of zeta potential (less negative). In contrast, with increasing CaCl2 concentration, adsorption of DSB decreased first and then increased, which corresponds to the change trend of limestone surface zeta potential. As to the influence of temperature, with increase of temperature, adsorption of DSB increased slightly. The behavior of DSB adsorption under different temperatures could be well interpreted by more adsorption of Ca2+ at higher temperature. It was also found that a little addition of inorganic salt could accelerate formation of vesicles and more salt would inhibit the formation. By comparison of aggregation distribution before and after adsorption, it was found that micelles contribute more to adsorption than vesicles.
    No preview · Article · Jul 2015 · RSC Advances
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    ABSTRACT: Pseudorabies has been controlled efficiently in China for many years by vaccination. However, it suddenly broke out in many pig farms in 2012-2013 in southern China. In this study, a systematic investigation that included virus isolation, genetic and pathological studies, and immunogenicity analysis was carried out with the aim of understanding the pathogenetic and antigenic features of novel isolates of pseudorabies virus (PRV). Of 38 tissue samples collected from pigs with clinical signs of pseudorabies on 13 farms in 4 provinces in southern China in 2012-2013, 29 showed wild-type PRV infection by polymerase chain reaction. Sequence analysis of 5 isolates from the 4 provinces showed that they belonged to a relatively independent cluster that shared 2 insertions of a single amino acid in the gE gene and 1 insertion of 7 amino acids in the gC gene. In experiments, isolate ZJ01 caused death in 100% of pigs that were either 14 or 80 days old. The serum antibodies to the commercial PRV vaccines had significantly lower neutralizing activity against the ZJ01 isolate than against the vaccine strains. The antigenic relatedness between ZJ01 and the vaccine strains was 0.378 to 0.455. These findings indicated that a novel, highly virulent PRV strain with antigenic variance had spread widely in southern China.
    No preview · Article · Jul 2015 · Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire
  • Xing Liu · Juan Bai · Haiyan Wang · Baochao Fan · Yufeng Li · Ping Jiang
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    ABSTRACT: Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen that is responsible for large economic losses in the swine industry worldwide. In PRRSV strains, many genetic variations occur in the central hypervariable region (HV2) of the Nsp2 gene, which encodes non-structural protein 2. For example, PRRSV strains VR2332, Em2007, MN184C, and TJM-F92 contained variations in the Nsp2 sequences and exhibited differing levels of virulence in adult pigs. However, the role of HV2 with respect to PRRSV immunity is unclear. In this study, four recombinant PRRSV strains (rBB/+30aa, rBB/Δ68aa, rBB/Δ111aa, and rBB/Δ120aa) were rescued using a highly pathogenic type 2 PRRSV cDNA clone (pBB). All rescued strains displayed similar growth characteristics to the parental rBB virus in pulmonary alveolar macrophages (PAMs). Expression levels of inflammatory cytokines IL-β, IL-6, and TNF-α were significantly lower, at the mRNA and protein level, for groups infected with rBB/Δ111aa and rBB/Δ120aa than those in the rBB group. Levels of these inflammatory cytokines in the rBB/+30aa and rBB/Δ68aa groups were not significantly different with those in the rBB group. Phosphorylation levels of IκB were decreased to a greater extent in the rBB/Δ111aa and rBB/Δ120aa groups compared with those in the rBB/+30aa, rBB/Δ68aa, and rBB groups. Our results indicate that amino acids 323-433 and 628-747 of Nsp2 failed to exert significant effects on PRRSV replication in PAMs, but modulated the expression of inflammatory cytokines in vitro. Copyright © 2015. Published by Elsevier B.V.
    No preview · Article · Jun 2015 · Virus Research
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    ABSTRACT: Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) are very two important pathogens that have coursed huge economic losses in swine production in worldwide. In this study,a vector pCMV-TJM containing the full-length cDNA clone of PRRSV attenuated strain TJM-F92 was firstly constructed by PCR method. Then a gene sequence containing Afl II/Mlu I e restriction enzyme sites and a transcription regulatory sequence for ORF6 (TRS6) was inserted be- tween ORF7 and 3'UTR, yielding a expression vector pCMV-TJM-TRS. Subsequently, a plasmid pCMV-TJM-Cap was constructed by cloning of PCV2 ORF2 gene into the unique sites Afl II /Mlu I of pCMV- TJM-TRS plasmid DNA. Then three recombinant PRRSV, rTJM, rTJM/TRS and rTJM/Cap, were rescued by transfection of pCMV-TJM, pCMV-TJM-TRS and pCMV-TJM-Cap into Marc-145 cells, respectively,and confirmed by the genome sequence, restriction enzyme digestion, Western Blot and IFA. They all had the molecular markers which was different from the parent virus. The growth characteristics of the rescued viruses were similar to that of parent virus. rTJM/Cap could also express efficiently PCV2 Cap protein in Marc-145 cells. At passage 8, it still had PCV2 ORF2 gene which examined by RT-PCR. It indicated that the full-length cDNA clone of PRRSV attenuated strain TJM-F92 and recombinant PRRSV rTJM/Cap expressing PCV2 Cap protein were successfully constructed. It made an important foundation for studying on the pathogenic mechanisms of PRRSV and PRRSV-PCV2 vaccine in the future.
    No preview · Article · May 2015 · Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui]
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    ABSTRACT: Secretory component (SC) is a component of secretory IgA that is designated sIgA to distinguish it from IgA. The monoclonal antibody (MAb) against SC has been shown to be an excellent tool for the detection of the level of sIgA and for the evaluation of the efficacy of mucosal immunity. To prepare a monoclonal antibody against porcine SC, a recombinant porcine SC was expressed and purified. To develop this recombinant SC, the gene encoding the porcine SC was ligated into the pCold I vector. The recombinant vector was then transformed into Escherichia coli BL 21 (DE3), and gene expression was successfully induced by isopropyl-β-d-thiogalactoside (IPTG). After affinity purification with Ni-NTA resin and gel recovery, the recombinant SC protein was used to immunize BALB/c mice. Finally, three hybridoma cell lines showing specific recognitions of both recombinant SC and native SC were used as stable secretors of MAbs against porcine SC and were confirmed to have no reaction to porcine IgA or IgG. The successful preparation of recombinant SC protein and MAbs provides valuable materials that can be used in the mucosal infection diagnosis for porcine disease and mucosal immune evaluation for porcine vaccine, respectively. Copyright © 2015. Published by Elsevier Inc.
    No preview · Article · May 2015 · Protein Expression and Purification
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    ABSTRACT: Porcine reproductive and respiratory syndrome virus (PRRSV) is mainly responsible for the heavy economic losses in pig industry in the world. A number of neutralizing epitopes have been identified in the viral structural proteins GP3, GP4, GP5 and M. In this study, the important amino acid (aa) residues of HP-PRRSV strain BB affecting neutralization susceptibility of antibody were examined using resistant strains generated under neutralizing antibody (NAb) pressure in MARC-145 cells, reverse genetic technique and virus neutralization assay. HP-PRRSV strain BB was passaged under the pressure of porcine NAb serum in vitro. A resistant strain BB34s with 102 and 104 aa substitutions in GP5, which have been predicted to be the positive sites for pressure selection (Delisle et al., 2012), was cloned and identified. To determine the effect of the two aa residues on neutralization, 8 recombinant PRRSV strains were generated, and neutralization assay results confirmed that the aa residues 102 and 104 in GP5 played an important role in NAbs against HP-PRRSV in MARC-145 cells and porcine alveolar macrophages. Alignment of GP5 sequences revealed that the variant aa residues at 102 and 104 were frequent among type 2 PRRSV strains. It may be helpful for understanding the mechanism regulating the neutralization susceptibility of PRRSV to the NAbs and monitoring the antigen variant strains in the field. Copyright © 2015. Published by Elsevier B.V.
    No preview · Article · Apr 2015 · Virus Research
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    ABSTRACT: Protease-activated receptor 4 (PAR4), a member of G-protein coupled receptors family, was recently reported to exhibit decreased expression in gastric cancer and esophageal squamous cancer, yet increased expression during the progression of prostate cancer. Trefoil factor 2 (TFF2), a small peptide constitutively expressed in the gastric mucosa, plays a protective role in restitution of gastric mucosa. Altered TFF2 expression was also related to the development of gastrointestinal cancer. TFF2 has been verified to promote cell migration via PAR4, but the roles of PAR4 and TFF2 in the progress of colorectal cancer are still unknown. In this study, the expression level of PAR4 and TFF2 in colorectal cancer tissues was measured using real-time PCR (n = 38), western blotting (n=38) and tissue microarrays (n = 66). The mRNA and protein expression levels of PAR4 and TFF2 were remarkably increased in colorectal cancer compared with matched noncancerous tissues, especially in positive lymph node and poorly differentiated cancers. The colorectal carcinoma cell LoVo showed an increased response to TFF2 as assessed by cell invasion upon PAR4 expression. However, after intervention of PAR4 expression, PAR4 positive colorectal carcinoma cell HT-29 was less responsive to TFF2 in cell invasion. Genomic bisulfite sequencing showed the hypomethylation of PAR4 promoter in colorectal cancer tissues and the hypermethylation in the normal mucosa that suggested the low methylation of promoter was correlated to the increased PAR4 expression. Taken together, the results demonstrated that the up-regulated expression of PAR4 and TFF2 frequently occurs in colorectal cancer tissues, and that overexpression of PAR4 may be resulted from promoter hypomethylation. While TFF2 promotes invasion activity of LoVo cells overexpressing PAR4, and this effect was significantly decreased when PAR4 was knockdowned in HT-29 cells. Our findings will be helpful in further investigations into the functions and molecular mechanisms of Proteinase-activated receptors (PARs) and Trefoil factor factors (TFFs) during the progression of colorectal cancer.
    Full-text · Article · Apr 2015 · PLoS ONE
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    ABSTRACT: Porcine diarrhea outbreaks caused by porcine epidemic diarrhea virus (PEDV) has occurred in China with significant losses of piglets since 2010. In this study, the complete S and ORF3 genes of 15 field PEDV isolates in mid-eastern China from 2011 to 2013 were detected and compared with other reference strains. Based on S gene, all of the PEDV strains could be assigned to 3 genogroups. Only 1 isolate, JS120103, belonged to genogroup 1 and showed a close relationship with previous Chinese strains DX and JS-2004-2, European strain CV777, and Korean strain DR13. The other 14 isolates belonged to genogroup 3 and showed a close relationship with other Chinese strains isolated after 2010. The S genes of those isolates were 9 nucleotides longer in length than JS120103 and the other reference strains in genogroup 1, with 15 bp insertion and 6 bp deletion. Homology analyses revealed that all of the Chinese field isolates, except JS120103, are 97.6% to 100% (95.8% to 100%) identical in nucleotide (deduced amino acid) sequence to each other. Meanwhile, based on the ORF3 gene, all of the PEDV isolates could be separated into 3 genogroups. Eleven of the 15 field isolates in this study belonged to genogroup 3 and were 95.8% to 100% identical in nucleotide sequence or 95.6% to 100% in deduced amino acid sequence to each other. Our results indicate that the variant PEDV strain spread wildly in mid-eastern China. This will be useful to take into consideration in the control and prevention of this disease.
    No preview · Article · Feb 2015 · Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire
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    ABSTRACT: Recently, a series of acute swine erysipelas outbreaks occurred in Eastern China. Eight strains isolated from cases of septicemia were determined as serotype 1a, and 4 of the isolates were resistant to acriflavine. One isolate strain named HX130709 was attenuated on agar media containing acriflavine dye. The 432-bp hypervariable region in spaA gene of the field and attenuated strains were amplified and sequenced. It was further compared with the vaccine strain G4T10, and thus, the eight field strains can be divided into four spaA-types. The partial spaA gene analysis also showed that no point mutations occurred among different archived passages of HX130709 during the attenuation. Results of pulsed-field gel electrophoresis showed that eight distinct patterns with 22 to 30 DNA fragment bands were produced from field strains, and twelve distinct patterns with 23 to 27 DNA fragment bands were produced from different passages of the attenuated strains. Mouse pathogenicity test showed that the mortality of the mice infected with 104 CFU field strains was 100% and the attenuation of strain HX130709 occurred between 46 and 50 passages. All the field and attenuated strains were highly sensitive to β-lactam antibiotics, tetracyclines and macrolides. So, we can make conclusions that the acute swine erysipelas outbreaks in Eastern China were caused by serotype 1a E. rhusiopathiae strains with different biochemical characteristics, and the virulence of serotype 1a E. rhusiopathiae strains is unrelated with some point mutations in 432-bp hypervariable region of the spaA gene.
    Full-text · Article · Feb 2015 · Journal of Veterinary Medical Science
  • Xing Liu · Baochao Fan · Juan Bai · Haiyan Wang · Yufeng Li · Ping Jiang
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    ABSTRACT: Porcine reproductive and respiratory syndrome virus (PRRSV) usually establishes a prolonged infection and causes an immunosuppressive state. It has been proposed that interleukin-10 (IL-10) plays an important role in PRRSV-induced immunosuppression. However, this mechanism has not been completely elucidated. In this study, we found that transfection of 3D4/2 macrophages with the N protein gene of type 2 PRRSV significantly upregulated IL-10 expression at the transcriptional level. Moreover, alanine substitution mutation analysis revealed that the N protein residues 33-37, 65-68, and 112-123 were related to the upregulation of IL-10 promoter activity. Recombinant PRRSV with mutations at residues 33-37 in the N protein (rQ33-5A and rS36A), recovered from corresponding infectious cDNA clones, induced significantly lower levels of IL-10 production in infected monocyte-derived dendritic cells, as compared to their revertants rQ33-5A(R) and rS36A(R), and the wild-type recombinant PRRSV strain rNT/wt. These data indicate that type 2 PRRSV N protein plays an important role in IL-10 induction and the N-N non-covalent domain is associated with this activity.
    No preview · Article · Jan 2015 · Journal of General Virology
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    ABSTRACT: Background Encephalomyocarditis virus (EMCV) can cause myocarditis, respiratory failure, reproductive failure, and sudden death in pre-weaned piglets, which has been isolated in China. EMCV VP1 protein was one of the most important structural proteins and played an important role in the protective immunity. In this study, 10 monoclonal antibodies (McAbs) against EMCV VP1 were screened and identified.ResultsEpitope mapping results indicated that McAbs (6E11, 7A7, 7C9) specifically recognized the linear epitopes V(2)ENAEK(7), McAbs (1D1, 2A2, 5A1, 5A11, 5G1) recognized the epitope F(19)VAQPVY(25), and McAbs 1G8 and 3A9 recognized P(42)IGAFTVK(49). Protein sequence alignment of VP1 with 16 EMCV isolates indicated that the epitope F(19)VAQPVY(25) was conserved in all the reference strains. The epitopes P(42)IGAFTVK(49) and V(2)ENAEK(7) only had 1 or 2 variable amino acid among the reference strains. The 3D model analysis results showed that these epitopes presented as spheres were shown within the context of the complete particle.Conclusions In this study, ten McAbs against EMCV VP1 were developed and three B-cells epitopes (2-7aa, 19-25aa and 42-49aa) were defined in VP1. All the results herein will promote the future investigations into the function of VP1 of EMCV and development of diagnostic methods of EMCV.
    Preview · Article · Dec 2014 · Virology Journal
  • Puxian Fang · Juan Bai · Xing Liu · Jing Dong · Tao Sun · Ping Jiang
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    ABSTRACT: Encephalomyocarditis virus (EMCV) infects animals of various species and causes a variety of clinical symptoms. In this study, an infectious full-length cDNA clone was constructed, and the characteristics of the rescued virus were investigated in vitro and in vivo. Our data demonstrated that the growth kinetics in vitro and plaque morphology of the rescued EMCV rNJ08 strain were similar to those of the parental strain. Although rNJ08 infected BALB/c mice, none of the mice died during the observation period of 14 days post-inoculation. The availability of the infectious cDNA clone provides a genetic platform for studying gene function and for the rational design of vaccines.
    No preview · Article · Nov 2014 · Archives of Virology

Publication Stats

1k Citations
218.79 Total Impact Points


  • 2012-2015
    • China University of Petroleum
      Tsingtao, Shandong Sheng, China
  • 2010-2015
    • Kunming Institute of Zoology CAS
      • State Key Laboratory of Genetic Resources and Evolution
      Yün-nan, Yunnan, China
  • 2007-2015
    • Nanjing Agricultural University
      • College of Life Sciences
      Nan-ching, Jiangsu Sheng, China
  • 2012-2014
    • China University of Petroleum
      Ch’ang-p’ing-ch’ü, Beijing, China
  • 2011
    • Kunming Medical College
      • Department of Pathology
      Yün-nan, Yunnan, China