Peter Schumann

Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Brunswyck, Lower Saxony, Germany

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Publications (444)1070.84 Total impact


  • No preview · Article · Apr 2016 · International Biodeterioration & Biodegradation
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    ABSTRACT: During a screening for novel and biotechnologically useful bacteria in haloalkaline lakes, strain No.156T was isolated from a sediment sample from lake Elmenteita in the African Rift Valley and studied by a polyphasic taxonomic approach. The strain was observed to form yellow aerial and substrate mycelia; optimal growth was found to be at 30–35 °C in salt concentrations of 6–9 % (w/v) and at pH 7–9. The DNA G+C content of the novel strain was 71 mol%. Analysis of 16S rRNA sequences indicated that the isolate belongs to the genus Nocardiopsis with sequence similarities below 98 % to the type strains of all other representatives of the genus. Mycolic acids were not detected in whole cell methanolysates. The peptidoglycan was found to contain meso-diaminopimelic acid as the diamino acid with no diagnostic sugars. The main polar lipids were identified as phosphatidylmethylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol but no diphosphatidylglycerol. The predominant menaquinones were MK-11(H8), MK-11(H6), MK-10(H8) and MK-10(H6). Cellular fatty acids were found to consist of saturated and monounsaturated iso- and anteiso-branched acids with 16–18 C-length, tuberculostearic acid (Me18:0), and straight-chain saturated (16:0, 18:0) acids. These characteristics match those of the genus Nocardiopsis. Based on 16S rRNA gene sequence analysis and phenotypic characteristics, a novel species with the name Nocardiopsis mwathae is proposed. The type strain is No.156T (=DSM 46659T = CECT 8552T). The INSDC accession number for the 16S rRNA gene sequence of strain No.156T is KF976731.
    No preview · Article · Jan 2016 · Antonie van Leeuwenhoek
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    ABSTRACT: A novel actinobacterium, designated strain SG20T, was isolated from a Saharan soil sample collected from Béni-isguen (Mzab), Ghardaïa province (South Algeria). The microorganism developed on aerial mycelium, small roundish sporangia, sessile or carried by very short sporangiophores. The cell-wall peptidoglycan of the novel actinobacterium contained meso-diaminopimelic acid and the whole-cell sugars comprised glucose, ribose and mannose, but madurose was not detected. The predominant menaquinones were MK-9(H4), MK-9(H6) and MK-9(H2). The major fatty acids were iso-C16 : 0 and C16 : 0. The phospholipids detected were diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine and unknown lipids. The phenotypic and chemotaxonomic characteristics of the novel strain resembled those of established members of the genus Streptosporangium. Moreover, phylogenetic analysis based on a 16S rRNA gene sequence generated from the strain identified its closest relative as Streptosporangium jomthongense DSM 46822T (98.5 % sequence similarity), which produces single spores on aerial mycelium, but no sporangia. In hybridization experiments, the DNA-DNA relatedness values recorded between the strain SG20T and its nearest relative species S. jomthongense DSM 46822T fell well below 70 %. On the basis of phenotypic and genotypic data, strain SG20T can be distinguished as a novel species of the genus Streptosporangium, for which the name Streptosporangium saharense sp. nov., is proposed. The type strain is SG20T (=DSM 46743T=CECT 8840T).
    No preview · Article · Jan 2016 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: A novel thermophilic filamentous bacterium, designated strain T36T, was isolated from soil sediment sample from a hot spring source collected in Khenchela province, Algeria. Strain T36T was identified as a member of the genus Thermoactinomyces by a polyphasic approach. Strain T36T was observed to form white aerial mycelium and non-coloured to pale yellow substrate mycelium, both producing endospores, sessile or borne by short sporophores. The optimum growth temperature and pH were found to be 37–55 °C and 7.0–9.0, respectively and the optimum NaCl concentration for growth was found to be 0–7 % (w/v). The diagnostic diamino acid in the cell wall peptidoglycan was identified as meso-diaminopimelic acid. The predominant menaquinone of strain T36T was identified as MK-7 (H0). The major fatty acids were found to be iso-C15:0 and iso-C17:0. The phospholipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphoglycolipid. The chemotaxonomic properties of strain T36T are consistent with those shared by members of the genus Thermoactinomyces. 16S rRNA gene sequence analysis indicated that the sequence similarities between strain T36T and Thermoactinomyces species with validly published names were less than 98 %. Based on the combined genotypic and phenotypic evidence, it is proposed that strain T36T should be classified as representative of a novel species, for which the name Thermoactinomyces khenchelensis sp. nov. is proposed. The type strain is T36T (=DSM 45951T = CECT 8579T).
    No preview · Article · Dec 2015 · Antonie van Leeuwenhoek
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    ABSTRACT: The Gram-stain-variable, rod-shaped, motile bacterial strain, designated S1-20T, was isolated from marine sediment. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain S1-20T was related to the genus Virgibacillus and had highest 16S rRNA gene sequence similarities with the type strains of Virgibacillus necropolis JCM 16509T(98.1 %), Virgibacillus arcticus JCM 14839T (97.7 %) and Virgibacillus carmonensis JCM 16508T (97.3 %). The predominant cellular fatty acids were anteiso-C15:0 and anteiso-C17:0. The main menaquinone was MK-7. The genomic DNA G+C content of strain S1-20T was 38.3 mol%. The polar lipids profile contained diphosphatidylglycerol, phosphatidylglycerol, five unidentified phospholipids, one unknown aminophospholipid and an aminolipid. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that the strain S1-20T is a representative of a novel species of the genus Virgibacillus, for which we propose the name Virgibacillus flavescens sp. nov. (type strain S1-20T = LMG 28381T = DSM 29015T).
    No preview · Article · Dec 2015 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: The taxonomic position of a novel actinobacterium, strain 169T, isolated from a sample of Algerian Saharan soil, was determined using a polyphasic taxonomic approach. The aerial mycelium produces non-motile, round to oval shaped spores, with a smooth surface, sessile or carried by short sporophores. Chemotaxonomically, the isolate 169T showed the same results as members of the genus Streptosporangium, but madurose, the so far diagnostic sugar of the genus, was not detected in the whole-cell hydrolysate. Despite the absence of sporangia, the 16S rRNA gene sequence analysis confirmed that strain 169T was a member of the genus Streptosporangium. Strain 169T was most closely related to Streptosporangium jomthongense DSM 46822T (99.3 %), which is the only non-sporangia-forming species reported among the genus. However, DNA-DNA hybridization studies with this species showed 60 % similarity. Based upon genotypic and phenotypic data, a novel species, Streptosporangium algeriense sp. nov., is proposed, with 169T (=DSM 45455T =MTCC 11561T =CCUG 62974T) as the type strain.
    No preview · Article · Dec 2015 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: A novel orange pigmented halophilic archaeon, strain DC8T, was isolated from Urmia salt lake in North-West Iran. The cells of strain DC8T were non-motile and pleomorphic, from small rods to triangular or disk shaped. The novel strain needs at least 2.5 M NaCl and 0.02 M MgCl2 for growth. Optimal growth was achieved at 4.0 M NaCl and 0.1 M MgCl2. The optimum pH and temperature for growth were pH 7.5 and 45 °C, respectively, and it was able to grow over a pH range of 7.0 to 8.5 and a temperature range of 25 to 55 °C. Analysis of the 16S rRNA gene sequence showed that strain DC8T was a member of the family Halobacteriaceae; however, its similarity was as low as 90.1 %, 89.3 % and 89.1 % to the most closely related haloarchaeal taxa including Halosimplex, Halobaculum and Halomicrobium type species, respectively. The G+C content of its DNA was 68.1 mol %. Polar lipid analyses revealed that strain DC8T contains phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulphate and phosphatidic acid. One unknown phospholipid, two major glycolipids and one minor glycolipid were also observed. The only quinone presented was MK-8 (ΙΙ-H2). The physiological, biochemical and phylogenetic differences between strain DC8T and other previously described extremely halophilic archaeal genera supported that this strain represents a novel species of a new genus within the family Halobacteriaceae, for which the name Halosiccatus urmianus gen. nov., sp. nov. is proposed. The type strain is strain DC8T (= IBRC-M 10911T= CECT 8793T).
    No preview · Article · Nov 2015 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: During the course of a screening programme for new taxa of actinobacteria, a strain designated ACD1T, was isolated from a Saharan soil in the Hoggar region (Algeria). The taxonomic position of this strain was determined using a polyphasic taxonomic approach. The strain was observed to form extensively branched, non-fragmenting substrate mycelium, and aerial mycelium with straight to flexuous, hooked and irregular spirals (1–2 turns) forming short chains of spores. The diamino acid present in the cell wall is meso-diaminopimelic acid. Galactose, glucose, madurose, mannose and ribose occur in whole-cell hydrolysates. The diagnostic phospholipids detected were diphosphatidylglycerol and phosphatidylinositol. The major menaquinones were identified as MK-9 (H4) and MK-9 (H2). The major fatty acids were found to be C16:0, C18:1cis9, iso-C16:0 and 10-methyl C18:0. Phylogenetic analysis based on the 16S rRNA gene showed that the strain belongs to the genus Actinomadura, and is closely related to Actinomadura sediminis DSM 45500T (98.5 % similarity) and Actinomadura cremea subsp. cremea DSM 43676T (98.3 % similarity). However, DNA–DNA hybridization revealed only 48.0 % relatedness with A. sediminis DSM 45500T and 33.2 % relatedness with A. cremea subsp. cremea DSM 43676T. The combined phenotypic and genotypic data showed that the strain represents a novel species of the genus Actinomadura, for which the name Actinomadura algeriensis sp. nov. is proposed, with the type strain ACD1T (= DSM 46744T = CECT 8841T).
    No preview · Article · Nov 2015 · Antonie van Leeuwenhoek
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    ABSTRACT: A Gram-positive, rod-shaped, endospore-forming, aerobic bacterium, isolate FJAT-4402T was isolated from the weed rhizosphere soil of gobi desert in Xinjiang of China. On the basis of the polyphasic taxonomic data, the isolate was considered to be a member of the genus Bacillus. Isolate FJAT-4402T grew at 15-40 °C (optimum 30 °C), pH 5-10 (optimum pH 7) and 0-3% (w/v) NaCl (optimum 0%). Diagnostic diamino acid of the peptidoglycan of isolate FJAT-4402T was meso-diaminopimelic acid and the predominant isoprenoid quinone was MK-7. The major cellular fatty acids were iso-C15:0 (20.1%), anteiso-C15:0, iso-C14:0, C16:0, iso-C16:0, iso-C17:0 and anteiso-C17:0. The polar lipids detected were diphosphatidylglycerol, phosphatidylglycerol, unknown glycolipid and one unknown phospholipids. The G+C content of the genomic DNA was 42.0 mol%. Isolate FJAT-4402T exhibited 16S rRNA gene sequence similarities of 96.2% and 96.15% with its nearest neighbors B. licheniformis DSM 13T and B. sonorensis DSM 13779T, respectively. The isolate showed 33.3% DNA-DNA relatedness to the closest reference isolate B. licheniformis DSM 13T. Phenotypic, chemotaxonomic and genotypic properties clearly indicated that isolate FJAT-4402T represents a novel species within the genus Bacillus, for which the name Bacillus gobiensis sp. nov. ( type strain, FJAT-4402T= DSM 29500T = CGMCC 1.12902T) is proposed.
    No preview · Article · Nov 2015 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: In the context of studying the influence of N-fertilization on N2 and N2O flux rates in relation to the soil bacterial community composition in fen peat grassland, a group of bacterial strains was isolated that performed dissimilatory nitrate reduction to ammonium and concomitantly produced N2O. The amount of nitrous oxide produced was influenced by the C/N ratio of the medium. The potential to generate nitrous oxide was increased by higher availability of nitrate-N. Phylogenetic analysis based on the 16S rRNA and the rpoB gene sequences demonstrated that the investigated isolates belong to the genus Proteus, showing high similarity with the respective type strains of Proteus vulgaris and Proteus penneri. DNA-DNA hybridization studies revealed differences at the species level. These differences were substantiated by MALDI-TOF MS analysis and several distinct physiological characteristics. On the basis of these results, it was concluded that the soil isolates represent a novel species for which the name Proteus terrae sp. nov. (type strain N5/687(T) =DSM 29910(T) =LMG 28659(T)) is proposed.
    No preview · Article · Oct 2015 · Antonie van Leeuwenhoek
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    ABSTRACT: In 2008, three clinical isolates (W9405(T), W9409 and W9575) were obtained from bronchial wash or sputum specimens from patients from the state of Arizona and characterised by polyphasic analysis. All three clinical isolates 16S rRNA gene sequences were found to be 100 % identical to each other and showed the strains belong in the genus Nocardia. BLASTn searches in the GenBank database of near full-length 16S rRNA gene sequences showed the highest sequence similarities to the type strains of Nocardia takedensis (98.3 %, sequence similarity), Nocardia lijiangensis (97.4 %), Nocardia harenae (97.4 %), and Nocardia xishanensis (97.1 %). The DNA-DNA relatedness between isolate W9405(T) and the type strain of N. takedensis is 26.0 ± 2.4 % when measured in silico using genomic DNA sequences. The G+C content of isolate W9405(T) is 68.6 mol%. Chemotaxonomic analyses of the clinical isolates were consistent with their assignment to the genus Nocardia: whole cell hydrolysates contain meso-diaminopimelic acid as the diagnostic diamino acid of peptidoglycan; the whole-cell sugars are arabinose and galactose; the predominant phospholipids include diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol; MK-8-(H4) ω-cyc as the major menaquinone; mycolic acids ranging from 38 to 62 carbon atoms; and palmitic acid, tuberculostearic acid, palmitelaidic acid and oleic acid are the major fatty acids. Genus and species specific profiles were obtained following analysis by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectra of the clinical isolates. All isolates were found to be intermediately resistant or resistant to minocycline and resistant to ciprofloxacin but were susceptible to amikacin, imipenem and linezolid. Our polyphasic analysis suggest the three clinical isolates obtained from patients in Arizona represent a novel species of Nocardia for which we propose the name Nocardia arizonensis, with strain W9405(T) (=DSM 45748(T) = CCUG 62754(T) = NBRC 108935(T)) as the type strain.
    No preview · Article · Oct 2015 · Antonie van Leeuwenhoek
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    ABSTRACT: A new actinomycete, designated HM 537T, was isolated from soil in Hamedan Province, Iran. Cell-wall hydrolysates of strain HM 537T contained meso-diaminopimelic acid, and whole-cell hydrolysates contained ribose, glucose, galactose, and rhamnose and traces of mannose. The main phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidyl ethanolamine, phosphatidylinositol and an unknown phospholipid. MK-9(H4), an unknown MK and MK-10(H4) were the predominant menaquinones. The major fatty acids included iso-C16:0, iso-C15:0, iso-C16:1 G and 9(?)-methyl C16:0. Strain HM 537T had the highest 16S rRNA gene sequence similarity to Saccharothrix hoggarensis DSM 45457T (99.5 %) and Saccharothrix saharensis DSM 45456T (99.0 %). DNA-DNA hybridization studies showed relatedness values of 13.8 ± 3.3 % with S. hoggarensis and 16.3 ± 3.5 % with S. saharensis. Based on the results of phenotypic and genotypic studies, strain HM 537T (DSM 45486T = UTMC 00537T = CCUG 63021T) is considered to represent a novel species of the genus Saccharothrix, for which the name Saccharothrix ecbatanensis is proposed.
    No preview · Article · Sep 2015 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: A novel Gram-stain-positive, endospore-forming bacterium, designated FJAT-18043T, was isolated from a soil sample of potato field in Xinjiang Autonomous Region, China. Cells were rods that were catalase-positive and motile by peritrichous flagella. The strain was found to grow at temperatures ranging from 20 to 45 °C (optimum 35 °C), and pH 6.0-10.0 (optimum pH 9) with 0-10 % (w/v) NaCl concentration (optimum NaCl 0%). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FJAT-18043T belonged to the genus Bacillus and exhibited 16S rRNA gene sequence similarities of 97.7 %, 97.6%, 97.2% and 97.2% with Bacillus eiseniae A1-2T, Bacillus horneckiae DSM 23495T, Bacillus gottheilii WCC 4585T and Bacillus purgationiresistens DS22T, rspectively. DNA-DNA relatedness between strain FJAT-18043T and B. eiseniae A1-2 T was lower than 70% (36.1%). The menaquinone was identified as MK-7 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids detected were anteiso-C15:0 (26.1 %), iso-C15:0 (35.1 %), iso-C16:0 (7.8 %), anteiso-C17:0 (6.8 %), C16:0 (4.6 %) and iso-C14:0 (5.0 %). The DNA G + C content was determined to be 48.8 mol%. Phenotypic, chemotaxonomic and genotypic properties clearly indicated that isolate FJAT-18043T represents a novel species within the genus Bacillus, for which the name Bacillus solani sp. nov. is proposed. The type strain is FJAT-18043T ( = DSM 29501T = CCTCC AB 2014277T).
    No preview · Article · Aug 2015 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: The species Archangium gephyra, Angiococcus disciformis, Cystobacter minus and Cystobacter violaceus are currently classified in three different genera of the order Myxococcales. The 16S rRNA gene sequences of the respective type strains show a similarity higher than 98.4 % and form a tight phylogenetic group. A dendrogram calculating the similarity of MALDI-TOF spectra confirmed the close relatedness of the four species which grouped in a monophyletic cluster in neighborhood to other Cystobacter species. The type strains shared similar fatty acid patterns of high complexity with iso-C15:0, C16:1 ω5c, and iso-C14:0-3OH as the major components. The vegetative cells of these species are uniformly long needle-shaped rods, the myxospores are short rods, ovoid or irregularly spherical thus differing from the myxospores of the species related to Cystobacter fuscus, the type species of this genus. Some enzymatic and hydrolyzing reactions of the type strains are described. As a result of the high relatedness and similarity of the four species it is proposed to place them into one genus. Due to phylogenetic and morphological distinctness they should be classified in a genus distinct from the genus Cystobacter as Archangium gephyra, Archangium disciforme comb. nov, Archangium minus comb. nov. and Archangium violaceum comb. nov. Since the family Archangiaceae Jahn 1924 AL has priority over the family Cystobacteraceae McCurdy 1970 AL it is proposed to assign the genera Archangium, Anaeromyxobacter, Cystobacter, Hyalangium, Melittangium and Stigmatella to the family Archangiaceae.
    No preview · Article · Aug 2015 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: A Gram-staining-positive actinobacterial strain Miq-4T was isolated form soil around Meighan wetland in the center of Iran. Strain Miq-4T was strictly aerobic, catalase and oxidase positive. The isolate grew between 3-15 % NaCl, at 20-40 °C and pH 6.0-11.0. The optimum NaCl, temperature and pH for growth were 7.0 %, 30 °C and 7.0-8.5, respectively. The cell wall of strain Miq-4T contained meso-diaminopimelic acid as diamino acid and glucose as whole-cell sugar. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. It synthesized cellular fatty acids of anteiso and iso-branched types, anteiso-C17:0, anteiso- C15:0, iso-C16:0, and the major respiratory quinone was MK-9(H4). The G+C content of the genomic DNA was 68.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences and characteristic patterns of 16S rRNA gene signature nucleotides revealed that strain Miq-4T belongs to the family Glycomycetaceae and showed the closest phylogenetic similarity with Haloglycomyces albus YIM 92370T (94.1%). On the basis of phylogenetic analysis, phenotypic and chemotaxonomic characteristics, a novel genus and species of the family Glycomycetaceae, Salininema proteoliyticum gen. nov., sp. nov., are proposed. The type strain is Miq-4T (= IBRC-M 10908T = LMG 28391T). We also propose the emendation of the family Glycomycetaceae in order to include features of the new genus.
    No preview · Article · Jul 2015 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: A novel gamma-radiation-resistant and Gram positive actinobacterium designated BMG8133T was isolated from a limestone collected in the Sahara desert of Tunisia. The strain produced dry pale pink colonies with an optimum growth at 35-40°C and pH 6.5-8.0. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine and one unspecified glycolipid. MK-9(H4) was the dominant menaquinone. Galactose and glucose was detected as diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids iso-C16:0 and iso-C15:0. The DNA G+C content of the new strain is 74.5 %. The 16S rRNA gene sequence showed 98.3 % as highest sequence identity with Geodermatophilus ruber species. Based on phenotypic results and 16S rRNA gene sequence analysis, strain BMG8133T is proposed to represent a novel species, Geodermatophilus sabuli. Type strain is BMG8133T (=DSM 46844T = CECT 8820T).
    No preview · Article · Jun 2015 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: A novel halotolerant actinomycete, strain Chem15T was isolated from soil around Inche-Broun hypersaline wetland and its taxonomic position was determined based on a polyphasic approach. Strain Chem15T was strictly aerobic and tolerated NaCl up to 12.5 %. The optimum temperature and pH for growth were 28-30 °C and pH 7.0-7.5, respectively. The cell wall of strain Chem15T contained meso-diaminopimelic acid as diaminoacid and galactose, arabinose and ribose as whole-cell sugars. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, and phosphatidylinositol mannosides. The cellular fatty acids profile consisted of C16:0, iso-C18:0, C18:0 10-methyl and C18:1ω9C, and the major respiratory quinone was MK-8(H4cycl). The G+C content of the genomic DNA was 68.0 mol %. The novel strain constituted a distinct phyletic line within the genus Nocardia based on the 16S rRNA gene sequence analysis and was closely associated with Nocardia sungurluensis DSM 45714T and Nocardia alba DSM 44684T (98.2 and 98.1% 16S rRNA gene sequence similarity, respectively). However DNA-DNA relatedness and phenotypic data demonstrated that strain Chem15T was clearly different from closely related species of the genus Nocardia. It is concluded that the organism should be classified as a novel species of the genus Nocardia, for which the name Nocardia halotolerans sp. nov is proposed. The type strain is Chem15T (=IBRC-M 10490T =LMG 28544T).
    No preview · Article · Jun 2015 · International Journal of Systematic and Evolutionary Microbiology
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    ABSTRACT: A novel halophilic actinobacterium strain, designated H8T, was isolated from a Saharan soil sample collected in El-Goléa, South Algeria. Strain H8T was identified as representing a new genus using a polyphasic taxonomic approach. Phylogenetic analysis revealed that strain H8T shared the highest degree of 16S rRNA gene sequence similarity with ‘Mzabimyces algeriensis’ DSM 46680T (93.0 %), Saccharopolyspora ghardaiensis DSM 45606T (91.2 %), Halopolyspora alba DSM 45976T (90.8 %) and Actinopolyspora mortivallis DSM 44261T (90.0 %). The strain was found to grow optimally at 28–35 °C, at pH 6.0–7.0, and in the presence of 15–25 % (w/v) NaCl. The substrate mycelium was observed to be well developed and fragmented in liquid medium and on solid medium. The aerial mycelium was observed to be moderately abundant and to form long chains with non-motile, smooth-surfaced and ovoid or spherical spores at maturity. The cell wall of strain H8T was found to contain meso-diaminopimelic acid. The whole-cell hydrolysates were found to mainly contain arabinose and galactose. The diagnostic phospholipid detected was phosphatidylcholine, and MK-9(H4), MK-9(H2) and MK-10(H2) were found to be the predominant menaquinones. The major cellular fatty acids were determined to be anteiso-C17:0 and iso-C15:0. The genomic DNA G+C content of strain H8T was determined to be 71.3 mol%. The genotypic and phenotypic data showed that the strain represents a novel genus and species, for which the name Bounagaea algeriensis gen. nov., sp. nov. is proposed, with the type strain H8T (=DSM 45966T = CECT 8470T).
    No preview · Article · Jun 2015 · Antonie van Leeuwenhoek
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    ABSTRACT: The taxonomic position of an aerobic actinobacterial strain, BMG841T, isolated from the Bulla Regia monument (Tunisia) and exhibiting a high resistance to gammaradiation (D10 ~9 kGy) was determined using polyphasic approach. The optimal growth range was found to be 25-35 °C at pH of 7.0-8.5. The strain was observed to form black dry colonies. Chemotaxonomic characteristics of the isolate showed a cell wall type III, with galactose and glucose as diagnostic sugars; phosphatidylcholine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid as main polar lipids; and MK-9(H4) as the predominant menaquinone. The major cellular fatty acids were identified as iso-C16:0 and isoC15:0. Phylogenetic analysis indicated that strain BMG841T represents a novel member of the genus Geodermatophilus with high 16S rRNA gene sequence identity with Geodermatophilus saharensis (98.28 %). Based on phylogenetic and phenotypic analysis, strain BMG841T is proposed as the type strain (= DSM 46841T = CECT 8821T) of a novel species, Geodermatophilus bullaregiensis.
    Full-text · Article · May 2015 · Antonie van Leeuwenhoek
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    ABSTRACT: Most bacteria contain a peptidoglycan (PG) cell wall, which is critical for maintenance of shape and important for cell division. In contrast, Planctomycetes have been proposed to produce a proteinaceous cell wall devoid of PG. The apparent absence of PG has been used as an argument for the putative planctomycetal ancestry of all bacterial lineages. Here we show, employing multiple bioinformatic methods, that planctomycetal genomes encode proteins required for PG synthesis. Furthermore, we biochemically demonstrate the presence of the sugar and the peptide components of PG in Planctomycetes. In addition, light and electron microscopic experiments reveal planctomycetal PG sacculi that are susceptible to lysozyme treatment. Finally, cryo-electron tomography demonstrates that Planctomycetes possess a typical PG cell wall and that their cellular architecture is thus more similar to that of other Gram-negative bacteria. Our findings shed new light on the cellular architecture and cell division of the maverick Planctomycetes.
    Full-text · Article · May 2015 · Nature Communications

Publication Stats

9k Citations
1,070.84 Total Impact Points

Institutions

  • 1993-2015
    • Leibniz Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
      Brunswyck, Lower Saxony, Germany
  • 2014
    • Eötvös Loránd University
      • Department of Microbiology
      Budapeŝto, Budapest, Hungary
  • 2013
    • Helmholtz Centre for Infection Research
      Brunswyck, Lower Saxony, Germany
  • 2009
    • Korea Research Institute of Bioscience and Biotechnology KRIBB
      • Biological Resource Center
      Anzan, Gyeonggi-do, South Korea
  • 2008
    • Université Paris-Sud 11
      Orsay, Île-de-France, France
  • 2004-2006
    • Universidad de Salamanca
      • Department of Microbiology and Genetics
      Helmantica, Castille and León, Spain
    • Seoul National University
      • Department of Biological Sciences
      Seoul, Seoul, South Korea
    • Yunnan University
      • The Key Laboratory for Microbial Resources of the Ministry of Education
      Yün-nan, Yunnan, China
  • 2005
    • Bundesanstalt für Geowissenschaften und Rohstoffe
      Hanover, Lower Saxony, Germany
  • 2003
    • Russian Academy of Sciences
      • Institute of Microbiology
      Moscow, Moscow, Russia
  • 2002
    • University of Vienna
      Wien, Vienna, Austria
    • Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute
      Jena, Thuringia, Germany
    • Nanyang Technological University
      • Division of Environmental and Water Resources (EWRE)
      Tumasik, Singapore
    • University of Coimbra
      • Department of Chemistry
      Coímbra, Coimbra, Portugal
  • 2001
    • The Leibniz Institute for Natural Product Research and Infection Biology
      Jena, Thuringia, Germany