Jie Yang

University of Queensland, Brisbane, Queensland, Australia

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Publications (48)222.06 Total impact

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    ABSTRACT: This study investigated the priming effect of sphingosine 1-phosphate (S1P) on formyl-Met-Leu-Phe-OH (fMLP)-activated neutrophils, by specific analysis of the neutrophil respiratory burst and the signaling pathway involved in S1P activity. The neutrophil respiratory burst was indirectly detected by the cytochrome c reduction method and the dihydrorhodamine 123 staining method. The signal transduction pathways of neutrophil respiratory burst primed by S1P were detected by Western blotting. Our results showed that the S1P receptors (S1PRs) 1, 4 and 5 were the predominantly expressed neutrophil S1PRs at the cDNA level. After pretreatment with S1P, the fMLP-activated neutrophils released increased levels of superoxide anions. PI3K and Akt proteins were involved in the signaling pathway of the neutrophil respiratory burst primed by S1P. The results indicate that S1P is a new priming reagent for neutrophils and primes the respiratory burst of fMLP-activated neutrophils. S1P interacts with its receptors on neutrophils, resulting in NADPH oxidase activation by the PI3K-Akt cell signaling pathway and induction of the neutrophil respiratory burst. © 2015 S. Karger AG, Basel.
    No preview · Article · Apr 2015 · Acta Haematologica
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    ABSTRACT: In this work, a 3D geometric electron tomography (3D-GET) approach has been developed, through which a new insight into cage-type ordered mesostructures and their pore sizes has been obtained. It is demonstrated that the accurate pore diameter, especially for cage-type cubic mesoporous materials, can only be determined through our 3D-GET approach by considering the pore geometry is a real 3D space. We use the 3D-GET method to revisit the applicability of different models for the pore size calculation in nitrogen adsorption analysis. Different from the overwhelming understanding that the nonlocal density functional theory (NLDFT) and Derjaguin-Broekhoff-de Boer (BdB) modelare recommended to calculate the pore size of cage-type cubic mesoporous materials while the Barret-Joyner-Halenda (BJH) model should not be used, a new understanding is gained through this study. The choice of a suitable model for pore size determination depends on the precise pore structure. For a cage-type cubic mesoporous material with an fcc symmetry and large entrance connecting the cages, BJH model is more accurate while the other two methods both overestimate the pore size (up to 40% higher). The DFT model is more appropriate when the pore shape is a perfect sphere than BJH and BdB model which underestimates and overestimates the pore size, respectively. It is our opinion that the unique 3D-GET approach should be used to revisit a vast number of large-pore cubic mesoporous materials to provide genuine structural information.
    No preview · Article · Feb 2015 · Langmuir
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    Jie Yang · Yiming Yang · Huahua Fan · Hejian Zou
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    ABSTRACT: TGF-β-induced regulatory T cells (iTregs) retain Foxp3 expression and immune-suppressive activity in collagen-induced arthritis (CIA). However, the mechanisms whereby transferred iTregs suppress immune responses, particularly the interplay between iTregs and dendritic cells (DCs) in vivo, remain incompletely understood. In this study, we found that after treatment with iTregs, splenic CD11c(+)DCs, termed "DCiTreg," expressed tolerogenic phenotypes, secreted high levels of IL-10, TGF-β, and IDO, and showed potent immunosuppressive activity in vitro. After reinfusion with DCiTreg, marked antiarthritic activity improved clinical scores and histological end-points were observed. The serological levels of inflammatory cytokines and anti-CII antibodies were low and TGF-β production was high in the DCiTreg-treated group. DCiTreg also induced new iTregs in vivo. Moreover, the inhibitory activity of DCiTreg on CIA was lost following pretreatment with the inhibitor of indoleamine 2,3-dioxygenase (IDO). Collectively, these findings suggest that transferred iTregs could induce tolerogenic characteristics in splenic DCs and these cells could effectively dampen CIA in an IDO-dependent manner. Thus, the potential therapeutic effects of iTregs in CIA are likely maintained through the generation of tolerogenic DCs in vivo.
    Preview · Article · Oct 2014 · Research Journal of Immunology
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    ABSTRACT: A novel approach has been developed to prepare polyethyleneimine functionalized hybrid silica spheres with a diameter of ~10 nm, which show excellent delivery efficiency of siRNA into osteosarcoma cancer cells and human colon cancer cells with a significant cell inhibition comparable to commercial agents.
    No preview · Article · Sep 2014 · ACS Applied Materials & Interfaces
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    ABSTRACT: Mesoporous silica nanoparticles (MCM-41) with different surface chemistry were used as carrier system to study its influence on drug delivery and anticancer activity of curcumin (CUR). CUR was encapsulated in pristine MCM-41 (hydrophilic and negatively charged), amino functionalized MCM-41 (MCM-41-NH2 which is hydrophilic and positively charged) and methyl functionalized MCM-41 (MCM-41-CH3 which is hydrophobic and negatively charged) and evaluated for in vitro release and cell cytotoxicity in human squamous cell carcinoma cell line (SCC25). Various techniques were employed to evaluate the performance of these materials on cellular uptake and anticancer activity in SCC25 cell line. Both positively and negatively charged surface demonstrated enhanced drug release and anticancer activity compared to pure curcumin. Positively charged nanoparticles showed higher cell uptake compared to negatively charged nanoparticles owing to its electrostatic interaction with cells. However, hydrophobic surface modified nanoparticles (MCM-41-CH3) showed no improvement in drug release and anticancer activity due to its poor wetting effect. Cell cycle analysis and cell apoptosis studies revealed different pathway mechanisms followed by the positively and negatively charged nanoparticles but exhibiting similar anticancer activity in SCC25 cells.
    No preview · Article · Aug 2014 · Molecular Pharmaceutics
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    ABSTRACT: Objective: To explore the biological characteristics and the immuno-suppression function of tolerogenic dendritic cells (tDC) induced by tacrolimus. Methods: Human monocytes derived from peripheral blood were cultured in the cGMP-compliant CellGro DC medium supplemented with GM-CSF and IL-4 to obtain dendritic cells (DCs), and 0.1 μmol/L immunosuppressive drug tacrolimus was added to the culture medium at the third and fifth day to obtain tDCs. The molecular markers of them and the livability were assayed by flow cytometry. Then the tolerance functionality of tDCs induced by many agents and these tDCs modulated allogeneic CD4 T cells was determined via CFSE proliferation assay. And the research also analyzed the biological characters and immunosuppression function of tDCs induced by tacrolimus after storing. Results: tDCs induced by tacrolimus exhibit a typical tolerogenic phenotype, whose level of costimulatory molecules CD80, CD83, CD86 and HLA-DR is (2.95 ± 1.32)%, (2.33 ± 1.60)%, (90.02 ± 7.42)% and (91.80 ± 6.18)%, respectively. It's survival rate was (85.2 ± 4.72)%. And immunosuppressive drugs didn't influence the differentiation of tDCs from monocytes. tDCs induced by immunosuppressive drugs dexamethasone, cyclosporin A and tacrolimus had lower immunogenic than control DCs as CD4+ T proliferation rate of tDCs induced by tacrolimus is 0.42% and could not primed allogeneic CD4+ T cells proliferation. Functional analyses showed that tDCs induced by tacrolimus can more effectively suppressed mature DC-induced T cell proliferation than other tDCs, whose inhibition rate can reach (67.01 ± 19.73)%. Importantly, tDCs induced by tacrolimus had phenotypical and functional stability after storing. Conclusion: tDCs induced by tacrolimus with tolerance functionality are a promising cellular therapeutic for immunomodulation.
    No preview · Article · Jun 2014 · Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
  • Yana Ren · Yiming Yang · Jie Yang · Rufeng Xie · Huahua Fan
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    ABSTRACT: Background: Tolerogenic dendritic cells (tDCs) can be generated in vitro by a variety of methods, including genetic or pharmacological modification. DCs that were modified by the immunosuppressive drug tacrolimus were considered to be endowed with tolerogenic functions. Study design and methods: DCs derived from human monocytes were induced in vitro by GM-CSF/IL-4 with tacrolimus. The phenotype and production of cytokines in these DCs were analyzed. The functionality of tDCs modified by tacrolimus was subsequently determined via a CFSE proliferation assay. The severity of arthritis was monitored in CIA mice after treatment with tDCs modified by tacrolimus. Results: tDCs that were modified by tacrolimus exhibited an immature phenotype. The expression of mRNA encoding IL-10 and TGF-β increased after 12h of tacrolimus stimulation, with the strongest responses being observed after 24h. The mRNA was further upregulated after tDCs were treated with LPS and IFN-γ. tDCs secreted more IL-10 and less TNF-α and had a reduced ability to activate allo-CD4(+)CD25(-) T cells. These cells suppressed mDC-induced-proliferation of CD4(+)CD25(-) T cells and produced less TNF-α and IFN-γ but increased the level of IL-10 than imDCs. Treatment of arthritic mice with tDCs modified by tacrolimus significantly inhibited the severity and progression of the disease. tDC treatment also altered the proportion of the Th1 and Th17 populations in the spleen. Conclusions: tDCs modified by tacrolimus suppressed CD4(+) T cell proliferation and inhibited collagen-induced arthritis. These results suggest the potential use of tDCs as a therapeutic approach for autoimmune arthritis.
    No preview · Article · May 2014 · International Immunopharmacology
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    ABSTRACT: Novel mesoporous silica nanoparticles (MSNs) responsive to multiple biological stimuli (pH and enzymes) were prepared through conjugation with a structure modified soy protein isolate. The particles show an extremely high pro-drug (sulfasalazine) loading with programmable drug release in simulated gastrointestinal fluid.
    No preview · Article · Mar 2014 · Chemical Communications
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    ABSTRACT: Novel composites of iron oxide encapsulated in macroporous silica with excellent arsenic adsorption performance have been successfully developed. Macroporous silica foams with large pore sizes of ≈100 nm and a high pore volume of 1.6 cm3 g−1 are chosen as the porous matrix. Electron tomography technique confirms that γ-Fe2O3 nanoparticles with an average particle size of ≈6 nm are spatially well-dispersed and anchored on the pore walls at even a high γ-Fe2O3 content of 34.8 wt%, rather than forming aggregates inside the pores or on the external surface. The open large-pore structure, high loading amount, and the non-aggregated nature of γ-Fe2O3 nanoparticles lead to increased adsorption sites and thus high adsorption capacities of both As (V) and As (III) without pre-treatment (248 and 320 mg g−1, respectively). Moreover, the composites can reduce the concentration of both As (V) and As (III) from 100 to 2 μg L−1. It is also demonstrated that the composites can be applied in a household drinking water treatment device, which can continuously treat 20 L of wastewater containing As (V) with the effluent concentration lower than the World Health Organization standard.
    Full-text · Article · Mar 2014 · Advanced Functional Materials
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    ABSTRACT: The development of functional nanocarriers that can enhance the cellular delivery of a variety of nucleic acid agents is important in many biomedical applications such as siRNA therapy. We report the synthesis of large pore mesoporous silica nanoparticles (LPMSN) loaded with iron oxide and covalently modified by polyethyleneimine (denoted PEI-Fe-LPMSN) as carriers for gene delivery. The LPMSN have a particle size of ∼200 nm and a large pore size of 11 nm. The large pore size is essential for the formation of large iron oxide nanoparticles to increase the magnetic properties and the adsorption capacity of siRNA molecules. The magnetic property facilitates the cellular uptake of nanocarriers under an external magnetic field. PEI is covalently grafted on the silica surface to enhance the nanocarriers' affinity against siRNA molecules and to improve gene silencing performance. The PEI-Fe-LPMSN delivered siRNA-PLK1 effectively into osteosarcoma cancer cells, leading to cell viability inhibition of 80%, higher compared to the 50% reduction when the same dose of siRNA was delivered by a commercial product, oligofectamine.
    Full-text · Article · Feb 2014 · Nanotechnology
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    ABSTRACT: A facile and controllable interface-directed co-assembly (IDCA) approach is developed for the first time to synthesize uniform discrete mesoporous silica particles with a large pore size (ca. 8 nm) by using 3-dimensional macroporous carbon (3DOMC) as the nanoreactor for the confined co-assembly of template molecules and silica source. By controlling the amount of the precursor solution and using Pluronic templates with different compositions, mesoporous silica particles with diverse morphologies (spheres, hollow spheres, and hemispheres) and different mesostructure (e.g. 2-D hexagonal and 3D face centered cubic symmetry), high surface area of about 790 m2/g, and large pore volume (0.98 cm3/g) can be synthesized. The particle size can be tunable from sub-micrometer to micrometer regimes by changing the macropore diameter of 3DOMC. Importantly, this synthesis concept can be extended to fabricate multifunctional mesoporous composite spheres with a magnetic core and a mesoporous silica shell, large saturated magnetization (23.5 emu/g), high surface area (280 m2/g). By using the magnetic mesoporous silica spheres as a magnetically recyclable absorbent, a fast and efficient removal of microcystin from water is achieved, and they can be recycled for 10 times without a significant decrease of removal efficiency for microcystin.
    No preview · Article · Jan 2014 · Journal of the American Chemical Society
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    ABSTRACT: A facile approach has been developed to prepare ultra-small hybrid silica spheres with a diameter of ∼10 nm, which show highly enhanced penetration ability in 3D glioma spheroids compared to conventional mesoporous silica nanoparticles.
    Preview · Article · Dec 2013 · Chemical Communications
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    ABSTRACT: Objectives: To determine the effect of nicotine stimulation on collagen-induced arthritis (CIA), especially on Th17 cells, and the influence of activated acetylcholine receptor signaling on the induction and function of in vitro-cultured Th17 cells. Methods: Mice were divided into control and experimental (nicotine) group, and PBS or nicotine-PBS was orally administered from Day 21 to Day 28. Phenotypic changes in spleen CD4(+) cells were measured by flow cytometry. α7nAChR expression in Th17 cells was detected using flow cytometry, western blotting and real-time PCR. Purified Th17 cells were further stimulated with nicotine. The cytometric bead array (CBA) assay was employed to measure TNF-α levels in mice serum and IL-17A levels in the supernatants of nicotine-treated cell cultures. Results: Compared with their counterparts, mice receiving oral nicotine showed a delayed progress of arthritis and more attenuated signs of histological changes. Moreover, serum TNFα levels were lower in the nicotine-treated group. Spleen IL-17 level of nicotine-treated mice was lower than that of the control group, and the mRNA expression of pro-inflammatory cytokines (IL-17A and IL-6) in splenocytes were also lower than that of the control group. α7nAChR expression was detected on in vitro-cultured IL-17A(+) cells. Cells treated with 10 (- 6) M nicotine expressed lower IL-17A levels. Similarly, supernatants from nicotine-treated cell cultures also showed lower IL-17A levels. Conclusions: Nicotine stimulation attenuated signs and severity of arthritis in mice. Activation of nicotine acetylcholine receptors on in vitro-cultured Th17 cells decreased their pro-inflammatory function, which may play a potential role in alleviating arthritis in mice.
    No preview · Article · Dec 2013 · Modern Rheumatology
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    ABSTRACT: We present an approach to optimize the MapReduce architecture, which could make heterogeneous cloud environment more stable and efficient. Fundamentally different from previous methods, our approach introduces the machine learning technique into MapReduce framework, and dynamically improve MapReduce algorithm according to the statistics result of machine learning. There are three main aspects: learning machine performance, reduce task assignment algorithm based on learning result, and speculative execution optimization mechanism. Furthermore, there are two important features in our approach. First, the MapReduce framework can obtain nodes' performance values in the cluster through machine learning module. And machine learning module will daily calibrate nodes' performance values to make an accurate assessment of cluster performance. Second, with the optimization of tasks assignment algorithm, we can maximize the performance of heterogeneous clusters. According to our evaluation result, the cluster performance could have 19% improvement in current heterogeneous cloud environment, and the stability of cluster has greatly enhanced.
    No preview · Article · Dec 2013 · The Journal of China Universities of Posts and Telecommunications
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    ABSTRACT: Novel silica nanoparticles mimicking virus surface topography are prepared. It is demonstrated that increases in nanoscale surface roughness promote both binding of biomolecules and cellular uptake; thus, the cellular delivery efficiency is significantly increased (see picture; scale bars 20 μm).
    No preview · Article · Nov 2013 · Advanced Materials
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    ABSTRACT: A new strategy to synthesize nanoparticles with enhanced cellular delivery efficiency is presented by Chengzhong Yu and co-workers on page 6233. Silica nanoparticles mimicking virus surface topography exhibit increased cellular uptake performance and improved binding capacity towards biomolecules (e.g., genetic molecules, proteins); thus the cellular delivery performance can be improved. This understanding is important in the rational design of new cellular delivery vectors for a range of bioapplications.
    Full-text · Article · Nov 2013 · Advanced Materials
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    ABSTRACT: Tolerogenic dendritic cells (tDCs) are immunosuppressive cells with potent tolerogenic ability and are promising immunotherapeutic tools for treating rheumatoid arthritis (RA). However, it is currently unknown whether allogeneic tDCs (allo-tDCs) induce tolerance in RA, and whether the numbers of adoptively transferred allo-tDCs, or the requirement for pulsing with relevant auto-antigens are important. tDCs were derived from bone marrow precursors of C57BL/B6 mice, which were induced in vitro by GM-CSF, IL-10 and TGF-β1. Collagen-induced arthritis (CIA) was modeled in D1 mice by immunization with type II collagen (CII) to test the therapeutic ability of allo-tDCs against CIA. Clinical and histopathologic scores, arthritic incidence, cytokine and anti-CII antibody secretion, and CD4(+)Th subsets were analyzed. tDCs were characterized in vitro by a stable immature phonotype and a potent immunosuppressive ability. Following adoptive transfer of low doses (5×10(5)) of CII-loaded allo-tDCs, a remarkable anti-arthritic activity, improved clinical scores and histological end-points were found. Serological levels of inflammatory cytokines and anti-CII antibodies were also significantly lower in CIA mice treated with CII-pulsed allo-tDCs as compared with allo-tDCs. Moreover, treatment with allo-tDCs altered the proportion of Treg/Th17 cells. These findings suggested that allo-tDCs, especially following antigen loading, reduced the severity of CIA in a dose-dependent manner. The dampening of CIA was associated with modulated cytokine secretion, Treg/Th17 polarization and inhibition of anti-CII secretion. This study highlights the potential therapeutic utility of allo-tDCs in autoimmune arthritis and should facilitate the future design of allo-tDC immunotherapeutic strategies against RA.
    Preview · Article · Oct 2013 · PLoS ONE
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    ABSTRACT: Delicate α-Fe2O3 multi-shelled hollow spheres have been prepared by a simple and scalable spray drying method followed by annealing in air. The resulting material shows high specific capacity, good cycling stability, and excellent rate performance in lithium ion battery applications.
    No preview · Article · Aug 2013 · Chemical Communications
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    ABSTRACT: The enrichment of peptides is a key technique in mass spectrometry based proteomics and peptidomics. Tailored design of mesoporous materials with optimum pore size for highly-efficient enrichment of target molecules is a challenging issue. Herein, a series of periodic mesoporous organosilicas (PMOs) are synthesized with the same structure symmetry (p6mm) and similar morphology, while the pore sizes are finely adjusted from 2.6 to 7.3 nm. Their enrichment performance for a standard E7 peptide (molecular weight 1120.6 Da) is investigated via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). It is found that PMO with the mesopore size of 5.8 nm exhibits the highest enrichment performance towards the E7 peptide. Moreover, a block copolymer (Brij 78) with the similar molecular weight (1150.8 Da) to E7 is also used to further study the influence of mesopore size upon the enrichment efficiency. It is shown that PMO with the pore size of 5.8 nm still holds the best enriching ability towards Brij 78 at low concentrations. The adsorption capacity of the PMOs for Brij 78 are further studied at high concentrations, showing a dependence on both the pore volume and pore size. This research may shed light on advanced enrichment and analysis of various peptides and polymers using designed nanoporous materials, an important topic in both material and biological science.
    No preview · Article · Jun 2013 · RSC Advances
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    ABSTRACT: A facile spray drying technique has been developed for large-scale and template-free production of nanoporous silica with controlled morphology, large pore size, and high pore volume, using commercially available fumed silica, Aerosil 200, as a sole precursor. This approach can be applied to the preparation of functional nanoporous materials, in this study, lanthanum oxide functionalised silica microspheres by introducing lanthanum nitrate in situ during the spray drying process and followed by a post-calcination process. The resultant lanthanum functionalised Aerosil microspheres manifest high phosphate adsorption capacity (up to 2.317 mmol g(-1)), fast kinetics, and excellent adsorption performance at a low phosphate concentration (1 mg L(-1)). In virtue of the easy and scalable synthesis method, low cost and high performances of the product, the materials we reported here are promising for water treatment. Our approach may be general and extended to the synthesis of other functional nanoporous materials with versatile applications.
    Full-text · Article · Jun 2013 · Nanoscale

Publication Stats

563 Citations
222.06 Total Impact Points

Institutions

  • 2011-2014
    • University of Queensland
      • Australian Institute for Bioengineering and Nanotechnology
      Brisbane, Queensland, Australia
  • 2008-2014
    • Fudan University
      • Department of Chemistry
      Shanghai, Shanghai Shi, China
  • 2011-2013
    • Beijing University of Posts and Telecommunications
      • Beijing Key Laboratory of Network System Architecture and Convergence
      Peping, Beijing, China
  • 2011-2012
    • East China Normal University
      • Institute of Biomedical Sciences and School of Life Sciences
      Shanghai, Shanghai Shi, China