K B Thomas

Christian-Albrechts-Universit├Ąt zu Kiel, Kiel, Schleswig-Holstein, Germany

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Publications (3)5.55 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: von Willebrand factor (vWF) antigen (vWF:Ag) and vWF-collagen binding activity (vWF:CBA) were measured in plasma by parallel quantitative ELISAs in normal newborns and infants (n = 71). The medians for vWF:Ag (IU/ml) and vWF:CBA (U/ml), respectively, were 1.46 and 1.91 for 2-7 day-old (n = 43), 1.22 and 1.40 for 2-4 week-old (n = 14), 1.22 and 1.15 for 2-6-month-old (n = 14) infants and 0.98 and 1.08 (n = 36) in normal adults. Elevated levels of vWF:Ag, but particularly vWF:CBA were seen for up to 4 weeks of life reaching adult levels between 2 and 6 months of life. The elevated levels of the vWF parameters indicate that caution should be exercised when interpreting laboratory data and diagnosing von Willebrand disease in newborns and young infants and warrant the use of age-specific reference ranges. The efficient haemostasis observed during early neonatal life may in part be due to the increased ability of vWF to interact with collagen.
    No preview · Article · Jul 1995 · Acta Paediatrica
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    ABSTRACT: Von Willebrand disease (vWD), a disorder of primary hemostasis, represents the most frequently inherited bleeding diathesis, It is caused by a quantitative reduction or a qualitative abnormality, or both, of von Willebrand factor (vWF), a glycoprotein normally found in plasma, endothelial cells, subendothelial cell space, megakaryocytes and platelets. Patients with vWD represent a heterogeneous group with different phenotypes and with clinical symptoms that vary in severity. Many of the described types and subtypes of vWD are caused by mutations and aberrations of the vWF gene. The aim of this article is to highlight the impact of gene analysis on the current knowledge of the inheritance of vWD as well as on the structure-function relationship of vWF, and to focus on the available diagnostic laboratory tests and treatment options for patients with vWD, especially in childhood.
    No preview · Article · Feb 1995 · Seminars in Thrombosis and Hemostasis
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    ABSTRACT: The ability of von Willebrand factor (vWF) to bind to immobilised collagen, i.e. the vWF-collagen binding activity (vWF:CBA), was used in an ELISA which allows the determination of vWF function. The ELISA for vWF:CBA has been refined such that the same sample dilutions, buffers and instruments could be used in parallel with the ELISA for vWF antigen (vWF:Ag), thereby allowing differentiation of patients with von Willebrand disease into types I and II. The vWF:CBA assay is sensitive, fast and simple, requiring standard ELISA equipment and minimal amount of sample.
    No preview · Article · Jan 1994

Publication Stats

56 Citations
5.55 Total Impact Points

Institutions

  • 1995
    • Christian-Albrechts-Universit├Ąt zu Kiel
      Kiel, Schleswig-Holstein, Germany