[Show abstract][Hide abstract] ABSTRACT: Anti-HLA antibodies is one of important factors which affect humoral rejection against graft as well as efficacy of platelets transfusion in allogeneic bone marrow transplantation (alloBMT). Here, we had a patient who produced anti-HLA antibodies following alloBMT. These anti-HLA antibodies was considered to be derived from the donor registered in Japan Marrow Donor Program, who had anti-HLA antibodies. The patient was male in 20-years generation. He was diagnosed as philadelphia chromosome-positive acute lymphoblastic leukemia in February 2009. He achieved remission after induction chemotherapy. He underwent BMT on July 31 in the same year. Anti-HLA antibodies was detected in the patient two months after that transplantation. These antibodies exhibited the same specific reactivity against HLA-A and B antigens as donor. It is important to observe the patient by checking these antibodies periodically. Because platelets play a major role in transfusion following alloBMT. Once anti-HLA antibodies is detected in a recipient, we should choose platelets not reacting with the antibodies of donor.
No preview · Article · Jan 2014 · Japanese Journal of Transfusion and Cell Therapy
[Show abstract][Hide abstract] ABSTRACT: The levels of interleukin-6 and platelet-derived microparticles (PMPs) were measured in the blood of 137 patients with side effects from platelet concentrate (PC) transfusion with leukocyte removal filtration, P-selectin-expressing platelet and PMPs in stored PC before and after the filtration, and filtered leukocytes positive for P-selectin glycoprotein ligand-1. The side effects, which were observed in 203 transfusions for 84 patients with hematologic disease and 53 patients with nonhematologic disease with no significant difference between the two groups, included urticaria (75.9%), erythema (18.7%), and fever (17.2%), but no anaphylactic reactions. The levels of interleukin-6 and PMP correlated in both groups, and were significantly higher in the hematologic disease group than in the nonhematologic disease group. The level of PMP, but not interleukin-6, was significantly higher for patients testing positive for allergic reaction than for those testing negative. In the stored PC prior to filtration, the level of interleukin-6 was normal. The level of P-selectin-expressing platelets and PMPs was elevated before filtration, but was significantly lower after filtration. Taken together, the results suggest that PMP is involved in the generation of transfusion reactions, and indicate that both platelets and PMP displaying P-selectin bind to P-selectin glycoprotein ligand-1 of leukocytes retained by the leukocyte filter.
No preview · Article · Nov 2000 · Clinical and Applied Thrombosis/Hemostasis