J Leiva

Hospital Universitario Virgen de las Nieves, Granata, Andalusia, Spain

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Publications (7)11.15 Total impact

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    ABSTRACT: The ability of in vitro and in vivo detection of Brucella spp. with the Bact/Alert system was studied. Three strains of Brucella melitensis and two of Brucella abortus were used. Different dilutions of the five strains were performed in trypticase soy broth (TSB), achieving concentrations of 1 cfu/ml, 5 cfu/ml, 10 cfu/ml and 100 cfu/ml. Ten ml of each dilution and strain were inoculated into 5 aerobic bottles Bact/Alert and 5 biphasic Hemóline bottles. Furthermore, over a 9 month period, 8,216 bottles of Bact/Alert bottles from hospitalized patients and from the emergency department were processed in the authors' laboratory. The mean detection time for Brucella growth was from 2 to 3 days with the Bact/Alert system, and 14 days in the biphasic bottles. Former bottles processed in the authors' laboratory, 11 aerobic bottles belonged to 5 patients in whom brucelosis was confirmed by bloodculture. The Bact/Alert system detected Brucella melitensis in only on bottle at 2.9 days of incubation. In 7 bottles Bact/Alert detected B. melitensis by a blind pass of these bottles at 10 to 20 days of incubation. These results suggest that the Bact/Alert system does not totally solve the diagnosis of brucellosis. Blind passes of the bloodcultures are required.
    No preview · Article · Jan 1995 · Enfermedades Infecciosas y Microbiología Clínica
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    ABSTRACT: The presence of circulating immunocomplexes (ICs) was studied in 57 patients diagnosed with acute brucellosis. Total ICs were detected directly with nephelometry and Brucella-specific ICs by immunoblotting. The frequency of total ICs was 75.4% and Brucella-specific ICs were found in 50.9%. The most frequent protein antigens were those against 66 kDa and 21 kDa. No Brucella-specific ICs were found in sera from any of the control subjects.
    No preview · Article · Sep 1994 · Serodiagnosis and Immunotherapy in Infectious Disease
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    ABSTRACT: Cysticercosis, a rare disease in southern Spain, can have serious consequences if it arises in intracerebral or intraocular locations. The diagnosis requires both imaging techniques and serological tests. We analysed the immunoblot technique with an acrylamide gel gradient as a method for making a specific diagnosis. The samples consisted of 16 sera and nine cerebrospinal fluids from 14 patients in whom cysticercosis was clinically suspected, and 11 sera from patients with other parasitic infections as a control group. Bands with apparent molecular weights of 200, 150, 116, 80, 65, 40, 35, 27 and 16 kDa were obtained; the low (35, 27 and 16 kDa) and high molecular weight bands (116, 150, 200), appeared in the majority of samples. In comparison with linear gel immunoblotting assays, gel gradient assays had the advantage of detecting a wider range of molecular weights.
    No preview · Article · Oct 1993 · Serodiagnosis and Immunotherapy in Infectious Disease
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    ABSTRACT: Evaluation of two serological techniques for diagnosis cysticercosis: complement fixation reaction and Western blot. Comparative study that includes 49 sera samples and 30 CSF samples belonging to 35 patients with clinical diagnosis of cysticercosis of the CNS. As a control group we used 10 sera samples of patients with Trichinella spiralis infection, 33 sera samples of patients with hydatid cyst disease, 9 sera samples from patients with oxyuriasis, 52 sera samples of healthy blood donors and 22 additional CSF samples from patients with different CNS disorders. Thirty-one samples were positive by Western blot technique and 21 samples by complement fixation reaction technique. Agreement between both techniques was 76%. Gathering clinical and laboratory information we believe that a total of 11 patients had cysticercosis of the CNS (33 samples), and three additional patients remain with indeterminate diagnosis. We have also detected cross-reactions with Western blot techniques (reactivity to more than one band) in 43% of patients with hydatid disease and in 20% of patients with Trichinella spiralis infection, but none in oxyuriasis patients. The CFR technique had cross-reaction with 33% of sera samples from hydatid disease patients and 20% of those from oxyuriasis patients. No cross-reactions were seen in any of control patients with both techniques. The two different techniques tested can be of help, together with clinical and radiology data, in the diagnosis of cysticercosis of the CNS. However, both techniques showed strong cross-reactions with hydatid disease.
    No preview · Article · Dec 1991 · Enfermedades Infecciosas y Microbiología Clínica
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    ABSTRACT: In order to establish the possible influence of Brucella melitensis rough strains protein extracts contamination using LPS (R) in the immunoblotting evaluation of serologic response, we have studied sera samples from 41 patients with acute brucellosis before treatment was started and also from 62 healthy individuals (control group). A whole-cell B. melitensis 115 strain extract with an without LPS (R) was used. Extraction was carried using ether-chloroform of oil-phenol. Overall, more than 50% of patients showed an antibody response (protein bands 85, 53, 50, 47, 41, 38 and 28 kD) to both antigenic products. Less than 6.4% of the control group sera showed response to any of these protein bands. There were no significant differences in the frequency of response development against these proteins using the two antigen types. However, there was some disagreement when each individual band response was compared to its homologous band.
    No preview · Article · Nov 1991 · Enfermedades Infecciosas y Microbiología Clínica
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    ABSTRACT: Pre- and post-treatment measurements of C-reactive protein (CRP) in 85 patients with acute brucellosis were useful in predicting the disease's evolution and response to treatment. A positive value for CRP (greater than 1 microgram/dl) was significantly associated with an unfavorable course, with a negative predictive value of 0.95.
    No preview · Article · May 1990 · Diagnostic Microbiology and Infectious Disease
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    ABSTRACT: We have evaluated with Western blotting the serologic response of 144 patients with acute brucellosis and 62 healthy controls to identify a useful antibody pattern to confirm the diagnosis of acute brucellosis. The antigen that we used was a protein extract of whole cells of the strain 115 (rough) of B. melitensis. Patients sera were obtained at the time of diagnosis and before the start of specific therapy. There was antibody response to 21 protein bands. The molecular weights (MW) and frequencies of the most significant bands were the following: 85 Kd (60.4%), 70 Kd (54.1%), 53 Kd (63.9%), 50 Kd (65.3%), 47 Kd (71.5%), 41 Kd (74.3%), 38 Kd (68.7%), 33 Kd (56.2%), 28 Kd (52.8%). At the time of diagnosis, 90.3% of the patients with acute brucellosis had antibody response to 2 or more protein bands of Mw of 47 Kd, 41 Kd, 38 Kd and 33 Kd. In none of the 62 negative control sera there was a response to more than one of the mentioned bands.
    No preview · Article · Feb 1990 · Enfermedades Infecciosas y Microbiología Clínica