K. Javidnia

Shiraz University of Medical Sciences, Chimaz, Fars, Iran

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Publications (55)50.21 Total impact

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    ABSTRACT: Ohmic-assisted hydrodistillation (OAHD) is a new method proposed for extraction of essential oils in which ohmic heating technology is combined with distillation. In this study, different frequencies (25, 50 and 100 Hz) as well as high and low intensity OAHD (220 and 380 Volt) were applied on aerial parts of Mentha piperita L. and the results were compared with those of the conventional hydrodistillation (HD). The results showed that high and low intensity OAHD methods had the extraction times of 13.54 and 19.71 min, respectively, while this value was about one hour for HD. Scanning electron micrographs of mint showed a sudden eruption of essential Oil glands for all OAHDs samples. GC-MS analysis did not indicate any noticeable changes in the compounds of all extracted essential oils. The results of this study showed that higher applied voltage can speed up OAHD and confirmed this emerging technology as a green technology (considering fossil fuels as the main source of electrical energy).
    Full-text · Article · Feb 2015 · Innovative Food Science & Emerging Technologies
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    ABSTRACT: Ohmic and microwave assisted hydrodistillation (OAHD and MAHD, respectively) are advanced hydrodistillation (HD) techniques utilizing ohmic and microwave heating processes for extraction of essential oils. OAHD and MAHD of essential oils from the aerial parts of peppermint were studied and the results were compared with those of the traditional HD. The results showed that OAHD and MAHD methods require less than half an hour for extraction process while HD require about one hour. Scanning electron microscopy of mint leaves undergone OAHD and MAHD provided evidences as to a sudden rupture of essential oil glands. GC-MS analysis did not indicate any noticeable changes in the compounds of the essential oils obtained by novel studied methods in comparison with HD. The results introduced OAHD as the greenest technique in terms of energy consumption. MAHD was superior in terms of rate of essential oils accumulation and also extraction duration parameter.
    Full-text · Article · Jan 2015 · Food and Bioproducts Processing
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    ABSTRACT: Ohmic-assisted hydrodistillation (OAHD) is a new method proposed for extraction of essential oils in which ohmic heating technology is combined with distillation. In this study, different frequencies (25, 50 and 100 Hz) as well as high and low intensity OAHD (220 and 380 V) were applied on aerial parts of Mentha piperita L. and the results were compared with those of the conventional hydrodistillation (HD). The results showed that high and low intensity OAHD methods had the extraction times of 13.54 and 19.71 min, respectively, while this value was about 1 h for HD. Scanning electron micrographs of mint showed a sudden eruption of essential oil glands for all OAHD samples. GC–MS analysis did not indicate any noticeable changes in the compounds of all extracted essential oils. The results of this study showed that higher applied voltage can speed up OAHD and confirmed this emerging technology as a green technology (considering fossil fuels as the main source of electrical energy). Industrial relevance: The quality and quantity of essential oils extracted from herbs and other raw materials are affected by the extraction method. In this research different frequencies (25, 50 and 100 Hz) and also high and lowintensity OAHD (220 and 380 V, applicable in industries in many regions) of ohmic-assisted hydrodistillation (OAHD), as an innovative and emerging technology, were used and compared with traditional hydrodistillation in extraction of essential oils fromthe aerial parts of Mentha piperita L. (peppermint). All OAHD treatments were more economical and more environmentally friendly than hydrodistillation method. In addition, high intensity OAHD method not only can enhance the amount of extracted essential oil but also was the quickest method and consequently can be a valuable industrial alternative for extraction of essential oils.
    Full-text · Article · Jan 2015 · Innovative Food Science & Emerging Technologies
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    ABSTRACT: Hydrodistillation (HD) and steamdistillation (SD) are known to be the most prevalent methods of essential oils extraction. Despite many studies on extraction methods, there is no report on comparison of SD and HD and their extracted essential oils. In this study, SD was used for extraction of essential oils from Mentha piperita L. (peppermint), which is one of the medicinal plants of high consumption, and the results were compared with those of the HD. The results showed that although extraction with HD started sooner, both methods had the extraction time of about 1 hour. Scanning electron micrographs of mint leaves showed significant changes of glands after extraction in both methods. The required energy for extraction and kinetic parameters as well as quality of extracted essential oils where compared by physical constants and GC-MS analysis. The results of this study revealed that both HD and SD can be used as qualitative reference methods of extraction due to similarity of properties of extracted essential oils.
    Full-text · Article · Jan 2015 · International Food Research Journal
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    ABSTRACT: Sulfonamides are heat stable antibacterial drugs and residues of them in milk, increase the risk of human exposure and may cause food-borne illness. In this study, Fe3O4 magnetic nanoparticles (Fe3O4 MNPs) have been successfully used for extraction of sulfadiazine (SDZ) and sulfathiazole (STZ), from milk samples. The effect of magnetic adsorbents amount, volume of extracted milk and sample pH were investigated and optimized. The experimental results showed that suggested method possessed suitable analytical performance. Linearity was obtained over a concentration range of 0.25, 0.5, 1.0, 2.0 and 4 MRL, with regression coefficients ranging from 0.9985 to 0.9978 for SDZ and STZ. The limits of detection (LOD) and quantification (LOQ), for two analytes, were 10 and 30 ng mL-1, respectively. The proposed method was used to the analysis of different milk samples, and excellent recoveries in the range of 92.9 – 102.4% were obtained. These results indicated that the proposed method can be broadly used in monitoring of low concentrations of drugs in milk samples and open fascinating perspectives in future studies.
    Full-text · Article · Dec 2014 · Analytical methods
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    ABSTRACT: A functional polycation nanonetwork was developed for delivery of water soluble chemotherapeutic agents. The complexes of polyethyleneimine grafted methoxy polyethylene glycol (PEI-g-mPEG) and Zn(2+) were utilized as the micellar template for cross-linking with dithiodipropionic acid, followed by an acidic pH dialysis to remove the metal ion from the micellar template. The synthesis method was optimized according to pH, the molar ratio of Zn(2+), and the cross-link ratio. The atomic force microscopy showed soft, discrete, and uniform nano-networks. They were sensitive to the simulated reductive environment as determined by Ellman's assay. They showed few positive ζ potential and an average hydrodynamic diameter of 162±10 nm, which decreased to 49±11 nm upon dehydration. The ionic character of the nano-networks allowed the achievement of a higher-loading capacity of methotrexate (MTX), approximately 57% weight per weight, depending on the cross-link and the drug feed ratios. The nano-networks actively loaded with MTX presented some suitable properties, such as the hydrodynamic size of 117±16 nm, polydispersity index of 0.22, and a prolonged swelling-controlled release profile over 24 hours that boosted following reductive activation of the nanonetwork biodegradation. Unlike the PEI ionomer, the nano-networks provided an acceptable cytotoxicity profile. The drug-loaded nano-networks exhibited more specific cytotoxicity against human hepatocellular carcinoma cells if compared to free MTX at concentrations above 1 μM. The enhanced antitumor activity in vitro might be attributed to endocytic entry of MTX-loaded nano-networks that was found in the epifluorescence microscopy experiment for the fluorophore-labeled nano-networks.
    Full-text · Article · Jun 2014 · International Journal of Nanomedicine
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    ABSTRACT: Apple leather is made by dehydration of cooked fruit into leathery sheets. Mould growth and patulin production can occur in damaged apples or when fallen fruit is collected for apple leather processing. A survey was conducted to determine levels and dietary intake of patulin from apple leather marketed in Iran. Patulin was detected in all samples at concentrations ranging from <10 to 2559 µg/kg. Mean patulin concentration was 620 µg/kg and 91.4% of the samples had levels higher than 50 µg/kg. Estimated daily intake (EDI) of patulin for adults and children considering the mean concentration of patulin obtained in this study (620 µg/kg) were higher than the provisional maximum tolerable daily intake (PMTDI) of 0.4 µg/kg bw/day for patulin that has been established by the Joint FAO/WHO Expert Committee on Food Additives. This indicated the need for stricter control and improvement in processing techniques to reduce the incidence of patulin in apple leather.
    Full-text · Article · Jun 2014 · Food Additives and Contaminants: Part B Surveillance
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    ABSTRACT: β-site amyloid precursor protein cleaving enzyme (BACE-1) is a validated target for Alzheimer therapy due to its distinctive role in pathogenesis of AD. In the present contribution, a series of new 3,5-bis-N-(aryl/heteroaryl) carbamoyl-4-aryl-1,4-dihydropyridine structures were synthesized as BACE-1 inhibitors (6a-6n). In vitro BACE-1 inhibitory activities were determined by enzymatic fluorescence resonance energy transfer assay. Synthesized dihydropyridine (DHP) analogues exhibited weak to good inhibitory activities while 6i, 6n and 6a were found to be the most potent molecules with 83.76, 79.45 and 72.47 % BACE-1 inhibition at 10 μM, respectively. Structure binding/activity relationship elucidations revealed that superior BACE-1 inhibitory activities were observed for DHP derivatives bearing fused/non-fused thiazole groups and particularly 3,5-bis-N-(6-ethoxy-2-benzothiazolyl) moiety. Binding maps showed that enhanced activity may be attributed to the additional H-bond and hydrophobic interactions with S2-S3 subpockets of BACE-1.
    No preview · Article · Apr 2014 · Archives of Pharmacal Research
  • M. Alirezaei · M. Saeb · K. Javidnia · S. Nazifi · N. Khalighyan
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    ABSTRACT: A high level of total homocysteine (tHcy) is one hypothesis for the association of homocysteine with cerebrovascular diseases, neurodegenerative diseases and depression of mood. Thus, we examined whether oral betaine can act as a preventive agent in ethanol-induced hyperhomocysteinemia on the monoaminergic system. A total of 32 New Zealand White rabbits were divided into four groups (n=8); the Control group (C). The Ethanol group (E) was administered ethanol at a dosage of 4 g/kg daily. The Betaine group (B) received betaine at a dosage 1.5% (w/w) of the diet daily, and the Betaine and Ethanol group (BandE) was administered with the betaine group diet; after one hour the rabbits received ethanol at a dosage of 4 g/kg daily. Blood samples were taken in the morning of the day before beginning treatment (0.0 day) and on the 30th, 60th and 90th day of the treatment. Serum folate and vitamin B12 levels were determined using a radioimmunoassay, tHcy level of plasma was determined by homocysteine EIA kit, and 5- hydroxyindoleacetic acid (5-HIAA) of plasma was measured with HPLC-ECD. There was a significant negative correlation between 5-HIAA and tHcy in the E group (r=- 0.473, P=0.02), and compared to the E group the concentrations of 5-HIAA in the BandE group increased considerably (p<0.05). In contrast to the E group, significantly high concentrations of 5-HIAA were observed in the B and C groups. While the serum concentrations of vitamin B12 showed no significant difference in the BandE group on the 90th day compared to the control group, the serum concentrations of folate on the 90th day differed. However, no significant difference was observed between tHcy and gender. Overall, oral pretreatment with betaine significantly prevented ethanol-induced hyperhomocysteinemia, subsequently increasing 5-HIAA in the plasma as well as vitamin B12 and folate in the serum. Thus, betaine may be recommended as a pretreatment method for depressive patients with alcoholism.
    No preview · Chapter · Jan 2014
  • Mohsen Gavahian · Asgar Farahnaky · Katayoun Javidnia · Mahsa Majzoobi
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    ABSTRACT: In the extraction process of essential oils (EOs), one of the major problems of traditional methods is long extraction times. Ohmic-assisted hydrodistillation (OAHD) is a specific hydrodistillation (HD) technique utilizing ohmic heating to generate heat faster and in a different form from traditional methods. OAHD was applied as a novel technology for the isolation of EO from the aerial parts of Myrtus communis L. (myrtle) and the results were compared with those of the conventional HD. The results showed that OAHD method had an extraction time of 26.11 minutes while this value was more than 1 hour for HD for approximately the same amount of EO recovery (0.7% v/w). Gas chromatography–mass spectrometry (GC–MS) analysis did not indicate any noticeable changes in the constitutes of the EO obtained by OAHD in comparison with HD and the major compound of EOs was 1,8-cineol. The proposed method required considerably less energy per mL of EO extraction and the results of this study revealed the innovative OAHD as an environmentally friendly technology.
    No preview · Article · Aug 2013 · Journal of Essential Oil Research
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    Faraz Mojab · Azadeh Hamedi · Bahman Nickavar · Katayoun Javidnia
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    ABSTRACT: The chemical composition of the essential oil from the leaves of carrot (Daucus carota L. subsp. sativus (Hoffman.) Arcang. (Apiaceae) from Iran was analyzed by GC-FID and GC-MS. The oil yield obtained by hydrodistillation was 0.2 % (v/w). Ninety-one compounds, accounting for 97.1 % of the oil, were identified. The main class of the compounds was found to be monoterpenes (30.0 %), sesquiterpenes (27.8 %) and phenyl propanes (26.4 %). The major constituents were trans-anethole (23.5 %) and myrcene (14.5 %).
    Full-text · Article · Mar 2013 · Journal of essential oil-bearing plants JEOP
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    ABSTRACT: Asymmetric reduction of 8 prochiral β-ketoesers (la-h) mediated by Brassica rapa and Daucus carota fresh plant roots (sterile and non-sterile) and cell cultures were studied. Among different method evaluated, all reduced β-ketoesers in a Prelog manner. Therefore it is possible to prepare the (S)-secondary alcohols of (la-h) by reduction of corresponding prochiral ketone with these two biocatalysts. It was observed that reductive capacity of B. rapa was higher than D. carota generally, since high chemical and optical yields gained. After conducting the biotransformation in sterile condition, it was revealed that in most cases D.carota was more efficient in bioreductions than B.rapa. For better understanding, the biotransformation was run by cell cultures of these two vegetables. As could be expected no bioreduction was observed with B.rapa calli. These facts allow presenting role of microorganism in contribution to biotransformations by plant tissues and the necessity for sterilization prior to running the biotransformation.
    No preview · Article · Jan 2013 · International Journal of ChemTech Research

  • No preview · Article · Jan 2013
  • Mohsen Gavahian · Asgar Farahnaky · Katayoun Javidnia · Mahsa Majzoobi
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    ABSTRACT: Ohmic-assisted hydrodistillation (OAHD) is an advanced hydrodistillation (HD) technique utilizing ohmic heating process and could be considered as a novel method for the extraction of essential oils. One of the major problems with traditional methods is long extraction time. OAHD of essential oils from the aerial parts of Thymus vulgaris L. (common thyme) was studied and the results were compared with those of the conventional HD. The results showed that OAHD method had the extraction time of 24.75 min while this value was about 1 h for HD. Scanning electron micrographs of thyme leaves showed a sudden eruption of essential oil glands and their surrounding area for OAHD samples. GC–MS analysis did not indicate any noticeable changes in the compounds of the essential oils obtained by OAHD in comparison with HD. Results of this study introduced OAHD as a green technology because of less energy required per ml of essential oil extraction.Industrial relevanceThe quality and quantity of essential oils extracted from herbs and other raw materials are affected by the extraction method. In this research ohmic-assisted hydrodistillation (OAHD) was used as an advanced and novel hydrodistillation (HD) technique and compared with traditional hydrodistillation in extraction of essential oils from the aerial parts of Thymus vulgaris L. (common thyme). OAHD method was quicker, more economical and environmentally friendly than hydrodistillation method. The results of this study introduced OAHD as a green technology.
    No preview · Article · Apr 2012 · Innovative Food Science & Emerging Technologies
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    Ali Parsaeimehr · Elmira Sargsyan · Katayoun Javidnia
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    ABSTRACT: An experiment was conducted to estimate the influence of L-phenylalanine as a precursor on ephedrine and pseudo-ephedrine productions in Ephedra procera Fisch et C. A. Mey suspension culture. Our results indicated that, the highest amounts of 112.77 ± 0.54 μg·g -1 DW and 588.68 ± 1.84 μg·g -1 DW were achieved under the influence of 7.5 mM L-phenylalanine after 12 days for ephedrine and pseudo-ephedrine, respectively. Data also showed a positive correlation between the different levels of L-phenylalanine and the cell growth in the medium, but when the L-phenylalanine has reached 12.5 mM in the medium, the cell growth stopped.
    Full-text · Article · Jan 2012 · Russian Journal of Biopharmaceuticals
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    ABSTRACT: Ohmic-assisted hydrodistillation (OAHD) is a combination of ohmic heating and distillation, and could be considered as a novel method for the extraction of essential oils. Major problems with traditional methods are long extraction time and lower purity of the extract. In this study, OAHD was applied as an economic and green technology for the extraction of essential oils from Zataria multiflora Boiss. (Shirazi thyme) aerial parts and the results were compared to those obtained from hydrodistillation (HD) as a conventional method. The results showed that OAHD method had the extraction time of 32.21 ± 2.59 min while this value was about 57.21 ± 2.33 min for hydrodistillation (HD). Scanning electron micrographs of thyme leaves showed a sudden eruption of essential oil glands and their surrounding area for OAHD samples. GC–MS analysis indicated that both methods of OAHD and HD can extract the same compounds.
    No preview · Article · Dec 2011 · International Journal of Food Science & Technology
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    ABSTRACT: The antioxidant activities of the methanolic extracts of Ephedra laristanica growing in Iran were evaluated using ferric reducing antioxidant power (FRAP) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. FRAP values 2.1 mmol eq quercetin/g extracts, and IC50 values in the DPPH assay 4.6 mg/mL. This plant showed the high antioxidant activities. FRAP and DPPH assay results showed good correlations with the total phenolic contents of the plants, measured by the Folin- Ciocalteau assay (r2 = 0.926 and 0.913, respectively, p < 0.0001). The antimicrobial capacity was screened against Gram positive and Gram negative bacteria, and fungi. The extract inhibited the growth of Gram negative bacteria, being Escherichia coli the most susceptible one with MIC of 32 �g/mL for the extract. The results obtained indicate that E. laristanica may become important in the obtainment of a noticeable source of compounds with health protective potential and antimicrobial activity.
    Full-text · Article · Oct 2011 · Journal of medicinal plant research
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    ABSTRACT: The antimicrobial capacity of the methanolic extracts of Ephedra sarcocarpa growing in Iran is screened against gram-positive and gram-negative bacteria and fungi. The extract inhibited the growth of Gram-negative bacteria, being Pseudomonas aeroginosa the most susceptible one with MIC of 16 μg/mL for the extract. The antioxidant activities are evaluated using ferric reducing antioxidant power (FRAP) and 2, 2 diphenyl-1 picrylhydrazyl (DPPH) free radical scavenging assays. FRAP values 2.1 mmol eq quercetin/g extracts and IC50 values in the DPPH assay 4.6 mg/mL. This plant had shown the high antioxidant activities. FRAP and DPPH assay results were shown good correlations with the total phenolic contents of the plants, measured by the Folin-ciocalteau assay: (r2 = 0.920 and 0.893, respectively, p < 0.0001).
    Full-text · Article · Sep 2011
  • M Farjam · A Rustaiyan · AR Jassbi · K Javidnia

    No preview · Article · Aug 2011 · Planta Medica
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    A. Alizadeh · M. Khosh-Khui · K. Javidnia · O. Firuzi · S.M. Jokar
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    ABSTRACT: The chemical composition of essential oil isolated by hydrodistillation from the aerial part of Origanum majorana L. cultivated in Iran was determined using Gas Chromatography (GC) and Gas Chromatography/Mass Spectrometry (GC/MS). The main components of the oil in Origanum majorana, were trans Sabinene hydrate (39.2% ± 3.7), Terpinene-4-ol (16.8% ± 2.3), cis-Sabinen hydrate (7.5% ± 0.4), δ-Terpinene (6.6% ± 0.9), Sabinene (5.2% ± 0.1), α- Terpineol (4.6 ± 0.5), α- Phellandrene (3.6 ± 0.4), p-Cymene (2.6 ± 0.8) and ß-Caryophyllene (2.5 ± 0.3). The total phenolic contents and the antioxidant activity of plant extract were determined, respectively, by Folin-Ciocalteau and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays. Plant extract of Origanum majorana had more phenolic content and radical scavenging effect. A significant correlation was found between phenolic contents and antioxidant activity (R 2=0.98). The results showed that Origanum majorana possesses significant sources of natural antioxidant properties for culinary and possible medicinal use.
    Full-text · Article · Jul 2011 · Advances in Environmental Biology