M Fazio

Istituto Dermopatico dell'Immacolata, Roma, Latium, Italy

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Publications (41)52.16 Total impact

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    ABSTRACT: Background/aims: Comeometry represents a useful tool for dermatologists, even though several interfering variables may reduce its reliability level. The present study describes some of the methodological points that optimize corneometric measurements and analyzes the interference due to the skin area selected, the age and the sex of the individuals observed.Results: The results show that skin areas may present significantly different corneometric values. In addition, a significant correlation of the corneometric values at most of the sites was observed independently of the sun-exposed site.Conclusions: The corneometric values were clearly age dependent and sex independent. Measurements were obtained from eight different body areas (two of them were symmetric and presented superimposable results). The volar forearm appears to be the area least influenced by the patients’age and presents the lowest correlation coefficients with other skin sites.
    No preview · Article · Oct 2006 · Skin Research and Technology
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    ABSTRACT: No abstract is available for this article.
    No preview · Article · Aug 2005 · Clinical and Experimental Dermatology

  • No preview · Article · Feb 1999 · Dermatology
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    ABSTRACT: Endothelins (ETs), in addition to their systematical activities, exert important functions at the skin level, such as increase of keratinocyte proliferation, neo-angiogenesis and leukocyte chemotaxis, which are among the main characteristics of psoriasis. To assess a possible ET-1 involvement in plaque-type psoriasis, ET-1 determinations were carried out in 15 sera and 8 lesional and non-lesional biopsy skin extracts from psoriatic patients and in 15 sera and 5 biopsy skin extracts from healthy volunteers, sex- and age-matched, using commercially available ELISA kits. A statistical analysis of the results showed that ET-1 levels were increased in sera of psoriatic patients, as compared to normal subjects (p = 0.04). In addition, there was a significant correlation between both serum (r = 0.60, p = 0.02) and lesional skin (r = 0.80, p = 0.03) ET-1 values versus the Psoriasis Area and Severity Index scores. Significant increases of the lesional versus the non-lesional (p = 0.01) and versus the normal (p = 0.04) ET-1 skin extract values were observed, together with a significant correlation between lesional and non-lesional ET-1 skin levels (r = 0.79, p = 0.03). These findings were also confirmed at the mRNA level, using RT-PCR analysis, where increased ET-1 mRNA levels, densitometrically measured, were found in the lesional samples versus non-lesional and normal skin. Since interleukin-8 is involved in psoriasis and shares some biological properties with ET-1, we further evaluated the levels of this cytokine in skin extracts. The behaviour of interleukin-8 paralleled that of ET-1, and a significant correlation between these two molecules was observed in the lesional skin (r = 0.76, p = 0.05). Taken together, these data stress that, as previously described for interleukin-8, ET-1 may be involved in inflammatory processes associated with psoriasis.
    Full-text · Article · Jan 1998 · Acta Dermato Venereologica
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    ABSTRACT: The clinical and histological features of the entities known as 'white fibrous papulosis of the neck' (WFPN) and 'acquired elastolysis of the papillary dermis simulating pseudoxanthoma elasticum' (PDE) are not clearly defined. This study was conducted to compare our experience of WFPN/PDE with those described in the literature. Twenty patients presented at our institution with papular eruptions involving the neck. The asymptomatic lesions, which ranged in colour from normal skin tones to yellowish, were isolated or coalescent. Microscopically, the papules showed elastolysis and fibrosis of the upper reticular and papillary dermis. A review of the literature shows similar characteristics in cases reported as WFPN and PDE. This study indicates that WFPN and PDE are variants of a single disorder that can be more precisely defined as 'fibroelastolytic papulosis of the neck' and which appears to be a manifestation of intrinsic skin ageing.
    No preview · Article · Oct 1997 · British Journal of Dermatology

  • No preview · Article · Sep 1997 · Journal of the European Academy of Dermatology and Venereology

  • No preview · Article · Sep 1997 · Journal of the European Academy of Dermatology and Venereology
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    ABSTRACT: TNF-alpha is a pleiotropic cytokine possibly involved in the pathogenesis of psoriasis. Objective: to analyze the serum TNF-alpha levels in plaque-type psoriatic patients to evaluate the concentrations, correlation with the severity score and behaviour after therapy. The serum TNF-alpha levels of thirty-seven patients (25 females and 12 males; median age: 52.5 years, range 18-81: median PASI score: 11.4, range 3.5-42) and thirty healthy controls (21 females and 9 males, median age: 48.5 years, range 25-77) were compared after measurements obtained employing commercially available ELISA kits. The median serum TNF-alpha levels of the patients were significantly higher than those of controls (p = 0.004). 30/37 patients were followed over time at 2 and 4 weeks of treatment. Twenty one subjects were also observed after 6 weeks. After effective treatments, both the PASI scores and the cytokine levels were significantly and concomitantly reduced (p < 0.001). Significant correlations were found when the TNF-alpha values were plotted against the PASI scores both at the time of patient enrollment and at all the subsequent times (118 observations). A significant correlation was observed between circulating TNF-alpha and sE-selectin in agreement with a possible functional activity of the cytokine. However, no correlation was found between the cytokine levels and other 4 soluble membrane molecules. Our findings indicate that the molecule studied, although non specific for the disease considered, presents a behaviour paralleling that of the disease severity and therefore might have clinical usefulness, particularly in monitoring the therapeutic effects.
    No preview · Article · Jul 1997 · Journal of biological regulators and homeostatic agents
  • A Mussi · M Carducci · C Bonifati · M Fazio · F Ameglio

    No preview · Article · Jun 1997 · Acta Dermato Venereologica
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    ABSTRACT: Increased levels of several cytokines, mainly proinflammatory mediators, have been reported in psoriatic lesions. Little information, if any, is available concerning other cytokines, especially those initially studied as marrow differentiation agents. Using the experimental approach of measuring cytokines released by skin organ cultures. IL-11 and three other proinflammatory cytokines (IL-1β, IL-6 and IL-8) were determined using commercially available ELISA kits in supernatants of ten biopsies from lesional and nonlesional psoriatic skin areas and in supernatants of biopsies from ten normal volunteers. The results obtained showed that the amounts of IL-11 and the other three modulators were significantly increased in the material from the lesional areas ( P < 0.01). The amounts of IL-11, which is known to have functional activity similar to the proinflammatory cytokines and to share a receptor component with IL-6, were also correlated with the disease severity index ( R = 0.69, P = 0.04). In addition, a nearly significant correlation was noted between the amounts of IL-11 released by the lesional and the nonlesional skin biopsies ( R = 0.66, P = 0.05). More detailed studies are needed to clarify whether IL-11 plays a specific functional role in psoriasis, but this study emphasizes the complexity of the pathogenesis of psoriasis and the cytokine network, including activation of proinflammatory and haemopoietic biological response modifiers, in this disease.
    No preview · Article · Jun 1997 · Archives for Dermatological Research
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    ABSTRACT: Several cytokines have been shown to be increased in psoriasis, mainly at the local and sometimes at the systemic level. At present, no data concerning the relationships between psoriasis and interleukin-7 (IL-7) are available. This biological modifier regulates immune response by means of its pleomorphic activities, including the ability to stimulate different monocyte functions, such as killing of intracellular pathogens, induction of cytokines, and enhancement of some membrane molecule expression. Study groups consisted of nine psoriatic and nine normal subjects. Using a commercially available immune-enzyme method, IL-7 concentrations were determined in various samples: biopsy and scale extracts, peripheral blood mononuclear cells (PBMCs) supernatants, and sera. The results show that IL-7 levels are significantly increased both in biopsy and in scale extracts obtained from lesional skin compared to those obtained from nonlesional and normal skin (P at least < 0.01). In addition, the serum values were higher in psoriatic patients than in the controls (P = 0.003). In contrast, no significant differences were observed in the supernatants of unstimulated PBMCs maintained in culture for 48 hr. These data suggest that IL-7 is involved in some way in the pathomechanisms of psoriasis and that the keratinocyte may be a candidate for psoriatic IL-7 overproduction.
    No preview · Article · May 1997 · Clinical Immunology and Immunopathology
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    ABSTRACT: Corneometry has been considered useful both to evaluate disease severity and to monitor psoriatic patients during treatment. However, a limitation of this technique is that the patient's age influences the corneometric determinations, thus reducing their clinical usefulness. The aim of this study was, therefore, to establish whether age normalization of the corneometric results may provide more reliable data for clinical use. Corneometric levels were determined in 10 plaque-type psoriatic patients, under standard conditions. Eight serum variables, including transforming growth factor-beta 1 and seven soluble membrane molecules, were assayed with commercially available immune-enzyme methods in the same patients, whose age and PASI scores were also recorded. The age normalization procedure improved all the correlation coefficients calculated on the lesional or non-lesional corneometric values versus the PASI scores as well as versus the other serum variables. This approach may render corneometric determinations more useful to evaluate disease status or treatment effect in patient groups with plaque-type psoriasis.
    No preview · Article · Apr 1997 · Acta Dermato Venereologica
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    ABSTRACT: Endothelins (ETs) are members of a 21 amino acid peptide family represented by ET-1, ET-2 and ET-3, having profound cardiovascular, mitogenic and neuroregulatory actions. These peptides are secreted by different cell types, including human keratinocytes, which in turn express ET-A receptors. Due to the ability of these molecules to stimulate the keratinocyte proliferation, neo-angiogenesis and leukocyte chemotaxis (some the main characteristics of psoriasis), we measured the levels of one of these mediators, namely ET-1, in the sera of a group of patients with active plaque-type psoriasis and in another group of healthy subjects. Our results confirmed that serum ET-1 levels were greater in psoriatic patients than in healthy controls (p < 0.05) and also showed a significant correlation between these levels and the psoriasis area and severity index (PASI) scores (R = 0.6, p < 0.05). These data, taken all together, suggest a possible involvement of ET-1 in the pathogenesis of psoriasis.
    No preview · Article · Jan 1997 · International journal of immunopathology and pharmacology
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    ABSTRACT: In order to evaluate a possible presence of a systemic lipoperoxidative process in psoriasis we have determined the pattern of polyunsaturated fatty acid (PUFA) of plasma phospholipids and the plasma levels of vitamin E (Vit. E) in patients affected with plaque-type disease. The results were correlated with the serum levels of some molecules involved in the inflammatory process, namely IL-6, sL-selectin, IL1ra and TGFβ1. A significant correlation was observed between increase of pro-inflammatory cytokines IL6 and sL-selectin and decrease of Vit. E and PUFA respectively. On the contrary a positive correlation between IL1ra and TGFβ1 and PUFA concentrations was found. Moreover, in order to study the involvement of enzymatic antioxidants, red blood cells superoxide dismutase (SOD) and catalase (CAT) activity were evaluated and an increase of SOD activity was detected. Our results suggest that in patients affected with psoriasis an imbalance of antioxidant system, correlated with the production of pro-inflammatory cytokines, could exist.
    No preview · Article · Jan 1997 · International journal of immunopathology and pharmacology
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    ABSTRACT: The surface area and micropore distribution of the porous glass prepared from borosilicate glass were controlled by the addition of metal oxides. When alumina or zirconia was added to the borosilicate glass, the micropore size distribution was very sharp and the maximum of the distribution was located at 1–2 nm. The addition of manganese oxide or antimonial oxide did not produce a sharp micropore distribution in the porous glass. When the hydrogenation of benzenes, substituted with a different number of methyl groups, was carried out over nickel catalysts supported on the porous glass prepared from the borosilicate glass with zirconia, the hydrogenation rate decreased according to the number of methyl groups and the position of the groups. These results indicate that a shape-selective nickel catalyst can be prepared from the porous glass synthesized from the borosilicate glass with a small amount of metal oxide.
    No preview · Article · Jun 1996 · Microporous Materials
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    ABSTRACT: The serum transforming growth factor-β1 (TGF-β1), the soluble intercellular adhesion molecule (sICAM-1), the soluble E-selectin (sE-selectin), the soluble L-selectin (sL-selectin) and the interleukin-1 receptor antagonist (IL-1Ra) levels were determined in 15 samples of psoriatic serum using commercially available ELISA kits. The median serum TGF-β1 levels were significantly increased as compared to the 21 healthy subjects and a significant correlation with the PASI scores was observed, suggesting an involvement of this cytokine in the pathogenic mechanisms of plaque-type psoriasis. Despite 4 weeks of effective therapy and a subsequent drop in the PASI scores, no significant TGF-β1 modifications were observed in the treated patients, suggesting a persistence of the raised levels of this molecule in the blood. This has been previously shown for other molecules, suggesting a time dissociation between TGF-β1 metabolism and recovery. Significant correlations were found with the serum TGF-β1, sICAM-1, sE-selectin, sL-selectin and IL-1Ra levels, whose interrelationships with TGF-β1 have been previously reported in the literature. This indirectly suggests that the amounts of serum TGF-β1, although altered, are biologically coordinated and may be active, exerting functions known to be TGF-β1-dependent such as monocyte stimulation, neoangionesis and fibroblast activation.
    No preview · Article · Jan 1996
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    ABSTRACT: The authors present a case of a woman with Dowling-Degos disease in which the reticular pigmentation clearly involved the perianal and vulvar area. The extreme rarity of this localization and the importance of recognizing this disease in the mucosa is emphasized to permit identifying forms with few cutaneous characteristics and to distinguish them from other dermatoses.
    No preview · Article · Dec 1995 · Journal of the European Academy of Dermatology and Venereology
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    ABSTRACT: Increased tumour necrosis factor alpha has been found in psoriatic skin. This cytokine activates endothelial cells and induces the membrane E-selectin molecule (E-selectin or endothelial leucocyte adhesion molecule 1); the same cytokine is able to induce its own receptors. Since the soluble forms of E-selectin and tumour necrosis factor receptor (TNF-R, 60 kD) may be reliably measured in body fluids, these determinations have been performed in the sera of psoriatic patients. To evaluate endothelial activation in psoriatic patients, sE-selectin has been determined in patient sera and compared with those of a control group. sTNF-R (60 kD) was also measured in the same samples. Two commercially available enzyme immunoassay methods have been used to determine sE-selectin and sTNF-R (60 kD) in the sera of 19 patients with plaque-type psoriasis; 22 healthy subjects were used as controls. Significantly increased amounts of sE-selectin serum levels were found in psoriatic patients as compared to healthy controls. Moreover, a direct correlation between sE-selectin and PASI scores was observed. On the contrary, sTNF-R (60 kD) serum levels presented no increases. These data suggest that sE-selectin serum levels are a reliable marker of disease activity in psoriatic patients.
    No preview · Article · Feb 1995 · Dermatology
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    ABSTRACT: Hereditary sclerosing poikiloderma is a genodermatosis with dominant autosomal transmission and variable penetration. The first case was described by Weary in 1969 in 7 members of two black families. A 10-year-old girl had localized regional poikiloderma of the fingers and club toes. These lesions were associated secondarily with linear symmetric bands of sclerotic tissue in the axiallary regions. On the X-ray examinations of the distal phalanges of the fingers and the toes showed a proximal growth foyer and absent ungueal phalanges, excepting in the fourth finger of the left hand. Capillaroscopy of the supra-ungueal fold of the fingers showed abnormal capillary circulation. Histology and ultrastructural examinations did not reveal any pathognomonic alterations. This case is the first reported in a white patient. The radiological aspect and the results of the capillaroscopy of the fingers and the toes have not been reported previously in this rare genodermatosis. Inheritance of this genodermatosis is poorly defined.
    No preview · Article · Feb 1995 · Annales de Dermatologie et de Vénéréologie
  • M. Carducci · A. Mussi · C. Bonifati · M. Fazio

    No preview · Article · Jan 1995