M F Reznikoff-Etievant

Institut National de la Transfusion Sanguine, Paris, Lutetia Parisorum, Île-de-France, France

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Publications (38)320.28 Total impact

  • M. F. Reznikoff-Etievant · C. Dangu · R. Lobet
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    ABSTRACT: HLA-A-B-C and DR typing was performed on sixteen individuals whose PLA1 platelet group was negative. Ten of them were immunostimulated with PLA1 antigen by pregnancy or transfusion; six of them were responders and developed an anti-PLA1 antibody. A high incidence of HLA-B8 antigen was observed in that group of responders. This observation is supported by five other cases of allo-immunization reported in previous papers.
    No preview · Article · Dec 2008 · Tissue Antigens
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    Gang‐Ming Zou · M F Reznikoff-Etievant · Francois Hirsch · Jacques Milliez
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    ABSTRACT: It has been reported that interferon (IFN)-gamma should inhibit in vitro mouse embryo growth by direct cell toxicity. However, the mechanism involved has not been clearly established. In the present study, this question was addressed using the embryonic stem (ES) cell model. It was found that IFN-gamma, induces a dose-dependent apoptosis in ES cells, as assessed by trypan-blue staining, by Annexin-V labeling and DNA analysis, Moreover, IFN-gamma treatment cooperates with Fas-mediated apoptosis, a phenomenon that has been recently reported. As Bcl-2 oncoprotein functions as a death repressor molecule in an evolutionarily conserved cell death pathway, its expression was analyzed by flow cytometry. It was demonstrated that Bcl-2 is expressed in ES cells. When compared to untreated ES cells, IFN-gamma-treated, apoptotic cells expressed a lower Bcl-2 level and a normal level of Fas, whereas surviving cells expressed a normal level of Bcl-2 but a lower Fas expression. Altogether, these data suggest that IFN-gamma may influence early mouse embryo development by promoting apoptosis, which may constitute a novel mechanism of IFN-gamma embryotoxicity.
    Full-text · Article · Jul 2000 · Development Growth and Regeneration
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    ABSTRACT: Fas antigen (APO-1/CD95) can regulate the activity of various cells during adulthood. This study aimed at determining whether Fas may also be involved in the regulation of very early events such as the embryo preimplantation stage. We used mouse embryo stem (ES) cell line as a model for testing the effect of Fas crosslinking upon anti-Fas monoclonal antibody (MoAb) treatment. In addition, this treatment was also applied to in-vitro mouse-embryo culture. Flow-cytometry analysis of cultured ES cells demonstrated an increase in Fas expression. unchanged in the presence of mouse interleukin-2, while greatly upregulated in the presence of lipopolysaccharide (LPS). As determined by various means, ES cells may undergo a Fas-mediated apoptosis, slightly but significantly intensified by the addition of LPS to cell cultures. We also report that anti-Fas MoAb directly inhibited two-cell stage mouse-embryo (preimplantation) development in in-vitro culture conditions. These data suggest a novel mechanism controlling the regulation of physiological cell turnover as well as blastocyst implantation in early embryo development.
    No preview · Article · May 2000 · American journal of reproductive immunology (New York, N.Y.: 1989)
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    M F Reznikoff-Etievant · V Cayol · G M Zou · N Abuaf · A Robert · C Johanet · J Milliez
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    ABSTRACT: The objective of this study of patients with habitual abortion (HA), was to determine their autoimmune profile and to try to prevent new abortions using low-dose aspirin for 7 months with prednisone in the first trimester only, or with low-dose aspirin alone. A total of 678 healthy patients with three or more HA were investigated for antiphospholipid antibodies, antinuclear and antithyroid antibodies. Among these patients, 277 pregnant women were treated, 214 were given prednisone and aspirin (161 autoantibody-negative and 53 autoantibody-positive women), and 63 autoantibody-negative women received aspirin alone. Autoantibodies were present in 33.9% of the patients, in 82.6% of them anticardiolipin antibodies were found to be isolated or associated with antiprothrombin, antithyroid, circulating anticoagulant, antinuclear or anti-beta2 glycoprotein 1 antibodies. In autoantibody-negative pregnant women treated by prednisone and aspirin or aspirin alone, the success rate of live births was 90.7% (146 out of 161) and 74.6% (47 out of 63) respectively (P < 0.01). In autoantibody-positive patients treated with prednisone and aspirin the success rate was 84.9% (45 out of 53) (not significant). Prednisone and aspirin seemed to be as efficient in autoantibody-negative or positive women but better than aspirin alone in autoantibody-negative women. A double-blind trial is in progress to confirm these results.
    Full-text · Article · Sep 1999 · Human Reproduction
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    M.F. Reznikoff-Etievant
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    ABSTRACT: The objective of this study of patients with habitual abortion (HA), was to determine their autoimmune profile and to try to prevent new abortions using low-dose aspirin for 7 months with prednisone in the first trimester only, or with low-dose aspirin alone. A total of 678 healthy patients with three or more HA were investigated for antiphospholipid antibodies, antinuclear and antithyroid antibodies. Among these patients, 277 pregnant women were treated, 214 were given prednisone and aspirin (161 autoantibody-negative and 53 autoantibody-positive women), and 63 autoantibody-negative women received aspirin alone. Autoantibodies were present in 33.9% of the patients, in 82.6% of them anticardiolipin antibodies were found to be isolated or associated with antiprothrombin, antithyroid, circulating anticoagulant, antinuclear or anti-β2 glycoprotein 1 antibodies. In autoantibody-negative pregnant women treated by prednisone and aspirin or aspirin alone, the success rate of live births was 90.7% (146 out of 161) and 74.6% (47 out of 63) respectively (P < 0.01). In autoantibody-positive patients treated with prednisone and aspirin the success rate was 84.9% (45 out of 53) (not significant). Prednisone and aspirin seemed to be as efficient in autoantibody-negative or positive women but better than aspirin alone in autoantibody-negative women. A double-blind trial is in progress to confirm these results.
    Preview · Article · Aug 1999 · Human Reproduction
  • J Yao · J Milliez · A Netter · C Roux · M F Reznikoff-Etievant
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    ABSTRACT: About 30% of recurrent spontaneous abortions remain unexplained by traditional or biological anomalies. The purpose of this work was to investigate embryotoxic factors produced by trophoblast stimulated lymphocytes from women with unexplained recurrent spontaneous abortion. The samples from 36 women with recurrent abortion before and during the next pregnancy and from 7 women with normal pregnancies and no history of spontaneous abortion have been tested. The lymphocytes were stimulated with trophoblastic extracts. The supernatants of the stimulated lymphocytes were tested in a 4-cell mouse embryo culture. The secretion of embryotoxic factor was determined if the number of life blastocysts was less than 50% of control values after 4 days. The lymphocytes from 59% women with 3 or more recurrent abortion produced the embryotoxic factor, this factor may be useful in predicting pregnancy outcome in women with a history of unexplained recurrent miscarriage. The embryotoxic factor might be a new cause of recurrent abortion and a predictive factor.
    No preview · Article · Feb 1997 · Journal de Gynécologie Obstétrique et Biologie de la Reproduction
  • I Mansour · M F Reznikoff-Etievant · A Netter
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    ABSTRACT: The expression of the progesterone receptor in human peripheral blood lymphocytes was analysed, using an enzyme linked immunosorbent assay (Abbott PgR-EIA monoclonal), in order to evaluate its prognostic character in the context of spontaneous abortion. Cytosols were prepared from lymphocytes of 24 healthy pregnant women (11 first, 10 second and three third trimester), seven healthy non-pregnant women, nine women with recurrent spontaneous abortion, and six healthy men. In addition, a human breast carcinoma cell line (ZR-75-1), which expresses the progesterone receptor, was analysed throughout. The ZR-75-1 cell line showed an expression of 642 fmol/mg whereas lymphocytes of pregnant women showed an expression < or = 4 fmol/mg. Lymphocytes of non-pregnant women, women with threatened pre-term delivery, and men showed equivalent levels: 3 +/- 1, 3 +/- 2 and 5 +/- 4 fmol/mg respectively. These results show that there is no evidence of specific expression of the progesterone receptor in pregnancy and exclude any prognostic character in spontaneous abortion. A role for the progesterone receptor in the mechanism of the known effect of progesterone on peripheral blood lymphocytes is also excluded.
    No preview · Article · Aug 1994 · Human Reproduction
  • M F Reznikoff-Etievant · Y de Lachaux · L. de Marpeau · Couroucé AM

    No preview · Article · Nov 1992 · The Lancet
  • I Mansour · M.A. Jarraya · A Netter · L Marpeau · M.F. Reznikoff-Etievant

    No preview · Article · Nov 1992 · The Lancet
  • M.F. REZNIKOFF-ETIEVANT · J.C. BONNEAU · D Alcalay · B Cavelier · C Touré · R Lobet · Netter AI
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    ABSTRACT: Previously, several groups reported an increase in HLA antigen-sharing in couples suffering from unexplained repeated spontaneous abortions. It was felt necessary to find out if HLA sharing could have any effect on children born after a successful pregnancy. The birthweight figures of children of 76 couples with repeated spontaneous abortions were analyzed. The results show a significantly lower birthweight in babies born from those couples, presenting a high incidence of HLA antigen-sharing, particularly concerning class II antigens.
    No preview · Article · Feb 1991 · American journal of reproductive immunology (New York, N.Y.: 1989)
  • N Valentin · A Vergracht · J.D. Bignon · M.L. Cheneau · D Blanchard · C Kaplan · M.F. Reznikoff-Etievant · J.Y. Muller
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    ABSTRACT: The alloimmunization against the platelet PL-A1 antigen is strongly associated with a HLA class II structure in mothers of thrombocytopenic neonates. Most of the immunized women have first been shown to possess the DR3 specificity and subsequently the DRw52 allele. The 18 immunized mothers studied here by restriction fragment length polymorphism analysis had the DRw52a specificity at the DRB3 locus whatever their HLA-DRB1 gene product. This finding strongly suggests that the DRB3 chain is directly involved in the presentation of the PL-A1 antigen to the specific T cell. In addition, the similarities between DR3 and DRw52 structures due to a hypothetical gene conversion event should be considered in order to understand the high frequency of DR3 among the DRw52a-responding women. Alternatively, the high frequency of DR3 among the DRw52-responding mothers might be due to the high responder status associated with the former specificity.
    No preview · Article · Mar 1990 · Human Immunology
  • Julia Szekeres-Bartho · M F Reznikoff-Etievant · P Varga · M F Pichon · Z Varga · G Chaouat
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    ABSTRACT: The progesterone receptor-specific monoclonal antibody (MoAb) mPRI was tested for its reactivity towards peripheral blood lymphocytes (PBL) of 49 healthy pregnant women, nine pregnant women with clinical symptoms of threatened preterm delivery, seven women with recurrent spontaneous abortion, ten women in labour and ten women with spontaneous abortion. Lymphocytes of 12 healthy age-matched non-pregnant volunteers were used as controls. Lymphocytes of nine healthy pregnant women at the 1st trimester of pregnancy and those of two non-pregnant donors were tested for the presence of estrogen and progesterone receptors by enzyme immunoassay. PBL of healthy pregnant women contained significantly more positive cells than those of non-pregnant controls. Furthermore, the number of receptor-containing cells increased in parallel with gestational age. In blood samples drawn during labour, as well as in those obtained from women with spontaneous abortion or clinical symptoms of threatened pre-term delivery, the percentage of positively stained lymphocytes was significantly lower than normal pregnancy values. This was also the case in peripheral blood of pregnant women with a history of recurrent spontaneous abortions.
    No preview · Article · Jan 1990 · Journal of Reproductive Immunology
  • Ma. Jarraya · M.F. Reznikoff-Etievant · I. Mansour · R. Lobet · C. Toure · Ch. Salmon · A. Netter

    No preview · Article · Jul 1989
  • M.F. Reznikoff-Etievant · M.A. Jarraya · A. Netter · J. Huchet

    No preview · Article · Jul 1989 · Journal of Reproductive Immunology
  • M.F. Reznikoff-Etievant · R. Lobet · V. De Lachaux · A. Netter · R. Frydman

    No preview · Article · Jul 1989
  • J. Szekeres-Bartho · M.F. Reznikoff-Etievant · P. Varga · Z. Varga · G. Chaouat

    No preview · Article · Jul 1989 · Journal of Reproductive Immunology
  • M F Reznikoff-Etievant · C Kaplan · J Y Muller · F Daffos · F Forestier

    No preview · Article · Feb 1988 · Current studies in hematology and blood transfusion
  • J Y Muller · C Kaplan · M F Reznikoff-Etiévant · C Patereau · F Daffos · F Forestier · M C De Puy Montbrun · D Lyon-Caen · C Salmon

    No preview · Article · Feb 1988 · Current studies in hematology and blood transfusion
  • M F Reznikoff-Etievant · Isabelle Durieux · Jacques Huchet · Charles Salmon · Albert Netter

    No preview · Article · Jan 1988 · The Lancet
  • M. F. Reznikoff-Etievant · I. Durieux · J. Huchet · C. Salmon · A. Netter
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    ABSTRACT: A large proportion of recurrent spontaneous abortions (RSAs) remain unexplained; but recently many observations during both normal and pathological pregnancies underline the possibility of an immunological aetiology.
    No preview · Chapter · Jan 1988